8,884 results

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tetO-TBX5

Plasmid

#46032

Depositor

John Gearhart

Article

Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

Insert

TBX5 (TBX5 Human)

Use

Lentiviral

Tags

His and V5

Expression

Mammalian

Promoter

tetO

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only

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cfSGFP2-N

Plasmid

#37533

Depositor

Ikuo Wada

Article

Suzuki et al PLoS One. 2012;7(5):e37551. Epub 2012 Ma

Insert

cysteine-free SGFP2-N

Expression

Mammalian

Mutation

C48S and C70M mutations relative to SGFP2

Promoter

CMV

Available Since

Aug. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pXL002-ishRNA-beta-catenin-1

Plasmid

#36297

Depositor

Sean Palecek

Article

Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

Insert

shRNA of beta-catenin-1 (CTNNB1 Human)

Use

Lentiviral and RNAi

Tags

IRES-RFP-Puro

Expression

Mammalian

Promoter

TetO-H1

Available Since

Aug. 7, 2012

Availability

Academic Institutions and Nonprofits only
pET21b-Casp1-His

Plasmid

#11809

Depositor

Guy Salvesen

Insert

Caspase-1 (CASP1 Human)

Tags

His

Expression

Bacterial

Available Since

May 12, 2006

Availability

Academic Institutions and Nonprofits only

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Cortactin-pmCherryC1

Plasmid

#27676

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Cortactin (Cttn Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

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pET15b CnA CnB

Plasmid

#11787

Depositor

Anjana Rao

Article

Mondragon et al Biochemistry. 1997 Apr 22. 36(16):4934-4

Insert

CnA CnB (PPP3R1, PPP3CA Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

May 4, 2006

Availability

Academic Institutions and Nonprofits only
pLKO.1 puro CXCR4 siRNA-2

Plasmid

#12272

Depositor

Bob Weinberg

Article

Orimo et al Cell. 2005 May 6. 121(3):335-48.

Insert

CXCR4 siRNA (CXCR4 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only

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AAVS1-Blasticidin-CAG-Flpe-ERT2

Plasmid

#68461

Purpose

AAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and blasticidin selection gene

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Insert

mammalian-codon-optimized Flpe recombinase fused with ERT2

Use

Synthetic Biology

Tags

ERT2

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pCX-EGFP beta5 integrin receptor

Plasmid

#14996

Depositor

Raymond Birge

Article

Albert et al Nat Cell Biol. 2000 Dec . 2(12):899-905.

Insert

beta5 integrin receptor (ITGB5 Human)

Tags

IRES EGFP

Expression

Mammalian

Available Since

Aug. 22, 2007

Availability

Academic Institutions and Nonprofits only

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pGEX6p1-hPdcd4

Plasmid

#20693

Depositor

Haiwei Song

Article

Loh et al EMBO J. 2009 Feb 4. 28(3):274-85.

Insert

Programmed Cell Death Protein 4 (PDCD4 Human)

Tags

GST

Expression

Bacterial

Mutation

I36V- does not alter function

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only
pCB163

Plasmid

#37388

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

Insert

Dynactin 2 (DCTN2 Human)

Use

Retroviral

Tags

GFP, S-tag, and TEV

Expression

Mammalian

Mutation

A7P

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-eNpHR 3.0-EYFP

Plasmid

#26971

Purpose

AAV expression of eNpHR 3.0 fused to EYFP driven by CaMKIIa promoter for optogenetic inhibition

Depositor

Karl Deisseroth

Has Service

AAV1 and AAV9

Insert

eNpHR 3.0

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

Trafficking Signal (TS) ER Export Signal

Promoter

CaMKIIa

Available Since

Feb. 4, 2011

Availability

Academic Institutions and Nonprofits only

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3XFlagNICD4

Plasmid

#20186

Depositor

Raphael Kopan

Article

Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

Insert

Notch 4 Intracellular Domain (Notch4 Mouse)

Tags

3X Flag

Expression

Mammalian

Mutation

Contains murine Notch 4 Intracellular Domain (Val…

Promoter

CMV

Available Since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only
pDONR223-NTRK1

Plasmid

#23891

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NTRK1 (NTRK1 Human)

Use

Gateway donor vector

Mutation

V263L relative to NM_001012331

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pEGFP Cep164 (Nigg CW324)

Plasmid

#41149

Depositor

Erich Nigg

Article

Graser et al J Cell Biol. 2007 Oct 22;179(2):321-30.

Insert

Cep164 (CEP164 Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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CLC-pmCherryC1

Plasmid

#27680

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Clathrin Light Chain (Clta Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

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mEos3.2-Tubulin-C-18

Plasmid

#57484

Purpose

Localization: Microtubules, Excitation: 507 / 572, Emission: 516 / 580

Depositor

Michael Davidson

Insert

Tubulin (TUBA1B Human)

Tags

mEos3.2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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Flag-SENP1m

Plasmid

#17358

Depositor

Edward Yeh

Article

Cheng et al Cell. 2007 Nov 2. 131(3):584-95.

Insert

SENP1 (SENP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

R630L, K631M

Available Since

March 19, 2008

Availability

Academic Institutions and Nonprofits only

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DRH296: FCK-Optopatch2

Plasmid

#51694

Purpose

Coexpression plasmid of QuasAr2 and CheRiff for all-optical electrophysiology

Depositor

Adam Cohen

Article

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

Optopatch2

Use

Lentiviral

Expression

Mammalian

Promoter

CaMKIIa

Available Since

June 23, 2014

Availability

Academic Institutions and Nonprofits only

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pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B

Plasmid

#64218

Purpose

Expression vector for sgRNA and for Expression of Cas9 linked to BFP via T2A linked to Ad4 E1B via P2A

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA cassette

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-BFP-P2A-E1B

Expression

Mammalian

Promoter

CBh and U6

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM33_Nsp5_Mpro

Plasmid

#156475

Purpose

Bacterial expression of Sars-CoV2 Nsp5_Mpro protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp5_Mpro (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
VCP(A232E)-EGFP

Plasmid

#23973

Depositor

Nico Dantuma

Article

Tresse et al Autophagy. 2010 Feb 22. 6(2):.

Insert

Valosin containing protein (VCP Human)

Tags

6xHis, EGFP, and HA

Expression

Mammalian

Mutation

A232E

Available Since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
CAG:: mCherry-2a-hM4Dnrxn

Plasmid

#52523

Purpose

Co-expresses hM4Dnrxn and a red fluorescent label

Depositor

Scott Sternson

Article

Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

Insert

mCherry-2a-hM4Dnrxn

Tags

2xHA

Expression

Mammalian

Promoter

CAg

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
c-myc promoter (TBE 1/2 - wt)

Plasmid

#16595

Depositor

Bert Vogelstein

Article

He et al Science. 1998 Sep 4. 281(5382):1509-12.

Insert

c-myc promoter (TBE 1/2 wt) (MYC Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

March 21, 2008

Availability

Academic Institutions and Nonprofits only
pINDUCER-21-SPI1

Plasmid

#97039

Purpose

Expresses SPI1 in mammalian cells

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Spi-1 Proto-Oncogene (SPI1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only
CaV1.3e[8a,11,31b,Δ32,42a]

Plasmid

#49333

Purpose

Repaired Addgene #26576 to agree with the rat ref sequence. Repairs are @: nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].

Depositor

Diane Lipscombe

Article

Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

Insert

CACNA1D (Cacna1d Rat)

Expression

Mammalian

Mutation

Contains exons 8a, 11, 31b and 42a.Deletion of ex…

Promoter

CMV

Available Since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pQLinkN

Plasmid

#13670

Depositor

Konrad Buessow

Article

Scheich et al Nucleic Acids Res. 2007;35(6):e43. doi:

Insert

multi cloning site

Use

Co-expression

Expression

Bacterial

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
TRF2-IRESS-eGFP

Plasmid

#19798

Depositor

Mark Mattson

Article

van Steensel et al Cell. 1998 Feb 6. 92(3):401-13.

Insert

TRF2 (TERF2 Human)

Expression

Mammalian

Mutation

When compared to GenBank reference sequence NP_00…

Promoter

CMV-F

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
FUW-tetO-hKLF4

Plasmid

#20725

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Cell Stem Cell. 2008 Sep 11. 3(3):346-53

Insert

KLF4 (KLF4 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1mychisA XDH

Plasmid

#10958

Depositor

Toren Finkel

Article

Ohtsubo et al Circ Res. 2004 Nov 26. 95(11):1118-24.

Insert

xanthine dehydrogenase (XDH Human)

Tags

his and myc

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pLOVE-Sox2

Plasmid

#15953

Depositor

Miguel Ramalho-Santos

Article

Blelloch et al Cell Stem Cell. 2007 Sep 13;1(3):245-7.

Insert

SRY (sex determining region Y)-box 2 (Sox2 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

The recombination reaction required to clone &quo…

Available Since

Nov. 7, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pOZ-N-FH RAP80

Plasmid

#27501

Depositor

Roger Greenberg

Article

Patterson-Fortin et al J Biol Chem. 2010 Oct 1. 285(40):30971-8

Insert

RAP80 (UIMC1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

March 1, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Myc CDC4 WT*

Plasmid

#16652

Depositor

Bert Vogelstein

Article

Rajagopalan et al Nature. 2004 Mar 4. 428(6978):77-81.

Insert

CDC4 (FBXW7 Human)

Tags

myc

Expression

Mammalian

Mutation

*Note that this insert is only WT relative to the…

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAC-BA-lacZ

Plasmid

#13423

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

BA

Use

Cre/Lox

Expression

Bacterial

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
TDP43 NOTAG1

Plasmid

#28206

Depositor

Zuoshang Xu

Article

Yang et al PLoS One. 2010 . 5(12):e15878.

Insert

Tar DNA binding protein (TARDBP Human)

Expression

Mammalian

Mutation

Full length

Available Since

July 1, 2011

Availability

Academic Institutions and Nonprofits only
pCMV-Neo-Bam p53 V143A

Plasmid

#16435

Depositor

Bert Vogelstein

Article

Baker et al Science. 1990 Aug 24. 249(4971):912-5.

Insert

p53 V143A (TP53 Human)

Expression

Mammalian

Mutation

V143A. Mutant cDNA from human colorectal tumor CX…

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pRK7-FLAG-TSC2

Plasmid

#8996

Depositor

John Blenis

Article

Tee et al Proc Natl Acad Sci U S A. 2002 Oct 15. 9

Insert

TSC2 (TSC2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEM-HE-h_bPAC_cmyc

Plasmid

#28134

Purpose

optogenetic tool for light modulation of cellular cAMP

Depositor

Peter Hegemann

Article

Stierl et al J Biol Chem. 2011 Jan 14. 286(2):1181-8.

Insert

humanized photoactivated adenylyl cyclase

Use

In vitro transcription

Tags

Myc

Available Since

June 23, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+8CeGFP

Plasmid

#34952

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Type

Empty backbone

Use

Sea urchin, zebrafish, xenopus

Tags

eGFP

Expression

Mammalian

Promoter

SP6

Available Since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E4orf6

Plasmid

#64222

Purpose

Expression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry linked to the Ad4 E4orf6 gene via P2A

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA cassette

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-mCherry-P2A-E4orf6

Expression

Mammalian

Promoter

CBh and U6

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge

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8,884 results