8,806 results

1. pSpCas9 (PX165)

   Plasmid

   #48137

   Purpose

   Human codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLIK-Hygro

   Plasmid

   #25737

   Purpose

   3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Hygromycin resistance gene coexpression.

   Depositor

   Iain Fraser

   Article

   Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Aug. 13, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJL 89

   Plasmid

   #98558

   Purpose

   Useful for making infectious clones of RNA virus cDNAs; Has 35S promoter and HDV Ribozyme sequences in T-DNA region of Agrobacterium vector.

   Depositor

   John Lindbo

   Article

   Lindbo et al Plant Physiol. 2007 Dec;145(4):1232-40.

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   Enh 35S

   Available Since

   Aug. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-MetGFP

   Plasmid

   #37560

   Depositor

   David Rimm

   Insert

   MET (MET Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. anxA2-GFP

   Plasmid

   #107196

   Purpose

   Expresses human annexin A2-GFP fusion protein for live cell imaging

   Depositor

   Volker Gerke, Ursula Rescher

   Article

   Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

   Insert

   annexin A2 (ANXA2 Human)
   

   Tags

   Green Fluorescent Protein

   Expression

   Mammalian

   Mutation

   Ala residue 65 in human sequence replaced by Glu …

   Promoter

   CMV

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCardinal-Lifeact-7

   Plasmid

   #54663

   Purpose

   Localization: Actin, Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Type

   Empty backbone

   Tags

   mCardinal-Lifeact

   Expression

   Mammalian

   Available Since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pcDNA-hHSF1 WT

   Plasmid

   #71724

   Purpose

   plasmid expressing myc-his tag hHSF1 WT

   Depositor

   Lea Sistonen

   Article

   Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

   Insert

   HSF1 (HSF1 Human)
   

   Tags

   MYC 6xHIS

   Expression

   Mammalian

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. reporter-gT1

   Plasmid

   #47320

   Purpose

   TF reporter for gRNA-AAVS1_T1 (RNA-guided dTomato expression)

   Depositor

   George Church

   Article

   Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

   Insert

   minCMV-dTomato_pA
   

   Use

   CRISPR

   Available Since

   Aug. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-Caveolin-C-10

   Plasmid

   #55008

   Purpose

   Localization: Membrane, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Article

   Hanson et al Traffic. 2013 Jun;14(6):663-77. doi: 10.

   Insert

   Caveolin (CAV1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. HDAC8 Flag

    Plasmid

    #13825

    Depositor

    Eric Verdin

    Article

    Waltregny et al FASEB J. 2005 Jun . 19(8):966-8.

    Insert

    HDAC8 (HDAC8 Human)
    

    Tags

    flag

    Expression

    Mammalian

    Available Since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR/D_creERt2

    Plasmid

    #27321

    Depositor

    Leonard Zon

    Article

    Mosimann et al Development. 2011 Jan . 138(1):169-77.

    Insert

    creERt2
    

    Use

    Multisite gateway middle entry vector

    Available Since

    Jan. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETcon(-)

    Plasmid

    #41522

    Depositor

    Andrew Scharenberg

    Type

    Empty backbone

    Tags

    3x(G4S) Linker, G3S Linker, HA, Myc Tag, and Stuf…

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. mRuby2-Lifeact-7

    Plasmid

    #54674

    Purpose

    Localization: Actin, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Type

    Empty backbone

    Tags

    LifeAct-mRuby2

    Expression

    Mammalian

    Available Since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pFA6a-6xGLY-V5-hphMX4

    Plasmid

    #20779

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Expression

    Yeast

    Available Since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR5'_ubi:loxP-EGFP-loxP

    Plasmid

    #27322

    Depositor

    Leonard Zon

    Article

    Mosimann et al Development. 2011 Jan . 138(1):169-77.

    Insert

    ubiquitin:loxP-EGFP-loxP
    

    Use

    Multisite gateway 5' entry vector

    Available Since

    April 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. 3xFLAG-dCas9/pCMV-7.1

    Plasmid

    #47948

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al Biochem Biophys Res Commun. 2013 Sep 13;

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A (catalyticall…

    Promoter

    CMV

    Available Since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. gRNA_AAVS1-T1

    Plasmid

    #41817

    Purpose

    Expresses a guide RNA (gRNA) to target human AAVS1 (T1 target sequence) for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    gRNA_AAVS1-T1
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAM2991

    Plasmid

    #40248

    Depositor

    Susan Golden

    Article

    Ivleva et al EMBO J. 2005 Mar 23;24(6):1202-10. Epub

    Insert

    One Step Cloneing Vector for Overexpression
    

    Use

    Cyanobacteria cloning vector

    Promoter

    n/a

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJMK001

    Plasmid

    #33780

    Depositor

    Adam Cohen

    Article

    Kralj et al Science. 2011 Jul 15;333(6040):345-8.

    Insert

    Proteorhodopsin Optical Proton Sensor
    

    Expression

    Bacterial

    Mutation

    D97N

    Promoter

    AraBAD

    Available Since

    Dec. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pICH47761

    Plasmid

    #48003

    Depositor

    Sylvestre Marillonnet

    Article

    Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    Dec. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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