161,641 results

1. mCh-KIF5B*-strep

   Plasmid

   #120164

   Purpose

   Expresses a chimera of mCherry (fluorescent tag), a KIF5B-based anterograde motor and streptavidin

   Depositor

   Juan Bonifacino

   Article

   Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

   Insert

   kinesin family member 5B (Kif5b Mouse)
   

   Use

   Tags

   Streptavidin and mCherry

   Expression

   Mammalian

   Mutation

   Truncated KIF5B: 1-555 aa

   Promoter

   CMV

   Available since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMSCV mKeima-LC3

   Plasmid

   #189006

   Purpose

   Fluorescent reporter for autophagy

   Depositor

   Atsushi Hoshino

   Article

   Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

   Insert

   mKeima, LC3 (Map1lc3b Rat)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV_CMV::V5-EGFR

   Plasmid

   #199717

   Purpose

   Expresses V5-tagged EGFR in mammalian cells.

   Depositor

   Alice Ting

   Article

   Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

   Insert

   V5-EGFR
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p23-NES-RfxCas13d-msfGFP-NES-Flag

   Plasmid

   #165076

   Purpose

   overexpression of RfxCas13d in human cells

   Depositor

   Ling-Ling Chen

   Article

   Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

   Insert

   RfxCas13d
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMXs-Oct3/4

   Plasmid

   #13366

   Purpose

   Retroviral expression of mouse Oct3/4

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

   Insert

   POU domain, class 5, transcription factor 1 (Pou5f1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTRIP-SFFV-mtagBFP-2A RIGI WT

   Plasmid

   #167289

   Purpose

   Lentiviral expression construct encoding a TagBFP in frame with a self-cleaving wild-type RLR sensor RIG-I

   Depositor

   Agnel Sfeir

   Article

   Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

   Insert

   DDX58 (RIGI Human)
   

   Use

   Lentiviral

   Tags

   tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSL-MS2-12X

   Plasmid

   #27119

   Depositor

   Robert Singer

   Article

   Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

   Insert

   MS2 binding sites (12)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTREtight2

   Plasmid

   #19407

   Depositor

   Markus Ralser

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT

   Plasmid

   #204694

   Purpose

   This plasmid allows for inducible expression of full-length BRD4-NUT fusion tagged with mNeonGreen, in mammalian cells.

   Depositor

   Michael Rosen

   Article

   Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

   Insert

   BRD4-NUT (BRD4, NUTM1 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   direct fusion of BRD4 and NUT

   Promoter

   CMV

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMSCV-IRES-GFP-human-Caspase-1

    Plasmid

    #183359

    Purpose

    Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-1 (CASP1 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    MESV

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AAVS1 CAG rtTA3 TauWT 2N4R-EGFP

    Plasmid

    #132389

    Purpose

    Exchange cassette containing inducible TauWT 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site

    Depositor

    Gerold Schmitt-Ulms

    Article

    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

    Insert

    human TauWT 2N4R-EGFP (MAPT Synthetic, Human)
    

    Use

    CRISPR and Cre/Lox

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    TREtight

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPPAT-EGFP

    Plasmid

    #99105

    Purpose

    Expresses human PPAT-EGFP fusion in mammalian cells

    Depositor

    Stephen Benkovic

    Article

    An et al Science. 2008 Apr 4;320(5872):103-6. doi

    Insert

    PPAT (PPAT Human)
    

    Use

    Tags

    EGFP (enhanced green fluorescent protein)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-Syn-Chronos-tdTomato

    Plasmid

    #62726

    Purpose

    AAV-mediated expression of Chronos-tdTomato under the Syn promoter. Using bGHpA signal. tdTomato has codons varied between the first and second tandem repeats to reduce recombination.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Has Service

    AAV8

    Insert

    Chronos-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin promoter

    Available since

    April 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAG_smFP HA

    Plasmid

    #59759

    Purpose

    encodes multiple copies of an epitope tag HA

    Depositor

    Loren Looger

    Article

    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

    Insert

    smFP _HA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-CAG-Cre-3xmiR122-WPRE-HGHpA

    Plasmid

    #183776

    Purpose

    AAV genome with a CAG driven Cre recombinase with mR122 target sequence repeats to reduce transgene expression in hepatocytes.

    Depositor

    Ben Deverman

    Article

    Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-40

    Insert

    Cre
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    CAG

    Available since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG-MCS-TurboID-3xFLAG

    Plasmid

    #186814

    Purpose

    CAG promoter-driven expression of BioID2-fused protein (C terminus)

    Depositor

    Masahito Ikawa

    Article

    Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

    Type

    Empty backbone

    Use

    Tags

    TurboID-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-hSyn-fDIO-mCherry-WPREpA

    Plasmid

    #177328

    Purpose

    Control vector for Flp-dependent DREADD constructs

    Depositor

    Ulrik Gether

    Insert

    mCherry
    

    Use

    AAV; Flp-frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human Synapsin-1

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pH6HTC_CCT2-Halo

    Plasmid

    #175337

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    CCT2 (CCT2 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TFORF1337

    Plasmid

    #144171

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZBTB7A (ZBTB7A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCP60 RS-GFP

    Plasmid

    #122173

    Purpose

    "Binary vector for Agrobacterium tumefacience-mediated transformation of plant cells resulting in constitutive expression of cytoplasmatically localized RS-GFP"

    Depositor

    Lukas Fischer

    Article

    Nocarova et al BMC Plant Biol. 2009 Apr 22;9:44. doi: 1

    Insert

    RS-GFP
    

    Use

    Binary vector for escherichia coli and agrobacter…

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pFA6a-GFP(S65T)-kanMX6

    Plasmid

    #39292

    Depositor

    John Pringle, Jurg Bahler

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    KanR
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    Promoter

    Available since

    Aug. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. L_NLS 41kDa

    Plasmid

    #201342

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    (NLS)SV40A4-EGFP-2PrA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. mCherry-MyosinIIB-N-18

    Plasmid

    #55107

    Purpose

    Localization: Cytoskeleton, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    MyosinIIB (MYH10 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

24. pRsetB-PercevalHR

    Plasmid

    #49081

    Purpose

    Bacterial expression vector of N-terminal His-tagged PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

    Depositor

    Gary Yellen

    Article

    Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

    Insert

    PercevalHR
    

    Use

    Tags

    7xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDisplay-GRAB_OT1.0

    Plasmid

    #185384

    Purpose

    Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    GPCR activation based oxytocin sensor GRAB_OT1.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-pTH-iCre-WPREpA

    Plasmid

    #213130

    Purpose

    Truncated rat TH promoter expressing iCre

    Depositor

    Ulrik Gether

    Article

    Lycas et al Cell Rep. 2022 Sep 27;40(13):111431. doi

    Insert

    iCre
    

    Use

    AAV and Mouse Targeting

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    Truncated TH promoter from rat (Rattus norvegicus…

    Available since

    Feb. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pNLF1-N_CCT5:1-541

    Plasmid

    #210978

    Purpose

    Mammalian expression of full-length human CCT5. N-terminal NanoLuc luciferase tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    CCT5:M1-E541 (CCT5 Human)
    

    Use

    Tags

    NanoLuc luciferase

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLentiRNAGuide_004-hU6-RfxCas13d-DR1-BsmBI-EFS-EGFP-P2A-Puro-WPRE

    Plasmid

    #223175

    Purpose

    For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

    Insert

    hU6_Rfxcas13d-DR1_BsmBI
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    DR1 A>T

    Promoter

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. LentiCRISPRv2-Opti

    Plasmid

    #163126

    Purpose

    LentiCRISPRv2 variant with modified sgRNA scaffold from Chen et al. 2013 Cell.

    Depositor

    David Sabatini

    Article

    Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSLHDR01_mCherry_tCD19

    Plasmid

    #123661

    Purpose

    Construction of 1st donor for scarless genome editing

    Depositor

    Matthew Porteus

    Article

    Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

    Type

    Empty backbone

    Use

    Mouse Targeting; Human targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pRGD-GmR

    Plasmid

    #74106

    Purpose

    Plasmid containing gentamicin resistance gene cassette flanked by multiple restriction sites

    Depositor

    Hans-Martin Fischer

    Article

    Ledermann et al Appl Environ Microbiol. 2016 Feb 26. pii

    Insert

    aacC1
    

    Use

    Bacterial vector based on pmb1 replicon

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pWZL Neo Myr Flag PDK1

    Plasmid

    #20564

    Depositor

    William Hahn, Jean Zhao

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    PDK1 (PDK1 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pVC-FUS-R521C

    Plasmid

    #175067

    Purpose

    expresses mVenus and mCherry tagged to mutant of the FUS (R521C) protein in mammalian cells

    Depositor

    Arnold Boersma

    Article

    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

    Insert

    FUS glycine rich protein (FUS Human)
    

    Use

    Tags

    HA and mVenus + mCherry

    Expression

    Mammalian

    Mutation

    changed Arginin 521 to Cystein; mVenus: C-Termina…

    Promoter

    CMV Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSEVA63-GG

    Plasmid

    #208015

    Purpose

    for cloning homology arms, with GFP as counter selection marker

    Depositor

    Pablo Ivan Nikel

    Article

    Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

    Insert

    msfGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    14G

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMRX-IZ-ANXA5-mRuby3

    Plasmid

    #184891

    Purpose

    Stable expression of ANXA5-mRuby3 (Annexin A5-mRuby3) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

    Insert

    ANXA5 (ANXA5 Human)
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. 5_T7-GAP43-mScarlet3

    Plasmid

    #225932

    Purpose

    In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the GAP43 membrane localisation signal

    Depositor

    Michalis Averof

    Article

    Karapidaki et al bioRxiv 2024.11.12.623055

    Insert

    GAP43-mScarlet3
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pXR004: CasRx pre-gRNA cloning backbone

    Plasmid

    #109054

    Purpose

    hU6-driven expression of guide RNAs compatible with CasRx. Contains BbsI sites for guide cloning flanked by 5' and 3' full-length DRs

    Depositor

    Patrick Hsu

    Article

    Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. MSCV-N EBNA1

    Plasmid

    #37954

    Depositor

    Karl Munger

    Article

    Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

    Insert

    EBNA1 (EBNA-1 H. Herpesvirus 4 (EBV))
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    PKG

    Available since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. qTAG-C-moxGFP-Zeo

    Plasmid

    #207732

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. pEGFP-C1-PRKAA1

    Plasmid

    #30305

    Depositor

    Jay Brenman

    Article

    Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

    Insert

    AMP-activated Protein Kinase α1 (Prkaa1 Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCAGGS-FuG-B2

    Plasmid

    #67513

    Purpose

    This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. You can make type B2 envelope coating virus particle with HiRet by using this plasmid.

    Depositor

    Kazuto Kobayashi

    Article

    Kato et al J Neurosci. 2011 Nov 23;31(47):17169-79.

    Insert

    Fusion Glycoprotein type B2
    

    Use

    Lentiviral

    Tags

    Fusion protein of Vesicular stomatitis Indiana vi…

    Expression

    Mutation

    Promoter

    CAGGS

    Available since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pGL198

    Plasmid

    #180472

    Purpose

    For expression of Cas9 and gRNAs, multiple gRNAs cloning vector

    Depositor

    Peggy Lemaux

    Article

    Aregawi et al Plant Biotechnol J. 2021 Nov 26. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    ZmCas9

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pBAD/His-B-iRFP

    Plasmid

    #31855

    Purpose

    a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

    Depositor

    Vladislav Verkhusha

    Article

    Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

    Insert

    iRFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S13L, A92T, V104I, V114I, E161K, Y193K, F198Y, D2…

    Promoter

    araBAD

    Available since

    Sept. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pJAT17

    Plasmid

    #204298

    Purpose

    For introducing Cas9 under D. melanogaster nanos promoter. Markers with EYFP expressed in eyes.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    3XP3-EYFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    3XP3

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. TFORF2258

    Plasmid

    #142927

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SOX11 (SOX11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. MBP-FUS_FL_WT

    Plasmid

    #98651

    Purpose

    Expresses MBP-tagged full length FUS

    Depositor

    Nicolas Fawzi

    Article

    Burke et al Mol Cell. 2015 Oct 15;60(2):231-41. doi:

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pEGFP SNAP23

    Plasmid

    #101914

    Purpose

    Expresses SNAP23 in mammalian cells

    Depositor

    Thierry Galli

    Article

    Kuster et al J Biol Chem. 2015 Nov 20;290(47):28056-6

    Insert

    SNAP 23 (SNAP23 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. TFORF2018

    Plasmid

    #142311

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PITX3 (PITX3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pHIV-EGFP

    Plasmid

    #21373

    Purpose

    self-inactivating lentiviral plasmid for co-expression of your gene of interest and EGFP

    Depositor

    Zena Werb, Bryan Welm

    Article

    Welm et al Cell Stem Cell. 2008 Jan 10. 2(1):90-102

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pOpen-SP6rpol

    Plasmid

    #165516

    Purpose

    DNA-dependent RNA polymerase derived from SP6 bacteriophage that catalyzes the transcription of viral genes.

    Depositor

    Jennifer Molloy, FreeGenes Project, Drew Endy

    Insert

    SP6 RNA Polymerase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 29, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge