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179,521 results

Showing: 7041 - 7080 of 179521 results

  1. pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA

    Plasmid
    #154868
    Purpose
    Human synapsin-1 promoter; Flp-dependent hM3D(Gq) DREADD-mCherry
    Depositor
    Ulrik Gether
    Has service
    AAV8 and AAV Retrograde
    Insert
    hM3D(Gq)-mCherry
    Use
    AAV; Flp-frt
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    Human Synapsin-1
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    154868-plasmid-map-sequence-id-308827

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  2. p3xFLAG SARS-CoV2 RdRp.co

    Plasmid
    #154008
    Purpose
    Expression of codon-optimized SARS-CoV2 Nsp12 (RdRp) in mammalian cells
    Depositor
    Patrick Moore
    Publication
    Meng et al J Med Virol. 2023 Jan;95(1):e28246. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Codon-optimized SARS-CoV2 Nsp12 from ORF1ab (ORF1ab Synthetic)
    Tags
    3xFLAG tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 23, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154008-plasmid-map-sequence-id-300786

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  5. pCAG-All-in-one-hCascade

    Plasmid
    #134919
    Purpose
    Components for genome editing in mammalian cells with pPB-CAG-hCas3 and pBS-U6-crRNA-targeted. This plasmid coexpresses PuroR protein.
    Depositor
    Tomoji Mashimo
    Publication
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cascade factors (Cse1(Cas8), Cse2(Cas11), Cas5, Cas6, Cas7) with bpNLS
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Feb. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    134919-plasmid-map-sequence-id-272938

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  6. MLRV

    Plasmid
    #118069
    Purpose
    Multi-luciferase reporter vector including transcriptional reporters for NF-kb, TGF-b, c-Myc, p53 and MAPK/JNK transcriptional reporters
    Depositor
    Koen Venken
    Publication
    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    TB:5xNF-κβ:RedF:bGHpA
    TB:4xTGF-β:FLuc:bGHpA
    TB:5xE-box:Renilla:bGHpA
    TB:2xp53:NLuc:bGHpA
    TB:6xAP-1:GrRenilla:bGHpA
    CMV:ELuc:bGHpA
    Use
    Luciferase and Synthetic Biology; Assembly of gb2…
    Expression
    Mammalian
    Promoter
    2xp53::miniP, 4xTGF-B::miniP, 5xEbox::miniP, 5xNF…
    Available since
    Dec. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    118069-plasmid-map-sequence-id-240503

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  13. CDH5-AbR-GFP

    Plasmid
    #122970
    Purpose
    Lenti plasmid containing a CDH5 promoter driving GFP followed by T2A and an antibiotic resistance cassette (zeocin). Used for selection of endothelial cells.
    Depositor
    Budd A. Tucker
    Inserts
    CDH5p
    CopGFP
    Zeocin
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    NA
    Available since
    May 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    122970-plasmid-map-sequence-id-244602

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  19. pAAV-SCP1-dSa VPR mini.-2X snRP-1 BsaI gRNA

    Plasmid
    #99698
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA with BsaI cloning sites for programming, vector allows for strong activation of programmed target gene, can be packaged and delivered as AAV
    Depositor
    George Church
    Type
    Empty backbone
    Use
    AAV
    Available since
    Aug. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    99698-plasmid-map-sequence-id-194602

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  20. pOPINE GFP nanobody:Halo:His6

    Plasmid
    #111090
    Purpose
    Bacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.
    Depositor
    Lennart Wirthmueller
    Publication
    Chen et al Front Plant Sci. 2018 Nov 1;9:1581. doi:
    Insert
    GFP Nanobody
    Tags
    Halo and His6
    Expression
    Bacterial, Insect, and Mamm…
    Promoter
    T7 promoter
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    111090-plasmid-map-sequence-id-264731

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  21. VEGFR2-mCh

    Plasmid
    #108854
    Purpose
    Encodes for human VEGFR2 fluorescently labeled with mCherry on the C-Terminus via a 3 amino acid (GGS) flexible linker
    Depositor
    Kalina Hristova
    Publication
    Sarabipour et al Elife. 2016 Apr 7;5:e13876. doi: 10.7554
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KDR (KDR Human)
    Tags
    Labeled with mCherry on the C-Terminus via a 3 am…
    Expression
    Mammalian
    Available since
    May 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    108854-plasmid-map-sequence-id-208010

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  25. pQUAST-attB

    Plasmid
    #104880
    Purpose
    For insertion of 5xQUAS-geneX transgenes using PhiC31 integrase system
    Depositor
    Christopher Potter
    Type
    Empty backbone
    Expression
    Insect
    Available since
    March 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    104880-plasmid-map-sequence-id-204757

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  26. pAAV.CAG.GCaMP6s.WPRE.SV40

    Plasmid
    #100844
    Purpose
    AAV expression of ultrasensitive protein calcium sensor from CAG promoter
    Depositor
    Douglas Kim, GENIE Project
    Publication
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Has service
    AAV1, AAV5, and AAV9
    Insert
    GCaMP6s
    Use
    AAV
    Expression
    Mammalian
    Mutation
    GCaMP3-K78H T302L R303P D380Y T381R S383T R392G
    Promoter
    CAG
    Available since
    Feb. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100844-plasmid-map-sequence-id-461658

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  30. pThermoCas9_ctrl

    Plasmid
    #100981
    Purpose
    Expresses ThermoCas9 and its sgRNA module
    Depositor
    John van der Oost
    Publication
    Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:
    Inserts
    Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
    ThermoCas9 single guide RNA expressing module
    Tags
    None
    Expression
    Bacterial
    Promoter
    B. coagulans DSM 1 pta promoter and B. smithii xy…
    Available since
    Nov. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100981-plasmid-map-sequence-id-196605

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  32. pSupAR-MbPylRS(DiZPK)

    Plasmid
    #91705
    Purpose
    express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interest
    Depositor
    P. Chen
    Publication
    Zhang et al Nat Chem Biol. 2011 Sep 4;7(10):671-7. d
    Inserts
    pylS
    pylT
    Expression
    Bacterial
    Mutation
    L274A, C313S
    Available since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    91705-plasmid-map-sequence-id-183278

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  37. InsRA-eGFP

    Plasmid
    #79795
    Purpose
    Insulin receptor isoform A with a inter-domain eGFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.
    Depositor
    James Johnson
    Publication
    Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    InsRA (INSR Human)
    Tags
    eGFP extracellular
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    79795-plasmid-map-sequence-id-149406

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Showing: 7041 - 7080 of 179521 results