161,273 results

1. pT3-EF1A-mTert

   Plasmid

   #162555

   Purpose

   Overexpression of Tert

   Depositor

   Amaia Lujambio

   Article

   Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

   Insert

   Overexpression of Tert (Tert Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   EF1A

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKL123

   Plasmid

   #187889

   Purpose

   AAVS1 safe-harbor integration of dox-inducible NFIA-SOX9

   Depositor

   Martin Kampmann

   Article

   Leng et al Nat Neurosci. 2022 Nov;25(11):1528-1542.

   Insert

   NFIA-SOX9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. p5xQUAS-ZsGreen-P2A, α-cry:mCherry

   Plasmid

   #180477

   Purpose

   Inducible gene expression vector p5xQUAS-ZsGreen-P2A, α-cry:mCherry

   Depositor

   Hyunju Ro

   Article

   Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

   Inserts

   5x QUAS-ZsGreen-P2A
   mCherry
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)-HA-Flag-natT1R2

   Plasmid

   #113944

   Purpose

   mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R2 (TAS1R2 Human)
   

   Use

   Tags

   FLAG and Signal/leader sequence from influenza he…

   Expression

   Mammalian

   Mutation

   residues 22-839

   Promoter

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1 Neo(+) hSUMF1

   Plasmid

   #24386

   Depositor

   Carolyn Bertozzi

   Article

   Wu et al Proc Natl Acad Sci U S A. 2009 Mar 3. 10

   Insert

   hSUMF1 (SUMF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 2, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-Hs.cargo(Cre)

   Plasmid

   #174863

   Purpose

   Expresses human Peg10 cargoRNA encoding Cre recombinase

   Depositor

   Feng Zhang

   Article

   Segel et al Science. 2021 Aug 20;373(6557):882-889.

   Insert

   Cre flanked by HsPeg10 UTRs
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMV-NLS-AcrIIA4

   Plasmid

   #113037

   Purpose

   Expression of SV40NLS-AcrIIA4 in mammalian cells (CMV promoter driven)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   SV40 NLS-AcrIIA4
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIS0

   Plasmid

   #12178

   Purpose

   3'UTR segments of target genes can be inserted into this firefly luciferase reporter to test for their effects on protein production

   Depositor

   David Bartel

   Article

   Yekta et al Science. 2004 Apr 23. 304(5670):594-6.

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-C1-AKT-PH

   Plasmid

   #21218

   Depositor

   Tobias Meyer

   Article

   Raucher et al Cell. 2000 Jan 21. 100(2):221-8.

   Insert

   AKT-PH
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   PH domain of AKT. (Lab plasmid WC 0002).

   Promoter

   Available since

   July 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. Tag5Amyc-GSK3b CA

    Plasmid

    #16261

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

    Insert

    GSK3 beta (GSK3B Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Constitutively active: S9A

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJMP12042

    Plasmid

    #222354

    Purpose

    Tn7 integrative vector with PabstBR promoter expression cassette and aprR marker on Tn7 transposon; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.

    Depositor

    Jason Peters

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-26b-NbVHH05-ALFA-His

    Plasmid

    #171569

    Purpose

    Bacterial expression of ALFA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-ALFA-His)

    Depositor

    Norbert Perrimon, Hidde Ploegh

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    NbVHH05-ALFA-His (UBE2J1 Human)
    

    Use

    Tags

    ALFA-tag/His-tag and PelB signal peptide

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1_GEM2-Halo-FRB

    Plasmid

    #197056

    Purpose

    Expression of Synechococcus elongatus encapsulin Halo-FRB-fusion in mammalian cells

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Insert

    Encapsulin
    

    Use

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBABE-puro SHP2 WT

    Plasmid

    #8329

    Depositor

    Ben Neel

    Article

    Zhang et al Mol Cell Biol 2002 Jun;22(12):4062-72.

    Insert

    SHP2 (PTPN11 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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15. MMLVgag-3NES-Cre

    Plasmid

    #207252

    Purpose

    Generation of CreVLPs when used in conjunction with MMLV gag-pol during production

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    MMLVgag-3NES-Cre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-DN-hCUL4A-FLAG

    Plasmid

    #15821

    Depositor

    Wade Harper

    Article

    Jin et al Methods Enzymol. 2005 . 399():287-309.

    Insert

    Cullin4A (CUL4A Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Dominant negative: N440

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-GRAB_NE1m

    Plasmid

    #123308

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons

    Depositor

    Yulong Li

    Article

    Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

    Has Service

    AAV Retrograde and AAV9

    Insert

    GPCR activation based NE sensor GRAB_NE1m
    

    Use

    AAV

    Tags

    Expression

    Mutation

    contains a glycine-to-threonine mutation at posit…

    Promoter

    hSyn

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-mNaChBac-T2A-tdTomato

    Plasmid

    #60650

    Purpose

    Expresses mNaChBac-T2A-tdTomato

    Depositor

    Massimo Scanziani

    Article

    Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

    Insert

    mNaChBac-T2A-tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-Sport6-CD63-pHuji

    Plasmid

    #130902

    Purpose

    Expression of CD63-pHuji for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD63-pHuji (CD63 Human, synthetic)
    

    Use

    Tags

    pHuji

    Expression

    Mammalian

    Mutation

    pHuji inserted between Gln-36 and Leu-37 in extra…

    Promoter

    CMV

    Available since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAIP hIL4 co

    Plasmid

    #74169

    Purpose

    Lentiviral vector for expression of human codon optimized IL4 with puromycin resistance.

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    IL4 (IL4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    June 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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