We narrowed to 16,886 results for: Por

1. pLHCX-FLAG-mPKM1

   Plasmid

   #44240

   Depositor

   Matthew Vander Heiden, Lewis Cantley

   Article

   Christofk et al Nature. 2008 Mar 13;452(7184):230-3. doi

   Insert

   Pyruvate Kinase M1 (Pkm Mouse)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 10, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pSLIK 3xFLAG-CSE1L zeo

   Plasmid

   #192320

   Purpose

   Lentiviral expression vector for an inducible 3xFLAG-CSE1L

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   CSE1L (CSE1L Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ER-NFAST

   Plasmid

   #233596

   Purpose

   Expression of NFAST on the ER membrane

   Depositor

   Riccardo Filadi

   Article

   Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

   Insert

   NFAST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Myc-βarrestin2-LOV-Turbo_pCDNA3

   Plasmid

   #199671

   Purpose

   expresses Myc-βarrestin2-LOV-Turbo in mammalian cells

   Depositor

   Alice Ting

   Article

   Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

   Insert

   LOV-Turbo
   

   Use

   Tags

   Myc and βarrestin2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CAGGS-EGFP-NLS-3XFLAG-V5

   Plasmid

   #196090

   Purpose

   Expresses gfp in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

   Depositor

   Mauro Calabrese

   Article

   Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

   Insert

   GFP
   

   Use

   Tags

   C-terminal 3xFLAG and C-terminal V5

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAGGs

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-hSyn-DIO-GRAB_eCB2.0

   Plasmid

   #164606

   Purpose

   Express the endocannabinoid sensor GRAB_eCB2.0 in Cre positive neurons

   Depositor

   Yulong Li

   Article

   Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

   Insert

   Endocannabinoid sensor GRAB_eCB2.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   S383T

   Promoter

   hSyn

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEXtev-HMG54kd

   Plasmid

   #26030

   Depositor

   Johann Deisenhofer

   Article

   Istvan et al EMBO J. 2000 Mar 1. 19(5):819-30.

   Insert

   3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Catalytic domain (amino acids 426-488) Methion…

   Promoter

   Available Since

   Dec. 3, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1-CHRM3-cpHalo∆-mEGFP

   Plasmid

   #194678

   Purpose

   CMV driven expression of the m3 GPCR recorder in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   CHRM3_cpHalo∆
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pWPI-GFP-3xFLAG

   Plasmid

   #201639

   Purpose

   Over-expression of 3xFLAG-tagged GFP

   Depositor

   Vamsi Mootha, Alexis Jourdain

   Article

   Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX6P-1-eIF4E K119A

    Plasmid

    #199440

    Purpose

    Bacterial expression plasmids for production of recombinant GST-eIF4E K119A

    Depositor

    Shin-ichi Hoshino

    Article

    Ogami et al STAR Protoc. 2023 May 26;4(2):102340. do

    Insert

    1977 (EIF4E Human)
    

    Use

    Tags

    GST-fusion protein

    Expression

    Bacterial

    Mutation

    changed lysine 119 to alanine

    Promoter

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAGGS puro human-GFP-RLIM-IRES-mCherry

    Plasmid

    #232243

    Purpose

    Mammalian expression of RLIM-GFP-IRES-mCherry

    Depositor

    Francisco Bustos

    Article

    Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

    Insert

    RLIM-GFP-IRES-mCherry (RLIM Human)
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLR5-CAG-44B12mut23(Ser5ph)-SNAP

    Plasmid

    #174877

    Purpose

    PiggyBac transposon vector for expression of RNAPII Ser5ph mintbody

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    RNAPII Ser5ph mintbody-SNAP
    

    Use

    Piggybac vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG/hArf4(WT)-mCherry

    Plasmid

    #79406

    Purpose

    Expresses C-terminally mCherry-tagged Arf4(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Nakai et al Mol Biol Cell. 2013 Aug;24(16):2570-81.

    Insert

    Arf4 (ARF4 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

14. TRE-UBC-HLA-G SCT-T2A-GFP

    Plasmid

    #205462

    Purpose

    lentiviral plasmid for expression of HLA-G SCT

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    HLA-G SCT (HLA-G Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SLC39A14-GFP

    Plasmid

    #104380

    Purpose

    Expresses SLC39A14/ZIP14 wild-type in mammalian cells

    Depositor

    Somshuvra Mukhopadhyay

    Article

    Liu et al J Biol Chem. 2017 Oct 6;292(40):16605-16

    Insert

    SLC39A14 (SLC39A14 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPV-EF1a-mRxxFP1(AfeI)-iP-A

    Plasmid

    #204625

    Purpose

    Cloning vector for SSA vector (mRxxFP), the target sequence can be inserted by In-Fusion reaction after AfeI digestion

    Depositor

    Akitsu Hotta

    Article

    Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFH2.94_PB-TRE3GS-Gag-MCP-T2A-eGFP-PGK-Tet3G-T2A-Blast

    Plasmid

    #205540

    Purpose

    Express HIV Gag-MCP-T2A-eGFP in doxycycline-inducible manner, and constitutively express Tet3G transactivator and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    Gag-MCP
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    TRE3GS

    Available Since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-GfaABC1D-GRAB_Ado1.0med

    Plasmid

    #153289

    Purpose

    Express the medium-affinity adenosine sensor GRAB_Ado1.0med in astrocytes

    Depositor

    Yulong Li

    Article

    Wu et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Adenosine sensor GRAB_Ado1.0med (medium affinity)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    GfaABC1D

    Available Since

    Aug. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SGLT1(GFP)-MAP17(nb)

    Plasmid

    #216210

    Purpose

    for expression of human GFP-tagged SGLT1-MAP17 nanobody complex in BacMam system

    Depositor

    Lei Chen

    Article

    Cui et al Nat Commun. 2023 May 22;14(1):2920. doi:

    Insert

    SGLT1 GFP fusion and MAP17-nanobody fusion (SLC5A2, PDZK1IP1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PA-Dre

    Plasmid

    #169706

    Purpose

    Photoactivatable Dre recombinase for AAV production and expression in mammalian cells

    Depositor

    Dali Li

    Article

    Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

    Insert

    Dre246-nMag-T2A-pMag-Dre247
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available Since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28a SUMO Nup53xl 1-320

    Plasmid

    #85998

    Purpose

    bacterial expression of his6 and SUMO tagged Xenopus laevis Nup53

    Depositor

    Wolfram Antonin

    Article

    Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

    Insert

    Xenopus laevis Nup53 (nup35.L Frog)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    codon optimzed for bacterial expression

    Promoter

    T7

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEN_TT 3xFLAG-CSE1L

    Plasmid

    #192295

    Purpose

    Gateway entry vector for an inducible 3xFLAG-CSE1L

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    CSE1L (CSE1L Human)
    

    Use

    Gateway entry vector

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Non-specific sgRNA

    Plasmid

    #109432

    Purpose

    MLM3636 backbone containing a gRNA that does not bind to any sequence in the human genome.

    Depositor

    Reuben Harris

    Article

    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

    Insert

    Non-specific gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pINDUCER20-Src(Y527F)

    Plasmid

    #215464

    Purpose

    Dox-inducible lentiviral transfer vector with Src constitutively-active mutant (Y527F)

    Depositor

    Johanna Ivaska

    Article

    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

    Insert

    Src (SRC Chicken)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Y527F (constitutively-active)

    Promoter

    mCMV, Doxycycline-inducible

    Available Since

    May 29, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pSLIK 3xFLAG-KPNA1 zeo

    Plasmid

    #192329

    Purpose

    Lentiviral expression vector for an inducible 3xFLAG-KPNA1

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    KPNA1 (KPNA1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. tdTomato-KIF1A

    Plasmid

    #102850

    Purpose

    Overexpresses KIF1A and cell fill TdTomato

    Depositor

    Erik Dent

    Article

    McVicker et al Nat Commun. 2016 Sep 23;7:12741. doi: 10

    Insert

    KIF1A (Kif1a Rat)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pETSUMO2_Vitiosangium-bGSDM-thrombin

    Plasmid

    #222099

    Purpose

    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Vitiosangium species with a thrombin protease cleavage site for controlled activation.

