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L2E-2

Plasmid

#21194

Depositor

Paul Khavari

Article

Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

Insert

Mek2 (Esr1, MAP2K2 Mouse, Human)

Use

Retroviral

Tags

Expression

Mutation

deletion of residues EQQKKRLE, S222D S226D in Me…

Promoter

Available since

July 24, 2009

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag MKK7a2

Plasmid

#14621

Depositor

Roger Davis

Article

Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

Insert

MKK7a2 (Map2k7 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
mTFP1-Profilin-10

Plasmid

#55506

Purpose

Localization: Cytoskeleton, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Profilin

Use

Tags

mTFP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV5 N539

Plasmid

#25972

Depositor

Rob Lewis

Article

Joneson et al J Biol Chem. 1998 Mar 27. 273(13):7743-8

Insert

Kinase suppressor of Ras 1 (Ksr1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Deleted amino acids 540-873

Promoter

Available since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pO-macs-ISH

Plasmid

#15856

Depositor

Peter Mombaerts

Article

Hirota et al Mol Cell Neurosci. 2007 Apr . 34(4):679-

Insert

Olfactory specific medium-chain acyl CoA synthetase (Acsm4 Mouse)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

March 31, 2009

Availability

Academic Institutions and Nonprofits only
HOXA4 (murine) pET

Plasmid

#8488

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXA4 (Hoxa4 Mouse)

Use

Tags

His and T7

Expression

Bacterial

Mutation

Thr-2 changed to Ala-2 to form better ribosomal b…

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pBROAD3-Flag-mMcl-1

Plasmid

#32986

Depositor

Joseph Opferman

Article

Stewart et al Mol Cell Biol. 2010 Jun;30(12):3099-110.

Insert

Mcl-1 (Mcl1 Mouse)

Use

Tags

3XFlag

Expression

Mammalian

Mutation

Promoter

ROSA

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pVenus-PICK1-shRNA

Plasmid

#72575

Purpose

Expresses shRNA against PICK1 as well as the marker Venus fluorescent protein

Depositor

Victor Anggono, Richard Huganir

Article

Anggono et al J Neurosci. 2011 Feb 9;31(6):2188-96. do

Insert

PICK1 (Pick1, Pick1 Rat, Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

H1 promoter

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only
L1

Plasmid

#21200

Depositor

Paul Khavari

Article

Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

Insert

Mek2 (Esr1, MAP2K2 Mouse, Human)

Use

Retroviral

Tags

Expression

Mutation

Mek2 K101A fused to ER*, catalytically inactive

Promoter

Available since

July 24, 2009

Availability

Academic Institutions and Nonprofits only
pGSTag DLC2

Plasmid

#24270

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

DLC2 (Dynll2 Mouse)

Use

Tags

Gst

Expression

Bacterial

Mutation

Promoter

Available since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
pMOR103-9-ISH

Plasmid

#15850

Depositor

Peter Mombaerts

Article

Hirota et al Mol Cell Neurosci. 2007 Apr . 34(4):679-

Insert

MOR103-9 (Olfr1348 Mouse)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

March 31, 2009

Availability

Academic Institutions and Nonprofits only
pENTR-L1-CAMKII(0.4)-R5

Plasmid

#32578

Depositor

Matthew Nolan

Article

White et al Front Mol Neurosci. 2011;4:8. Epub 2011

Insert

CAMKIIalpha promoter 0.4kb version

Use

Gateway cloning vector

Tags

Expression

Mutation

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP_mNTAQ1

Plasmid

#65486

Purpose

Mouse Ntaq1-EGFP reporter

Depositor

Alexander Varshavsky

Article

Wang et al Mol Cell. 2009 Jun 26;34(6):686-95. doi:

Insert

Ntaq1 (Wdyhv1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
pLPC MYC mTel2

Plasmid

#22803

Depositor

Titia de Lange

Article

Takai et al Cell. 2007 Dec 28. 131(7):1248-59.

Insert

Telomere Maintenance 2 (Telo2 Mouse)

Use

Retroviral

Tags

MYC

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pCR8/GW/TOPO-TBX20a

Plasmid

#98620

Purpose

Gateway entry TOPO TA cloning vector for TBX20a

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

Tbx20a (Tbx20 Mouse)

Use

Topo ta cloning vector

Tags

Expression

Mutation

Promoter

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS Wnt-6HA

Plasmid

#17378

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-6HA (Wnt6 Mouse)

Use

Tags

HA

Expression

Bacterial

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
mADAM8 deltaMP in pQCXIP cFLAG

Plasmid

#19149

Depositor

Zena Werb

Article

Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

Insert

ADAM8 (Adam8 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

delta 228-397

Promoter

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
RALB

Plasmid

#55658

Purpose

expression of mouse RALB

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RALB (Ralb Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pET23a-MmUBB

Plasmid

#69556

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

UBB (Ubb Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
Anti-Kv2.1 K+ channel pS603 [L61/14R]

Plasmid

#140063

Purpose

Mammalian Expression Plasmid of anti-Kv2.1 K+ channel pS603 (Mouse). Derived from hybridoma L61/14.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Kv2.1 K+ channel pS603 (Rat) recombinant mouse monoclonal antibody (Kcnb1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
mCardinal-Talin-H-18

Plasmid

#62741

Purpose

Localization: Talin Head Domain, H, Excitation:604, Emission:659

Depositor

Michael Davidson

Insert

Talin

Use

Tags

mCardinal

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
DONSON_pCSdest

Plasmid

#53870

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

DONSON

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
TRIN-E-shTAF12.376

Plasmid

#105573

Purpose

Dox-inducible mirE TAF12 shRNA/dsRED expression with GFP marker and neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF12 shRNA

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only
pSuper-PlexinB1

Plasmid

#115174

Purpose

shRNA against rodent PlexinB1

Depositor

Suzanne Paradis

Article

McDermott et al Mol Cell Neurosci. 2018 Jul 4;92:50-66.

Insert

shRNA targeting Plxnb1

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-RGS14 [N133/21.2R]

Plasmid

#114519

Purpose

Mammalian Expression Plasmid of anti-RGS14 (Rat). Derived from hybridoma N133/21.2.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-RGS14 (Rattus norvegicus) recombinant mouse monoclonal antibody (Rgs14 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pUAST YFP mouse Rab35 DN

Plasmid

#46016

Depositor

Matthew Scott

Article

Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

Insert

Rab35 (Rab35 Mouse)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

S22N (dominant negative)

Promoter

GAL4

Available since

July 8, 2013

Availability

Academic Institutions and Nonprofits only
pPERPlucFL

Plasmid

#14811

Depositor

Tyler Jacks

Article

Reczek et al Mol Cancer Res. 2003 Dec . 1(14):1048-57

Insert

PERP (Perp Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial

Mutation

Promoter

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 Hmga2 m4

Plasmid

#14791

Depositor

David Bartel

Article

Mayr et al Science. 2007 Mar 16. 315(5818):1576-9.

Insert

Hmga2 m4 (Hmga2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Hmga2 m4 mutant (see article and author's ma…

Promoter

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pX330-sgRNA_Drosha_3

Plasmid

#79862

Purpose

CRISPR/Cas9 DROSHA

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

sgRNA Drosha

Use

CRISPR and Mouse Targeting

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only
mEos2-CAF1-C-10

Plasmid

#57351

Purpose

Localization: Chromatin Assembly Factor 1, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

CAF1

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCDNA3-HA/Bmal L115E

Plasmid

#47345

Purpose

PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Use

Tags

Ha

Expression

Mammalian

Mutation

L115E

Promoter

CMV

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pMSCV-puro-mMcl-1 KR

Plasmid

#32981

Depositor

Joseph Opferman

Article

Stewart et al Mol Cell Biol. 2010 Jun;30(12):3099-110.

Insert

Mcl-1 (Mcl1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

All Lys to Arg

Promoter

LTR

Available since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA-h-mmPtch1-B-L235C-HA

Plasmid

#120906

Purpose

Expresses mmPtch1-B (L235C)

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291, L…

Promoter

CMV

Available since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
3156 pBS Bad cDNA

Plasmid

#8752

Depositor

Stanley Korsmeyer

Article

Yang et al Cell 1995 Jan 27;80(2):285-91.

Insert

BAD (Bad Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
FUGW-H1-Syndecan-1 shRNA (UTR)

Plasmid

#40623

Depositor

Sally Temple

Article

Wang et al PLoS One. 2012;7(8):e42883. Epub 2012 Au

Insert

Syndecan-1 shRNA(UTR) (Sdc1 Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only
pVLAD6-mmPtch1-B-NNQ-TEV-SBP

Plasmid

#120887

Purpose

BacMam vector for Ptch1-B NNQ variant expression

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Use

Tags

SBP

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291, N…

Promoter

CMV

Available since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3-myc- mCE(Del 25C)

Plasmid

#82472

Purpose

Expresses myc tag and mRNA capping enzyme without 25 amino acid from C-terminal

Depositor

Daniel Schoenberg

Article

Mukherjee et al PLoS Biol. 2014 Aug 19;12(8):e1001933. d

Insert

myc-mCE-C terminal deleted (Rngtt Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pScalps_puro_mIL-2Rα RK

Plasmid

#59920

Purpose

Expresses mouse interleukin-2 receptor alpha chain mutated

Depositor

Silvia Monticelli

Article

Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

Insert

interleukin 2 receptor alpha (Il2ra Mouse)

Use

Lentiviral

Tags

Expression

Mutation

changed sequence of the intracellular (C-terminal…

Promoter

Available since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only
Nrp2.1-AP-His

Plasmid

#71972

Purpose

Expresses the extracellular region of the Neuropilin 2, isoform 1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Nrp2.1 (Nrp2 Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-mE6-AP/2525

Plasmid

#11467

Depositor

Allan Weissman

Article

Hatakeyama et al J Biol Chem. 1997 Jun 13. 272(24):15085-

Insert

mE6-AP (Ube3a Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

n/a

Promoter

Available since

April 5, 2006

Availability

Academic Institutions and Nonprofits only
pX330-sgRNA_Dicer_1

Plasmid

#68807

Purpose

specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of Dicer1 knockout mESCs.

Depositor

Constance Ciaudo

Article

Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

Insert

sgRNA mouse Dicer1

Use

CRISPR and Mouse Targeting

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PJ14

Plasmid

#25912

Depositor

Kenneth Marcu

Article

Lang et al Nucleic Acids Res. 1982 Jan 22. 10(2):61

Insert

Ig germline mu switch region

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 4, 2010

Availability

Academic Institutions and Nonprofits only
pAcUW31-M10.5/human b2m

Plasmid

#11521

Depositor

Pamela Bjorkman

Article

Olson et al PLoS Biol. 2005 Aug . 3(8):e257.

Insert

M10.5 (H2-M10.4 Mouse)

Use

Tags

His

Expression

Insect

Mutation

Promoter

Available since

April 28, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-miR30_shBrd9_1116-PGK-NeoR-IRES-GFP

Plasmid

#75130

Purpose

Retroviral expression plasmid encoding shBrd9_1116 (targeting murine Brd9)

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBrd9_1116

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
pENTR-TER+-shLKB1-Ms

Plasmid

#61234

Purpose

Entry clone encoding shRNA to mouse LKB1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

shLkb1 (Ms/Hu) (STK11, Stk11 Mouse, Human)

Use

Entr clone

Tags

Expression

Mutation

Promoter

H1/TO

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pInducer21 Flag-Sesn1b

Plasmid

#61865

Purpose

Dox inducible lentivirial expression of Flag-Sesn1b (mouse)

Depositor

Ming Li

Article

Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

Insert

Sesn1 (Sesn1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

none

Promoter

Available since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only
miR-141 reporter

Plasmid

#67632

Purpose

To quantify miR-141 function

Depositor

Judy Lieberman

Article

Le et al J Clin Invest. 2014 Dec;124(12):5109-28.

Insert

2 binding sites for miR-141

Use

Luciferase

Tags

Expression

Mutation

Promoter

SV40

Available since

July 23, 2015

Availability

Academic Institutions and Nonprofits only
pLVTHM-shREV3-4

Plasmid

#38033

Depositor

Rolf Stahel, Emanuela Felley-Bosco

Article

Knobel et al Neoplasia. 2011 Oct;13(10):961-70.

Insert

shREV3-4 (Rev3l, Rev3l, REV3L Rat, Mouse, human, Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pMyt1(502-623)

Plasmid

#22655

Depositor

Lynn Hudson

Article

Romm et al J Neurochem. 2005 Jun . 93(6):1444-53.

Insert

Myelin Transcription Factor 1 (Myt1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

contains only first 1/2 of Myt1 central domain (C…

Promoter

Available since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
ATF4 19: (SL)RLuc.5’ATF4.GFP

Plasmid

#21865

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Stem Loop upstream of ATF4

Promoter

Available since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only

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