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173,772 results

Showing: 9651 - 9700 of 173772 results

  1. VP12

    Plasmid
    #72247
    Purpose
    Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A and Q926A
    Promoter
    CMV
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72247-plasmid-map-sequence-id-485302

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  12. pBluescript-PB

    Plasmid
    #133885
    Purpose
    piggyBac ITR's in high copy number pBluescript backbone plasmid
    Depositor
    Mario Capecchi
    Type
    Empty backbone
    Use
    Piggybac
    Available Since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    133885-plasmid-map-sequence-id-274419

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  13. pFFLuc-IVT

    Plasmid
    #205147
    Purpose
    IVT template of FireFly luciferase stop codon between the ORF and MCS and polyA
    Depositor
    Igor Ulitsky
    Insert
    FireFly luciferase
    Use
    Luciferase; In-vitro transcription
    Available Since
    Aug. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    205147-plasmid-map-sequence-id-495367

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  14. pRECBRR1-Kva1

    Plasmid
    #240703
    Purpose
    inverted BRR1 origin of replication plasmid containing Kva1 recombitron with a donor in the ncRNA to target phzM locus in Pseudomonas aeruginosa PAO1 expressed by Pm promoter
    Depositor
    Seth Shipman
    Article
    Gonzalez-Delgado et al Nat Biotechnol. 2026 Apr 23. doi: 10.103
    Insert
    Kva1 RT,Kva1 ncRNA, PaRecT and PaSSB
    Expression
    Bacterial
    Mutation
    Inverted BRR1 origin of replication
    Available Since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240703-plasmid-map-sequence-id-483567

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  16. pHelper_TS_V2_ampR

    Plasmid
    #214467
    Purpose
    Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Includes temperature sensitive origin for plasmid curing.
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Insert
    CspRecT / Bxb-1
    Use
    Synthetic Biology
    Promoter
    XylS / AraC
    Available Since
    April 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    214467-plasmid-map-sequence-id-429338

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  17. pOpen-MMLV_RT (lack H)

    Plasmid
    #165556
    Purpose
    Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity. Comparable to SuperScriptII from Thermo Fisher.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain)
    Use
    Synthetic Biology
    Available Since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165556-plasmid-map-sequence-id-339910

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  18. TRUPATH Triple Ga15

    Plasmid
    #196060
    Purpose
    Encodes a G alpha subunit (GNA15) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlpha15-RLuc8 (GNA15 Human)
    GGamma13-GFP2 (GNG13 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196060-plasmid-map-sequence-id-385870

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  22. AAV-CamK2-tTA

    Plasmid
    #210731
    Purpose
    AAV vector expressing tetracycline-controlled transactivator (tTA) under CamK2 promoter (for expressing tTA in glutamatergic neurons)
    Depositor
    Wei Xu
    Article
    Thompson et al Cell Rep Methods. 2023 Dec 18;3(12):1006
    Insert
    tetracycline-controlled transactivator (tTA)
    Use
    AAV
    Promoter
    CamK2
    Available Since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    210731-plasmid-map-sequence-id-418042

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  27. pET-16b-10His-TEV-MCES_VACCA_D1_D12

    Plasmid
    #203671
    Purpose
    The D1 and D12 subunits of the vaccinia capping enzyme were cloned in tandem into the vector pET-16b (Novagen). An N-terminal His10 tag was added to the D1 subunit and the original factor Xa cleavage
    Depositor
    Stephen Cusack
    Article
    Kyrieleis et al Structure. 2014 Mar 4;22(3):452-65. doi:
    Insert
    MCES_VACCA_D1_D12
    Tags
    10xHis
    Expression
    Bacterial
    Available Since
    Aug. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    203671-plasmid-map-sequence-id-403167

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  34. pLV-SFFV-MCS-WPRE-UbC-mCherry

