166,027 results

1. pmCherry-ATG5

   Plasmid

   #13095

   Depositor

   Roberta Gottlieb

   Article

   Hamacher-Brady et al Cell Death Differ. 2007 Jan;14(1):146-57

   Insert

   ATG5 (Atg5 Mouse)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   Nov. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. opto-Cdc42

   Plasmid

   #174510

   Purpose

   Expresses opto-Cdc42 (mCherry-GGGSx2-Cdc42-GGGSx2-BcLOV4-GGGS-3xFLAG) in a pcDNA3.1 backbone.

   Depositor

   Brian Chow

   Article

   Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

   Insert

   opto-Cdc42-mCherry
   

   Tags

   3xFLAG and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3- sgAAVS1.5-CAG-Cas9-T2A-mCherry-P2A-Puro

   Plasmid

   #216246

   Purpose

   Express Cas9 with CAG promoter to improve the expression in hES cells and a highly efficient sgAAVS1.5 for AAVS1 integration

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   Cas9 and sgAAVS1.5
   

   Use

   CRISPR; Human safe harbor locus (a avs1) site-spe…

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMV-LUC2CP/ARE

   Plasmid

   #62857

   Purpose

   Splicing reporter control (no intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.

   Depositor

   Gideon Dreyfuss

   Article

   Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d

   Insert

   Luciferase
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   destabilizing sequences (DS) added to the C termi…

   Promoter

   CMV

   Available Since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBABE-puro-GFP-progerin

   Plasmid

   #17663

   Depositor

   Tom Misteli

   Article

   Scaffidi et al Nat Cell Biol. 2008 Apr;10(4):452-9. doi

   Insert

   GFP-D50 lamin A (LMNA Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Delta 50

   Available Since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pICE-EGFP-FLAG-PLIN3

   Plasmid

   #161918

   Purpose

   Expresses human PLIN3 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells.

   Depositor

   Sébastien Britton

   Article

   Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

   Insert

   Perilipin-3 (PLIN3 Human)
   

   Tags

   GFP-FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-TRE-fDIO-GFP-IRES-tTA

   Plasmid

   #118026

   Purpose

   AAV vector for sparse and bright labeling in a cell-type specific manner

   Depositor

   Minmin Luo

   Article

   Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

   Insert

   TRE-fDIO-GFP-IRES-tTA
   

   Use

   AAV

   Mutation

   See depositor comments below

   Promoter

   TRE

   Available Since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX458-Ef1a-dCas9-VPR-H2B-GFP (CRISPRa)

   Plasmid

   #175571

   Purpose

   Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation

   Depositor

   Joseph Brzezinski

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. YAP/TAZ tdTomato TEAD Binding Reporter

   Plasmid

   #228554

   Purpose

   Encodes a tdTomato fluorescent protein gene driven by a TEAD binding synthetic promoter. Used to track YAP/TAZ signaling pathway activity.

   Depositor

   Cole DeForest

   Article

   Kopyeva et al Adv Mater. 2024 Sep 6:e2404880. doi: 10.

   Insert

   tdTomato
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   GTIIC TEAD-Binding Responsive Element

   Available Since

   Feb. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOPTN-EGFP

    Plasmid

    #27052

    Depositor

    Beatrice Yue

    Article

    Park et al Am J Pathol. 2006 Dec . 169(6):1976-89.

    Insert

    optineurin (OPTN Human)
    

    Expression

    Mammalian

    Available Since

    March 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-Rab4B

    Plasmid

    #61801

    Purpose

    Human Rab4B fluorescently tagged with GFP on the N-terminus for mammalian expression

    Depositor

    Gia Voeltz

    Article

    Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

    Insert

    Rab4B (RAB4B Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB533_2E12LI-sfGFP

    Plasmid

    #167527

    Purpose

    H3K27me3-mintbody expression vector for mammalian cells

    Depositor

    Hiroshi Kimura

    Article

    Tjalsma et al EMBO Rep. 2021 Feb 19:e51989. doi: 10.15

    Insert

    2E12LI-scFv
    

    Tags

    sfGFP

    Expression

    Mammalian

    Available Since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBbS8k-RFP

    Plasmid

    #35276

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    BAD

    Available Since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG mito-GCaMP5G

    Plasmid

    #105009

    Purpose

    mitochondrial calcium sensor

    Depositor

    Franck Polleux

    Article

    Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

    Insert

    GCaMP5G
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. CAG FUCCI

    Plasmid

    #175266

    Purpose

    Bicistronic FUCCI construct driven by a CAG promoter. Contains I-SceI sites for transgenesis

    Depositor

    James Monaghan

    Article

    Duerr et al Development. 2022 Mar 15;149(6). pii: 27

    Insert

    FUCCI
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn-HA-hM3D(Gq)-IRES-mCitrine

    Plasmid

    #50463

    Purpose

    Gq-coupled hM3D-IRES-mCitrine under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Insert

    hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
    

    Use

    AAV

    Tags

    HA

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    human Synapsin 1

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. PresentER-SIINFEKL (mCherry)

    Plasmid

    #102945

    Purpose

    PresentER minigene encoding H2-Kb ligand SIINFEKL (mCherry)

    Depositor

    David Scheinberg

    Article

    Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

    Insert

    SIINFEKL (OVAL Synthetic, Chicken)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    OVA257-264

    Promoter

    LTR

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

18. pCold-I-Dsup

    Plasmid

    #90021

    Purpose

    To express His-tagged Dsup in bacteria

    Depositor

    Takekazu Kunieda

    Article

    Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

    Insert

    Dsup
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    cspA

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL3-EF1a-i53

    Plasmid

    #216243

    Purpose

    Express i53 in mammalian cells as a HDR enhancer

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    i53
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGWB551

    Plasmid

    #74881

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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