159,557 results

1. pHR-PGK-PYL1-sfGFP-Coilin

   Plasmid

   #122177

   Purpose

   expression of PYL1-SfGFP-Coilin, lentivirus vector

   Depositor

   Stanley Qi

   Article

   Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

   Insert

   Coilin (COIL Human)
   

   Use

   Lentiviral

   Tags

   PYL1-SFGFP

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   April 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmScarlet_C1

   Plasmid

   #85042

   Purpose

   Mammalian expression of mScarlet

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Has Service

   Cloning Grade DNA

   Insert

   mScarlet
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMM553

   Plasmid

   #68543

   Purpose

   NanoLuc expressed constitutively from PBT1311, pNBU2 backbone, AmpR

   Depositor

   Timothy Lu, Christopher Voigt

   Article

   Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

   Insert

   NanoLuc
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-TA-ERN

   Plasmid

   #80474

   Purpose

   All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and neomycin resistance)

   Depositor

   Knut Woltjen

   Article

   Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

   Type

   Empty backbone

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. Pet28a_Human_H2B.1

   Plasmid

   #42630

   Depositor

   Joe Landry

   Insert

   Human H2B.1 (H2BC8 Human)
   

   Use

   Tags

   Untagged

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 22, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pcDNA6-N-3XFLAG-Ctnnb1

   Plasmid

   #123586

   Depositor

   Bruce Beutler

   Article

   Choi et al Science. 2019 May 10;364(6440). pii: 364

   Insert

   catenin (cadherin associated protein), beta 1 (Ctnnb1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC57-TPNOX

   Plasmid

   #87853

   Purpose

   This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.

   Depositor

   Vamsi Mootha

   Article

   Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/

   Insert

   TPNOX
   

   Use

   Tags

   C-terminal FLAG tag with a linker

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF0424

   Plasmid

   #142262

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PBX3 (PBX3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pES001 MBP-GSK3β-HA-His

   Plasmid

   #154010

   Purpose

   Expresses MBP-GSK3β-HA-His (human Glycogen Synthase Kinase 3β as a fusion protein with MBP, HA, and His- tags) in E.coli

   Depositor

   Jesse Zalatan

   Article

   Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

   Insert

   GSK3B (GSK3B Human)
   

   Use

   Tags

   HA tag, His6, and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLPC-JAG1-mVenus

    Plasmid

    #171170

    Purpose

    Retroviral vector for CMV promoter driven expression of JAG1 (Notch pathway) fused to mVenus fluorescent protein (to be used in conjunction with Phoenix packaging cells).

    Depositor

    Masashi Narita

    Article

    Hoare et al Nat Cell Biol. 2016 Sep;18(9):979-92. do

    Insert

    JAG1 (JAG1 Human)
    

    Use

    Retroviral

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBb(RSF1010)1k-GFPuv

    Plasmid

    #106395

    Purpose

    IPTG-inducible trc promoter expressing gfpuv on RSF1010 origin

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    GFPuv
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV_BiSSTe4_ChR2_mCherry

    Plasmid

    #213945

    Purpose

    AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by SST interneuron-targeting enhancer. Alias: pAAV_WDS0004_ChR2_mCherry

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Has Service

    AAV PHP.eB

    Insert

    Channelrhodopsin 2-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNatB (pACYCduet-naa20-naa25)

    Plasmid

    #53613

    Purpose

    Allows expression of the fission yeast NatB complex - chloramphenicol marker

    Depositor

    Dan Mulvihill

    Article

    Johnson et al PLoS One. 2010 Dec 23;5(12):e15801. doi:

    Inserts

    naa20 (naa20 Fission Yeast)
    naa25 (naa25 Fission Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pU6-PspCas13b-gRNA-Actb1216

    Plasmid

    #155368

    Purpose

    PspCas13b guide RNA positioned 8bp 5' of Actb 1216 for targeted m6A RNA methylation

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    PspCas13b gRNA targeting Actb 1216
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. eGFPC1 FKBP

    Plasmid

    #67529

    Purpose

    Expresses GFP fused FKBP

    Depositor

    Stephen Tait

    Article

    Ichim et al Mol Cell. 2015 Mar 5;57(5):860-72. doi:

    Insert

    FKBP
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    F36V

    Promoter

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR_PGK_SNIPR_hsNotch1 LNR, Notch2 JMD

    Plasmid

    #188374

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch1-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_Notch1-ECD_Notch1-TMD_Notch2-JMD_Gal4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEJS2437_Nme2.Smu-ABE8e-nt

    Plasmid

    #201519

    Purpose

    Mammalian expression of N-terminally fused Nme2.Smu-ABE8e-nt

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

    Insert

    Nme2.Smu-ABE8e-nt
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. CoV2-M-IRES-E

    Plasmid

    #177938

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence) (E, M )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. p206-Switch-ON

    Plasmid

    #217885

    Purpose

    Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

    Depositor

    Ulrich Elling

    Article

    Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TPC1-G-GECO1.2

    Plasmid

    #207143

    Purpose

    Fusion of Ca2+ reporter and endosomal Ca2+-permeable channel

    Depositor

    Antony Galione

    Article

    Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

    Insert

    TPCN1 (TPCN1 Human)
    

    Use

    Tags

    G-GECO1.2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pWZL hygro 12S E1A

    Plasmid

    #18748

    Depositor

    Scott Lowe

    Article

    Serrano et al Cell. 1997 Mar 7. 88(5):593-602.

