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178,651 results

Showing: 421 - 450 of 178651 results

  4. pEP4 E02S CK2M EN2L

    Plasmid
    #20924
    Purpose
    used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Klf4 and Myc; Nanog and Lin28
    Depositor
    James Thomson
    Publication
    Yu et al Science. 2009 Mar 26.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Oct4 and Sox2; Nanog and Lin28; Klf4 and Myc (MYC, SOX2, KLF4, NANOG, POU5F1, LIN28A Human)
    Use
    Episomal/ebna
    Available since
    May 20, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    20924-plasmid-map-sequence-id-326611

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  6. pBBR1MCS-2

    Plasmid
    #85168
    Purpose
    Mobilisable shuttle and expression vector. Replicates in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Cloned genes driven by derepressed lac promoter.
    Depositor
    Kenneth Peterson
    Publication
    Kovach et al Gene. 1995 Dec 1;166(1):175-6.
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    Constitutive (derepressed P-lac)
    Available since
    Nov. 8, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    85168-plasmid-map-sequence-id-372970

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  8. pC015 - dLwCas13a-NF

    Plasmid
    #91905
    Purpose
    Expresses negative-feedback dLwCas13a for mammalian RNA targeting/imaging
    Depositor
    Feng Zhang
    Publication
    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLwCas13a
    Use
    CRISPR
    Tags
    NLS and eGFP, ZF CCR5TC, KRAB-A, NLS
    Expression
    Mammalian
    Mutation
    R474A and R1046A
    Promoter
    CAG
    Available since
    Oct. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    91905-plasmid-map-sequence-id-198453

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  12. pdCas9-humanized

    Plasmid
    #44246
    Purpose
    Expression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown
    Depositor
    Stanley Qi
    Publication
    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1
    Insert
    dead Cas9 with 3X NLS
    Use
    CRISPR and Retroviral; Vector
    Tags
    3x NLS
    Expression
    Mammalian
    Mutation
    D10A H840A (catalytically inactive)
    Promoter
    MSCV LTR promoter
    Available since
    April 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    44246-plasmid-map-sequence-id-60247

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  16. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA

    Plasmid
    #20298
    Purpose
    Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation
    Depositor
    Karl Deisseroth
    Has service
    AAV1, AAV5, AAV9, AAV PHP.eB, and AAV Retrograde
    Insert
    channelrhodopsin-2
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    humanized ChR2 gene with histidine 134 changed to…
    Promoter
    EF1alpha
    Available since
    May 13, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    20298-plasmid-map-sequence-id-191904

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  20. pEF.myc.ER-E2-Crimson

    Plasmid
    #38770
    Purpose
    In vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)
    Depositor
    Benjamin Glick
    Publication
    Strack et al Biochemistry. 2009 Sep 8;48(35):8279-81.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    E2-Crimson
    Tags
    ER retention signal (KDEL), ER signal sequence (M…
    Expression
    Mammalian
    Promoter
    EF-1a
    Available since
    Aug. 15, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    38770-plasmid-map-sequence-id-200379

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  22. pTriEx-HTNC

    Plasmid
    #13763
    Purpose
    For expression and purification of a His-TAT-NLS-Cre fusion; HIV-TAT promotes cellular uptake of recombinant Cre into mammalian cells.
    Depositor
    Klaus Rajewsky
    Publication
    Peitz et al Proc Natl Acad Sci U S A. 2002 Apr 2. 99
    Insert
    His-TAT-NLS-Cre
    Use
    Baculovirus
    Tags
    His Tag, NLS, and TAT
    Expression
    Bacterial
    Promoter
    p10 promoter
    Available since
    April 12, 2007
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    13763-plasmid-map-sequence-id-364424

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  27. pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase

    Plasmid
    #119816
    Purpose
    3rd generation lentiviral plasmid for expression of EGFP Firefly Luciferase fusion protein in mammalian cells
    Depositor
    Ethan Abel
    Publication
    Lorenzatti Hiles et al Nat Protoc. 2019 Mar;14(3):738-755. doi:
    Inserts
    Luciferase
    Puromycin
    Use
    Lentiviral and Luciferase
    Tags
    EGFP and V5
    Expression
    Mammalian
    Mutation
    Stop codon removed in the frame of the V5 frame v…
    Promoter
    CMV/TO and Human Phosphoglycerate kinase
    Available since
    Jan. 2, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    119816-plasmid-map-sequence-id-372950

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Showing: 421 - 450 of 178651 results