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pSG5-BZLF1
Plasmid#72637PurposeMammalian expression vector for Epstein-Barr virus Zta immediate-early transcriptional regulatorDepositorInsertBZLF1
UseTagsExpressionMammalianMutationQ163RPromoterAvailable sinceFeb. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-SV40NLSf-GFP-3xmiR122-WPRE-HGHpA
Plasmid#183775PurposeAAV genome with a CAG driven eGFP reporter with mR122 target sequence repeats to reduce transgene expression in hepatocytes.DepositorInsertNLS-GFP
UseAAVTagsExpressionMutationNAPromoterCAGAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FLAG-REL
Plasmid#27253DepositorInsertREL (REL Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GfaABC1D-GRAB_ATP1.0
Plasmid#167579PurposeExpress the ATP sensor GRAB_ATP1.0 in astrocytesDepositorInsertATP sensor GRAB_ATP1.0 (P2RY1 Human)
UseAAVTagsExpressionMutationPromoterGfaABC1DAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNTI934
Plasmid#216637PurposeCal-ID-PM (V5) Plasma membrane-localized Cal-ID, mammalian expression and lentivirus productionDepositorInsertCal-ID-PM
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET32-MCM2/7
Plasmid#116949PurposeExpresses human MCM2 and human MCM7DepositorUseTagsThioredoxin, His6ExpressionBacterialMutationPromoterAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pInt-ts
Plasmid#66076PurposeThe pInt-ts plasmid [Haldimann2001] is the lambda helper plasmid necessary for the genome integration of the burden monitor lambda versionDepositorInsertlambda integrase
UseTagsExpressionMutationPromoterAvailable sinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
EGFP-OSBP-PH
Plasmid#49571Purposeexpresses the plekstrin homology domain of OSBP in mammalian cellsDepositorInsertOSBP-PH (AA 91-179) (OSBP Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MH6-RspCas13d
Plasmid#108305PurposeExpresses E. coli codon optimized RspCas13d in the pET28a backbone.DepositorInsertRspCas13d
UseCRISPRTagsmH6 tagExpressionBacterialMutationPromoterlacAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-VAPA(1-242)
Plasmid#18874DepositorInsertVAP-A (Vapa Rat)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
CMV-dCasRx-ccdB
Plasmid#221003PurposeDestination vector for Gateway cloning of ORFs into the C-terminus of dCasRx. CMV promoter. Transient expression.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
nAChR beta2 WT
Plasmid#24272DepositorInsertnAChR beta2 WT (Chrnb2 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJMP3
Plasmid#79875PurposeBacillus subtilis sgRNA expression vector; integrates into thrCDepositorInsertsgRNA RR1
UseCRISPRTagsExpressionBacterialMutationPromotervegAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
LRG2.1
Plasmid#108098PurposeLentiviral expression plasmid of sgRNA with GFPDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoter for sgRNA expression and EFS promoter…Available sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFlag-CMV1-hMD2
Plasmid#13028DepositorInsertMD2 (LY96 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
BiP 2: C. elegans BiP (hsp-4)-GFP
Plasmid#21896DepositorInsertBiP (hsp-4 Nematode)
UseTagsGFPExpressionWormMutationFusion between the GFP and hsp-4 takes place at A…PromoterAvailable sinceSept. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-DNMT3A-PuroR (ANV)
Plasmid#71684PurposeExpression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR; cloning backbone for sgRNADepositorInsertS. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Human, S. pyogenes, Synthetic)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-PuroRExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9; E756A inactiva…PromoterCBhAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGOAL19
Plasmid#20190DepositorTypeEmpty backboneUseMarker cassetteTagsExpressionMutationPromoterAvailable sinceMay 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCas3cRh
Plasmid#133773PurposeExpresses all necessary components of Type I-C CRISPR-Cas systemDepositorUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
nAChR alpha4 WT
Plasmid#24271DepositorInsertnAChR alpha4 WT (Chrna4 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDG458
Plasmid#100900PurposeSpCas9 with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.DepositorInsertshumanized CRISPR associated protein 9
U6-gRNA scaffold 1
U6-gRNA scaffold 2
UseCRISPR and Mouse TargetingTags3xFLAGExpressionMammalianMutationPromoterCBh and U6Available sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_RELB_WT
Plasmid#82232PurposeGateway Donor vector containing RELB , part of the Target Accelerator Plasmid Collection.DepositorInsertRELB (RELB Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
PB-PGK-teD-Cit
Plasmid#116041PurposeReporter plasmid for measuring protease activityDepositorInsertteD-Cit
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro-GFP-wt-lamin A
Plasmid#17662DepositorInsertGFP-lamin A (LMNA Human)
UseRetroviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pUC-hrDNA-12.0
Plasmid#206320PurposeA plasmid containing a 12.0 kb EcoRI fragment that corresponds to sequences between 30.5 and 42.5 kb of the human rDNA repeat (GenBank: U13369)DepositorInsertIntergenic spacer of human rDNA repeat
UseCloningTagsExpressionMutationPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRDA_221
Plasmid#169142PurposeConstitutive expression of EGFP and sgRNAs targeted to EGFP for measurement of activity of AsCas12a\EnAsCas12a.DepositorInsertEGFP , EGFP sgRNA
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXM1
Plasmid#153136PurposeExpression of Flag tagged human FOXM1 in mammalian cellsDepositorInserthuman FOXM1 (FOXM1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6-Sp-pegRNA-HEK3_CTT_ins
Plasmid#132778PurposeS. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editingDepositorInsertHEK3_CTT_ins pegRNA
UseTagsExpressionMammalianMutationSee manuscriptPromoterU6Available sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
lenti-mTagBFP2
Plasmid#216126PurposeLentiviral vector for expressing mTagBFP2.DepositorInsertEF1a-mTagBFP2-WPRE
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pICH47761
Plasmid#48003DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceDec. 3, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pM355
Plasmid#173175Purpose(Empty Backbone) Expresses gRNA-12xMBS from hU6 promoterDepositorInsertgRNA-12xMBS backbone
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLPCX cyto Grx1-roGFP2
Plasmid#64975PurposeMammalian expression of cytosolic Grx1-roGFP2 (retroviral vector)DepositorInsertGlutaredoxin-1 (GLRX Human)
UseRetroviralTagsroGFP2ExpressionMammalianMutationPromoterAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
1236 pcDNA3 Myr HA Akt3
Plasmid#9017DepositorInsertAkt3 (AKT3 Human)
UseTagsHA and MyrExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2005AvailabilityAcademic Institutions and Nonprofits only -
AAV-EFS-SauriABE8e-bGH-U6-sgRNA-BsmBI
Plasmid#189925PurposeAAV genome encoding SauriABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installationDepositorInsertSauriABE8e
UseAAVTagsExpressionMutationSauriCas9 D15APromoterEFSAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EF1alpha-dCas9-p300_Blast
Plasmid#192653Purpose3rd generation lenti vector encoding dCas9-p300 with 2A Blast resistance markerDepositorInsertdCas9-p300
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A; H840A in Cas9PromoterEF1aAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF1538
Plasmid#143823PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZBTB46 (ZBTB46 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_SFFv_Her2-BFP_RHL027
Plasmid#164823PurposeLentiviral vector for constitutive expression of HER2+TM(23-675)-BFPDepositorInsertExtracellular and transmembrane region of ERBB2 (ERBB2 Human)
UseLentiviralTagstagBFPExpressionMutationPromoterSFFVAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-TRAF1
Plasmid#111199Purposefluorescent fusion proteinDepositorInsertTRAF1 (TRAF1 Human)
UseTagsEGFP-FLAGExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
VCP(wt)-EGFP
Plasmid#23971DepositorInsertValosin containing protein (VCP Human)
UseTags6xHis, EGFP, and HAExpressionMammalianMutationnonePromoterAvailable sinceFeb. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
pNTI825
Plasmid#216633PurposeCal-ID (V5) for AAV productionDepositorInsertCal-ID
UseAAVTagsV5ExpressionMammalianMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET30a-His6-LOXCAT
Plasmid#140084PurposeFusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2.DepositorInsertA fusion of lactate oxidase from A. viridans and catalase from E. coli
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSC-humanADAR1
Plasmid#198235PurposeExpresses human ADAR1 in yeastDepositorInserthuman ADAR1 (ADAR Human)
UseTagsHis10_TEVExpressionYeastMutationPromoterGAL1Available sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MAP2K1
Plasmid#23406DepositorInsertMAP2K1 (MAP2K1 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-mCherryP2ACre
Plasmid#161773PurposeFlp-Dependent mCherry and Cre expressionDepositorInsertmCherry-P2A-Cre
UseAAVTagsExpressionMammalianMutationPromoterEf1aAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pCMV-ABE7.10
Plasmid#102919Purposeexpresses ABE7.10 in mammalian cellsDepositorInsertABE7.10
UseTagsNLSExpressionMammalianMutationsee manuscriptPromoterCMVAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmCherry C1 Omp25
Plasmid#157758PurposeExpression of mcherry-labelled mitochondrial outer membrane protein 25 in cellsDepositorInsertSYNJ2bp (SYNJ2BP Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
FURIN-bio-His
Plasmid#51755PurposeExpresses full-length Furin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertFURIN (FURIN Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAJE3-E7
Plasmid#214359PurposeExpresses the 2nd-generation "E7" Tet2 Methanocaldococcus jannaschii (Mj) aminoacyl tRNA synthetase/tRNA pair for encoding Tet2 noncanonical amino acids into TAG sites of proteinsDepositorInserts2nd generation Tet2 "E7" aminoacyl tRNA synthetase
Lpp promoted M. jannaschii tRNA
Aminoglycoside-3''-adenyltransferase (Spectinomycin/streptomycin Resistance Gene)
Orthogonal "D4II" ColE1 origin
UseTagsExpressionBacterialMutationPromoterAmpR and lppAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only