    Depositor

    Philip Kranzusch

    Article

    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

    Insert

    Vitiosangium-bGSDM-thrombin ORF
    

    Use

    Tags

    6xHis-SUMO2

    Expression

    Bacterial

    Mutation

    N-terminal methionine deleted and replaced by sin…

    Promoter

    T7

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pETSUMO2_Nostoc-bGSDM-WELQ

    Plasmid

    #222100

    Purpose

    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Nostoc species with a WELQut protease cleavage site for controlled activation.

    Depositor

    Philip Kranzusch

    Article

    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

    Insert

    Nostoc-bGSDM-WELQ ORF
    

    Use

    Tags

    6xHis-SUMO2

    Expression

    Bacterial

    Mutation

    N-terminal methionine deleted and replaced by sin…

    Promoter

    T7

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pQCXIP-mCherry-CDMPR

    Plasmid

    #182643

    Purpose

    Mammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), followed by mCherry and murine CDMPR (without its signal peptide)

    Depositor

    Martin Spiess

    Article

    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

    Insert

    CDMPR
    

    Use

    Retroviral

    Tags

    N-terminal ER targeting sequence of hemagglutinin…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLGB36

    Plasmid

    #135621

    Purpose

    Suicide vector for allelic replacement in Bacteroides species including B. fragilis strain 638R, erythromycin selection and aTC-inducible ss-Bfe3 counterselection

    Depositor

    Laurie Comstock

    Article

    Ito et al mBio. 2020 Feb 4;11(1). pii: mBio.03238-

    Insert

    bfe3
    

    Use

    Bacteroides suicide vector with inducible counter…

    Tags

    signal sequence for periplasmic targeting

    Expression

    Mutation

    Promoter

    Bacteroides promoter regualted by TetR transcript…

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCDNA5/FRT_CMV_NES-Caprola_09-mEGFP

    Plasmid

    #194685

    Purpose

    CMV driven expression of the calcium recorder Caprola_09 in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_09-mEGFP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. hAAVS1-gs4_EF1a-Cas9-2A-GFP

    Plasmid

    #118414

    Purpose

    CRISPR knockout, expresses Cas9, and sgRNA targeting AAVS1 locus

    Depositor

    Mark Denham

    Article

    Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

    Insert

    gRNA AAVS1 (AAVS1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMSCV-GSDMD-Myc

    Plasmid

    #201166

    Purpose

    Expression of GSDMD gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

    Insert

    Gasdermin D (Gsdmd Mouse)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    MESV

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCDNA5/FRT_CMV_NES-Caprola_off-mEGFP

    Plasmid

    #194687

    Purpose

    CMV driven expression of the inactive calcium recorder negative control Caprola_off in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_off-mEGFP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pH3mCHSH2

    Plasmid

    #101053

    Purpose

    This is a Cryptococcus neoformans mCherry expression vector with G418 drug selection marker and C. neoformans SH2 flanking sequences for genome integration.

    Depositor

    Jennifer Lodge

    Article

    Upadhya et al Fungal Genet Biol. 2017 Sep 1. pii: S108

    Insert

    Cryptococcus mCherry expression plasmid
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Cryptococcus Histone3 promoter

    Available Since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. GEF-H1 FLARE212.pa

    Plasmid

    #134930

    Purpose

    GEF-H1 biosensor that does not have GEF activity.

    Depositor

    Klaus Hahn

    Article

    Azoitei et al J Cell Biol. 2019 Sep 2;218(9):3077-3097

    Insert

    GEF-H1(1-212)-yPet-4X linker-Cerulean3-GEF-H1(213-end)
    

    Use

    Tags

    None.

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV and T7

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLacO-pEF1α-RFP

    Plasmid

    #179507

    Purpose

    Expresses monomeric RFP in mammalian cells under a EF1α promoter

    Depositor

    Yasushi Hiraoka

    Article

    Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

    Insert

    monomeric RFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. GEF-H1 FLARE191.pa

    Plasmid

    #134931

    Purpose

    GEF-H1 biosensor that has GEF activity.

    Depositor

    Klaus Hahn

    Article

    Azoitei et al J Cell Biol. 2019 Sep 2;218(9):3077-3097

    Insert

    GEF-H1(1-191)-yPet-4X linker-Cerulean3-GEF-H1(192-end)
    

    Use

    Tags

    None.

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV and T7

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. GST-Rab8a-wt

    Plasmid

    #86078

    Purpose

    A bacterial expression plasmid encoding Rab8a wild type with N-terminus GST.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    hRab8A (RAB8A Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMCB619

    Plasmid

    #171011

    Purpose

    CRISPR sgRNA vector (puro-BFP with BstXI/BlpI digest sites).

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    U6:sgRNA_Puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pcpBpaRS_v2

    Plasmid

    #196485

    Purpose

    To express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.

    Depositor

    Kensaku Sakamoto

    Article

    Hino et al Methods Mol Biol. 2012;794:215-28. doi:

    Insert

    benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40(synthetase), Pol III tRNA promoter (tRNA), C…

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pBa.GFP-KIF5B

    Plasmid

    #134625

    Purpose

    Mammalian expression of mouse KIF5B 376-963

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF5B (Kif5b Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 377 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available Since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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43. pQCXIP-EGFP-CDMPR

    Plasmid

    #182642

    Purpose

    Mammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), followed by EGFP and murine CDMPR (without its signal peptide)

    Depositor

    Martin Spiess

    Article

    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

    Insert

    CDMPR
    

    Use

    Retroviral

    Tags

    EGFP and N-terminal ER targeting sequence of hema…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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44. pAAV-hGfap-R-CaMP1.07-WPRE-SV40

    Plasmid

    #164140

    Purpose

    AAV vector to express the red Ca2+ indicator R-CaMP1.07 in astrocytes.

    Depositor

    Hajime Hirase, Hirokazu Hirai

    Article

    Oe et al Nat Commun. 2020 Jan 24;11(1):471. doi:

    Insert

    R-CaMP1.07
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hGFAP

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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45. pcDNA3.1-ROR2-V5/HIS

    Plasmid

    #205608

    Purpose

    Expression of human ROR2 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    ROR2 receptor tyrosine kinase (ROR2 Human)
    

    Use

    Tags

    V5-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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46. pCCL-FOXJ1Promoter-FLuc-CMV-RLuc-dsRed2

    Plasmid

    #215328

    Purpose

    Lentiviral mammalian vector with firefly luciferase reporter for FOXJ1 expression; renilla luciferase and dsRed2 reporter under CMV promoter.

    Depositor

    Rob Hynds

    Article

    Orr et al Am J Physiol Lung Cell Mol Physiol. 2024

    Insert

    FOXJ1 Promoter
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    FOXJ1

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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47. PB-TAC-OK+9MS-Cas9

    Plasmid

    #120354

    Purpose

    piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Insert

    OK+9MS cassette
    

    Use

    CRISPR; Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Klf4 [1-483]

    Promoter

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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48. GFP-p38beta

    Plasmid

    #86833

    Purpose

    mammalian expression of GFP-p38beta

    Depositor

    Rony Seger

    Article

    Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

    Insert

    p38beta (MAPK11 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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49. pJJR83

    Plasmid

    #75028

    Purpose

    mCherry^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Mike Boxem

    Article

    Ramalho et al Development. 2020 Jul 22;147(14):dev1880

    Insert

    mCherry^SEC^3xFlag
    

    Use

    CRISPR

    Tags

    3xFLAG and C. elegans codon optimized mCherry

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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50. pLYS1-MICU1-Flag

    Plasmid

    #50058

    Purpose

    Expression of human MICU1 with Flag tag in mammalian cells

    Depositor

    Vamsi Mootha

    Article

    Sancak et al Science. 2013 Nov 14.

    Insert

    MICU1 (isoform 2) (MICU1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    *

    Promoter

    Available Since

    May 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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