    Plasmid
    #211799
    Purpose
    Lentiviral vector plasmid containing a multiple cloning site (MCS) under the spleen focus-forming virus (SFFV) promoter and expressing mCherry under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Inserts
    multiple cloning site
    mCherry
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    SFFV and UbC
    Available Since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211799-plasmid-map-sequence-id-418073

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  35. PCDH-METTL16-3*Flag

    Plasmid
    #196207
    Purpose
    overexpress METTL16
    Depositor
    Jianjun Chen
    Insert
    WT METTL16 (METTL16 Human)
    Use
    Lentiviral
    Mutation
    NO
    Available Since
    Feb. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196207-plasmid-map-sequence-id-383845

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  36. pNJP

    Plasmid
    #115494
    Purpose
    Expression vector for NLS-iRFP-NLS, JNK KTR-mCherry, and mKO-MK2 in mammalian cells
    Depositor
    Kazuhiro Aoki
    Article
    Miura et al Cell Rep. 2018 Sep 4;24(10):2658-2668. d
    Insert
    NLS-iRFP-NLS-P2A-JNK KTR-mCherry-P2A-mKO-MK2 (MAPK14, MAPK8 Synthetic, Human, Mouse)
    Tags
    iRFP, mCherry, and mKO
    Expression
    Mammalian
    Mutation
    mCherry S227F, mCherry G229R, mKO V211G
    Promoter
    CAG
    Available Since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    115494-plasmid-map-sequence-id-231521

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  39. PM-B

    Plasmid
    #209876
    Purpose
    Dimerization dependent fluorescent protein B anchored to cytosolic face of the plasma membrane with the N-terminal targeting sequence of human GAP43
    Depositor
    Sarah Cohen
    Article
    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25
    Insert
    ddGFP B
    Tags
    Targeting domain of Gap43 MLCCMRRTKQVEKNDDQKI
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    209876-plasmid-map-sequence-id-415466

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  42. Mito-GA

    Plasmid
    #209865
    Purpose
    Dimerization dependent fluorescent protein GA anchored to cytosolic face of the mitochondrial membrane with C-terminal targeting sequence of human MAVS
    Depositor
    Sarah Cohen
    Article
    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25
    Insert
    ddGFP A
    Tags
    Targeting domain of MAVS RPSPGALWLQVAVTGVLVVTLLVV…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    209865-plasmid-map-sequence-id-415469

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  44. TFORF0863

    Plasmid
    #143732
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    GTF2I (GTF2I Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Feb. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    143732-plasmid-map-sequence-id-415857

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  48. R777-E352 Hs.TP53-nostop

    Plasmid
    #70636
    Purpose
    Gateway ORF clone of human TP53 [NM_000546.5] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    TP53 (TP53 Human)
    Use
    Gateway Cloning
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70636-plasmid-map-sequence-id-126085

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  49. pTIPI_mIGg2a_Anti-HA [12CA5]

    Plasmid
    #210656
    Purpose
    Mammalian expression of Anti-HA [12CA5] mouse IgG2a heavy chain; to be used with pTIPI_mK_Anti-HA [12CA5] light chain (Plasmid 210657) to make the antibody.
    Depositor
    Melina Fan, Addgene Research Program
    Insert
    Anti-HA [12CA5] mouse IgG2a heavy chain
    Use
    Affinity Reagent/ Antibody
    Tags
    Mouse IgG2a heavy chain
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 18, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    210656-plasmid-map-sequence-id-416221

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  50. pTIPI_mK_Anti-HA [12CA5]

    Plasmid
    #210657
    Purpose
    Mammalian expression of Anti-HA [12CA5] mouse kappa light chain; to be used with pTIPI_mIGg2a_Anti-HA [12CA5] heavy chain (Plasmid 210656) to make the antibody.
    Depositor
    Melina Fan, Addgene Research Program
    Insert
    Anti-HA [12CA5] kappa light chain
    Use
    Affinity Reagent/ Antibody
    Tags
    Mouse kappa light chain
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 18, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    210657-plasmid-map-sequence-id-416222

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Showing: 9651 - 9700 of 173772 results