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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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177,170 results

Showing: 9101 - 9150 of 177170 results

  1. mito-meGFP

    Plasmid
    #172481
    Purpose
    meGFP targeted to the mitochondrial matrix with a human COX8 presequence
    Depositor
    Thomas Schwarz
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Mito (COX8A Human)
    Tags
    meGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    172481-plasmid-map-sequence-id-345344

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  2. Gi2-CASE

    Plasmid
    #168121
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi2. Composed of the subunits G alpha i2 (GNAI2) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).
    Depositor
    Gunnar Schulte
    Publication
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GNAI2, GNB1, GNG2 (GNAI2, GNB1, GNG2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG2
    Expression
    Mammalian
    Mutation
    NLuc is inserted at C112/E115 within GNAI2
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    168121-plasmid-map-sequence-id-332861

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  19. pSL0284 (pQCascade_entry)

    Plasmid
    #130635
    Purpose
    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array contains two BsaI sites for spacer cloning.
    Depositor
    Samuel H. Sternberg
    Publication
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    130635-plasmid-map-sequence-id-255022

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  25. pLenti-EB1-EGFP

    Plasmid
    #118084
    Purpose
    Expresses EB1-EGFP in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Insert
    human EB1 (MAPRE1 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Promoter
    EF1 alpha
    Available since
    Dec. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    118084-plasmid-map-sequence-id-230385

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  40. U6-sgRNA(MS2)_EF1a-MS2-P65-HSF1

    Plasmid
    #92120
    Purpose
    Expression plasmid for both MS2-P65-HSF1 activator helper complex and sgRNA with two MS2 loops at tetraloop and stemloop 2 contains BsaI sites for insertion of spacer sequences.
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MS2-P65-HSF1 (HSF1 Synthetic, Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    92120-plasmid-map-sequence-id-197614

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  42. pJL 89

    Plasmid
    #98558
    Purpose
    Useful for making infectious clones of RNA virus cDNAs; Has 35S promoter and HDV Ribozyme sequences in T-DNA region of Agrobacterium vector.
    Depositor
    John Lindbo
    Publication
    Lindbo et al Plant Physiol. 2007 Dec;145(4):1232-40.
    Type
    Empty backbone
    Expression
    Plant
    Promoter
    Enh 35S
    Available since
    Aug. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    98558-plasmid-map-sequence-id-192654

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  44. pcDNA3-TrxRFP1

    Plasmid
    #98996
    Purpose
    mammalian expression of a biosensor to monitor thioredoxin redox dynamics
    Depositor
    Huiwang Ai
    Publication
    Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human thioredoxin 1, GS-rich linker, fused with a redox-sensitive RFP
    Tags
    His-tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    98996-plasmid-map-sequence-id-190084

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  49. bZiPro

    Plasmid
    #86846
    Purpose
    express NS2B-NS3 protease in E.coli
    Depositor
    Dahai Luo
    Publication
    Zhang et al Science. 2016 Dec 8. pii: aai9309.
    Inserts
    NS3 1-177
    NS2B45-96
    Tags
    a hexahistidine tag and a thrombin cleavage site
    Expression
    Bacterial
    Mutation
    NS2B cofactor region (residues 45–96) and NS3 pro…
    Promoter
    T7
    Available since
    March 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    86846-plasmid-map-sequence-id-172845

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  50. pFA6a-HaloTag-KanMX6

    Plasmid
    #87029
    Purpose
    Tag S. pombe genes in C terminal with HaloTag
    Depositor
    Julien Berro
    Insert
    HaloTag
    Expression
    Bacterial and Yeast
    Available since
    Feb. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    87029-plasmid-map-sequence-id-173001

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Showing: 9101 - 9150 of 177170 results