We narrowed to 21,604 results for: ESC

1. pLBM0010

   Plasmid

   #172816

   Purpose

   Level 2 module containing HSP21-GFP

   Depositor

   Benjamin Field

   Article

   Velay et al Plant Methods. 2022 May 26;18(1):69. doi

   Insert

   [35S-P19][35S-CTPSSU-mHSP21-GFP]
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Membrane EGFP

   Plasmid

   #198056

   Purpose

   Plasma membrane localizing EGFP using Human LCK proto-oncogene sequence

   Depositor

   Amro Hamdoun

   Insert

   LCK membrane EGFP (LCK Synthetic, Human, A. victoria)
   

   Use

   Sea urchin, mammal, zebrafish

   Tags

   EGFP

   Expression

   Mutation

   Tandem repeat of N-terminal LCK membrane targetin…

   Promoter

   sp6

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Dendra2-Actin-C-18

   Plasmid

   #57701

   Purpose

   Localization: Actin, Excitation: 490 / 553, Emission: 507 / 573

   Depositor

   Michael Davidson

   Article

   McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

   Insert

   Actin (ACTB Human)
   

   Use

   Tags

   Dendra2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pAAV-CAG-miniGFP2-P2A-FusionRed

   Plasmid

   #188734

   Purpose

   Express miniGFP2 and FusionRed in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

   Insert

   miniGFP2
   

   Use

   AAV

   Tags

   FusionRed

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. SepOTSλ

   Plasmid

   #68292

   Purpose

   Phosphoserine orthogonal translation system (preferred variant)

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   SepRS9/EFSep21, 4xtRNA-Sep-A37
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pC1-HyPer-Red-C199S

   Plasmid

   #48252

   Purpose

   Hydrogen peroxide insensitive version of HyPerRed to be used as a pH control in HyPer experiments.

   Depositor

   Vsevolod Belousov

   Article

   Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

   Insert

   HYPER-RED-C199S
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AAV-Camk2-NES-Twitch-GR

   Plasmid

   #173044

   Purpose

   TnC-based calcium sensor with green-red FRET pair ratiometric readout

   Depositor

   Yiyang Gong

   Article

   Zhang et al Commun Biol. 2021 Jul 29;4(1):924. doi:

   Insert

   NES-Twitch-GR
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CamK2

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLenti-mCherry-CAAX

   Plasmid

   #129285

   Purpose

   Lentiviral plasmid for expression of fluorescent reporter mCherry targeted to the plasma membrane via CAAX polybasic sequence

   Depositor

   Carlos Carmona-Fontaine

   Article

   Janska et al Dis Model Mech. 2021 Aug 1;14(8). pii: 2

   Insert

   Fluorescent reporter mCherry fused to CAAX polybasic sequence
   

   Use

   Lentiviral

   Tags

   CAAX

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pmScarlet-H_NES_C1

   Plasmid

   #85061

   Purpose

   In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   NES
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Dendra2-Golgi-7

    Plasmid

    #57722

    Purpose

    Localization: Golgi, Excitation: 490 / 553, Emission: 507 / 573

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    Golgi (B4GALT1 Human)
    

    Use

    Tags

    Dendra2

    Expression

    Mammalian

    Mutation

    aa 1-82 of NM_001497.3

    Promoter

    CMV

    Available since

    Jan. 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pMAH-POLY

    Plasmid

    #64915

    Purpose

    Encodes a polyspecific aminoacyl-tRNA synthetase and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells

    Depositor

    Huiwang Ai

    Article

    Chen et al J Am Chem Soc. 2013 Oct 9;135(40):14940-

    Inserts

    Polyspecific aminoacyl-tRNA Synthetase
    Amber Suppression tRNA
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6/H1

    Available since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGreenPuro-shRNA-Stx3S-C4

    Plasmid

    #99742

    Purpose

    3rd generation lentiviral vector expresses shRNA against human Stx3S with a GFP reporter.