    Insert

    12S E1A
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRPN265NB-enh-MNase

    Plasmid

    #166035

    Purpose

    Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

    Depositor

    Marc Timmers

    Article

    Nizamuddin et al Nucleic Acids Res. 2021 May 21;49(9):e49

    Insert

    Glutathione-S-Transferase/enhancer/MNase
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    lac promoter / tac promoter

    Available since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCS2+ centrin-mKate2

    Plasmid

    #105941

    Purpose

    in vitro transcription, zf centrin N-terminally tagged with mkate2

    Depositor

    Caren Norden

    Article

    Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

    Insert

    Centrin
    

    Use

    Tags

    mKate2

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pVC-FUS-WT

    Plasmid

    #175066

    Purpose

    expresses mVenus and mCherry tagged to FUS wildtype in mammalian cells

    Depositor

    Arnold Boersma

    Article

    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

    Insert

    FUS glycine rich protein (FUS Human)
    

    Use

    Tags

    mVenus + mCherry

    Expression

    Mammalian

    Mutation

    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…

    Promoter

    CMV Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pegRNA-GFP>BFP_Y66H-NGG

    Plasmid

    #185480

    Purpose

    pegRNA plasmid in order to make a Y66H (TAC>CAT) conversion in the GFP gene, creating a BFP gene.

    Depositor

    Sabine Fuchs

    Article

    Schene et al Nat Commun. 2022 Mar 1;13(1):1028. doi:

    Insert

    GFP>BFP_Y66H-pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. HRE-doUnaG

    Plasmid

    #201713

    Purpose

    Hypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1a and a PEST sequence

    Depositor

    Friedemann Kiefer

    Article

    Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

    Insert

    UnaG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEERM3 Km

    Plasmid

    #64025

    Purpose

    Plasmid for integration into neutral site of Synechocystis 6803

    Depositor

    Pia Lindberg

    Article

    Englund et al ACS Synth Biol. 2015 Jul 13.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PnrsB

    Available since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Sniper2L

    Plasmid

    #193856

    Purpose

    Expresses human codon-optimized Sniper2L and blasticidin resistance: EFS promoter-Sniper2L-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Chem Biol. 2023 Mar 9. doi: 10.1038/

    Insert

    Sniper2L
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    E1007L

    Promoter

    EFS

    Available since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTECH-chAcK3RS(IPYE)

    Plasmid

    #104069

    Purpose

    Expression plasmid encoding chAcK3RS(IPYE) and pylT

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Plpp chAcK3RS(IPYE); PproK pyl
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains activating mutations V31I, T56P, H62Y, A…

    Promoter

    Plpp and PproK

    Available since

    Dec. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLX304-Flag-APEX2-NES

    Plasmid

    #92158

    Purpose

    Cytosolic APEX2 construct

    Depositor

    Alice Ting

    Article

    Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

    Insert

    Flag-APEX2-NES
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pUCBB-eGFP

    Plasmid

    #32548

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    eGFP (eGFP )
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Consitutive lac

    Available since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET-28a(+)::NL

    Plasmid

    #141289

    Purpose

    NLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

    Depositor

    Andrew Millar

    Article

    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

    Insert

    NLUC
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Engineered for high stability (t1/2 = 11.5 days a…

    Promoter

    promoter for bacteriophage T7 RNA polymerase

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. hCMV-IE1:Renilla

    Plasmid

    #118066

    Purpose

    Transcriptional unit for constitutive expression of Renilla in mammalian cells

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    CMV:GreenRenilla:bGHF
    

    Use

    Luciferase and Synthetic Biology; Assembly of gb2…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. GFP-C1-CAMKIIalpha

    Plasmid

    #21226

    Depositor

    Tobias Meyer

    Article

    Shen et al Neuron. 1998 Sep . 21(3):593-606.

    Insert

    CAMKIIalpha (Camk2a Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Lab plasmid KS 0001.

    Promoter

    Available since

    July 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. acGFP WC visual barcode

    Plasmid

    #158672

    Purpose

    tagging clonal populations with whole cell acGFP fluorescent protein

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    acGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. hSyn1-eYFP

    Plasmid

    #117382

    Purpose

    An AAV genome that expresses the fluorescent protein eYFP from the hSyn1 promoter

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV2, AAV5, AAV8, and AAV9

    Insert

    EYFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn1

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET22b-csgA

    Plasmid

    #89581

    Purpose

    Express csgA curli protein with GS linker and Histaq

    Depositor

    Urartu Seker

    Article

    Abdelwahab et al Langmuir. 2017 Apr 7. doi: 10.1021/acs.l

    Insert

    csgA (csgA )
    

    Use

    Tags

    3xGGSG linker - TEV site - 3xGGSG linker and 6xHi…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pVP143

    Plasmid

    #222080

    Purpose

    Single component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion and AeBlue for plasmid eviction.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al Biodes Res. 2025 March, 7:100001. doi: 1

    Insert

    dCas9-PmCDA1, sfGFP, sacB and AeBlue
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. TFORF3343

    Plasmid

    #144819

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    RARB (RARB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pJL1-CRM197-4xDQNAT

    Plasmid

    #128395

    Purpose

    In vitro expression of CRM197 with C terminal 4xDQNAT glycosylation sites

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Diphtheria toxin
    

    Use

    Tags

    4xDQNAT glycosylation tag and 6xHis tag

    Expression

    Bacterial

    Mutation

    Inactivating G52E mutation

    Promoter

    T7

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge