165,652 results

1. pCMV-PE7-SB-NLS

   Plasmid

   #228870

   Purpose

   PE7 connected to MLH1-SB-NLS via 2A sequence

   Depositor

   Sangsu Bae

   Article

   Park et al Cell. 2025 Aug 1:S0092-8674(25)00799-8.

   Insert

   PE7-2A-MLH1-SB-NLS
   

   Use

   CRISPR

   Available Since

   Dec. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pENN496_miniCRISPRcharm_dSaCas9

   Plasmid

   #220837

   Purpose

   mini CRISPRcharm expression in mammalian cells

   Depositor

   Jonathan Weissman

   Article

   Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

   Insert

   mini CRISPRcharm
   

   Use

   CRISPR

   Tags

   P2A-TagBFP

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   EFS

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-CAG-iGluSnFR4s-NGR-WPRE

   Plasmid

   #234451

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4s-NGR
   

   Use

   AAV

   Tags

   IgK chain and Myc epi tag-NGR GPI anchor

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pnCas9-PBE

   Plasmid

   #98164

   Purpose

   Expresses CRISPR base editor in protoplasts

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

   Insert

   NLS-APOBEC1-XTEN-nCas9-UGI-NLS
   

   Expression

   Plant

   Promoter

   Ubi

   Available Since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. FLAG-FBXL17

   Plasmid

   #236188

   Purpose

   Expression of N-terminal tagged FLAG-FBXL17 (H. sapiens, codon-optimized) in human cell lines.

   Depositor

   Michele Pagano

   Article

   Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

   Insert

   FLAG-FBXL17 (FBXL17 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. SPHK1 gRNA (BRDN0001147534)

   Plasmid

   #76814

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SPHK1

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   SPHK1 (SPHK1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSMPUW_6.16_ M4_Ef1a-gateway-V5-SV40-Zeo

   Plasmid

   #128452

   Purpose

   lentiviral destination vector with Ef1a promoter, C-terminal V5 tag, SV40 promoter, and Zeo Resistance gene

   Depositor

   Lindy Barrett

   Insert

   Ef1a promoter, C-terminal V5 tag, SV40 promoter, and Zeocin Resistance gene
   

   Use

   Lentiviral

   Available Since

   Jan. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SPHK2 gRNA (BRDN0001146882)

   Plasmid

   #77096

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SPHK2

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   SPHK2 (SPHK2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-PE-Plus

   Plasmid

   #214079

   Purpose

   Mammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and IRES-human MLH1dn

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Insert

   PE-Plus
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Flag-neo-PP1-Alpha

    Plasmid

    #171267

    Purpose

    Mammalian expression of 3x-Flag-tagged human PP1 alpha catalytic subunit

    Depositor

    Steven Balk

    Article

    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

    Insert

    3x-Flag-tagged human PP1 alpha catalytic subunit (PPP1CA Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. eIF2a 1

    Plasmid

    #21807

    Depositor

    David Ron

    Insert

    eIF2a (EIF2S1 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmRFP-LC3

    Plasmid

    #21075

    Purpose

    Mammalian expression of rat LC3 fused to mRFP

    Depositor

    Tamotsu Yoshimori

    Article

    Kimura et al Autophagy. 2007;3(5):452-60.

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    First ATG is not included.

    Available Since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-DsRed_IRES_EGFP

    Plasmid

    #92194

    Purpose

    Lentiviral overexpression vector to make stable bicistronic cell line for control (EGFP) screen

    Depositor

    Huda Zoghbi

    Article

    Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

    Insert

    DsRed-IRES-EGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMK262 (RAD21-mAC Neo)

    Plasmid

    #140538

    Purpose

    RAD21 tagging with mAID-Clover

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    RAD21 (RAD21 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-FLAG-KLF5

    Plasmid

    #40918

    Depositor

    Jin-Tang Dong

    Article

    Zhao et al Biochem J. 2011 Jul 15;437(2):323-33.

