We narrowed to 14,204 results for: RING;
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Plasmid#83924PurposeCo-expresses cytoplasmic copies of the Skp protein folding chaperone and wild type human lysozyme..DepositorAvailable SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only
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CROP_C9_Puro_ESR1
Plasmid#183288PurposeAll-in-One CRISPRko system with a guide RNA that targets ESR1 geneDepositorInsertESR1
UseLentiviralAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0301
Plasmid#91005PurposeModule A, Promoter: 35S, Gene: AtCas9_H840A nickase, Terminator: HSPDepositorInsertAtCas9_H840A nickase
UseCRISPRMutationH840APromoter35SAvailable SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-005
Plasmid#78692PurposeA weak promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a strong RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
UseSynthetic BiologyAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPPC020.306
Plasmid#171148PurposeExpression of J3-BBa_J23117-mRFP and J306 scRNA on pBBR1-GmR plasmidDepositorInsertsJ306 scRNA
J3-BBa_J23117-mRFP
UseCRISPRExpressionBacterialPromoterBBa_J23119 and J3-BBa_J23117Available SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC0.017
Plasmid#119621PurposeLevel 0 Part. CDSDepositorInsertdCas9
UseSynthetic BiologyMutationbp 331 C to A , 1237 A to C, 1284 C to T, 2121 C …Available SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
S3_002_026_096
Plasmid#134403PurposepYTK096-pRPL18B_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
NLS_FdeR-N
TagsNone and noneExpressionYeastMutationNone and nonePromoterpGPM1 and pRPL18BAvailable SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
FAK
Plasmid#186141PurposeFor expresseion of recombinant FAK using baccolovirus in SF9 cellsDepositorAvailable SinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSB1A2QS
Plasmid#138268PurposeMINPA Gene circuit expression in E. coli. GFP serves as a reporter for Plux/tet promoter activity. mCherry serves as reporter for Para/lac promoter activityDepositorInsertsAraC-TetR operon
LuxR-LacI operon
mCherry
GFP
UseSynthetic BiologyExpressionBacterialPromoterPara/lac and Plux/tetAvailable SinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pES002 (pTet-qacR wt)
Plasmid#69031Purposewild-type qacR downstream of a Tet promoterDepositorAvailable SinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLPAR1_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
Plasmid#101115PurposeEncodes dCas9(C)-VP64 with SAM components fused to LPAR1DepositorInsertLPAR1-dCas9(C)-VP64
UseCRISPR and Synthetic BiologyTagsFlag and HAExpressionMammalianAvailable SinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-LDR
Plasmid#173857PurposeEncoding a part of the rapamycin-induced dimerization system.DepositorInserta Lyn11-targeted FRB (LDR)
ExpressionMammalianAvailable SinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMK110
Plasmid#99535Purposecontains bgl operon sequence for E. coli in vitro transcription templateDepositorInsertE. coli bgl antisense region containing bglG
TagsNonePromoterlambda Pr promoter used for in vitro transcriptio…Available SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 EB1 shRNA #1
Plasmid#37928DepositorAvailable SinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAP1837
Plasmid#137086PurposePlasmid for PCR-generated donor DNA for rapid tagging of proteins with mCherryDepositorInsertmCherry
UsePcr template for donor dnaExpressionBacterialAvailable SinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAP3041
Plasmid#137083PurposePlasmid for PCR-generated donor DNA for rapid tagging of proteins with GFP11-3xFlag-tagRFPDepositorInsertGFP11::3xFlag::tagRFP
UsePcr template for donor dnaExpressionBacterialAvailable SinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI4A5C6G7G-J23106-geneIII
Plasmid#80909PurposePhagemid (encodes cI4A5C6G7G and M13 gene III)DepositorInsertsM13 gene III
cI4A5C6G7G
ExpressionBacterialMutation238G to 238D (please see depositor comment below)PromoterBBa_J23106 and rpoNAvailable SinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZF-BCB-GCN
Plasmid#127476Purposecoding sequence for synthetic zinc finger ZF-BCB-GCNDepositorInsertZF-BCB synthetic transcription factor fused with GCN4 leucine zipper
UseCell-free transcription–translationTagsGCN4 leucine zipperPromoterT7Available SinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMP315
Plasmid#134994PurposeKRAB-m6A Reader. Repress transcription near Gm6ATC sites. pUBC-KRAB-NLS-DpnI(aa146-254)DepositorInsertpUBC-KRAB-NLS-DpnI(aa146-254)
TagsDpnI(aa146-254) and KRAB domainExpressionMammalianAvailable SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDD2703
Plasmid#175079PurposeMammalian expression of Flag-tagged KSHV ORF45 E223A-S226A mutantDepositorInsertKSHV ORF45 (ORF45 Kaposi's sarcoma-associated herpesvirus 8 )
ExpressionMammalianMutationE223A, S226AAvailable SinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A3910
Plasmid#91054PurposeModule A, Promoter: ZmUbi, Gene: dTaCas9-VPR, Terminator: HSPDepositorInsertdTaCas9-VPR
UseCRISPRPromoterZmUbiAvailable SinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCXCR4_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
Plasmid#101113PurposeEncodes dCas9(C)-VP64 with SAM components fused to CXCR4DepositorInsertCXCR4-dCas9(C)-VP64
UseCRISPR and Synthetic BiologyTagsFlag and HAExpressionMammalianAvailable SinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCXCR4_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE
Plasmid#101114PurposeEncodes dCas9(N) fused to CXCR4DepositorInsertCXCR4-dCas9(C)-VP64
UseCRISPR and Synthetic BiologyTagsFlag and HAExpressionMammalianAvailable SinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0201
Plasmid#91002PurposeModule A, Promoter: 35S, Gene: AtCas9_D10A nickase, Terminator: HSPDepositorInsertAtCas9_D10A nickase
UseCRISPRMutationD10APromoter35SAvailable SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Aa3.8.4
Plasmid#136375PurposeGateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 with MS2 at the third stem loop is usedDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-mCherry-Emi1_control UTRs
Plasmid#67925PurposeExpresses mCherry-Emi1 with control UTRs in mammalian cellsDepositorAvailable SinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZF-BCB-PDZ
Plasmid#127478Purposecoding sequence for synthetic zinc finger ZF-BCB-PDZDepositorInsertZF-BCB synthetic transcription factor fused with the alpha 1-syntrophin PDZ domain
UseCell-free transcription–translationTagsalpha 1-syntrophin PDZ domainPromoterT7Available SinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
4xHRE_MLP-sfGFP-CMV_dsRed Exp
Plasmid#117403PurposesfGFP expressed from hypoxia-inducible MLP promoter and dsRed expressed from consitutive CMV promoterDepositorInsertSuperFolder GFP
ExpressionMammalianAvailable SinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSR35
Plasmid#69256Purposephsp16.41::CRE for integration on ttTi14024, Chr XDepositorInsertCre recombinase
UseCre/Lox; MossciExpressionWormMutationcodon-optimzed index 1.0Promoterhsp16.41Available SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB6681
Plasmid#106157PurposeEasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6637, integration into Yarrowia lipolytica chromosomal location IntE_3, USER site for cloning, amp resistanceDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCA0.001
Plasmid#119557PurposeLevel 0 acceptor vector for promoters truncated to transcriptional start site with lacZ compatible with blue/white screeningDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
JBEI-6823
Plasmid#84986PurposeFor expression of MevTaco-PMKco-MKco(aka 2B) under lacUV5 promoter for isopentenol productionDepositorInsertMevTaco-PMKco-Mkco
ExpressionBacterialPromoterLacUV5Available SinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
JBEI-6833
Plasmid#84983PurposeExpression of NudB (E. coli) and PMD (S. cerevisiae) are under control of Trc promoter for isopentenol production.DepositorInsertNudB-PMD
ExpressionBacterialPromotertrc promoterAvailable SinceDec. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQCi
Plasmid#154345PurposeSelf-Cutting and Integrating CRISPR backbone (px458 derivative with Cas9-intron, sgRNA, and bait integration sites)DepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AaCas12b(D977A)-2AeGFP
Plasmid#121962PurposeMammalian expression, Transcription regulation, deactivated AaCas12bDepositorInsertCatalytically deactivated AaCas12b
Tags3xHA tagExpressionMammalianMutationD977AAvailable SinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Hmga2 m2
Plasmid#14790DepositorInsertHmga2 m2 (Hmga2 Mouse)
ExpressionMammalianMutationHmga2 m2 mutant (see article and author's ma…Available SinceApril 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
ShaCas9
Plasmid#163794PurposeExpresses ShaCas9, and cloning backbone for sgRNADepositorTypeEmpty backboneUseAAV and CRISPRExpressionMammalianAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
PI(LM)-Rac1(WT)dc1.FLARE
Plasmid#87590PurposePI(LM): Photo-inhibitable/lit mutant I510E/I539E dc1.FLARE: built based on GTPase FLARE dual chain sensorDepositorInsertsLOV2
PBD
Rac1
TagsYPet and dTurqExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0040
Plasmid#117607PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_NbPDS(pEPOR1CB0058)DepositorInsert[35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
ExpressionPlantAvailable SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNB774
Plasmid#60114PurposeGreen beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA)DepositorInsertGrLuc
UseUnspecifiedTagsClpXP degron and Nuclear localization signal (NLS…Available SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJZ001
Plasmid#120230PurposeContains intB13 integrase casette for knock-in to pCAP-BAC backboneDepositorInsertintB13 integrase casette
ExpressionBacterialMutationbla-g717a (amp resistance gene)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR28
Plasmid#69253Purposephlh-8::CRE for integration on ttTi14024, Chr XDepositorInsertCre recombinase
UseCre/Lox; MossciExpressionWormMutationcodon-optimzed index 1.0Promoterhlh-8Available SinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-014
Plasmid#78702PurposeA weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of GFP under the control of a strong RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP-Terminator
UseSynthetic BiologyAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCas2D
Plasmid#82375PurposedCas9 expressed from aTc-inducible promoter in acsA, D RBS: AGGACGDepositorInsertdCas9
PromoterPEZ3Available SinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJAID5VY
Plasmid#165048PurposeIntegrative expression of CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>CUP1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
ExpressionYeastMutationOsTIR1 is codon-optimised for expression in S. ce…Available SinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_RsLP-LOV_X
Plasmid#131177PurposeExpresses RsLP-LOV (Rhodobacter sphaeroides) followed by restriction sitesDepositorInsertRsLP-LOV
ExpressionMammalianPromoterCMVAvailable SinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX28
Plasmid#46850PurposeTOP RFP op. PGK-puro indicator plasmid to assay TOP IMCE efficiencyDepositorInsertattB, RFP and PGK-puro
ExpressionMammalianPromoterPGKAvailable SinceAug. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBW216J106-Amp32C
Plasmid#78651PurposepSB3K3 carrying J106-30hrpR-32hrpS-t-hrpL-30gfp-tTDepositorInsertJ106-30hrpR-32hrpS-t-hrpL-30gfp-t
UseSynthetic BiologyPromotersee insertAvailable SinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJL1-PorA-4xDQNAT
Plasmid#128392PurposeIn vitro expression of N. meningitidis PorA with C terminal 4xDQNAT glycosylation sitesDepositorInsertPorA
Tags4xDQNAT glycosylation tag and 6xHis tagExpressionBacterialPromoterT7Available SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL1-TTc-4xDQNAT
Plasmid#128393PurposeIn vitro expression of tetanus toxin fragment C with C terminal 4xDQNAT glycosylation sitesDepositorInsertTetanus toxin fragment C domain
Tags4xDQNAT glycosylation tag and 6xHis tagExpressionBacterialPromoterT7Available SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only