19,190 results

1. pGL3B-1081

   Plasmid

   #18759

   Depositor

   Clark Distelhorst

   Article

   Wang et al Oncogene. 2006 Mar 23. 25(13):1903-13.

   Insert

   thioredoxin-interacting protein promoter (Txnip Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 4, 2008

   Availability

   Academic Institutions and Nonprofits only

2. v3em-Cterm-PE6c-dualU6-Rosa26-twinPE-attB

   Plasmid

   #207861

   Purpose

   AAV genome encoding C-terminal PE6c and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   NpuC-Cterm-PE6c-dualU6-Rosa26-twinPE-attB
   

   Use

   AAV

   Tags

   Expression

   Mutation

   See Manuscript

   Promoter

   Cbh

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDUAL SRBI (GFP)

   Plasmid

   #86979

   Purpose

   Lentiviral expression construct encoding SR-B1 and GFP from separate promoters

   Depositor

   Joe Grove

   Insert

   SCARB1 (SCARB1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV/hPGK

   Available since

   Feb. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFG2

   Plasmid

   #87250

   Purpose

   gene for resistance to nourseothricin (Nat) inserted next to the parS segments (INT). Also known as pANCH1-Nat

   Depositor

   Kerstin Bystricky

   Article

   Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

   Insert

   Nat
   

   Use

   Tags

   parS segments

   Expression

   Yeast

   Mutation

   Promoter

   TEF

   Available since

   March 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a 6xHis PreScission SARS-CoV-2 nsp13

   Plasmid

   #159390

   Purpose

   Expresses the SARS-CoV-2 nsp13 protein with an N-terminal His6 tag followed by an HRV 3C/PreScission cleavage site.

   Depositor

   Tarun Kapoor

   Article

   Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

   Insert

   N-terminal His tag PreScission SARS-CoV-2 nsp13, optimized for insect cell expression
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pX458-Ef1a-Cas9-H2B-GFP

   Plasmid

   #159654

   Purpose

   Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B GFP nuclear reporter.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pyEvolvR (enCas9-PolI5M)

   Plasmid

   #158073

   Purpose

   Expresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.

   Depositor

   John Dueber

   Article

   Tou et al ACS Synth Biol. 2020 Jun 16. doi: 10.102

   Insert

   enCas9-PolI5M
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLuc-CDS

   Plasmid

   #42100

   Depositor

   Kumiko Ui-Tei

   Article

   Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

   Insert

   ZEB1 (ZEB1 Human)
   

   Use

   Luciferase

   Tags

   Renilla luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-SypHluorin 4x (S4x)

   Plasmid

   #37005

   Depositor

   Stephen Heinemann, Yongling Zhu

   Article

   Zhu et al Neuron. 2009 Feb 12;61(3):397-411.

   Insert

   SypHluorin 4x (Syp Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Four copies of pHluorin inserted at site 184-185

   Promoter

   CMV

   Available since

   July 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDONR207 SARS-CoV-2 ORF3A

    Plasmid

    #141271

    Purpose

    Gateway-compatible Entry vector, with insert of ORF3A gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    ORF3A (ORF3a SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    April 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. MYC_pLX307

    Plasmid

    #98363

    Purpose

    Lentiviral expression of MYC

    Depositor

    William Hahn, Sefi Rosenbluh

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    MYC (MYC Human)
    

    Use

    Lentiviral

    Tags

    NA

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-Spike

    Plasmid

    #173921

    Purpose

    Expresses the original SARS-Cov-2 Spike Protein under a CMV Promoter

    Depositor

    Andreas Stuermer

    Insert

    SARS-2 Spike Protein (S SARS-COV-2, Human, Synthetic)
    

    Use

    Tags

    no tags, native protein

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    standard CMV

    Available since

    April 11, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. GFP-Ub KO

    Plasmid

    #11934

    Depositor

    Nico Dantuma

    Article

    Bergink et al Genes Dev. 2006 May 15. 20(10):1343-52.

    Insert

    Ubiquitin KO (RBX1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    All 7 lysines of ubiquitin mutated to arginines.

    Promoter

    Available since

    Aug. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AGTR1-Tango

    Plasmid

    #66222

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    AGTR1 (AGTR1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJFRC-MUH

    Plasmid

    #26213

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Aug 9. ():.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-Plk1(829)

    Plasmid

    #39845

    Depositor

    Jonathon Pines

    Article

    Lindon et al J Cell Biol. 2004 Jan 19;164(2):233-41.

    Insert

    Plk1 (PLK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pMK782 Septin6-GFP

    Plasmid

    #38296

    Depositor

    Matthew Krummel

    Article

    Gilden et al J Cell Biol. 2012 Jan 9;196(1):103-14.

    Insert

    Septin 6 (Septin6 Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    22 amino acid linker sequence (added by PCR)

    Promoter

    CMV

    Available since

    Nov. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFPN3-Sstr3

    Plasmid

    #35623

    Depositor

    Kirk Mykytyn

    Article

    Berbari et al Mol Biol Cell. 2008 Apr;19(4):1540-7. Ep

    Insert

    Sstr3 (Sstr3 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAG104 FAST

    Plasmid

    #130720

    Purpose

    Expresses FAST (also called YFAST) in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

    Insert

    FAST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p50 cFlag pcDNA3

    Plasmid

    #20018

    Depositor

    Stephen Smale

    Insert

    Nfkb1 (Nfkb1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Truncated at the p105 ligation site. It codes for…

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. HA-Sos2

    Plasmid

    #32921

    Depositor

    Dafna Bar-Sagi

    Article

    Yang et al J Biol Chem. 1995 Aug 4;270(31):18212-5.

    Insert

    SOS2 (SOS2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. EGFP-hPMCA2w/b

    Plasmid

    #47586

    Depositor

    Emanuel Strehler

    Article

    Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

    Insert

    PMCA2w/b (ATP2B2 Human)
    

    Use

    Tags

    EGFP-HA-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pC001 - huLshC2C2-MBP for bacterial expression

    Plasmid

    #79150

    Purpose

    Expresses human codon-optimized LshC2c2 for purification in E. coli

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

    Insert

    C2c2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PCP-GFP

    Plasmid

    #101161

    Purpose

    to epress PCP-GFP fusion protein

    Depositor

    Mazhar Adli

    Article

    Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

    Insert

    PCP-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Ubc

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. SpCas9-NG

    Plasmid

    #138566

    Purpose

    Expresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    SpCas9-NG
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…

    Promoter

    EFS

    Available since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. YFP-Parkin

    Plasmid

    #23955

    Purpose

    Mammalian expression of human Park2 fused to EYFP

    Depositor

    Richard Youle

    Article

    Narendra et al J Cell Biol. 2008 Dec 1. 183(5):795-803.

    Insert

    Park2 (PRKN Human)
    

    Use

    Tags

    eYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDest eGFP MBNL1 42 kDa

    Plasmid

    #61277

    Purpose

    Mammalian expression of EGFP-tagged human MBNL1

    Depositor

    Nicolas Charlet-Berguerand

    Insert

    MBNL1 (MBNL1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHR-TRE3G-dCas9-HaloTag

    Plasmid

    #169448

    Purpose

    expression of dCas9-HaloTag

    Depositor

    Stanley Qi

    Article

    Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

    Insert

    dCas9-HaloTag
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pKLV2.2-mU6gRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W

    Plasmid

    #72667

    Purpose

    Lentiviral dual CRISPR gRNA expression vector (mouse U6 and human U6 promoters)

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    mU6gRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3-DN-hCUL3-FLAG

    Plasmid

    #15820

    Depositor

    Wade Harper

    Article

    Jin et al Methods Enzymol. 2005 . 399():287-309.

    Insert

    Cullin3 (CUL3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Dominant negative: N418

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLV-puro-N-3HA-SREBP2-C-Flag

    Plasmid

    #134287

    Purpose

    Lentivector encoding 3XHA (N-term) and Flag (C-term)-tagged SREBP2

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    SREBP2 (Srebf2 Mouse)
    

    Use

    Lentiviral

    Tags

    3x HA and Flag

    Expression

    Mammalian

    Mutation

    mouse SREBP2 precursor

    Promoter

    CMV

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. GeNL/pcDNA3

    Plasmid

    #85200

    Purpose

    Green color variant of bright luminescent protein

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    Green enhanced Nano-lantern
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSH-EFIRES-B-Seipin-miniIAA7-mEGFP

    Plasmid

    #129719

    Purpose

    AAVS1 safe harbor targeting vector expressing miniIAA7-mEGFP tagged Seipin

    Depositor

    Elina Ikonen

    Article

    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

    Insert

    Seipin (BSCL2 Human)
    

    Use

    CRISPR and TALEN; Human safe harbor locus (a avs1…

    Tags

    miniIAA7-mEGFP

    Expression

    Mammalian

    Mutation

    silent mutations

    Promoter

    EF1a

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pINDUCER21-MYB

    Plasmid

    #51305

    Purpose

    Expression of MYB after doxycycline administration

    Depositor

    George Daley

    Article

    Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

    Insert

    v-myb avian myeloblastosis viral oncogene homolog (MYB Human)
    

    Use

    Lentiviral

    Tags

    n/a

    Expression

    Mutation

    full length ORF

    Promoter

    Available since

    June 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. HSF1-GFPN3

    Plasmid

    #32538

    Depositor

    Stuart Calderwood

    Article

    Wang et al Mol Cell Biol. 2003 Sep;23(17):6013-26.

