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173,227 results

Showing: 9501 - 9550 of 173227 results

  1. pBJG1 T7-8xHis-OGT K842M

    Plasmid
    #154272
    Purpose
    Bacterial expression of full length human OGT with K842M mutation (catalytically inactive). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.
    Depositor
    Suzanne Walker
    Article
    Lazarus et al Nature. 2011 Jan 27;469(7331):564-7. doi
    Insert
    O-GlcNAc Transferase (OGT Human)
    Tags
    8x His, HRV3C protease site, and T7 leader
    Expression
    Bacterial
    Mutation
    Lysine 842 changed to Methionine- bacterial codon…
    Available Since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    154272-plasmid-map-sequence-id-304833

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  3. Talin-TS

    Plasmid
    #83376
    Purpose
    FRET probe for measuring tension on Talin molecule
    Depositor
    Martin Schwartz
    Article
    Kumar et al J Cell Biol. 2016 May 9;213(3):371-83. d
    Insert
    Talin1 (Tln1 Mouse)
    Use
    Retroviral
    Tags
    EGFP-F40-tagRFP (tension sensor module)
    Expression
    Mammalian
    Mutation
    Tension sensor module is inserted between V447 an…
    Promoter
    CMV
    Available Since
    Oct. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    83376-plasmid-map-sequence-id-158760

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  13. pCfB2337

    Plasmid
    #67555
    Purpose
    EasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae XII-5 chromosomal location, USER site for cloning, amp resistance
    Depositor
    Irina Borodina
    Article
    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.
    Type
    Empty backbone
    Use
    Cre/Lox; Integrative
    Expression
    Yeast
    Available Since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    67555-plasmid-map-sequence-id-116237

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  17. lentiCRISPR.v2.TetON_Cas13d_U6_DR

    Plasmid
    #196727
    Purpose
    Sortable and selectable, Tet-inducible RfxCas13d with guide cassette (one vector system). Inducible knock-down.
    Depositor
    Bernhard Schmierer
    Insert
    RfxCas13d-T2A-miRFP670nano
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Promoter
    TRE
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196727-plasmid-map-sequence-id-389689

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  20. AICSDP-60: SOX2-mEGFP

    Plasmid
    #124606
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human SOX2
    Depositor
    Allen Institute for Cell Science
    Insert
    SOX2 Homology Arms with linker-mEGFP (SOX2 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    May 13, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    124606-plasmid-map-sequence-id-243801

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  21. Ultra-Exon1Q145-Myc-A

    Plasmid
    #110487
    Purpose
    Lentiviral vector to express mutant N terminal HTT gene with short 3'UTR in mammalian cells (encodes Myc-tagged mutant huntingtin exon1 fragment with 145 repeats of Q)
    Depositor
    Baoji Xu
    Article
    Xu et al PLoS One. 2017 May 11;12(5):e0177610. do
    Insert
    Homo sapiens huntingtin (HTT Human)
    Use
    Lentiviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Partial long 3'UTR wild type huntingtin exon…
    Promoter
    UbC
    Available Since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    110487-plasmid-map-sequence-id-212608

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  22. CMV-GFP-Kir2.1

    Plasmid
    #107184
    Purpose
    mammalian expression of Kir2.1
    Depositor
    Bikash Pattnaik
    Insert
    KCNJ2 (KCNJ2 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    107184-plasmid-map-sequence-id-203927

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  27. pHR_Gal4UAS_4D5-High-CAR-mCherry_PGK_4D5-Low-SynNotch_Gal4VP64_RHL133

    Plasmid
    #164822
    Purpose
    Lentiviral vector for Gal4 inducible AntiHER2 4D5-High CAR-mCherry and constitutive expression of antiHER2 4D5-Low Gal4VP64 Synthetic Notch
    Depositor
    Wendell Lim
    Article
    Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171
    Inserts
    AntiHER2 4D5-High Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
    AntiHER2 4D5-Low Gal4VP64 synthetic Notch receptor
    Use
    Lentiviral
    Tags
    mCherry
    Promoter
    miniCMV and pGK
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    164822-plasmid-map-sequence-id-330530

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  29. mCherry-Occludin-N-10

    Plasmid
    #55112
    Purpose
    Localization: Tight Junctions, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Occludin (OCLN Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55112-plasmid-map-sequence-id-257392

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  31. pcDNA3-TLR10-YFP

    Plasmid
    #13643
    Depositor
    Doug Golenbock
    Insert
    Toll-Like Receptor 10 (TLR10 Human)
    Tags
    YFP
    Expression
    Mammalian
    Available Since
    June 12, 2007
    Availability
    Academic Institutions and Nonprofits only
    13643-plasmid-map-sequence-id-7721

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  32. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP

    Plasmid
    #118156
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-EGFP
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    EF1a core and U6
    Available Since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    118156-plasmid-map-sequence-id-239644

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Showing: 9501 - 9550 of 173227 results