We narrowed to 8,780 results for: construct
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Plasmid#209976PurposeContains Level 0 Part: replication origin (SH71rep) for the construction of Level 1 plasmidsDepositorInsertreplication origin (SH71_rep)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
p3E-MCS
Plasmid#75174PurposeMultiple cloning site for insertion of gene of interest for C-terminal fusions.DepositorInsertMultiple Cloning Site
UseThree prime entry vector for multisite gateway th…Promotern/aAvailable SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CMV-mCh-kCAAX
Plasmid#112267PurposeITRACK Acceptor ConstructDepositorInsertmCherry with KRas membrane targeting domain
TagsmCherryExpressionMammalianPromoterCMVAvailable SinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLPO1
Plasmid#209975PurposeContains Level 0 Part: replication origin (256rep) for the construction of Level 1 plasmidsDepositorInsertreplication origin (256_rep)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
LZF43: Cre-on CAG-CheRiff-CFP (DIO)
Plasmid#104114PurposesynOptopatch constructDepositorInsertCheRiff-CFP
UseLentiviralExpressionMammalianPromoterCAGAvailable SinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSicoR PGK puro
Plasmid#12084PurposeCre-regulated lentiviral shRNA vector. Cre addition causes both puromycin and shRNA to be recombined out of the construct, turning OFF shRNA expression.DepositorTypeEmpty backboneUseCre/Lox, Lentiviral, and RNAiExpressionMammalianAvailable SinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_Z18-PDGFR_EF1a_eGFP
Plasmid#186304PurposePlasmid for inducible mammalian cell expression of the Z18 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction.DepositorInsertsZ18-PDGFR
eGFP
TagsHA, mycExpressionMammalianAvailable SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-COX8 MTS-3xFlag-TALE array-TadA8e(V106W, V28F)-P2A-mCherry-UTR
Plasmid#235203PurposePlasmids for constructing mitoBEs v2DepositorInsertCOX8 MTS-3xFlag-TALE array-TadA8e(V106W, V28F)-P2A-mCherry-UTR
ExpressionMammalianAvailable SinceJuly 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
Burden Monitor lambda version
Plasmid#66073PurposeThe burden monitor was cloned inside plasmid pAH63 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cellsDepositorInsertGFP
Available SinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAGM4723:TpCC_Urease
Plasmid#85982PurposeGolden Gate Level 2 construct encoding Cas9-YFP and 2 urease-targeting gRNAsDepositorInsertsCas9
Urease-targeting gRNA 1
Urease-targeting gRNA 2
UseCRISPR; Diatom (t. pseudonana)TagsNLS and YFPPromoterFCP and U6Available SinceJan. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
EcPhnD90.L1∆∆AD.L297RL301R.pRSET
Plasmid#33376Purposea single-wavelength phosphonate sensor constructed from E. coli PhnD and cpGFPDepositorInsertphnD based Phosphonate Biosensor (phnD E. coli)
Tags6xHISExpressionBacterialMutationcpGFP146 variant from GCaMP2 inserted in place of…PromoterT7Available SinceJan. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L1-PGK-PuroTK-L2
Plasmid#141005PurposeMutisite Gateway mediated vector constructionDepositorInsertPuroTK
ExpressionMammalianAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_Z17-PDGFR_EF1a_mCherry
Plasmid#186303PurposePlasmid for inducible mammalian cell expression of the Z17 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction.DepositorInsertsZ17-PDGFR
mCherry
TagsHA, mycExpressionMammalianAvailable SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-G12-CheRiff-T2A-mCherry
Plasmid#170275PurposeCheRiff-T2A-mCherry construct expression in mammlian cells. pAAV vector. Reporter plasmid.DepositorInsertCheRiff-T2A-mCherry
UseAAVPromoter11xUAS with TATA-box minimal promoterAvailable SinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBad-mAmetrine
Plasmid#18083PurposeBacterial expression of mAmetrine fluorescent proteinDepositorInsertmAmetrine fluorescent proteins
TagsHisExpressionBacterialMutationThe fluorescent protein was inserted between XhoI…Available SinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSLHDR03_mCherry_tCD19_unique guide
Plasmid#124062PurposeConstruction of 1st donor for scarless genome editing with unique guideDepositorInsertsmCherry
tCD19
UseMouse Targeting; Human targetingPromoterHuman UbC modifiedAvailable SinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV_S34F_U2AF1
Plasmid#176592PurposeAdenoviral construct to change S to F at 34th amino acid (S34F) in human U2AF1DepositorInsertU2AF1 (U2AF1 Human)
UseAAVAvailable SinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0059 PbuCas13 (B03) His6-TwinStrep-SUMO-BsaI
Plasmid#115209PurposeExpresses PbuCas13b from a SUMO-tagged bacterial expression constructDepositorInsertPbuCas13
TagsSUMOExpressionBacterialAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_AP4-PDGFR_EF1a_eBFP2
Plasmid#186306PurposePlasmid for inducible mammalian cell expression of the AP4 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.DepositorInsertsAP4-PDGFR
eBFP2
TagsHA, mycExpressionMammalianAvailable SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKL001
Plasmid#98917PurposeConstitutively expresses the CaPR construct, the voltage indicator PROPS tethered to the calcium sensor GCaMP6f, under the Sigma 70 promoter in E. coli.DepositorInsertCaPR
ExpressionBacterialPromoterBBa_J23118 - Strong ConstitutiveAvailable SinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
PS Intein/C1A Bid
Plasmid#242025PurposePS Intein Bid construct containing the C1A mutation in gp41-1, which abolishes its autocatalytic splicing activity.DepositorInsertPS Intein/C1A Bid
ExpressionMammalianPromoterCMVAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTet-On-TAP
Plasmid#59017PurposeConstruct allows tetracycline inducible expression of a protein of interest fused to a tandem affinity purification tag (TAP) in toxoplasma gondii.DepositorTypeEmpty backboneUseToxoplasma expressionTagsCalmodulin Binding Peptide fused with a TEV Cleav…PromoterRPS13 with Tet Operators InsertedAvailable SinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_sg83-PDGFR_EF1a_iRFP670
Plasmid#186311PurposePlasmid for inducible mammalian cell expression of the sg83 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line constructionDepositorInsertssg83-PDGFR
iRFP670
TagsHis, mycExpressionMammalianAvailable SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_sg88-PDGFR_EF1a_eBFP2
Plasmid#186312PurposePlasmid for inducible mammalian cell expression of the sg88 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.DepositorInsertssg88-PDGFR
eBFP2
TagsHis, mycExpressionMammalianAvailable SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_P9-PDGFR_EF1a_mPlum
Plasmid#186307PurposePlasmid for inducible mammalian cell expression of the P9 helixCAM and constitutive expression of an identifying fluorescent protein (mPlum). Flanked with PiggyBac sites for easy line construction.DepositorInsertsP9-PDGFR
mPlum
TagsHA, mycExpressionMammalianAvailable SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_AP10-PDGFR_EF1a_mOrange
Plasmid#186308PurposePlasmid for inducible mammalian cell expression of the AP10 helixCAM and constitutive expression of an identifying fluorescent protein (mOrange). Flanked with PiggyBac sites for easy line constructionDepositorInsertsAP10-PDGFR
mOrange
TagsHA, mycExpressionMammalianAvailable SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_P3-PDGFR_EF1a_iRFP670
Plasmid#186305PurposePlasmid for inducible mammalian cell expression of the P3 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line construction.DepositorInsertsP3-PDGFR
iRFP670
TagsHA, mycExpressionMammalianAvailable SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
Opto-ACTU−
Plasmid#198869PurposeOptogenetic recruitment construct to increase inner membrane surface chargeDepositorInsertRad17 polyanionic tail (-14)
UseSynthetic BiologyTagsCRY2PHR-mCherryExpressionMammalianPromoterCMVAvailable SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMemoryArray
Plasmid#60585PurposeAn array constructed by concatenating the attB/P sites of the 11 phage integrases and can be used to distinguish 2048 possible combinations of events.DepositorInsertMemoryArray
ExpressionBacterialAvailable SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
6x Halo-EGFP- 2x PACT
Plasmid#107265Purpose6x Halo-EGFP- 2x PACT construct downstream of T7 promotor for in vitro transcriptionDepositorInsert6x Halo-EGFP- 2x PACT
TagsGFPExpressionMammalianPromoterT7Available SinceJuly 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC0065 Pin2Cas13 (B09) His6-TwinStrep-SUMO-BsaI
Plasmid#115216PurposeExpresses Pin2Cas13b from a SUMO-tagged bacterial expression constructDepositorInsertPin2Cas13
TagsSUMOExpressionBacterialAvailable SinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
LZF39: Cre-off hSyn-CheRiff-CFP (FAS)
Plasmid#104118PurposesynOptopatch constructDepositorInsertCheRiff-CFP
UseLentiviralExpressionMammalianPromoterhuman synapsin IAvailable SinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC0058 PinCas13 (B02) His6-TwinStrep-SUMO-BsaI
Plasmid#115208PurposeExpresses PinCas13b from a SUMO-tagged bacterial expression constructDepositorInsertPinCas13
TagsSUMOExpressionBacterialAvailable SinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMS31: pTwinStrep-SUMO-PmcTnsAB
Plasmid#168164PurposePmcTnsAB expression construct for protein purification.DepositorInsertPmcCAST TnsAB
Tags6X His, Twin Strep and SUMOExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWM_12x601_193 NRL + TetO
Plasmid#157812PurposeEcoRV digestion produces 12x601 chromatin assembly construct with central TetO in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_tetO
ExpressionBacterialAvailable SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-PT2A-GFP-Tdtomato-iRFP670
Plasmid#111812PurposeTricistronic construct: Co-express three fluorescent proteins by P2A and then T2ADepositorInsertsGFP
Tdtomato
iRFP670
UseCloning intermediateAvailable SinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuroATT
Plasmid#47539PurposeRetroviral Gateway destination vector constructed by inserting Reading Frame Cassette A into pMSCVpuro (Clontech) using the Gateway Vector Conversion System (Invitrogen)DepositorTypeEmpty backboneUseRetroviralExpressionMammalianAvailable SinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pME-Trpa1b-2A-mCherry
Plasmid#106291PurposeGateway middle entry construct containing zebrafish Trpa1b, 2A self-cleaving peptide and mCherryDepositorAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC0057 BzoCas13 (B01) His6-TwinStrep-SUMO-BsaI
Plasmid#115207PurposeExpresses BzoCas13b from a SUMO-tagged bacterial expression constructDepositorInsertBzoCas13
TagsSUMOExpressionBacterialAvailable SinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
p12x601
Plasmid#157785PurposeEcoRV digestion produces 12x601 chromatin assembly construct with central TetO following PEG removal of plasmid backboneDepositorInsertpMD2.G(spei/spei)&12x601_tetO
ExpressionBacterialAvailable SinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only