    Insert

    Kruppel-like factor 5 (KLF5 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. Str-2-TagBFP-SL2-Pre-SPHERE-SF-iGluSnFR

    Plasmid

    #187099

    Purpose

    Glutamate sensor for localization to membrane contatct

    Depositor

    Kotaro Oka

    Article

    Shindo et al bioRxiv 2022

    Insert

    Pre-SPHERE-SF-iGluSnFR
    

    Tags

    TagBFP-SL2

    Expression

    Worm

    Promoter

    Str-2

    Available Since

    Sept. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. M87

    Plasmid

    #242633

    Purpose

    MYC expression

    Depositor

    Alain Nepveu

    Article

    Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

    Insert

    EGFP-MYC (MYC Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. DAP12-SmBiT BiBiT Ready Vector

    Plasmid

    #238799

    Purpose

    Express DAP12-SmBiT Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    DAP12 (TYROBP Human)
    

    Tags

    SmBiT

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. IL4Ra-HaloTag BiBRET Vector

    Plasmid

    #238676

    Purpose

    Express IL4Ra-HaloTag Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    IL4Ra (IL4R Human)
    

    Tags

    HaloTag (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pLenti-eIF6-3XFlag

    Plasmid

    #242188

    Purpose

    Express eIF6-3xFlag in mammalian cells

    Depositor

    Joshua Mendell

    Article

    Lee et al Mol Cell. 2025 Apr 17;85(8):1543-1560.e1

    Insert

    eIF6 (EIF6 Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Promoter

    CMV

    Available Since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. AiP12320 - pAAV-AiE0475m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2320)

    Plasmid

    #229885

    Purpose

    AiE0475m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific brain cell types

    Depositor

    Jonathan Ting, Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Available Since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGP-AAV-syn-FLEX-Positron2-ST-WPRE

    Plasmid

    #239080

    Purpose

    AAV-mediated expression of positive-going voltage sensor under the Syn promoter, Cre-dependent expression; soma localization targeting sequence

    Depositor

    GENIE Project

    Article

    Eric R. et al Janelia Figshare 2023

    Insert

    Positron2-ST
    

    Use

    AAV and Cre/Lox

    Tags

    soma localization targeting sequence

    Expression

    Mammalian

    Mutation

    R78K N81D D92N W178F

    Promoter

    hSynapsin1

    Available Since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCDH-CB-Cdkn1a

    Plasmid

    #228914

    Purpose

    Expression CDKN1A in mammalian cells

    Depositor

    Sika Zheng

    Article

    Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

    Insert

    Cdkn1a (Cdkn1a Mouse)
    

    Expression

    Mammalian

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCDNA5/FRT_CMV_NES-Kinprola_PKA-mEGFP

    Plasmid

    #233353

    Purpose

    CMV driven expression of the PKA activity recorder Kinprola_PKA in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

    Insert

    NES-Kinprola_PKA-mEGFP
    

    Tags

    NES and mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Str-KDEL_SBP-mCherry-CCR1

    Plasmid

    #222311

    Purpose

    Synchronize the trafficking of CCR1 from the ER.

    Depositor

    Franck Perez

    Article

    Boncompain et al Sci Adv. 2019 Oct 16;5(10):eaax0821. doi

    Insert

    Streptavidin-KDEL and CCR1 fused to SBP-mCherry (CCR1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAGIG-CDKN1A

    Plasmid

    #228912

    Purpose

    Expression CDKN1A in mammalian cells

    Depositor

    Sika Zheng

    Article

    Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

    Insert

    Cdkn1a (Cdkn1a Mouse)
    

    Expression

    Mammalian

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJH4783

    Plasmid

    #200785

    Purpose

    Pnpr-4 snb-1::pHluorin unc-54 3' UTR C.elegans AVA and other neurons expression of snb pHluorin

    Depositor

    Mei Zhen

    Article

    Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

    Insert

    snb-1 (snb-1 Nematode)
    

    Tags

    pHiuorin

    Expression

    Worm

    Promoter

    Pnpr-4

    Available Since

    May 7, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pQE60 R196A wgMDH

    Plasmid

    #204271

    Purpose

    Bacterial expression of R196A wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R196A

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJR032

    Plasmid

    #198773

    Purpose

    Stabilized step function Opsins ChR2 variant

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::hChR2 (C128S, D156A)-EYFP::let-858 3'UTR
    

    Expression

    Worm

    Available Since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJR034

    Plasmid

    #198829

    Purpose

    Step-Waveform Inhibitory Channelrhodopsin

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::SwiCHR-TS-EYFP::let-858 3'UTR
    

    Expression

    Worm

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pJR033

    Plasmid

    #198822

    Purpose

    Chloride-conducting channelrhodopsin

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::UAS-iC1-c2-TS-EYFP::let-858 3'UTR
    

    Expression

    Worm

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pD649-HAsp-TNFSF13B-COMP5AP-AviTag-9xHis

    Plasmid

    #157213

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    TNFSF13B (TNFSF13B Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. RCAS.Sfi

    Plasmid

    #196377

    Purpose

    Empty avian expression vector RCAS with modifications for fixed orientation cloning into SfiI sites

