We narrowed to 17,071 results for: Por

1. pAAV-GfaABC1D-GRAB_eCB2.0

   Plasmid

   #164609

   Purpose

   Express the endocannabinoid sensor GRAB_eCB2.0 in astrocytes

   Depositor

   Yulong Li

   Article

   Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

   Insert

   Endocannabinoid sensor GRAB_eCB2.0
   

   Use

   AAV

   Mutation

   S383T

   Promoter

   GfaABC1D

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKanCMV-mRuby3-18aa-actin

   Plasmid

   #74255

   Purpose

   Mammalian expression of N-terminal fusion of mRuby3 to actin

   Depositor

   Michael Lin

   Article

   Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

   Insert

   actin
   

   Tags

   mRuby3

   Expression

   Mammalian

   Promoter

   CAG and CMV

   Available Since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAVS1-PuroR Xlone eGFP

   Plasmid

   #179837

   Purpose

   Doxycycline-inducible expression of eGFP

   Depositor

   Xiaoping Bao

   Article

   Jung et al ACS Synth Biol. 2022 May 24. doi: 10.102

   Insert

   eGFP
   

   Use

   CRISPR, Synthetic Biology, and TALEN ; Donor plas…

   Expression

   Mammalian

   Promoter

   TRE3GS

   Available Since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a(+)::MBP-NL3F10H

   Plasmid

   #141291

   Purpose

   MBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

   Depositor

   Andrew Millar

   Article

   Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

   Insert

   MBP-NLUC3F10H
   

   Use

   Luciferase

   Tags

   Flag (x3), His (x10), and MBP

   Expression

   Bacterial

   Mutation

   Engineered for high stability (t1/2 = 11.5 days a…

   Promoter

   promoter for bacteriophage T7 RNA polymerase

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDN80

   Plasmid

   #61349

   Purpose

   LINuS-flex: modular LINuS construct enabling introduction of any protein encoding sequence, NES and NLS. The sequence of interest replaces the LexA DBD-MBP-VP64 fragment.

   Depositor

   Roland Eils, Barbara Di Ventura

   Article

   Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

   Type

   Empty backbone

   Tags

   AsLOV2 wt and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-MyosinVb-Ctail

   Plasmid

   #110170

   Purpose

   Dominant negative effect by competing with MyosinVb C-terminal tail interactions

   Depositor

   Jose Esteban

   Article

   Correia et al Nat Neurosci. 2008 Apr;11(4):457-66. doi

   Insert

   Myosin Vb (Myo5b Rat)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   amino acids 1262-1670

   Promoter

   CMV

   Available Since

   May 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCVL.SFFV.Kozak.HA.NLS.Y2AniK227M.IRES.BFP

   Plasmid

   #45579

   Purpose

   Expresses K227M nickase variant of I-Ani I nuclease in mammalian cells with BFP tracker

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Insert

   coY2 Ani K227M
   

   Use

   Lentiviral

   Tags

   HA, IRES BFP, and NLS

   Expression

   Mammalian

   Mutation

   co indicates codon optimization for mammalian exp…

   Promoter

   SFFV

   Available Since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDAS12342_PEAR-GFP_2in1

   Plasmid

   #177184

   Purpose

   PEAR-GFP plasmid with a pegRNA that targets its own plasmid

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Inserts

   EGFP split with an intron between amino acids 95-96
   pegRNA targeting its own plasmid
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   disrupted 5' splice site

   Promoter

   CMV and U6

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR221_SLC22A1

   Plasmid

   #132034

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

   Insert

   SLC22A1 (SLC22A1 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 25, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pAD-CMV-STAT5A-CMV-GFP

    Plasmid

    #83251

    Purpose

    Adenoviral expression of STAT5A with co-expression of GFP

    Depositor

    Andrew Brooks

    Article

    Legrand et al J Invest Dermatol. 2016 Sep;136(9):1781-

    Inserts

    Stat5a (Stat5a Mouse)
    eGFP
    

    Use

    Adenoviral

    Tags

    FLAG

    Promoter

    CMV

    Available Since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGREAT1

    Plasmid

    #170889

    Purpose

    GoldenBraid Double Transcriptional Unit - pNOS_E-Luc_NOSt+pNOS_Red-F_NOSt

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    E-Luc
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Plant

    Mutation

    c.375C>T silent mutation to remove BbsI site (…

    Promoter

    pNOS

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-Nuc-ExRai-AMPKAR

    Plasmid

    #192450

    Purpose

    ExRai-AMPKAR targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    ExRai-AMPKAR-NLS
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKanCMV-mClover3-18aa-Tubulin

    Plasmid

    #74253

    Purpose

    Mammalian expression of N-terminal fusion of mClover3 to Tubulin

    Depositor

    Michael Lin

    Article

    Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

    Insert

    Tubulin
    

    Tags

    mClover3

    Expression

    Mammalian

    Promoter

    CAG and CMV

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-ChRmine-mRuby2-ST

    Plasmid

    #227777

    Purpose

    Cation channelrhodopsin ChRmine fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic excitation

    Depositor

    Hillel Adesnik

    Article

    Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

    Insert

    ChRmine-mRuby2-ST
    

    Expression

    Mammalian

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-CD81-(TEV)-tTA

    Plasmid

    #162598

    Purpose

    human CD81-TEV cleavage site-tTA

    Depositor

    Masaharu Somiya

    Article

    Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

    Insert

    human CD81 (CD81 Human)
    

    Tags

    TEV protease cleavage site and tTA

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLYS1-EMRE/RNAi resistant

    Plasmid

    #50056

    Purpose

    Expression of human EMRE in mammalian cells with silent mutation making it resistant to RNAi

    Depositor

    Vamsi Mootha

    Article

    Sancak et al Science. 2013 Nov 14.

    Insert

    EMRE-RNAi resistant (SMDT1 Human)
    

    Expression

    Mammalian

    Mutation

    RNAi resistant, silent mutant

    Available Since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDB22

    Plasmid

    #61342

    Purpose

    Mammalian expression of mCherry fused to LINuS, a light-inducible nuclear localization signal (cMycP1A NLS/PKIt NES)

    Depositor

    Roland Eils, Barbara Di Ventura

    Article

    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

    Insert

    mCherry
    

    Tags

    GS linker-AsLov2-cMyc1A NLS and PKIt NES

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-h-mmPtch1-FL

    Plasmid

    #120889

    Purpose

    Expresses wildtype Ptch1 for function assays

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    ptch1 (Ptch1 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. A3Bctd-max

    Plasmid

    #198888

    Purpose

    Express A3Bctd-max BE4max-based base editor

    Depositor

    Reuben Harris

    Article

    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

    Insert

    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)
    

    Tags

    6x His and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 3xflag-APEX2-NES

    Plasmid

    #164623

    Purpose

    Expresses a nuclear exporting signal (NES) tagged with APEX2 and 3xflag

    Depositor

    Mark Cookson

    Article

    Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

    Insert

    NES
    

    Tags

    3xFLAG and APEX2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pFastBac-Dual_FluA/H7N9

    Plasmid

    #229860

    Purpose

    pFastBac Dual vector for co-expression of FluPolA/H7N9 polymerase heterotrimer (PA/PB1/PB2)

    Depositor

    Stephen Cusack

    Article

    Kouba et al Cell Rep. 2023 Jan 31;42(1):111901. doi:

    Inserts

    PA_H7N9-FluA
    PB1_H7N9-FluA
    PB2_H7N9-FluA
    

    Tags

    2x-Streptag and 8xHis-tag

    Expression

    Insect

    Available Since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMIR-His-Halo-Tev-PTEN

    Plasmid

    #58241

    Purpose

    expresses halo tagged PTEN protein

    Depositor

    Yimon Aye

    Article

    Fang et al J Am Chem Soc. 2013 Oct 2;135(39):14496-

    Insert

    phosphatase and tensin homolog (PTEN Human)
    

    Tags

    His-Halo-Tev

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAD-CMV-STAT5B-CMV-GFP

    Plasmid

    #83257

    Purpose

    Adenoviral expression of STAT5B with co-expression of GFP

    Depositor

    Andrew Brooks

    Article

    Legrand et al J Invest Dermatol. 2016 Sep;136(9):1781-

    Inserts

    Stat5b (Stat5b Mouse)
    eGFP
    

    Use

    Adenoviral

    Promoter

    CMV

    Available Since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRR-GR-5Z

    Plasmid

    #23060

    Depositor

    Charles Miller

    Article

    Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

    Insert

    GR (NR3C1 Human)
    

    Expression

    Yeast

    Available Since

    March 16, 2010

    Availability

    Academic Institutions and Nonprofits only

25. pX330 EGFR-sgRNA

    Plasmid

    #188633

    Purpose

    A human codon-optimized SpCas9 and chimeric guide RNA targeting C-terminus of human EGFR

    Depositor

    Jared Toettcher

    Article

    Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-Dlx-FLEX-hM4Di-mCherry

    Plasmid

    #209701

    Purpose

    Expresses hM4Di-mCherry under the control of distalless homeobox 5 and 6 (mDlx) enhancer in Cre-recombinase-dependent manner

    Depositor

    Peer Wulff

    Article

    Rangel Guerrero et al Neuron. 2024 Apr 10:S0896-6273(24)00197-

    Insert

    hM4D(Gi)
    

    Use

    AAV

    Tags

    mCherry

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMRXIP-GFP-FAPP(PHD)

    Plasmid

    #221748

    Purpose

    Stable expression of GFP-FAPP(PHD) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    FAPP (PLEKHA3 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    amino acids 1–101

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFA-DAB2

    Plasmid

    #162690

    Purpose

    Expresses DabAB2 inorganic carbon transport complex

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    DabAB2 inorganic carbon transport complex
    

    Expression

    Bacterial

    Promoter

    PLtet0-1 promoter

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAC-aZEA

    Plasmid

    #53286

    Purpose

    Contains crtE, and crtB genes of Erwinia herbicola (Pantoea agglomerans) Eho10, crtI gene of Rhodobacter capsulatus, and lcyE cDNA of Arabidopsis thaliana and produces alpha-zeacarotene in E. coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

    Insert

    lcyE (LUT2 Mustard Weed)
    

    Use

    Low copy number bacterial cloning vector

    Promoter

    lac

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MBP-C5E-6XTUBE

    Plasmid

    #217628

    Purpose

    Bacterial expression of MBP tagged 6XTUBE

    Depositor

    Ethan Lee

    Article

    Kassel et al Sci Signal. 2023 Feb 7;16(771):eabn8372.

    Insert

    6XTUBE
    

    Expression

    Bacterial

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. AAV-syn-ChR2-Kv-P2A-H2b-mRuby2

    Plasmid

    #89570

    Purpose

    Channelrhodopsin localized to neuronal soma with simultaneous nuclear labeling

    Depositor

    McLean Bolton

    Article

    Baker et al Elife. 2016 Aug 15;5. pii: e14193. doi:

    Inserts

    Channelrhodopsin
    Histone 2B
    

    Use

    AAV

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    synapsin

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMXs_NMNAT3-FLAG

    Plasmid

    #133261

    Purpose

    Express FLAG-tagged NMNAT3 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    NMNAT3 (NMNAT3 Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pATP403red-hENT1

    Plasmid

    #166027

    Purpose

    Expresses human equilibrative nucleoside transporter 1 (hENT1)

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Insert

    human equilibrative nucleoside transporter 1 (SLC29A1 Human)
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    pTDH3

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pTD-C_eYFPTwinStrep

    Plasmid

    #45942

    Purpose

    expresses E.coli codon optimized EYFP-Twin-Strep fusion protein

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Insert

    synthetic eYFP (codon usage adapted to E.coli K12)
    

    Tags

    Twin-Strep-tag

    Expression

    Bacterial

    Promoter

    lacIq/Ptrc

    Available Since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTetOne-DsRed-cNLS

    Plasmid

    #138528

    Purpose

    Inducible expression of DsRed-Express2 with nuclear localization signal

    Depositor

    Christopher Grigsby, Ana Teixeira

    Article

    Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

    Insert

    DsRed-Express2
    

    Tags

    3x SV40 cNLS

    Expression

    Mammalian

    Promoter

    TRE3Gs

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pTD-C_sfYFPTwinStrep

    Plasmid

    #45943

    Purpose

    expresses P.putida codon optimized super-folder sfYFP-Twin-Strep fusion protein

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Insert

    synthetic sfYFP (codon usage adapted to P.putida KT2440)
    

    Tags

    Twin-Strep-tag

    Expression

    Bacterial

    Promoter

    lacIq/Ptrc

    Available Since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pJFRC-20XUAS-IVS-PhiC31

    Plasmid

    #133565

    Purpose

    Replace the 20XUAS promoter to express PhiC31 under the control of any promoter of interest.

    Depositor

    Tom Clandinin

    Article

    Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

    Insert

    PhiC31 Recombinase (int )
    

    Expression

    Insect

    Promoter

    20X UAS

    Available Since

    Oct. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Lenti-eCas9-sgMIB1

    Plasmid

    #140239

    Purpose

    Lentiviral vector expressing high-specificity eSpCas9(1.1) and a gRNA targeting human MIB1

    Depositor

    Urs Greber

    Article

    Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

    Insert

    sgRNA targeting human MIB1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pWZL N-Myc-hPOT1_deltaOB

    Plasmid

    #166408

    Purpose

    express hPOT1 ΔOB in mammalian cells

    Depositor

    Agnel Sfeir

    Article

    Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

    Insert

    POT1 ΔOB (POT1 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    ΔOB

    Available Since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDONOR-tagBFP-PSM-EGFP

    Plasmid

    #100603

    Purpose

    Donor scaffold with EGFP positive selection module, and tagBFP negative selection module

    Depositor

    Jens Schwamborn

    Article

    Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    EGFP-T2A-Puro and tagBFP

    Available Since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge