4,200 results

1. AAV-CMV-GFP

   Plasmid

   #67634

   Purpose

   AAV vector expressing GFP

   Depositor

   Connie Cepko

   Article

   Xiong et al J Clin Invest. 2015 Apr;125(4):1433-45.

   Insert

   GFP
   

   Use

   AAV

   Promoter

   CMV

   Available Since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCXLE-hOCT3/4-shp53-F

   Plasmid

   #27077

   Purpose

   Integration-free (episomal) expression of human OCT3/4 and shRNA against p53

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Nat Methods. 2011 May;8(5):409-12

   Insert

   OCT3/4 (POU5F1 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. mVenus N1

   Plasmid

   #27793

   Depositor

   Steven Vogel

   Article

   Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

   Has Service

   Cloning Grade DNA

   Insert

   mVenus N1
   

   Expression

   Mammalian

   Available Since

   April 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. dCas9-hHDAC3

   Plasmid

   #98591

   Purpose

   3rd generation lenti vector encoding dCas9-hHDAC3 (EF1a-NLS-dCas9(N863)-hHDAC3). Expresses dCas9 fused to human HDAC3.

   Depositor

   Zhaolan Zhou

   Article

   Kwon et al Nat Commun. 2017 May 12;8:15315. doi: 10

   Insert

   dCAS9-HDAC3 (HDAC3 Human, Synthetic, S. pyogenes)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   EF1A

   Available Since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-MMLVgag-3xNES-ABE8e

   Plasmid

   #181751

   Purpose

   MMLV-Gag fused to 3xNES-ABE8e with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

   Depositor

   David Liu

   Article

   Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

   Insert

   MMLVgag-3xNES-ABE8e
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO5.sgRNA.EFS.tRFP

   Plasmid

   #57823

   Purpose

   3rd generation Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP, EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   sgRNA
   EFS
   tagRFP
   

   Use

   CRISPR and Lentiviral

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-CFP

   Plasmid

   #13030

   Purpose

   Mammalian expression vector for cloning your gene fused to CFP

   Depositor

   Doug Golenbock

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Nov. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXs-puro GFP-p62

   Plasmid

   #38277

   Depositor

   Noboru Mizushima

   Article

   Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

   Insert

   Sequestosome-1 (Sqstm1 Mouse)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Aug. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCXLE-hUL

   Plasmid

   #27080

   Purpose

   Integration-free (episomal) expression of human L-MYC and LIN28

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Nat Methods. 2011 May;8(5):409-12

   Insert

   L-MYC (LIN28A, MYCL Human)
   

   Expression

   Mammalian

   Available Since

   March 10, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-EF1a-IRES-Neo

    Plasmid

    #85139

    Purpose

    lentiviral backbone

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. PKD1-F (pCI-PKD1-Flag)

    Plasmid

    #21369

    Depositor

    Gregory Germino

    Article

    Hanaoka et al Nature. . 408(6815):990-4.

    Insert

    autosomal dominant polycystic kidney disease type I (PKD1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    wild-type sequence*

    Available Since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGreenIIM DR5v2-ntdTomato/DR5-n3GFP

    Plasmid

    #61628

    Purpose

    Expresses nuclear tdTomato from auxin-dependent DR5v2 (TGTCGG) promoter and nuclear 3xGFP from the DR5 (TGTCTC) promoter

    Depositor

    Dolf Weijers

    Article

    Liao et al Nat Methods. 2015 Mar;12(3):207-10. doi:

    Inserts

    ntdTomato
    n2xEGFP
    

    Expression

    Plant

    Mutation

    2 copies of EGFP with N-terminal SV40 NLS

    Promoter

    Auxin-dependent DR5 promoter containing 9 reverse…

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn-GRAB_ACh3.0

    Plasmid

    #121922

    Purpose

    express the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0

    Depositor

    Yulong Li

    Article

    Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s

    Has Service

    AAV9

    Insert

    GPCR activation based ACh sensor GRAB-ACh3.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21b(+)-Is-PETase-W159H-S238F

    Plasmid

    #112203

    Purpose

    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W159H and S238F mutations, codon optimized for expression in E. coli K12

    Depositor

    Gregg Beckham, Christopher Johnson

    Article

    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

    Insert

    PETase gene from Ideonella sakaiensis 201-F6 with W159H and S238F mutations, codon optimized for expression in E. coli K12
    

    Tags

    6XHIS

    Expression

    Bacterial

    Mutation

    W159H, S238F

    Promoter

    N/A

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. lenti-SpCas9 neo

    Plasmid

    #104996

    Purpose

    This lentiviral construct delivers hSpCas9 and G418 resistance.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    KpnI-XhoI-BsrGI-NheI
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti CMVtight Puro DEST (w768-1)

    Plasmid

    #26430

    Depositor

    Eric Campeau

    Type

    Empty backbone

    Use

    Lentiviral; Tet-on advanced destination vector

    Expression

    Mammalian

    Available Since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28-MBP-super TEV protease

    Plasmid

    #171782

    Purpose

    Expresses Maltose-binding protein-super TEV protease in bacterial cytoplasm. This protease version performs well in the absence of added reducing agent.

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    MBP-sTEV
    

    Tags

    Arg6 tag and Internal His6 tag

    Expression

    Bacterial

    Mutation

    No internal TEV cleavage site between the MBP and…

    Promoter

    T7

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLentiCMV Puro DEST ERKKTRClover

    Plasmid

    #59150

    Purpose

    Lentiviral vector to express ERK KTR mClover under CMV promoter (With Puromycin Resistance)

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    ERK Kinase Translocation Reporter
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSIRV-AP-1-mCherry

    Plasmid

    #118095

    Purpose

    Fluorescent reporter for AP-1 activation

    Depositor

    Peter Steinberger

    Article

    Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

    Insert

    mCherry
    

    Use

    Retroviral

    Promoter

    minimal promoter with AP-1 responsive element

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pY004 (pcDNA3.1-hFnCpf1)

    Plasmid

    #69976

    Purpose

    Expresses humanized FnCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hFnCpf1
    

    Use

    CRISPR

    Tags

    NLS & 3xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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