    Depositor

    Thomas Weimbs

    Article

    Giovannone et al J Biol Chem. 2018 Apr 13;293(15):5478-54

    Insert

    shRNA against Stx3S
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLV-REPORT (PGK) NanoLuc

    Plasmid

    #208576

    Purpose

    Empty NanoLuc Lentiviral reporter

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    NanoLuciferase
    d2eGFP
    Empty response element
    

    Use

    Lentiviral, Luciferase, and Synthetic Biology

    Tags

    3x SV40 NLS and PEST

    Expression

    Mammalian

    Mutation

    Promoter

    PGK and miniP

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLNCX2-mCherry-VPS4A

    Plasmid

    #115334

    Purpose

    expresses mCherry-VPS4A in mammalian cells

    Depositor

    Sanford Simon

    Article

    Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

    Insert

    VPS4A (VPS4A Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    silent mutation in position 4050 C to T

    Promoter

    CMV

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAV-Camk2-NES-Twitch-NR

    Plasmid

    #173043

    Purpose

    TnC-based calcium sensor with green-red FRET pair ratiometric readout

    Depositor

    Yiyang Gong

    Article

    Zhang et al Commun Biol. 2021 Jul 29;4(1):924. doi:

    Insert

    NES-Twitch-NR
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamK2

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. psiCHECK2-2A-3xFLAG-SBP-2A

    Plasmid

    #159584

    Purpose

    Luciferase reporter for ribosome pausing control

    Depositor

    Shintaro Iwasaki

    Article

    Han et al Cell Rep. 2020 May 5;31(5):107610. doi:

    Inserts

    2A-3xFLAG-SBP-2A
    EIF2S3 5UTR
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCas12f1

    Plasmid

    #171182

    Purpose

    Constitutive expression of cas12f1, arabinose-inducible expression of lambda recombinase, and IPTG-inducible expression of sgRNA for curing of pTarget12f1

    Depositor

    Kohsuke Honda

    Article

    Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124

    Insert

    Cas12f1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    cas9 promoter from Streptococcus pyogenes

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEDA5_GFPmut3_Y66H

    Plasmid

    #80085

    Purpose

    Positive control plasmid for Nicking Mutagenesis. Constitutively expresses a non-fluorescent variant of GFPmut3 (Y66H) that is mutated to fluorescent (H66Y) in the protocol.

    Depositor

    Timothy Whitehead

    Article

    Wrenbeck et al Nat Methods. 2016 Nov;13(11):928-930. do

    Insert

    GFPmut3_Y66H
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    changed Tyrosine 66 to Histidine

    Promoter

    proB

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pACT5FVMF(f)

    Plasmid

    #110549

    Purpose

    Control plasmid for titering FV by qPCR to determine the genome-containing particle number. This qPCR protocol is described in Hendrie et al., 2008

    Depositor

    David Russell

    Article

    Hendrie et al Mol Ther. 2008 Mar;16(3):534-40. doi: 10

    Insert

    EGFP, FV LTRs
    

    Use

    Qpcr control for titering foamy virus

    Tags

    Expression

    Mutation

    Promoter

    promoter: MLV LTR

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLP2

    Plasmid

    #209989

    Purpose

    Entry vector to store Level 0 resistance cassette parts and E. coli shuttle part (containing no E. coli ori)

    Depositor

    Thorsten Mascher, Georg Fritz

    Article

    Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pISFba1

    Plasmid

    #226820

    Purpose

    Expressing ISEre1 controled by pCas promoter, λ-red recombinase, SacB and ISFba1 guide RNA targeting pMB1 ori

    Depositor

    Sheng Yang

    Article

    Zhou et al Biotechnol J. 2025 Mar;20(3):e70005. doi

    Insert

    ISFba1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pISFba1reRNA

    Plasmid

    #226822

    Purpose

    Expressing ISFba1 guide RNA targeting maeB site controled by PJ23119

    Depositor

    Sheng Yang

    Article

    Zhou et al Biotechnol J. 2025 Mar;20(3):e70005. doi

    Insert

    ISFba1 none target guide RNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSL4393 (pClpX_Eco)

    Plasmid

    #200907

    Purpose

    Expresses human codon-optimized E. coli ClpX from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    EcoClpX
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pOEM1_pCMV:hShh-pPH:vsvged

    Plasmid

    #111156

    Purpose

    Baculovirus rescue vector, VSVGED pseudotype, hShh

    Depositor

    Elly Tanaka

    Article

    Nacu et al Nature. 2016 May 19;533(7603):407-10. do

    Insert

    hShh (SHH Human)
    

    Use

    Baculoviral rescue shuttle vector

    Tags

    Expression

    Insect and Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AAV.hSyn.GLPLight-ctr

    Plasmid

    #187468

    Purpose

    Expresses GLPLight-ctr in mammalian cells

    Depositor

    Tommaso Patriarchi

    Article

    Duffet et al Elife. 2023 Jun 2;12:RP86628. doi: 10.75

    Insert

    GLPLight-ctr
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mutation

    Promoter

    human Synapsin-1

    Available since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pNAS1B

    Plasmid

    #61968

    Purpose

    Duet vector for co-expression of GFP and mCherry from PBAD and PLtetO promoters, respectively; parent vector for split mCherry assembly vectors

    Depositor

    Lynne Regan

    Article

    Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

    Inserts

    mCherry
    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Serine 65 to Glycine (GGT), Serine 72 to Alanine …

    Promoter

    PBAD and PLtetO

    Available since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. 8xGliBS-IVS2-mCherry-NLS-Odc1-polyA-Tol2

    Plasmid

    #84603

    Purpose

    Tol2-based hedgehog signal reporter plasmid for zebrafish transgenesis

    Depositor

    James Chen

    Article

    Mich et al PLoS One. 2014 Jul 28;9(7):e103661. doi:

    Insert

    mCherry
    

    Use

    Zebrafish transgenesis

    Tags

    ODC1 destabilizing element

    Expression

    Mutation

    Promoter

    8xGliBS-delta-crystallin minimal promoter

    Available since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pScI_dCas9-CDA-UL

    Plasmid

    #108551

    Purpose

    Bacterial Target-AID UGI-LVA vector

    Depositor

    Akihiko Kondo

    Article

    Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

    Insert

    dCas9-PmCDA1-UGI-LVA
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    D10A and H840A for SpCas9

    Promoter

    lambda OR

    Available since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHR-H13LTat-Thy1.2-P2A-GFP-T2A-Nef

    Plasmid

    #126553

    Purpose

    Replication incompetent HIV with H13L Tat and GFP-t2a-Thy1.2 reporter

    Depositor

    Jonathan Karn

    Article

    Dobrowolski et al MBio. 2019 Mar 26;10(2). pii: mBio.00337

    Insert

    HIV-1 vector pNL4-3
    

    Use

    Lentiviral

    Tags

    Thy1.2 and GFP

    Expression

    Mutation

    Promoter

    HIV LTR

    Available since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. JH02

    Plasmid

    #73511

    Purpose

    mCherryo_PH pCFJ150 - contians C. elegans sequence optimized mCherry

    Depositor

    Bob Goldstein

    Article

    Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

    Insert

    pmex-5::mCherryo::PH::tbb-2
    

    Use

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    pmex-5

    Available since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. mTurquoise2-C1

    Plasmid

    #54842

    Purpose

    Localization: C1 Cloning Vector, Excitation: 434, Emission: 474

    Depositor

    Dorus Gadella, Michael Davidson

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Type

    Empty backbone

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. EBFP2-Mito-7

    Plasmid

    #55248

    Purpose

    Localization: Mitochondria, Excitation: 383, Emission: 448

    Depositor

    Michael Davidson

    Article

    Shaner et al J Cell Sci. 2007 Dec 15;120(Pt 24):4247-

    Insert

    Mito (COX8A Human)
    

    Use

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pAG385 bJun-NFAST-NLS-DEVDG-mCherry-NES

    Plasmid

    #130816

    Purpose

    Expresses bJun-NFAST-NLS-DEVDG-mCherry-NES in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

    Insert

    bJun-NFAST-NLS-DEVDG-mCherry-NES
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAJT194

    Plasmid

    #202293

    Purpose

    Type IIS cloning plasmid for LPattB5 integrations w/ OriT

    Depositor

    Christopher Voigt

    Article

    Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-Syn-ChR2(H134R)-GFP

    Plasmid

    #58880

    Purpose

    AAV expression of ChR2(H134R)-GFP under the Syn promoter

    Depositor

    Edward Boyden

    Article

    Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

    Has Service

    AAV Retrograde and AAV8

    Insert

    ChR2(H134R)-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    Syn

    Available since

    Aug. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAJM.336

    Plasmid

    #108528

    Purpose

    LacIAM + PTac-YFP Reporter. IPTG sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    LacIAM + PTac-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LacI Q18M A47V F161Y

    Promoter

    Available since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pNIR-FbGFPY58/C-PKI

    Plasmid

    #184685

    Purpose

    NIR-Fb-based PKA inhibitor

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    NIR-FbGFPY58/C-PKI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLV-EF1a-MinE-EGFP_IRES-Blast

    Plasmid

    #213113

    Purpose

    Expression of MinE-EGFP in mammalian cells

    Depositor

    Scott Coyle

    Article

    Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

    Insert

    MinE-EGFP (minE Escherichia coli)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3-Venus-StagRFP-AktAR

    Plasmid

    #173020

    Purpose

    Yellow-red FRET-based Akt/PKB activity reporter for multiplexed imaging applications.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

    Insert

    Venus-StagRFP-AktAR
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. FusionRed-Lifeact-7

    Plasmid

    #54778

    Purpose

    Localization: Actin, Excitation: 580, Emission: 608

    Depositor

    Michael Davidson

    Article

    Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

    Type

    Empty backbone

    Use

    Tags

    FusionRed-Lifeact

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

41. pCDH_Str-KDEL_neomycin

    Plasmid

    #65307

    Purpose

    Luminal ER hook only (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end
    

    Use

    Lentiviral

    Tags

    KDEL motif and Signal Sequence

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pRU324

    Plasmid

    #167691

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::Cas9i
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pSW068

    Plasmid

    #140036

    Purpose

    KO of PCC 11901 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoter

    Depositor

    Peter Nixon

    Article

    Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

    Insert

    Truncated thioesterase gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    cLac143

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pmTurquoise2-NES

    Plasmid

    #36206

    Purpose

    In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    NES
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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45. pCA24N_6xHis-TEV-rpsA

    Plasmid

    #83585

    Purpose

    Express E. coli ribosomal protein S1 with a TEV-cleavable, N-terminal His tag

    Depositor

    Nils Walter

    Article

    Lund et al Nucleic Acids Res. 2019 Dec 13. pii: 567

    Insert

    rpsA (rpsA Escherichia coli)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    p-T5 Lac

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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46. pAJM.847

    Plasmid

    #108524

    Purpose

    PhlFAM + PPhlF-YFP Reporter. DAPG sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    PhlFAM + PPhlF-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    PhlF: S50G S73I R95H A121V M137I N144D T155I R200C

    Promoter

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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47. MCP-GFP1-10 P2A PCP-GFP11

    Plasmid

    #162038

    Purpose

    Background-free mRNA labeling bipartite superfolder GFP system

    Depositor

    Hye Yoon Park

    Article

    Park et al RNA. 2020 Jan;26(1):101-109. doi: 10.126

    Insert

    MCP-GFP1-10 P2A PCP-GFP11
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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48. pP3-GFP

    Plasmid

    #53436

    Purpose

    Controls GFP expression from the P3 promoter cloned out of S. aureus RN4220 genome

    Depositor

    Cynthia Collins

    Article

    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

    Insert

    P3 promoter
    

    Use

    Synthetic Biology

    Tags

    none

    Expression

    Bacterial

    Mutation

    none

    Promoter

    P3

    Available since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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49. pFA6a-link-ymScarletI-URA3

    Plasmid

    #168055

    Purpose

    Replaces Addgene plasmid 118457. Yeast tagging vector to create a gene fusion with yeast optimized mScarletI with CaUra3 selection; Contains the linker region at the 5’ end of the FP

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymScarletI
    

    Use

    Tags

    ymScarletI

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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50. FL-mCherry

    Plasmid

    #78862

    Purpose

    DULIP negative control (prey) to exclude unspecific background binding

    Depositor

    Erich Wanker

    Article

    Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

    Insert

    mCherry
    

    Use

    Expression vector

    Tags

    Firefly luciferase, V5-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge