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8,969 results

Showing: 4771 - 4800 of 8969 results

  4. 5'UTR CGG 99x FMR1-EGFP

    Plasmid
    #63091
    Purpose
    Contains expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 as found in the Fragile X Tremor Ataxia Syndrome (FXTAS). These repeats are in frame with EGFP.
    Depositor
    Nicolas Charlet-Berguerand
    Insert
    expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 (FMR1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63091-plasmid-map-sequence-id-459961

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  13. Gi1-CASE

    Plasmid
    #168120
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi1. Composed of the subunits G alpha i1 (GNAI1) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAI1, GNB1, GNG2 (GNB1, GNG2, GNAI1 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG2
    Expression
    Mammalian
    Mutation
    NLuc is inserted at A121/E122 within GNAI1
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168120-plasmid-map-sequence-id-332858

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  14. pEF1a-FB-dCas9-puro

    Plasmid
    #100547
    Purpose
    Expresses N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9 protein
    Depositor
    Jian Xu
    Article
    Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.
    Inserts
    N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9
    puromycin
    Use
    CRISPR
    Tags
    FLAG, Biotin-acceptor-site (Avitag)
    Expression
    Mammalian
    Promoter
    Human EF1a
    Available Since
    Sept. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    100547-plasmid-map-sequence-id-313417

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  23. IGI-P0492 pHR-dCas9-NLS-VPR-mCherry

    Plasmid
    #102245
    Purpose
    Lentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa.
    Depositor
    Jacob Corn
    Insert
    dCas9-VPR-mCherry
    Use
    CRISPR and Lentiviral
    Tags
    NLS-VPR-mCherry
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    102245-plasmid-map-sequence-id-354473

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  29. pDAC627

    Plasmid
    #195240
    Purpose
    Mammalian expression of Csm complex from separate promoters; Puro backbone (Used for RNA knockdown)
    Depositor
    Jennifer Doudna
    Article
    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103
    Insert
    FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; Puro
    Expression
    Mammalian
    Available Since
    Jan. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    195240-plasmid-map-sequence-id-457484

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Showing: 4771 - 4800 of 8969 results