163,607 results

1. pCMV-evoCAST-eCas8

   Plasmid

   #234727

   Purpose

   Engineered human-codon optimized PseCas8 in mammalian cells (for evoCAST)

   Depositor

   David Liu

   Article

   Witte et al Science. 2025 May 15;388(6748):eadt5199.

   Insert

   ePseCas8
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTargetF

   Plasmid

   #62226

   Purpose

   Constitutive expression of sgRNA without donor editing template DNA

   Depositor

   Sheng Yang

   Article

   Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

   Insert

   sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pij23119

   Available Since

   March 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330-U6-Chimeric_BB-CBh-hSpCas9

   Plasmid

   #42230

   Purpose

   A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Has Service

   Cloning Grade DNA

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDGB3_alpha1-pAtUBQ10:Myc-GVG-HA-tE9-6xUAS-35Smini-HA-LRR-NEL-mcherry-Tnos

   Plasmid

   #211842

   Purpose

   Plant transient expression cassette for a Dexamethasone-inducible E3-DART ligase

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Inserts

   LRR-NEL
   GAL4-VP16-GR
   

   Use

   Synthetic Biology

   Tags

   HA, Myc, and mCherry

   Expression

   Plant

   Mutation

   Promoter

   6xUAS-35Smini and pUBQ10

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTU2-AmyE-Pveg-eGFP

   Plasmid

   #112792

   Purpose

   Expresses GFP under the control of Pveg promoter and it is integrated into AmyE locus. Used to test correct transformation

   Depositor

   Chris French

   Article

   Wicke et al bioRxiv 185108

   Insert

   Pveg-eGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMV-Mito4x-GCaMP6f

   Plasmid

   #127870

   Purpose

   Fluorescent reporter for mitochondrial matrix Ca2+

   Depositor

   Timothy Ryan

   Article

   Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

   Insert

   4 repeats of COX8 signal peptide plus GCaMP6f
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-Bxb1

   Plasmid

   #182142

   Purpose

   Expression of human codon-optimized Bxb1

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   human codon optimized Bxb1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-C1-G3BP1-WT

   Plasmid

   #135997

   Purpose

   WT G3BP1 inserted with GFP tagged on the N-terminus used to stably integrate into cells

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   G3BP1 (G3BP1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. PGKp-GFP-YAP

   Plasmid

   #174172

   Purpose

   Lentiviral vector to constitutively express YAP under control of the human PGK promoter.

   Depositor

   Rod Bremner

   Article

   Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

   Insert

   YAP (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   3x FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   Aug. 19, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. TETv4

    Plasmid

    #167983

    Purpose

    Expresses TETv4 (TET1CD-XTEN80-dCas9-3xNLS-P2A-BFP) downstream of the CAG promoter

    Depositor

    Luke Gilbert

    Article

    Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003

    Insert

    TETv4 (TET1CD-XTEN80-dCas9)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    3xNLS, TET1 catalytic domain, and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO.1 - TRC cloning vector

    Plasmid

    #10878

    Purpose

    3rd generation transfer plasmid

    Depositor

    David Root

    Article

    Moffat et al Cell. 2006 Mar 24. 124(6):1283-98.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDestTol2CG2--3.5ubb:plin2-tdtomato

    Plasmid

    #172534

    Purpose

    Zebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with cmlc2:eGFP as a transgenesis marker

    Depositor

    Richard White

    Article

    Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

    Insert

    -3.5ubb:plin2-tdtomato
    

    Use

    Tags

    Expression

    Mutation

    S80R, A81S, E144D, I148F and R415H mutations obse…

    Promoter

    zebrafish ubb

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Arl13b-mcherry

    Plasmid

    #234845

    Purpose

    A mammalian expression plasmid encoding Arl13b with mcherry at C-terminus.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Arl13b (ARL13B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. actc1b-mKate2-P2A-APEX2-VHH[GFP]

    Plasmid

    #126749

    Purpose

    Expression of GFP binding nanobody fused to APEX2 in zebrafish muscle. Parton lab clone DSJ

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    VHH[EGFP]
    

    Use

    Zebrafish muscle expression clone

    Tags

    mKate2-P2A-APEX2

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-pHluorin_M153R-CD63-mScarlet

    Plasmid

    #172118

    Purpose

    Dual-color fluorescent reporter for CD63-positive exosome secretion and uptake

    Depositor

    Alissa Weaver

    Article

    Sung et al Nat Commun. 2020 Apr 29;11(1):2092. doi:

    Insert

    CD63 (CD63 Human)
    

    Use

    Lentiviral

    Tags

    mScarlet and pHluorin_M153R in a small extracellu…

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCW57.1-MAT2A

    Plasmid

    #100521

    Purpose

    Dox-repressible cDNA expression of MAT2A (dox-off/ tet-off all-in-one vector)

    Depositor

    David Sabatini

    Article

    Gu et al Science. 2017 Nov 10;358(6364):813-818.

    Insert

    MAT2A (MAT2A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTight TRE

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330S-2-PITCh

    Plasmid

    #63670

    Purpose

    Expresses Cas9 nuclease and the PITCh-gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-PE7-P2A-BSD

    Plasmid

    #224122

    Purpose

    Mammalian expression of SpCas9 PE7 prime editor with P2A-BSD marker

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7-P2A-BSD
    

    Use

    Tags

    P2A-BSD, SV40 bpNLS, and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. Tet-pLKO-neo

    Plasmid

    #21916

    Purpose

    3rd generation lentiviral plasmid for inducible expression of shRNA; neomycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.

    Depositor

    Dmitri Wiederschain

    Article

    Wiederschain et al Cell Cycle. 2009 Feb 1. 8(3):498-504.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAVS1_TREtight-GEM2-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid

    #197061

    Purpose

    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM2-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Inserts

    Encapsulin
    Adaptor Protein
    

    Use

    CRISPR

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Promoter

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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