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Showing: 8401 - 8450 of 23360 results

  7. pYPQ136B2.0

    Plasmid
    #167160
    Purpose
    Golden Gate entry vector to express the 6th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    AtU3
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    167160-plasmid-map-sequence-id-331282

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  9. pUC_sgRNA

    Plasmid
    #68710
    Purpose
    Contains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.
    Depositor
    Roberto Docampo
    Article
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    68710-plasmid-map-sequence-id-123992

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  10. pRC319

    Plasmid
    #61272
    Purpose
    Also referred to as pΦRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA targeting the NDM-1 gene. Contains a ColE1 origin, kanamycin resistance cassette and f1 origin for packaging in M13 particles.
    Depositor
    Timothy Lu
    Article
    Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103
    Insert
    Cas9, tracrRNA, crRNA
    Use
    CRISPR
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    61272-plasmid-map-sequence-id-99585

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  15. pCMV-T7-SpCas9-BPNLS(SV40)-3xFLAG-P2A-mScarlet (MNW001)

    Plasmid
    #174091
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a C-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    Promoter
    pCMV and T7
    Available since
    Sept. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    174091-plasmid-map-sequence-id-341917

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  18. pAC-crRNA-Cm

    Plasmid
    #158711
    Purpose
    Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNA
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1
    Insert
    eforCP (for crRNA cloning)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    TetO
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    158711-plasmid-map-sequence-id-325466

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  22. pJAT21

    Plasmid
    #204309
    Purpose
    For generating targeting inversions. N-terminal split GFP, expressed in abdomen and mouthparts
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    ie1-GFPn
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ie1
    Available since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    204309-plasmid-map-sequence-id-405852

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  23. pJAT25

    Plasmid
    #204310
    Purpose
    For generating targeting inversions. C-terminal split GFP, expressed in abdomen and mouthparts.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    ie1-GFPc
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ie1
    Available since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    204310-plasmid-map-sequence-id-405854

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  24. pADH110

    Plasmid
    #90982
    Purpose
    NAT-marked pSNR52 promoter fragment for use in gRNA expression cassette stitching PCR; for use with pADH119, 139, or 147 to generate target-specific gRNA expression cassette.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Inserts
    NAT 2 of 2
    pSNR52
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90982-plasmid-map-sequence-id-180510

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  28. pELK3.1.0-gDNA

    Plasmid
    #112418
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ELK3
    Depositor
    Kevin White
    Insert
    ELK3 (ELK3 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112418-plasmid-map-sequence-id-230297

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  29. ACE2 g1 + Cas9

    Plasmid
    #153011
    Purpose
    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
    Depositor
    Jason Sheltzer
    Insert
    Cas9 + ACE2 gRNA (ACE2 Human)
    Use
    CRISPR and Lentiviral
    Tags
    TagBFP2
    Expression
    Mutation
    Promoter
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153011-plasmid-map-sequence-id-298673

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  32. pDB4282

    Plasmid
    #98701
    Purpose
    A plasmid containing the 3' portion of the ura4 marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-ura4 system
    Depositor
    Li-Lin Du
    Article
    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.
    Insert
    gRNA PCR template
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    98701-plasmid-map-sequence-id-182827

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  37. pDB4283

    Plasmid
    #98702
    Purpose
    A plasmid containing the 3' portion of the bsdMX marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-bsdMX system
    Depositor
    Li-Lin Du
    Article
    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.
    Insert
    gRNA PCR template
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    98702-plasmid-map-sequence-id-182828

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  39. pATF4.1.0-gDNA

    Plasmid
    #113772
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ATF4
    Depositor
    Kevin White
    Insert
    ATF4 (ATF4 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113772-plasmid-map-sequence-id-230558

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  43. pJAT27

    Plasmid
    #204308
    Purpose
    For generating targeting inversions. C-terminal split GFP, expressed in flight muscles.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    MHC-GFPc
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    MHC
    Available since
    Oct. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    204308-plasmid-map-sequence-id-411129

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  44. pJAT18

    Plasmid
    #204307
    Purpose
    For generating targeting inversions. N-terminal split GFP, expressed in flight muscles.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    MHC-GFPn
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    MHC
    Available since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    204307-plasmid-map-sequence-id-405848

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  45. pAAV-CMV-FLEX-SaCas9-U6-sgHtr2a

    Plasmid
    #159908
    Purpose
    Mutagenesis of Htr2a
    Depositor
    Larry Zweifel
    Insert
    Htr2a (Htr2a Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159908-plasmid-map-sequence-id-317314

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  50. pJUNB.1.0-gDNA

    Plasmid
    #112409
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor JUNB
    Depositor
    Kevin White
    Insert
    JUNB (JUNB Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112409-plasmid-map-sequence-id-230268

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Showing: 8401 - 8450 of 23360 results