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We narrowed to 18,759 results for: nls

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Showing: 6881 - 6920 of 18759 results

  5. SPACE-VRQR (pRZ5137)

    Plasmid
    #140245
    Purpose
    CMV promoter expression plasmid for TadA*(V82G)-nCas9_VRQR-pmCDA1(R187W)-UGI-UGI-P2A-EGFP
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SPACE_VRQR
    Expression
    Mammalian
    Mutation
    V82G in TadA*, VRQR mutations in SpCas9, D10A in …
    Promoter
    CMV
    Available since
    June 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    140245-plasmid-map-sequence-id-275727

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  11. PB-XL-AncBE4max

    Plasmid
    #136254
    Purpose
    Doxycycline inducible piggyBAC plasmid for mammalian expression of cytosine base editor AncBE4max
    Depositor
    Zhaohui Ye
    Publication
    McGrath et al Nat Commun. 2019 Nov 25;10(1):5353. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AncBE4max_GFP
    Use
    CRISPR; Piggybac dna transposon
    Expression
    Mammalian
    Mutation
    The AncBE4max_GFP insert was PCR amplified from a…
    Promoter
    TRE3G
    Available since
    Jan. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    136254-plasmid-map-sequence-id-270570

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  21. pRS143

    Plasmid
    #122377
    Purpose
    CRISPRi plasmid for use in Candida albicans. Contains dCas9 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)
    Depositor
    Rebecca Shapiro
    Publication
    Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Available since
    May 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    122377-plasmid-map-sequence-id-241241

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  22. pHR-pSFFV-TAF15N-mCherry-sspB

    Plasmid
    #122436
    Purpose
    Expresses fusion of disordered protein TAF15(1-208), fluorescent protein mCherry, and SspB which upon light activation binds to iLID.
    Depositor
    Clifford Brangwynne
    Publication
    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.
    Insert
    TAF15 (TAF15 Human)
    Use
    Lentiviral
    Tags
    mCherry and sspB
    Expression
    Mammalian
    Mutation
    Contains only amino acids 1-208
    Promoter
    SFFV
    Available since
    May 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    122436-plasmid-map-sequence-id-243727

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  24. pKLV2-U6(gCD44v2)-EF1a-BFP-Puro-Cas9(FZ)

    Plasmid
    #117134
    Purpose
    All-in-one expression vector for Cas9 and gRNA against CD44
    Depositor
    Kosuke Yusa
    Insert
    gCD44 v2 (Cd44 Synthetic)
    Use
    CRISPR and Lentiviral
    Mutation
    Deleted BbsI site within WPRE, modified BFP
    Promoter
    human U6 promoter, human EF1a promoter
    Available since
    April 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    117134-plasmid-map-sequence-id-226805

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  28. pEG302-SunTag-FWAgRNA-4-22aa-TET1cd

    Plasmid
    #115344
    Purpose
    SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoter
    Depositor
    Steven Jacobsen
    Insert
    gRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    March 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    115344-plasmid-map-sequence-id-222905

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  29. pCAGG-NFIB2

    Plasmid
    #112700
    Purpose
    Expression of NFIB2 protein and GFP
    Depositor
    Richard Gronostajski
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nuclear Factor I B2 (Nfib Mouse)
    Tags
    GFP and HA
    Expression
    Mammalian
    Available since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    112700-plasmid-map-sequence-id-212390

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  30. pCAGG-NFIX1

    Plasmid
    #112702
    Purpose
    Expression of NFIX1 protein and GFP
    Depositor
    Richard Gronostajski
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nuclear Factor I X1 (Nfix Mouse)
    Tags
    GFP and HA
    Expression
    Mammalian
    Available since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112702-plasmid-map-sequence-id-212396

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  36. HRE:GFP-pGK:Cre

    Plasmid
    #68552
    Purpose
    Lentivirus expressing hypoxia-responsive GFP reporter and Cre
    Depositor
    Tyler Jacks
    Inserts
    Green Fluorescent Protein
    Cre
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    HRE and pGK
    Available since
    Aug. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    68552-plasmid-map-sequence-id-120714

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  37. pLenti-ABE7.10-P2A-Puro

    Plasmid
    #112674
    Purpose
    Lentivirus for constitutive expression of ABE7.10 (not codon optimized)
    Depositor
    Lukas Dow
    Insert
    ABE7.10
    Use
    Lentiviral
    Mutation
    D10A
    Promoter
    EF1s
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    112674-plasmid-map-sequence-id-215665

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  38. PB-tight-DDdCas9GFP-IRES-Neo

    Plasmid
    #102907
    Purpose
    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of dCas9-GFP followed by IRES-Neomycin selection cassette.
    Depositor
    Timo Otonkoski
    Publication
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DDdCas9GFP-IRES-Neo
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    TRE-tight
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102907-plasmid-map-sequence-id-198394

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Showing: 6881 - 6920 of 18759 results