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Showing: 9501 - 9550 of 157134 results

  3. pFRT-TODestRFP_HuD

    Plasmid
    #65766
    Purpose
    expresses RFP-tagged HuD in mammalian cells
    Depositor
    Thomas Tuschl
    Insert
    ELAVL4 (ELAVL4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    V23M mutation in RFP
    Promoter
    CMV, 2x Tet
    Available since
    Aug. 13, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. pCAGGs-RFP

    Plasmid
    #127347
    Purpose
    Expresses RFP constitutively via the CAGGS promoter in mammalian cells
    Depositor
    Phil Sharp
    Insert
    Red Fluorescent Protein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAGGS Promoter
    Available since
    Dec. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    127347-plasmid-map-sequence-id-246568

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  8. pETM-11-6His-DmBelle_B

    Plasmid
    #145950
    Purpose
    Bacterial Expression of DmBelle
    Depositor
    Elisa Izaurralde
    Insert
    DmBelle (bel Fly)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    145950-plasmid-map-sequence-id-407048

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  9. TAF1L

    Plasmid
    #39014
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TAF1L (TAF1L Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  14. pSPIH6

    Plasmid
    #190676
    Purpose
    E. coli plasmid for single step cloning of SUMO-peptide-intein (SPI) fusion proteins (contains SUMO and His-tagged intein)
    Depositor
    John Vederas
    Article
    Lamer et al BMC Biotechnol. 2023 Apr 5;23(1):11. doi
    Inserts
    Small ubiquitin-like modifier
    Mxe GyrA intein with C-terminal chitin binding domain and His tag
    Use
    Tags
    Chitin binding domain, His tag, and N-terminal Hi…
    Expression
    Bacterial
    Mutation
    Added C-terminal his tag and None
    Promoter
    T7
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    190676-plasmid-map-sequence-id-383624

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  17. pENTR11 WT NFAT1

    Plasmid
    #11791
    Depositor
    Anjana Rao
    Insert
    NFAT1 (Nfatc2 Mouse)
    Use
    Tags
    HA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    11791-plasmid-map-sequence-id-7060

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  29. pOpen-phi29pol

    Plasmid
    #165573
    Purpose
    DNA polymerase responsible for protein-primed viral DNA replication by strand displacement with high processivity and fidelity. Possesses three enzymatic activities: DNA synthesis (polymerase), primer terminal protein (TP) deoxynucleotidylation, and 3' to 5' exonuclease activity.
    Depositor
    FreeGenes Project, Jennifer Molloy, Drew Endy
    Insert
    phi29 DNA Polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165573-plasmid-map-sequence-id-340935

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  33. pCAG-T3-CjCAS9-pA

    Plasmid
    #131543
    Purpose
    Expresses Campylobacter jejuni-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys
    Depositor
    Wataru Fujii
    Article
    Fujii et al Int J Mol Sci. 2017 Oct 31;18(11). pii:
    Insert
    CjCas9
    Use
    CRISPR
    Tags
    FLAG-SV40NLS, SV40NLS, and polyA
    Expression
    Mammalian
    Mutation
    Human codon-optimized
    Promoter
    CAG, T3
    Available since
    Nov. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    131543-plasmid-map-sequence-id-262486

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  34. BK beta1 ECFP

    Plasmid
    #113561
    Purpose
    mouse BK beta1 subunit in C-terminal translational frame with ECFP
    Depositor
    Robert Brenner
    Insert
    Kcnmb1 (Kcnmb1 Mouse)
    Use
    Tags
    ECFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    113561-plasmid-map-sequence-id-218926

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  35. PhOTO-C (pMTB:cyto-Dendra2-2A-H2B-Cerulean)

    Plasmid
    #92402
    Purpose
    Constitutive expression of cytosolic green-to-red photoconvertible Dendra2 and the nuclear blue fluorescent protein Cerulean.
    Depositor
    Periklis Pantazis
    Article
    Kalyviotis et al (2020) Behavioral and Neural Genetics of
    Inserts
    Dendra2
    Cerulean
    Use
    Zebrafish expression
    Tags
    H2B tag and No tag, cytosolic expression
    Expression
    Mutation
    Promoter
    bactin2
    Available since
    July 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    92402-plasmid-map-sequence-id-182712

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  40. pVITRO1-102.1F10-IgM/λ

    Plasmid
    #61880
    Purpose
    Expresses grass pollen allergen Phl p 7 specific human IgM/λ antibody isotype (102.1F10-IgM/λ)
    Depositor
    Andrew Beavil
    Article
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    grass pollen allergen Phl p 7 specific human Mu heavy chain expression cassette
    grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mEF1 Prom and rEF1 Prom
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    61880-plasmid-map-sequence-id-101189

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  49. pET28 E2 LBD-Tev-E1a-His

    Plasmid
    #232117
    Purpose
    For bacterial expression of a fusion protein containing from N to C-terminus: the lipoyl-binding domain of Human E2, a TEV protease site, a peptide from Human E1a, and a His tag.
    Depositor
    Ruth Sommese
    Article
    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi
    Insert
    DBT (DBT Human)
    Use
    Tags
    6xHis, RIGHHSTSDDSSAY (AA331 to 345 (preprocessin…
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232117-plasmid-map-sequence-id-465790

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Showing: 9501 - 9550 of 157134 results