    Insert

    HSF1 (HSF1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    see depositor comments below

    Promoter

    CMV

    Available since

    Oct. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCAT001

    Plasmid

    #171061

    Purpose

    Plasmid for expressing HIV-1 envelope glycoprotein

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Insert

    HIV-1 Env
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLPCX-NICD

    Plasmid

    #44471

    Depositor

    Ernesto Canalis

    Article

    Zanotti et al J Biol Chem. 2011 Feb 11;286(6):4576-88.

    Insert

    Notch intracellular domain (Notch1 Mouse)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Contains amino acid 1753 to C-Term

    Promoter

    CMV

    Available since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

38. pAC-Luc

    Plasmid

    #33154

    Depositor

    Michael Rosbash

    Article

    Kadener et al Genes Dev. 2007 Jul 1;21(13):1675-86. Ep

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Insect

    Mutation

    Promoter

    Actin 5C

    Available since

    Dec. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

39. pGH020_sgRNA_G418-GFP

    Plasmid

    #85405

    Purpose

    hu6 driven sgRNA vector with G418 and GFP selectable markers

    Depositor

    Michael Bassik

    Article

    Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    G418 resistance and GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDuBir-Lbu-dCas13a-avitag

    Plasmid

    #100817

    Purpose

    Dual expression of Lbu-dCas13a with a C-terminal avi-tag and BirA to biotinylate Lbu-dCas13a

    Depositor

    Mitchell O'Connell

    Article

    Tambe et al Cell Rep. 2018 Jul 24;24(4):1025-1036. d

    Insert

    Lbu_Cas13a_R472A_H477A_R1048A_H1053A
    

    Use

    Tags

    AviTag and His6-MBP

    Expression

    Bacterial

    Mutation

    R472A, H477A, R1048A, H1053A

    Promoter

    T7

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pAB1620 hU6-BpiI-Cas13bt3-DR

    Plasmid

    #176315

    Purpose

    hU6-BpiI-Cas13bt3-DR (crRNA expression)

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

    Insert

    Cas13bt3 crRNA direct repeat
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. PB-EF1a-TetOn3G

    Plasmid

    #104543

    Purpose

    PiggyBac vector encoding Tet-On 3G constitutively expressed by EF1a promoter

    Depositor

    David Vereide

    Article

    Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

    Insert

    Tet-On 3G
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pPRIME-TET-GFP-recipient

    Plasmid

    #11655

    Purpose

    3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with a tetracycline-responsive promoter (TET) controlling GFP expression

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. AICSDP-28:TUBA1B-mTagRFP-T

    Plasmid

    #101785

    Purpose

    Homology arms and mTagRFPT-linker sequence for N-terminus (second exon) tagging of human TUBA1B

    Depositor

    Allen Institute for Cell Science

    Insert

    TUBA1B Homology Arms with mTagRFPT-linker (TUBA1B Human)
    

    Use

    CRISPR; Donor template

    Tags

    mTagRFPT-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    Oct. 31, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

45. pTRC-APEX2

    Plasmid

    #72558

    Purpose

    Expression of cytosolic APEX2 in bacteria

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    APEX2
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    A134P relative to APEX

    Promoter

    Available since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pDEST17-UbE2L3

    Plasmid

    #15795

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2L3 (UBE2L3 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pCS2 HRS-RFP

    Plasmid

    #29685

    Depositor

    Edward De Robertis

    Article

    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

    Insert

    hepatocyte growth factor-regulated tyrosine kinase substrate (HGS Human)
    

    Use

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pAC150-PBLHL-4xHS-EF1a-DEST

    Plasmid

    #48234

    Purpose

    PiggyBac expression gateway destination vector with Insulator-flanked EF1a promoter gateway cassette and floxed Hygro selection marker

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Type

    Empty backbone

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pET11a-SUMO1

    Plasmid

    #53138

    Purpose

    E.coli expression of untagged mature human SUMO1 (amino acids 1-97)

    Depositor

    Frauke Melchior

    Article

    Pichler et al Cell. 2002 Jan 11;108(1):109-20.

    Insert

    SUMO1 (SUMO1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mature form (amino acids 1-97)

    Promoter

    T7

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pCT302

    Plasmid

    #41845

    Depositor

    Dane Wittrup

    Article

    Midelfort et al J Mol Biol. 2004 Oct 22;343(3):685-701.

    Insert

    4-4-20
    

    Use

    Tags

    HA and c-myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge