We narrowed to 18,363 results for: Tec
-
-
-
-
-
-
-
-
-
-
pcDNA3.1-HaloAKAR2.1-CLC
Plasmid#216492PurposeExpression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) targeted to clathrin coated pitsDepositorInsertHaloAKAR2.1-CLC
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
ER-NFAST
Plasmid#233596PurposeExpression of NFAST on the ER membraneDepositorInsertNFAST
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
ER-frNFAST
Plasmid#233599PurposeExpression of frNFAST on the ER membraneDepositorInsertfrNFAST
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
PM-frNFAST
Plasmid#233602PurposeExpression of frNFAST on the PM facing the cytosolDepositorInsertfrNFAST
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
PGK-HA-PGRN
Plasmid#234872PurposeLentiviral plasmid expressing HA-tagged human progranulin.DepositorInsertProgranulin (GRN Human)
UseLentiviralTagsHA tagExpressionMammalianMutationPromoterPGKAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pscAAV.CAG-EGFP-SV40pA-T7-T3-BC(p1-14)
Plasmid#231346PurposeSelf complementary AAV genome with components for tracking self-complementary AAV genomes via AAV-Zombie. Also expresses EGFP from CAG promoter.DepositorInsertT7/T3-BC(p1-14)
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pscAAV.BC(p1-6)-CAG-EGFP-SV40pA-BC(p1-6)
Plasmid#231349PurposeSelf complementary AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from CAG promoter.DepositorInsertBC(p1-10)
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
GB4635
Plasmid#215263PurposeModule for the expression of HISPS under the copper inducible CBS:mDFR promoter and H3H under the red light regulated Etr8:CMVmin promoter and the constitutive expression of LUZ, CPH,EGFP and p19DepositorInsertCBS4x:mDFR:HISPS-Etr4:mDFR:H3HSF-LUZ-CPH-EGFP-p19
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(7CNW)RS
Plasmid#222874PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 7-Cyano-L-Tryptophan (7-CN-Trp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
CAG-mCherry
Plasmid#229135PurposeRetrovirus driving mCherry reporterDepositorInsertmCherry
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ 3xFLAG-FEM1B WT
Plasmid#230993PurposeTransient overexpression of mouse FEM1B in mammalian cellsDepositorInsertFEM1B (Fem1b Mouse)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(5CNW)RS
Plasmid#222872PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 5-Cyano-L-Tryptophan (5-CN-Trp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
RhoA gRNA #1
Plasmid#198718PurposeCas9-mediated knockout of RhoA in mammalian cells #1DepositorInsertRhoA (Rhoa Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
RhoA gRNA #3
Plasmid#198720PurposeCas9-mediated knockout of RhoA in mammalian cells #3DepositorInsertRhoA (Rhoa Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
RhoB gRNA #1
Plasmid#198721PurposeCas9-mediated knockout of RhoB in mammalian cells #1DepositorInsertRhoB (Rhob Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
RhoB gRNA #2
Plasmid#198722PurposeCas9-mediated knockout of RhoB in mammalian cells #2DepositorInsertRhoB (Rhob Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
RhoB gRNA #3
Plasmid#198723PurposeCas9-mediated knockout of RhoB in mammalian cells #3DepositorInsertRhoB (Rhob Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
RhoC gRNA #1
Plasmid#198724PurposeCas9-mediated knockout of RhoC in mammalian cells #1DepositorInsertRhoC (Rhoc Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
RhoC gRNA #2
Plasmid#198725PurposeCas9-mediated knockout of RhoC in mammalian cells #2DepositorInsertRhoC (Rhoc Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
RhoC gRNA #3
Plasmid#198726PurposeCas9-mediated knockout of RhoC in mammalian cells #3DepositorInsertRhoC (Rhoc Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-DXO
Plasmid#177992PurposeFor gateway cloning of DXODepositorInsertDXO (DXO Human)
UseTagsExpressionMutationPromoterAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-UnaG-P2A-FusionRed
Plasmid#191109PurposeMammalian cell line expression plasmids for production of UnaG and FusionRed proteinsDepositorInsertUnaG
UseAAVTagsFusionRedExpressionMammalianMutationSee depositor comments belowPromoterchicken β-actin promoterAvailable sinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-miniCGBE1
Plasmid#198548PurposeExpresses human codon-optimized SpCas9-miniCGBE1 and blasticidin resistance: EFS promoter-SpCas9-miniCGBE1-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-miniCGBE1
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NG-miniCGBE1
Plasmid#198538PurposeExpresses human codon-optimized SpCas9-NG-miniCGBE1 and blasticidin resistance: EFS promoter-SpCas9-NG-miniCGBE1-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NG-miniCGBE1
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NRRH
Plasmid#198540PurposeExpresses human codon-optimized SpCas9-NRRH and blasticidin resistance: EFS promoter-SpCas9-NRRH-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NRRH
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NRCH
Plasmid#198542PurposeExpresses human codon-optimized SpCas9-NRCH and blasticidin resistance: EFS promoter-SpCas9-NRCH-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NRCH
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-PARP11
Plasmid#184469PurposeFor gateway cloning of PARP11DepositorInsertPARP11 (PARP11 Human)
UseTagsExpressionMutationPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-NS3/4A S139A
Plasmid#186885PurposeDoxycycline-dependent expression of catalytically inactive NS3/4A protease gene (S140A) in mammalian cells by retroviral transductionDepositorInsertHCV NS3/4A protease with S139A mutation
UseRetroviralTagsExpressionMutationS139APromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-WHG cGAS-HA
Plasmid#186867PurposeDoxycycline-dependent expression of white-handed gibbon cGAS gene in mammalian cells by retroviral transductionDepositorInsertWhite-handed gibbon cGAS with C-terminal HA tag
UseRetroviralTagsHAExpressionMutationPromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYAMTr2GCsgSc-SeLEU2
Plasmid#224871PurposeDisruption of LEU2 genes in the Saccharomyces sense strict group speciesDepositorUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYFAC-CH2-ubi-Y-pyrG
Plasmid#221131PurposeExpression of heterologous genes under alcohol induction optimised with ubi-Y-pyrG marker. Promoters PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction sites: PacI, NotI, AsiSI, AscI.DepositorTypeEmpty backboneUseSynthetic Biology; Expression in aspergillus and …TagsExpressionMutationPromoterPalcA-T1-PalcS/M-T2-PaldA expression cassette (al…Available sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYFAC-CH2-ubi-M-pyrG
Plasmid#221132PurposeExpression of heterologous genes under alcohol induction optimised with ubi-M-pyrG marker. Promoters PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction sites: PacI, NotI, AsiSI, AscI.DepositorTypeEmpty backboneUseSynthetic Biology; Expression in aspergillus and …TagsExpressionMutationPromoterPalcA-T1-PalcS/M-T2-PaldA expression cassette (al…Available sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB4636
Plasmid#215264PurposeModule for the expression of HISPS under the ed light regulated Etr8:CMVmin promoter and H3H under the copper inducible CBS:mDFR promoter and the constitutive expression of LUZ, CPH,EGFP and p19DepositorInsertEtr4:mDFR:HISPS-CBS4x:mDFR:H3HSF-LUZ-CPH-EGFP-p19
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB4541
Plasmid#215260PurposeModule for the expression of the N. nambi HISPS gene under the regulation of the Red light-regulated Etr4:mDFR promoter and TNOS in and the constitutve expression of CPH, LUZ, H3H, EGFP and p19.DepositorInsertEtr4:mDFR:HISPS-SF-CPH-SF-LUZ-H3H-EGFP-p19
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
PGK-LCB3_v1.2-NotchGal4VP64-T2A-mCherry (pZYW047)
Plasmid#194230PurposeLentiviral expression vector expressing LCB3 SynNotch and mCherry transduction markerDepositorInsertPGK-LCB3-SynNotch-T2A-mCherry
UseLentiviralTagsExpressionMutationE45D mutation in LCB3PromoterPGKAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1a-LCB3_v1.2-PDGFR-T2A-mCherry (pZYW049)
Plasmid#194232PurposeLentiviral expression vector expressing LCB3 on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction markerDepositorInsertEF1a-LCB3-PDGFR TMD-T2A-mCherry
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast coUCK2 D62A
Plasmid#211530PurposeExpresses codon optimized catalytic mutant UCK2DepositorInsertUCK2 D62A (UCK2 Human)
UseRetroviralTagsExpressionMammalianMutationD62A (catalytic mutant)PromoterAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA335
Plasmid#216069PurposeFragmid fragment: (2A Selection) Stop; IRES2; Start; HygroR; StopDepositorHas ServiceCloning Grade DNAInsertStop; IRES2_v1; Start; HygroR_v1.1; Stop
UseFragmentTagsExpressionMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-effector-ncRNA-rrt-His8
Plasmid#194089PurposeExpresses Ec86 operon (effector, ncRNA and reverse transcriptase) in Escherichia coliDepositorInsertEc86 effector-ncRNA-rrt
UseTagsHis8ExpressionMutationPromoterT7Available sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA020
Plasmid#216004PurposeFragmid fragment: (Cas protein) binds PP7 stem loopsDepositorHas ServiceCloning Grade DNAInsertPP7 Core Protein (PCP)_v1.1
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA366
Plasmid#215970PurposeFragmid fragment: (Pol 2 promoter) Gal4-induced expressionDepositorHas ServiceCloning Grade DNAInsert5xUAS_v1; 5xUAS_v1; 5xUAS_v1; 5xUAS_v1; Hsp70_v2; Mhc_v1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only
