162,160 results

1. pCDNA3-STAT3-Y705F

   Plasmid

   #74434

   Purpose

   Expression of STAT3-Y705F

   Depositor

   Jie Chen

   Article

   Kim et al J Biol Chem. 2009 Dec 18;284(51):35425-3

   Insert

   Signal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Mutations E16K, Y705F

   Promoter

   CMV

   Available since

   May 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Ddo_piwi-1

   Plasmid

   #107628

   Purpose

   In situ hybridization and RNAi studies in planarians

   Depositor

   Alejandro Sánchez

   Article

   Accorsi et al The American Biology Teacher 79(3):208-2

   Insert

   piwi-1
   

   Use

   RNAi; In situ hybridization

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 0_T7-mScarlet3

   Plasmid

   #225927

   Purpose

   In vitro synthesis of mRNA for fluorescent protein mScarlet3 (control)

   Depositor

   Michalis Averof

   Article

   Karapidaki et al bioRxiv 2024.11.12.623055

   Insert

   mScarlet3
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSGNluc

   Plasmid

   #186705

   Purpose

   A high-sensitivity bi-directional reporter to monitor NF-κB activity in cell culture and zebrafish in real time

   Depositor

   Maria Leptin, Baubak Bajoghli

   Article

   Kuri et al J Cell Sci. 2017 Feb 1;130(3):648-657. d

   Inserts

   GFP
   luciferase
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-CMV-FLEX-SaCas9-U6-sgRosa26

   Plasmid

   #159914

   Purpose

   Mutagenesis of Rosa26 with SauCas9

   Depositor

   Larry Zweifel

   Insert

   Rosa26 gRNA (Gt(ROSA)26Sor Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAKgfplux1

   Plasmid

   #14083

   Depositor

   Attila Karsi

   Article

   Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

   Insert

   Bacterial luciferase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFUW-TetO-Fos

   Plasmid

   #131593

   Purpose

   doxycycline-inducible expression of mouse c-Fos in mammalian cells

   Depositor

   Kateri Moore, Filipe Pereira

   Article

   Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

   Insert

   FBJ osteosarcoma oncogene (Fos Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE-mCMV

   Available since

   Dec. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSCV-NLS-CBRed-d2-hygro

   Plasmid

   #139196

   Purpose

   non-ERK dependent control plasmid for pMSCV-NLS-CBGreen-FIRE-puro

   Depositor

   Matthew Lazzara

   Article

   Day et al Cell Rep. 2020 Mar 10;30(10):3383-3396.e

   Insert

   CBRed
   

   Use

   Luciferase and Retroviral

   Tags

   d2 PEST domain and nuclear localization signal (N…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDmDERusk

   Plasmid

   #213702

   Purpose

   Vector for red-light-induced bacterial gene expression

   Depositor

   Andreas Moeglich

   Article

   Meier et al Nat Commun. 2024 Jun 10;15(1):4876. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDNA3.1(+)-NEMOc

    Plasmid

    #189933

    Purpose

    Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

    Depositor

    Yubin Zhou

    Article

    Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

    Insert

    NEMOc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. mCherry-Rab5DN(S34N)

    Plasmid

    #35139

    Depositor

    Sergio Grinstein

    Article

    Bohdanowicz et al Mol Biol Cell. 2012 Jan;23(1):176-87. Ep

    Insert

    Rab5a (RAB5A Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Dominant Negative- S34N

    Promoter

    CMV

    Available since

    March 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAL119-TK

    Plasmid

    #21911

    Depositor

    Maria Castro

    Article

    Dewey et al Nat Med. 1999 Nov . 5(11):1256-63.

    Insert

    HSV Type 1 thymidine kinase
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMK33/Cas9

    Plasmid

    #133781

    Purpose

    Expresses drosophila-optimized Cas9

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Elife. 2018 Jul 27;7. pii: 36333. doi: 1

    Insert

    Drosophila-optimized Cas9
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-T7-ABE8e-SpCas9(LWKYQS)-P2A-EGFP (AHK404)

    Plasmid

    #223113

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(LWKYQS)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/LWKYQS)=D1135L, S1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-FLEX-rev-ChR2-tdtomato

    Plasmid

    #18917

    Purpose

    Cre-dependent viral expression of ChR2-tdTomato for neuronal excitation

    Depositor

    Scott Sternson

    Article

    Atasoy et al J Neurosci. 2008 Jul 9. 28(28):7025-30.

