We narrowed to 159,237 results for: Gene
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Plasmid#183069PurposeBacterial protein expression plasmid of wild-type Cas12c_4. This is a R965H version of the Cas12c in Harrington et al., 2020.DepositorInsertCas12c_4
UseCRISPRTagsHis10 and MBPExpressionBacterialMutationWild-typePromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-HemmarR2
Plasmid#112813Purposegateway cloning destination vector for expressing enhancer driven CD4-tdTomatoDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCol-TGM
Plasmid#32715DepositorTypeEmpty backboneUseMouse Targeting and RNAiTagsExpressionMutationPromoterTREAvailable sinceMay 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pMN-285
Plasmid#195472PurposeLentiviral Integratable Inducible CD19CAR using synZiFTR ZF10DepositorInsert8X ZF10 BS - ybTATA - CD19 CAR - mCherry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L1-PGK-TKNeo-L2
Plasmid#141008PurposeMutisite Gateway mediated vector constructionDepositorInsertTKNeo
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
Degron-KI p53 donor vector
Plasmid#65619PurposeDonor vector to generate Degron-KI DD-p53DepositorInsertp53 Degron-KI left and right HR arms
UseTagsExpressionMutationPromoterAvailable sinceJuly 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
CAP-AAV.FUS.3
Plasmid#222333PurposeAAV.FUS.3 Rep-Cap plasmid for production of AAV.FUS.3, a capsid with tropism for CNS neurons upon focused ultrasound mediated delivery.DepositorInsertAAV9 modified with 7-amino acid insertion between AA position 588 and 589 of VP1
UseAAVTagsExpressionMutationPromoterp41Available sinceAug. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pZmUbi-5'UTR
Plasmid#154057PurposeLevel 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Wheat construct)DepositorInsertPromoter and 5' UTR Ubiquitin (Zea mays)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterN/AAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV+ episome
Plasmid#169742PurposeCMV+ circuit on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.DepositorInserteGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterpCMV-tetO2Available sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST-HemmarG2
Plasmid#112814Purposegateway cloning destination vector for expressing enhancer driven CD4-tdGFPDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
P628_SpCas9-RecA
Plasmid#87263PurposeHuman codon optimized RecA protein was fused to SpCas9 for enhanced genome editing efficiencyDepositorInsertRecA
UseCRISPRTagsFLAGExpressionMammalianMutationnoPromoterCBhAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-DEST(f)
Plasmid#190226PurposeGateway destination vector, empty AAV vector backboneDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
M-ST1n-VP64
Plasmid#48675PurposeMammalian ST1-VP64 nuclease-null Cas9 activator expression, human optimizedDepositorInsertCas9-VP64, nuclease-null
UseCRISPRTagsNLS and VP64ExpressionMutationnuclease-null (D9A D598A H599A N622A)PromoterCMVAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCK255
Plasmid#171155PurposeExpression of I-SceI gene for genome engineering in Pseudomonas with sacB gene included for counterselection with sucrose on pBBR1-GmR plasmidDepositorInsertI-SceI_sacB
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CD9-(TEV)-tTA
Plasmid#162596Purposehuman CD9-TEV cleavage site-tTADepositorInserthuman CD9 (CD9 Human)
UseTagsTEV protease cleavage site and tTAExpressionMammalianMutationPromoterCMV promoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
PGK+ episome
Plasmid#170041PurposePGK+ circuit on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.DepositorInserteGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterPGK-tetO2Available sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_7
Plasmid#60578Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_7
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHS_235
Plasmid#195460PurposeLentiviral Integratable, 4OHT/TMX-inducible expression of synZiFTR: ZF3-p65DepositorInsertpSFFV - ZF3 - p65 - ERT2
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
PROM_MpUBE2
Plasmid#136109PurposePROM for cloning with 5UTR_MpUBE2. PROM+5UTR_MpUBE2 has ubiquitous expression in early satges of gemmae development.DepositorInsertPROM_MpUBE2 Mapoly0106s0019
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
5UTR_MpUBE2
Plasmid#136110Purpose5UTR for cloning with PROM_MpUEB2. PROM+5UTR_MpUBE2 has ubiquitous expression in early stages of gemmae development.DepositorInsert5UTR_MpUBE2 Mapoly0106s0019
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCk2_spacer
Plasmid#136072PurposeUse when making even level constructs (L2,...) if pCk2 position (position 2) has no construct assignedDepositorInsertOdd spacer
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
PROM5_35Sx2
Plasmid#136112PurposeStrong promoter that drives expression everywhere in Mp gemmae but much weaker in the notchesDepositorInsertPROM_35Sx2 (CaMV)
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
PROM5_35S
Plasmid#136111PurposeStrong promoter that drives expression everywhere in Mp gemmae but much weaker in the notchesDepositorInsertPROM_35S (CaMV)
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEMT-PT2A-iRFP670-Tdtomato-GFP
Plasmid#111817PurposeTricistronic construct: Co-express three fluorescent proteins by P2A and then T2ADepositorInsertsiRFP670
Tdtomato
GFP
UseCloning intermediateTagsExpressionMutationPromoterAvailable sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mFzd8
Plasmid#42260DepositorInsertFrizzled 8 (Fzd8 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
p426-SNR52p-gRNA.csr-1.Y-SUP4t
Plasmid#68060PurposegRNA for csr-1 locus in N. crassaDepositorInsertgRNA for csr-1 locus
UseCRISPR; N. crassa, fungiTagsExpressionYeastMutationPromoterSNR52Available sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXGFPC-5 Pxyl-dcas9 (Sth3)
Plasmid#133317Purposeexpresses dcas9 (Streptococcus thermophilus #3); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xyl locus in Caulobacter crescentus; tetracycline resistanceDepositorInsertdcas9 (Streptococcus thermophilus #3)
UseUnspecifiedTagsExpressionMutationPromoterxyloseAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVPT-GDNF-tTR-KRAB
Plasmid#11646DepositorInsertmPGK, GDNF, tTR-KRAB, Tet-on (GDNF Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO/FLAG-HA-SBP-TNRC6A
Plasmid#42044DepositorInsertTNRC6A (TNRC6A Human)
UseTagsFLAG, HA, and SBPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGGF_RT_FAST_RFP
Plasmid#136979PurposeGoldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescenceDepositorInsertseed coat marker RFP
UseTagsExpressionBacterialMutationBsaI site domestication in pOLE1:OLE1-RFP 688 G &…PromoterAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
L1_KanR-Ck1
Plasmid#136123PurposeL1 in position 1 (next to LB in L2 construct), for KanRDepositorInsertp5-MpEF1a:nptII_3t35S
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCk1_spacer
Plasmid#136071PurposeUse when making even level constructs (L2,...) if pCk1 position (position 1) has no construct assignedDepositorInsertOdd spacer
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHS-435
Plasmid#195470PurposeLentiviral Integratable Constitutive super IL-2 cytokineDepositorInsertpEF1a-Super IL-2-2A-EGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
GG-EBNA-OCT4
Plasmid#69537PurposeEpisomal plasmid encoding 5 gRNAS targeting human OCT4 promoterDepositorInsert5 concatenated gRNA transcriptional cassettes targeting OCT4
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-p65AD-GBP6
Plasmid#49440PurposeExpression of a GFP-dependent transcription factor component in mammalian cellsDepositorInsertGBP6-p65 transactivation domain fusion protein
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mFzd1
Plasmid#42253DepositorInsertFrizzled 1 (Fzd1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
AAV-hRedOpsin-mCherry
Plasmid#164426PurposeAAV plasmid expressing mCherry in photoreceptorsDepositorInsertmCherry
UseAAVTagsExpressionMammalianMutationPromoterhuman red opsinAvailable sinceNov. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMA-SpCas9-g7
Plasmid#80790PurposeBsaI-based cloning of SpCas9 gRNA guide sequence, position 7/17/27 in the arrayDepositorInsertCRISPR gRNA expression cassette (for SpCas9)
UseCRISPRTagsnaExpressionMammalianMutationPromoterU6Available sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS Galectin1
Plasmid#19216DepositorInsertGalectin1 (LGALS1 Chicken)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAC-ZETAipi
Plasmid#53284PurposeContains crtE, crtB, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and pds (crtP) gene of Synechococcus PCC7942, and thereby produces zeta-carotene in E. coliDepositorInsertpds
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterlacAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
mOtop2_pcDNA3
Plasmid#114678PurposeThis construct was used to express mouse Otop2 in HEK-293 cellsDepositorInsertOtopetrin 2 (Otop2 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_5
Plasmid#60577Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_5
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLC-241
Plasmid#73194PurposeTemplate plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (mqsR) under the control of rhamnose induceable promoter (PrhaB) for negative selection.DepositorInsertPrhaB-mqsR (mqsR )
UseTagsExpressionBacterialMutationPromoterPrhaBAvailable sinceJune 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4TO-Kif18b-24xGCN4_v1
Plasmid#72228PurposeA fusion of the Kif18b motor protein to the v1 SunTag peptide arrayDepositorInsertKif18b (KIF18B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSC6
Plasmid#104780PurposepSC218UG-At Ubiquitin10:Gateway expression cassette binary vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEE393
Plasmid#149283PurposeT-DNA encoding TRV2 with Truncated FT augmented gRNA targeting NbPDS3DepositorInsertp35S:TRV2_NbPDS3sgRNA_TrunFT:tNos
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mFzd9
Plasmid#42261DepositorInsertFrizzled 9 (Fzd9 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHS-439
Plasmid#195471PurposeLentiviral Integratable Inducible superIL-2 cytokine using synZiFTR ZF3DepositorInsert8X ZF3 BS - ybTATA - super IL-2 - 2A -EGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAG-Otx2-IRES-GFPd2
Plasmid#73996PurposePlasmid expressing Otx2(isoform b)-IRES-GFPd2DepositorInsertOtx2(isoform b)-Ires-GFP (Otx2 Mouse)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only