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Showing: 8601 - 8650 of 22610 results

  2. pTF-lacZ-rfp

    Plasmid
    #153037
    Purpose
    CRISPR-Cas12a genome editing in E. coli. Contains a constitutively CRISPR array targeting the lacZ gene in E. coli genome and donor DNA to insert an constitutively expressed rfp reporter gene.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.
    Insert
    CRISPR array
    Expression
    Bacterial
    Available Since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    153037-plasmid-map-sequence-id-318855

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  25. pMAH-POLY

    Plasmid
    #64915
    Purpose
    Encodes a polyspecific aminoacyl-tRNA synthetase and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells
    Depositor
    Huiwang Ai
    Article
    Chen et al J Am Chem Soc. 2013 Oct 9;135(40):14940-
    Inserts
    Polyspecific aminoacyl-tRNA Synthetase
    Amber Suppression tRNA
    Tags
    None
    Expression
    Mammalian
    Promoter
    CMV and U6/H1
    Available Since
    June 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    64915-plasmid-map-sequence-id-142448

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  28. pLV-REPORT (PGK) NanoLuc

    Plasmid
    #208576
    Purpose
    Empty NanoLuc Lentiviral reporter
    Depositor
    David Bersten
    Article
    Allen et al bioRxiv 2023.11.21.568191
    Inserts
    NanoLuciferase
    d2eGFP
    Empty response element
    Use
    Lentiviral, Luciferase, and Synthetic Biology
    Tags
    3x SV40 NLS and PEST
    Expression
    Mammalian
    Promoter
    PGK and miniP
    Available Since
    April 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    208576-plasmid-map-sequence-id-413002

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  29. pCAG-GluA1-scFv-Halo-HRV-BiotinAP-HIS

    Plasmid
    #230058
    Purpose
    Expresses a single chain variable fragment (scFv) for anti-GluA1 fused to Halotag with an HRV (PreScission) protease cleavage site, biotin acceptor peptide and 6XHIS purification tag.
    Depositor
    Matthew Kennedy
    Insert
    Anti-GluA1 single chain variable fragment (scFv)
    Tags
    6X HIS, HRV 3C PreScission protease, HaloTag, and…
    Expression
    Mammalian
    Promoter
    Chicken beta actin (CAG)
    Available Since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    230058-plasmid-map-sequence-id-460119

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  30. pCAG-GluA2-scFv-Halo-HRV-BiotinAP-HIS

    Plasmid
    #230059
    Purpose
    Expresses a single chain variable fragment (scFv) for anti-GluA2 fused to Halotag with an HRV (PreScission) protease cleavage site, biotin acceptor peptide and 6XHIS purification tag.
    Depositor
    Matthew Kennedy
    Insert
    Anti-GluA2 single chain variable fragment (scFv)
    Tags
    6X HIS, HRV 3C PreScission protease, HaloTag, and…
    Expression
    Mammalian
    Promoter
    Chicken beta actin (CAG)
    Available Since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    230059-plasmid-map-sequence-id-460118

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  37. pCNA

    Plasmid
    #135191
    Purpose
    Arabinose inducible expression of lamba-Red recombinase proteins and I-CreI.
    Depositor
    Hongqing Fang
    Article
    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d
    Insert
    I-CreI lamba Red
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    None
    Promoter
    araBAD
    Available Since
    March 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    135191-plasmid-map-sequence-id-273788

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  39. pBDK

    Plasmid
    #135190
    Purpose
    Integration and excision of kanamycin cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection
    Depositor
    Hongqing Fang
    Article
    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d
    Insert
    KanR
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    cat
    Available Since
    March 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    135190-plasmid-map-sequence-id-273774

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  41. pUASTattb-cAMPr Y205A, R739A

    Plasmid
    #84933
    Purpose
    cAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R739A should increase affinity for cAMP without affecting specificity.
    Depositor
    Justin Blau
    Article
    Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/
    Insert
    cAMPr Y205A R739A
    Expression
    Insect
    Mutation
    PKA-C Y205A, PKA-R R739A
    Promoter
    5X UAS
    Available Since
    March 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    84933-plasmid-map-sequence-id-180303

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Showing: 8601 - 8650 of 22610 results