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169,371 results

Showing: 9951 - 10000 of 169371 results

  5. pCX-EGFP c-Abl

    Plasmid
    #15007
    Depositor
    Raymond Birge
    Insert
    c-Abl (Abl1 Mouse)
    Tags
    IRES EGFP
    Expression
    Mammalian
    Available Since
    Aug. 22, 2007
    Availability
    Academic Institutions and Nonprofits only

  8. pYLCRISPR/Cas9P35S-N

    Plasmid
    #66191
    Purpose
    expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), Neo selection
    Depositor
    Yao-Guang Liu
    Article
    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(
    Insert
    Cas9
    Expression
    Plant
    Mutation
    plant-codon optimized with higher GC contents (62ā€¦
    Promoter
    cauliflower mosaic virus 35S promoter (P35S)
    Available Since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    66191-plasmid-map-sequence-id-404047

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  11. TFORF0756

    Plasmid
    #141685
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    JUND (JUND Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141685-plasmid-map-sequence-id-392755

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  15. pKM427

    Plasmid
    #210788
    Purpose
    L5 integrating vector for delivery of defective Hyg gene (ZeoR) for oligonucleotide-mediated SNP transfer
    Depositor
    Kenan Murphy
    Article
    Murphy et al Methods Mol Biol. 2021;2314:301-321. doi
    Insert
    Defective hygromycin resistant gene
    Expression
    Bacterial
    Mutation
    Changed hygromycin resistant gene W190 codon (TGGā€¦
    Available Since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    210788-plasmid-map-sequence-id-418045

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  16. pSico p53

    Plasmid
    #12089
    Purpose
    Cre-regulated lentiviral shRNA vector. Cre addition causes EGFP to be recombined out of the construct, activating p53 shRNA expression.
    Depositor
    Tyler Jacks
    Article
    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1
    Insert
    p53 shRNA (Trp53 Mouse)
    Use
    Cre/Lox, Lentiviral, and RNAi
    Expression
    Mammalian
    Mutation
    EGFP is expressed from this plasmid as a marker, ā€¦
    Available Since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12089-plasmid-map-sequence-id-222566

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  18. mTurquoise-Clathrin-15

    Plasmid
    #55544
    Purpose
    Localization: Clathrin Vesicles, Excitation: 434, Emission: 474
    Depositor
    Michael Davidson
    Insert
    Clathrin (CLTB Human)
    Tags
    mTurquoise
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 7, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    55544-plasmid-map-sequence-id-263557

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  19. CRISPRoff-mScarletI

    Plasmid
    #217783
    Purpose
    Expresses CRISPRoff-mScarletI (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-mScarletI-KRAB) downstream of the CAG promoter for gene epigenetic silencing
    Depositor
    Adriano Aguzzi
    Article
    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038
    Insert
    CRISPRoff-mScarletI (DNMT3A-DNMT3L-dCas9-mScarletI-KRAB)
    Tags
    2xNLS, DNMT3A-DNMT3L, HA, KRAB, and mScarletI
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    April 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    217783-plasmid-map-sequence-id-468886

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  20. FLX1.8NFATGFPpd2HS4

    Plasmid
    #169096
    Purpose
    Lentiviral vector encoding a short-lived GFP under the transcriptional control of a mutlimerized NFAT gene enhancer to measure T cell activation
    Depositor
    Geoffrey Owens
    Inserts
    NFAT enhancer (from IL-4 promoter)
    Destabilized green fluorescent protein
    Use
    Lentiviral
    Promoter
    NFAT enhancer + synthetic TATA box
    Available Since
    July 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    169096-plasmid-map-sequence-id-338033

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  22. Q-IL6-9

    Plasmid
    #166552
    Purpose
    scFv of a human scaffold targeting Interleukin-6. Antigen coverage aa 28-212 of 212, full-length excl. signal peptide
    Depositor
    Susanne GrƤslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, Sortag, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166552-plasmid-map-sequence-id-328267

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  25. Hs.KRAS4b G12D

    Plasmid
    #83131
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12D mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Mutation
    G12D
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83131-plasmid-map-sequence-id-158094

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  31. pcDNA-HA FoxP3

    Plasmid
    #229615
    Purpose
    Mammalian expression of full length mouse FoxP3
    Depositor
    Sun Hur
    Insert
    full length FoxP3 (Foxp3 Mouse)
    Expression
    Mammalian
    Mutation
    none
    Available Since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    229615-plasmid-map-sequence-id-457337

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  32. pBabe-Kras Wt

    Plasmid
    #75282
    Purpose
    Expresses human Kras wild-type
    Depositor
    Channing Der
    Insert
    KRAS (KRAS Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    None. Wild-Type
    Available Since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    75282-plasmid-map-sequence-id-299863

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  35. NID1-bio-His

    Plasmid
    #52068
    Purpose
    Expresses full-length Nidogen-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    NID1 (NID1 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and raā€¦
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52068-plasmid-map-sequence-id-80293

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  36. pOpen-GBDpol

    Plasmid
    #165501
    Purpose
    Robust and extremely thermostable polymerase with 5x higher fidelity than Taq. Ideal for GC-rich or looped sequences.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Pyrococcus Sp. Heat-Stable DNA Polymerase
    Use
    Synthetic Biology
    Available Since
    July 26, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165501-plasmid-map-sequence-id-338429

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  38. CMV-LUC2CP/ARE

    Plasmid
    #62857
    Purpose
    Splicing reporter control (no intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.
    Depositor
    Gideon Dreyfuss
    Article
    Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d
    Insert
    Luciferase
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    destabilizing sequences (DS) added to the C termiā€¦
    Promoter
    CMV
    Available Since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    62857-plasmid-map-sequence-id-212900

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  41. pCW57.1-FOXM1b

    Plasmid
    #68811
    Purpose
    Doxycycline inducible lentiviral vector for FOXM1b expression in mammalian cells
    Depositor
    Adam Karpf
    Article
    Barger et al Oncotarget. 2015 Jul 16.
    Insert
    FOXM1b (FOXM1 Human)
    Use
    Lentiviral; Doxycycline inducible
    Tags
    HA
    Expression
    Mammalian
    Promoter
    TRE promoter, Tet ON
    Available Since
    Sept. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    68811-plasmid-map-sequence-id-419548

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  49. pDsRed-attP

    Plasmid
    #51019
    Purpose
    Vector for generating dsDNA donors for homology-directed repair to replace genes or other genomic sequence with an attP docking site. Contains the visible marker 3xP3-DsRed. As known as pHD-DsRed-attP
    Depositor
    Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger
    Article
    Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1
    Inserts
    attP
    LoxP-3xP3-DsRed-LoxP
    Use
    High copy, amp resistance
    Promoter
    3xP3 and None
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    51019-plasmid-map-sequence-id-222558

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  50. TFORF3348

    Plasmid
    #144824
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PITX2 (PITX2 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    144824-plasmid-map-sequence-id-391553

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Showing: 9951 - 10000 of 169371 results