    Insert

    Channelrhodopsin 2-tdtomato
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Tags

    tdtomato

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Feb. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. HA-SFPQ

    Plasmid

    #166959

    Purpose

    Expresses HA-tagged SFPQ protein in pcDNA3.1

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    SFPQ (SFPQ Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pT3-EF1A-MYC-IRES-lucOS

    Plasmid

    #129776

    Purpose

    Transposon based vector that expresses human MYC followed by IRES and firefly luciferase and the antigens SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), and OVA323-339

    Depositor

    Amaia Lujambio

    Article

    Ruiz de Galarreta et al Cancer Discov. 2019 Jun 11. pii: 2159-82

    Insert

    human MYC (MYC Human)
    

    Use

    Tags

    OS: SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), a…

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. TFORF2998

    Plasmid

    #144474

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    GATA2 (GATA2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA-hISG15

    Plasmid

    #12447

    Depositor

    Dong-Er Zhang

    Article

    Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

    Insert

    ISG15 fragment (ISG15 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    ISG15 up to LRLRGG

    Promoter

    Available since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLentiPGK Puro DEST PKAKTRClover

    Plasmid

    #59153

    Purpose

    Lentiviral vector to express PKA KTR mClover under PGK promoter (With Puromycin Resistance)

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    PKA (PRKACA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Sept. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDEST_FLAG-HUWE1

    Plasmid

    #187120

    Purpose

    mammalian cell expression of FLAG-HUWE1

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. 5HRE/GFP

    Plasmid

    #46926

    Purpose

    hypoxia-responsive enhanced green fluorescent protein (EGFP)-based system

    Depositor

    Thomas Foster, Martin Brown

    Article

    Vordermark et al Neoplasia. 2001 Nov-Dec;3(6):527-34.

    Insert

    5X HRE of VEGF (VEGFA Human)
    

    Use

    Tags

    d2EGFP

    Expression

    Mammalian

    Mutation

    five copies of a 35-bp fragment from the hypoxia-…

    Promoter

    EF-1α promoter

    Available since

    Aug. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLVX-IRES-Hygro_GCaMP6s

    Plasmid

    #164591

    Purpose

    Stable integration and expression of GCaMP6s in mammalian cells

    Depositor

    Joseph Hyser

    Article

    Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

    Insert

    GCaMP6s
    

    Use

    Lentiviral

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. HA-NFAT1(4-460)-GFP

    Plasmid

    #11107

    Purpose

    Expresses mouse NFAT1 (amino acids 4-460) with HA and GFP tags in mammalian cells

    Depositor

    Anjana Rao

    Article

    Aramburu et al Science. 1999 Sep 24. 285(5436):2129-33.

    Insert

    NFAT1 (Nfatc2 Mouse)
    

    Use

    Tags

    3x HA and GFP

    Expression

    Mammalian

    Mutation

    Contains amino acids 4-460 of NFAT1; L78P compare…

    Promoter

    CMV

    Available since

    May 31, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. ER-HA-mNG21-10

    Plasmid

    #141165

    Purpose

    Split Fluorescent Reporter for ER Retrograde Trafficking

    Depositor

    Wayne Lencer

    Article

    Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

    Insert

    ER-HA-mNG21-10-KDEL
    

    Use

    Lentiviral

    Tags

    Signal Sequence, KDEL and HA

    Expression

    Mammalian

    Mutation

    K128M, S142T, R150M, G172V and K213M

    Promoter

    Ef1a

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pACYC-TtCas6-4xcrRNA4.5

    Plasmid

    #127764

    Purpose

    For expression of T. thermophilus Csm complex in E. coli (crRNA)

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nat Commun. 2019 Jul 5;10(1):3001. doi:

    Inserts

    Synthetic CRISPR array containing four identical T. thermophilus spacers
    Cas6A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for expression in E. coli

    Promoter

    T7

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-hSyn-miniDi-P2A-mCherry

    Plasmid

    #204358

    Purpose

    AAV vector for miniDi expression under the control of human synapsin promoter

    Depositor

    Masao Doi

    Article

    Miyake et al Cell Rep Methods. 2024 Oct 21;4(10):1008

    Insert

    miniDi-P2A-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    The third intracellular loop (ICL3) of hM4Di was …

    Promoter

    hSyn

    Available since

    Oct. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDmREDusk

    Plasmid

    #213701

    Purpose

    Vector for red-light-repressed bacterial gene expression

    Depositor

    Andreas Moeglich

    Article

    Meier et al Nat Commun. 2024 Jun 10;15(1):4876. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAP05

    Plasmid

    #102920

    Purpose

    Direct cloning and broad-host-range heterologous expression vector

    Depositor

    Bradley Moore

    Article

    Zhang et al MBio. 2017 Sep 5;8(5). pii: e01291-17. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. FUGW-ER-TurboID

    Plasmid

    #200965

    Purpose

    3rd gen lentiviral plasmid for expressing endoplasmic reticulum localized TurboID with EGFP

    Depositor

    Keriann Backus

    Article

    Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

    Insert

    ER-TurboID
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Flag-mCherry-Binder

    Plasmid

    #179139

    Purpose

    Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9). Binder binds to the 7 a.a. peptide SsrA (AANDENY).

    Depositor

    Klaus Hahn

    Article

    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

    Insert

    SspB
    

    Use

    Tags

    FLAG and mCherry

    Expression

    Mammalian

    Mutation

    Y11K/A15E, residue numbering from pdb: 1ou9

    Promoter

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40

    Plasmid

    #124603

    Purpose

    Cre-dependent soma-localized ChrimsonR for optogenetic stimulation

    Depositor

    Christopher Harvey

    Article

    Chettih et al Nature. 2019 Mar 6. pii: 10.1038/s41586-

    Has Service

    AAV9

    Insert

    Soma-localized ChrimsonR
    

    Use

    AAV

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAVone-AAV2-CMV-eGFP

    Plasmid

    #230930

    Purpose

    pAAVone plasmid is used to package AAV-CMV-eGFP with an AAV2 serotype, utilizing the AAVone single-plasmid AAV production system.

    Depositor

    Qizhao Wang

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. DH-BB(5'SIN;TE12ORF)

    Plasmid

    #72309

    Purpose

    Defective Helper to produce neurotropic, propagation-incompetent Sindbis virus

    Depositor

    Anthony Zador

    Insert

    DH-BB(5'SIN;TE12ORF)
    

    Use

    Sindbis virus helper construct

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. v3em-Cterm-PE6d-dualU6-Rosa26-twinPE-attB

    Plasmid

    #207860

    Purpose

    AAV genome encoding C-terminal PE6d and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    NpuC-Cterm-PE6d-dualU6-Rosa26-twinPE-attB
    

    Use

    AAV

    Tags

    Expression

    Mutation

    See Manuscript

    Promoter

    Cbh

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. CROPSeq-BFP

    Plasmid

    #196718

    Purpose

    CROP-Seq library plasmid with Chen tracrRNA (PMID: 24360272) and TagBFP

    Depositor

    Bernhard Schmierer

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. TMPRSS2

    Plasmid

    #53887

    Purpose

    Expression of TMPRSS2

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Has Service

    Cloning Grade DNA

    Insert

    TMPRSS2 (TMPRSS2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pLX301

    Plasmid

    #25895

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. YopH (residues 164-468)

    Plasmid

    #79749

    Purpose

    This plasmid encodes the active catalytic domain (amino acid residues 164-468) of YopH. Inteded for co-expression with human Tyr kinase plasmids to enhance bacterial kinase expression.

    Depositor

    Markus Seeliger, John Chodera, Nicholas Levinson

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    YopH (residues 164-468)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pWN_U6-TRAC-miniGag

    Plasmid

    #228960

    Purpose

    miniEDV production plasmid. Expresses the miniGag polypeptide (CAG promoter) and sgRNA (human U6 promoter)

    Depositor

    Jennifer Doudna

    Article

    Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

    Insert

    miniGag
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge