19,585 results

1. pFREE_Amp

   Plasmid

   #92051

   Purpose

   Allows inducible expression of gRNAs and Cas9 for plasmid curing.

   Depositor

   Morten Norholm

   Article

   Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

   Inserts

   Cas9
   gRNA array
   

   Use

   CRISPR

   Promoter

   pRhamBAD and ptet

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL4.10/RSV_SF3B3-Xon_Luciferase

   Plasmid

   #174659

   Purpose

   Plasmid containing the SF3B3-Xon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-xon by LMI070.

   Depositor

   Beverly L. Davidson

   Article

   Monteys et al Nature. 2021 Jul 28. pii: 10.1038/s41586

   Insert

   Xon cassette
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   Kozak ATG initiation sequence in middle exon

   Promoter

   RSV

   Available Since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLEX307-APN-puro

   Plasmid

   #158454

   Purpose

   Constitutive lentiviral expression of human APN.

   Depositor

   Sho Iketani, Alejandro Chavez

   Insert

   APN (ANPEP Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. HyperADAR control

   Plasmid

   #166969

   Purpose

   Express the Hyperactive catalytic domain of Drosophila ADAR and linker at the 5' end of this domain, in the MSCV-IRES-GFP

   Depositor

   Michael Kharas

   Article

   Nguyen et al Nat Commun. 2020 Apr 24;11(1):2026. doi:

   Insert

   linker-hyperADAR
   

   Use

   Retroviral

   Tags

   linker-HyperADAR

   Expression

   Mammalian

   Promoter

   MSCV

   Available Since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSin-EF2-Nanog-Pur

   Plasmid

   #16578

   Depositor

   James Thomson

   Article

   Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

   Insert

   Homo sapiens Nanog homeobox (NANOG Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. Flag‐mTim3

   Plasmid

   #49208

   Purpose

   expresses Flag tagged mouse Tim3

   Depositor

   Lawrence Kane

   Article

   Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

   Insert

   Tim3 (Havcr2 Mouse)
   

   Tags

   FLAG and signal sequence

   Expression

   Mammalian

   Mutation

   no Tim3 signal sequence

   Promoter

   EF1a

   Available Since

   Dec. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAD48-daf-2 RNAi

   Plasmid

   #34834

   Depositor

   Cynthia Kenyon

   Article

   Dillin et al Science. 2002 Oct 25;298(5594):830-4.

   Insert

   daf-2 (daf-2 Nematode)
   

   Use

   RNAi

   Expression

   Bacterial

   Available Since

   June 11, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pCMV3TAG8li-hRNF160-3FLAG

   Plasmid

   #159138

   Purpose

   human RNF160 (LTN1, ZNF294) FLAG mammalian expression vector.

   Depositor

   Martin Dorf

   Article

   Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

   Insert

   listerin E3 ubiquitin protein ligase 1 (LTN1 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV human IRS-1 CAAX

   Plasmid

   #11238

   Depositor

   Ronald Kahn

   Article

   Kriauciunas et al Mol Cell Biol. 2000 Sep . 20(18):6849-59

   Insert

   IRS-1 (IRS1 Human)
   

   Tags

   CAAX

   Expression

   Mammalian

   Mutation

   C-terminal prenylation motif makes IRS-1 localize…

   Available Since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

10. mADAM17 in pcDNA3.1

    Plasmid

    #19141

    Depositor

    Zena Werb

    Article

    Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

    Insert

    ADAM17 (Adam17 Mouse)
    

    Expression

    Mammalian

    Available Since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pUDP002

    Plasmid

    #103872

    Purpose

    Broad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Inserts

    hph
    Spcas9 D147Y P411T
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Yeast

    Mutation

    D147Y P411T

    Promoter

    Arxula adeninivorans TEF1 and Ashbya gossypii (Er…

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. En1

    Plasmid

    #34993

    Depositor

    Malin Parmar

    Article

    Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

    Insert

    Homeobox protein engrailed-1 (En1 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGreenPuro-shRNA-Stx3S-C4

    Plasmid

    #99742

    Purpose

    3rd generation lentiviral vector expresses shRNA against human Stx3S with a GFP reporter.

    Depositor

    Thomas Weimbs

    Article

    Giovannone et al J Biol Chem. 2018 Apr 13;293(15):5478-54

    Insert

    shRNA against Stx3S
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    H1

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFREE_Cm

    Plasmid

    #92052

    Purpose

    Allows inducible expression of gRNAs and Cas9 for plasmid curing.

    Depositor

    Morten Norholm

    Article

    Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

    Inserts

    Cas9
    gRNA array
    

    Use

    CRISPR

    Promoter

    pRhamBAD and ptet

    Available Since

    Aug. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGH020_sgRNA_G418-GFP

    Plasmid

    #85405

    Purpose

    hu6 driven sgRNA vector with G418 and GFP selectable markers

    Depositor

    Michael Bassik

    Article

    Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    G418 resistance and GFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmCherry-Paxillin

    Plasmid

    #80029

    Purpose

    Mammalian expression of mouse Paxillin

    Depositor

    Chinten James Lim

    Insert

    Paxillin (Pxn Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE-GATA3

    Plasmid

    #116747

    Purpose

    Lentiviral expression of GATA3

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    GATA3 (GATA3 Human)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(-) MID49 4xMyc Hisx6

    Plasmid

    #44596

    Depositor

    David Chan

    Article

    Loson et al Mol Biol Cell. 2013 Jan 2.

    Insert

    MiD49 (Mief2 Mouse)
    

    Tags

    4xMyc and 6xHis

    Expression

    Mammalian

    Available Since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. mscv2.2-NAIP5

    Plasmid

    #60205

    Purpose

    Mouse NAIP5 (B6 allele), expressed under the constitutive, moderate-level retroviral LTR promoter for mammalian cell expression; IRES-GFP reporter downstream of NAIP5

    Depositor

    Russell Vance

    Article

    Tenthorey et al Mol Cell. 2014 Apr 10;54(1):17-29. doi:

    Insert

    NAIP5
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHACK-GAL4>QF2

    Plasmid

    #80275

    Purpose

    GAL4->QF2 HACK construct. Contains 5' and 3' GAL4 homology arms and gRNAs.

    Depositor

    Christopher Potter

    Article

    Lin et al Genetics. 2016 Jun 22. pii: genetics.116

    Insert

    QF2
    

    Expression

    Insect

    Available Since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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