165,775 results

1. pLVX-FLAG-mCherry-C1-centrin-1

   Plasmid

   #73333

   Purpose

   Lentiviral vector encoding centrin-1 with N-terminal FLAG and mCherry tags

   Depositor

   Manuel Thery

   Insert

   centrin-1 (CETN1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF0799

   Plasmid

   #141705

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ATF3 (ATF3 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. p5E-gfap

   Plasmid

   #82401

   Purpose

   Gateway-compatible zebrafish gfap promoter

   Depositor

   Judith Eisen

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   gfap promoter
   

   Available Since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK5_EGFP-BRD4-NUT

   Plasmid

   #238254

   Purpose

   For overexpression of EGFP-BRD4-NUT

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   EGFP-BRD4-NUT
   

   Expression

   Mammalian

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-C3-hYAP1

   Plasmid

   #17843

   Depositor

   Marius Sudol

   Article

   Basu et al Mol Cell. 2003 Jan . 11(1):11-23.

   Insert

   YAP (YAP1 Human)
   

   Tags

   EGFP

   Expression

   Bacterial and Mammalian

   Available Since

   May 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pINDUCER20-Src(WT)

   Plasmid

   #215463

   Purpose

   Dox-inducible lentiviral transfer vector with Src wild-type (WT)

   Depositor

   Johanna Ivaska

   Article

   Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

   Insert

   Src (SRC Chicken)
   

   Use

   Lentiviral

   Promoter

   mCMV, Doxycycline-inducible

   Available Since

   May 29, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. PB533_15F11-sfGFP

   Plasmid

   #167526

   Purpose

   H4K20me1-mintbody expression vector for mammalian cells

   Depositor

   Hiroshi Kimura

   Article

   Sato et al J Mol Biol. 2016 Oct 9;428(20):3885-3902

   Insert

   15F11-scFv
   

   Tags

   sfGFP

   Expression

   Mammalian

   Available Since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE

   Plasmid

   #125712

   Purpose

   Expresses an optimized mosquito OPN3 rhodopsin in-frame with mScarlet

   Depositor

   Ofer Yizhar

   Article

   Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

   Has Service

   AAV Retrograde, AAV1, and AAV5

   Insert

   eOPN3
   

   Use

   AAV

   Tags

   Rho1D4 and mScarlet

   Expression

   Mammalian

   Mutation

   deleted amino acids 331-429

   Promoter

   short version of αCamKinase II promoter (0.4kb)

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR221-FOS

   Plasmid

   #79511

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Human FOS CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   FOS (FOS Human)
   

   Use

   Multisite gateway entry clone

   Promoter

   no Promoter

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. dimericTomato-2xrGBD

    Plasmid

    #176098

    Purpose

    Visualization of active RhoA

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

    Insert

    rhotekin (Rtkn Mouse)
    

    Use

    Lentiviral

    Promoter

    CMVdel

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLViP-LifeAct-iRFP670

    Plasmid

    #229699

    Purpose

    Lentiviral expression of LifeAct-iRFP670 in mammalian cells

    Depositor

    Alpha Yap

    Article

    Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

    Insert

    Synthetic construct LifeAct-mTurquoise gene
    

    Use

    Lentiviral

    Tags

    iRFP670

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-RAM-d2TTA::TRE-EGFP-WPREpA

    Plasmid

    #84469

    Purpose

    pAAV-RAM-EGFP

    Depositor

    Yingxi Lin

    Article

    Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

    Insert

    EGFP
    

    Use

    AAV

    Promoter

    TRE

    Available Since

    Dec. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. ptyrEGFP-TYR

    Plasmid

    #32782

    Depositor

    Ruth Halaban

    Article

    Halaban et al Proc Natl Acad Sci U S A. 2000 May 23;97

    Insert

    Tyrosinase (TYR Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    Tyrosinase

    Available Since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

14. DHFR-SpCas9-DHFR

    Plasmid

    #85447

    Purpose

    Expresses SpCas9 fused to DHFR domain on both N- and C-termini in mammalian cells

    Depositor

    Amit Choudhary

    Article

    Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

    Insert

    DHFR-SpCas9-DHFR
    

    Use

    AAV

    Tags

    Destabilized domain (C-terminus version) of E.col…

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5/FRT/TO-3xFLAG-His6-LARP1

    Plasmid

    #233626

    Purpose

    Expression of Flag- and His-tagged LARP1

    Depositor

    Shintaro Iwasaki

    Article

    Shichino et al Nat Struct Mol Biol. 2024 Oct;31(10):155

    Insert

    LARP1 (LARP1 Human)
    

    Tags

    3FLAG-His6

    Expression

    Mammalian

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. lenti-EF1a-dCas9-KRAB-Puro

    Plasmid

    #99372

    Purpose

    3rd generation lenti vector encoding dCas9-KRAB with 2A puromycin resistance marker (EF1a-dCas9-KRAB-T2A-Puro-WPRE)

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Insert

    dCas9-KRAB-T2A-Puro
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti CMV Hygro DEST (w117-1)

    Plasmid

    #17454

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Hygro

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral; Destination vector

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBabe-Kras G12C

    Plasmid

    #58901

    Purpose

    Expresses human Kras with a G12C mutation

    Depositor

    Channing Der

    Insert

    KRAS (KRAS Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    G12C mutation

    Available Since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. p5E-3x125bp-mnx1

    Plasmid

    #169043

    Purpose

    5' entry vector for Gateway cloning for expression in spinal motor neurons using 3 tandem repeats of the zebrafish mnx1 enhancer.

    Depositor

    Paola Arlotta

    Article

    Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

    Insert

    Mnx1 enhancer (3x) (mnx1 Zebrafish)
    

    Use

    Gateway entry cloning vector

    Promoter

    3xMnx1

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. EcMscL

    Plasmid

    #92418

    Purpose

    E. coli Mechanosensitive Channel of Large Conductance

    Depositor

    Douglas Rees

    Insert

    MscL (mscL E. coli)
    

    Tags

    6x Histidine Tag and Thrombin Cleavage Site betwe…

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. His-FLAG-Tev_ACTB (beta-actin)

    Plasmid

    #188453

    Purpose

    Express in mammalian cells human beta-actin (ACTB) with N-terminal 6xHis tag, FLAG tag, and TEV protease site.

    Depositor

    Roberto Dominguez

    Article

    Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

    Insert

    ACTB (ACTB Human)
    

    Tags

    6xHis-FLAG

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. WT GBA pcDNA3.1

    Plasmid

    #188580

    Purpose

    Ectopic expression of wild-type GBA

    Depositor

    Anthony Schapira

    Article

    Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

    Insert

    GBA (GBA1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. PGC-1 alpha promoter luciferase delta CRE

    Plasmid

    #8888

    Depositor

    Bruce Spiegelman

    Article

    Handschin et al Proc Natl Acad Sci U S A. 2003 Jun 10. 1

    Insert

    PGC-1 alpha promoter dCRE (Ppargc1a Mouse)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Site-directed mutagenesis to remove the CREB bind…

    Available Since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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24. pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)

    Plasmid

    #196702

    Purpose

    Plasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.

    Depositor

    Karlene Cimprich

    Article

    Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

    Insert

    human RNaseH1 wild type
    

    Expression

    Mammalian

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. pIRES-EGFP-puro

    Plasmid

    #45567

    Depositor

    Michael McVoy

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    IRES-EGFP-Puro

    Expression

    Mammalian

    Promoter

    Phcmv (strong human cytomegalovirus immediate ear…

    Available Since

    June 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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26. FRB-mCherry-FTH1

    Plasmid

    #100749

    Purpose

    Part of FerriTag expression system. Encodes human Ferritin with an FRB domain and mCherry tag

    Depositor

    Stephen Royle

    Article

    Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1

    Insert

    ferritin heavy chain 1 (FTH1 Human)
    

    Tags

    FRB and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCK002 pME mCerulean

    Plasmid

    #195956

    Purpose

    Gateway pME fluorophore

    Depositor

    Alexa Burger, Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    mCerulean
    

    Use

    Other

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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28. dCas13d-dsRBD

    Plasmid

    #154938

    Purpose

    BPNLS-dRfxCas13d-dsRBD-BPNLS-HA

    Depositor

    Alice Ting

    Article

    Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

    Insert

    BPNLS-dRfxCas13d-dsRBD-BPNLS-HA
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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29. pDEST14-Cys-SpyCatcher002

    Plasmid

    #102829

    Purpose

    Bacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagents

    Depositor

    Mark Howarth

    Article

    Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

    Insert

    Cys-SpyCatcher002
    

    Tags

    GSSGS, His6, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Cysteine residue in N-terminal region

    Promoter

    T7

    Available Since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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30. pcDNA4TO-H2B-SunTag24x_v1

    Plasmid

    #60911

    Purpose

    Enocdes a histone H2B fused to the SunTag for single molecule imaging

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    H2B
    

    Expression

    Mammalian

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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