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Addgene

We narrowed to 3,314 results for: GCT

Showing: 561 - 600 of 3314 results
  1. 1114H

    Plasmid
    #200640
    Purpose
    An. gambiae transgenesis plasmid. Expresses gRNAs targeting B2-tubulin, ZPG, and DSXF. Crossing to Cas9 generates sterile males
    Depositor
    Insert
    gRNA targeting DSXF, ZPG, and B2-tubulin. Actin5c-CFP and Vasa2-EYFP reporters.
    Use
    CRISPR
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Dec. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. pPBO.ACT004

    Plasmid
    #182714
    Purpose
    Constituitvely expressed CasRx crRNA cloning vector, extended 3' terminator region
    Depositor
    Insert
    CasRx crRNA cloning backbone
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Catalytically deactivated R295A, H300A, R849A, H8…
    Promoter
    pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)
    Available since
    Aug. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. pSLQ1371-sgYAP-10

    Plasmid
    #121424
    Purpose
    sgYAP-10 sequence: GTGCTGTCCCAGATGAACGTC. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.
    Insert
    sgYAP-10 (GTGCTGTCCCAGATGAACGTC)
    Use
    CRISPR and Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    mouse U6
    Available since
    April 22, 2019
    Availability
    Academic Institutions and Nonprofits only
  4. pJRH-1345 U6-B2M sgRNA Gag-Cas9 v2

    Plasmid
    #201916
    Purpose
    Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)
    Depositor
    Inserts
    Gag-Cas9 v2
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and Human U6
    Available since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. pPBO.ACT006

    Plasmid
    #182716
    Purpose
    Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region, with eGFP for cloning
    Depositor
    Insert
    CasRx crRNA cloning backbone
    Use
    CRISPR
    Tags
    GFP in cloning site of crRNA for easier screening…
    Expression
    Bacterial
    Mutation
    Catalytically deactivated R295A, H300A, R849A, H8…
    Promoter
    pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)
    Available since
    Aug. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. LLP773_pLenti-6xHRBKET-sgRNA

    Plasmid
    #211766
    Purpose
    Multiplexed gRNA plasmid targeting six different gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1), with mCherry selection
    Depositor
    Insert
    gRNAs against six different human gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    pCMV and U6
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 561 - 600 of 3314 results