    Depositor

    Peter Vogt

    Article

    Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

    Type

    Empty backbone

    Use

    Retroviral; Avian

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pJH3717

    Plasmid

    #191477

    Purpose

    Pacr-2s-Arch::wCherry unc-54 3' UTR C.elegans A/B MN expression of Arch RFP

    Depositor

    Mei Zhen

    Article

    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

    Insert

    Arch (arch-1 Nematode)
    

    Tags

    wCherry

    Expression

    Worm

    Promoter

    Pacr-2s

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. SPHK1 gRNA (BRDN0001148403)

    Plasmid

    #76815

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SPHK1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SPHK1 (SPHK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. mCherry-TGNP-N-10

    Plasmid

    #55145

    Purpose

    Localization: Golgi Complex, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    TGNP (TGOLN2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCS2-Arl13b-mCardinal

    Plasmid

    #194542

    Purpose

    Expresses Arl13b with mCardinal in mammalian cells and zebrafish embryos

    Depositor

    Heidi Hehnly

    Article

    Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

    Insert

    Arl13b (Arl13b Mouse)
    

    Tags

    mCardinal

    Expression

    Mammalian

    Promoter

    CMV IE94

    Available Since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. CMV-Citron1

    Plasmid

    #134303

    Purpose

    Mammalian expression of direct-response citrate sensor

    Depositor

    Robert Campbell

    Article

    Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

    Insert

    Citron1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pVoPo-GFP

    Plasmid

    #187840

    Purpose

    Constitutive GFP-expression vector for Fusobacterium nucleatum

    Depositor

    Jörg Vogel

    Article

    Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119

    Insert

    sfGFP
    

    Expression

    Bacterial

    Available Since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLenti PGK V5-LUC Neo (w623-2)

    Plasmid

    #21471

    Purpose

    3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Neo

    Depositor

    Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    V5-LUC
    

    Use

    Lentiviral and Luciferase

    Tags

    V5

    Expression

    Mammalian

    Available Since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pLKO.1-sh-mSOX9-5

    Plasmid

    #40646

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    sh-mSOX9-5 (Sox9 Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

42. pEGFPN1-ATG9A

    Plasmid

    #198529

    Purpose

    Expression of ATG9A-GFP fusion protein in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Proc Natl Acad Sci U S A. 2017 Dec 12;11

    Insert

    ATG9A (ATG9A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    silent mutations in codons 4 and 775

    Promoter

    CMV

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. p5155

    Plasmid

    #175391

    Purpose

    Low Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts >15kb) into dual site mouse strains (Bxb-GT/GA)

    Depositor

    Michael Wiles

    Article

    Low et al Sci Rep. 2022 Mar 31;12(1):5424. doi: 10

    Type

    Empty backbone

    Use

    Mouse Targeting; Cloning vector, to be used to de…

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pSH-EFIRES-P-AtAFB2-mCherry

    Plasmid

    #129716

    Purpose

    AAVS1 safe harbor targeting vector expressing AtAFB2-mCherry

    Depositor

    Elina Ikonen

    Article

    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

    Insert

    AFB2 (AFB2 Mustard Weed)
    

    Use

    CRISPR and TALEN; Human safe harbor locus (a avs1…

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    EF1a

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pRMC2-msfGFP

    Plasmid

    #194913

    Purpose

    Tetracycline inducible expression of msfGFP in Staphylococci

    Depositor

    Rikke Meyer

    Article

    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

    Insert

    Shine Dalgarno Sequence followed by monomeric superfolder GFP
    

    Use

    Tetracycline-inducible expression vector for stap…

    Expression

    Bacterial

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pAcCas12nHS_RV6_empty

    Plasmid

    #203811

    Purpose

    Plasmid containing a CMV-driven NLS-AcCas12n-NLS (human-codonoptimized) cassette and a U6-driven sgRNA_V6 cassette (containing BsaI sites for spacer insertion). Carbenicillin resistance.

    Depositor

    Quanjiang Ji

    Article

    Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

    Insert

    AcCas12nHS_RV6
    

    Use

    CRISPR

    Promoter

    CMV

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. TRIPZ-bioGEF-UBnc-PURO

    Plasmid

    #208044

    Purpose

    Enables inducible expression of low-affinity Avitag-UBnc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    UBnc (UBC Human)
    

    Use

    Lentiviral

    Tags

    Low-affinity Avitag

    Mutation

    L73P Mutation near C-terminus suppresses cleavage…

    Promoter

    tetO/UBC

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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48. CVB3 IRES

    Plasmid

    #189968

    Purpose

    Part 2 plasmid to introduce the CVB3 IRES into circRNAs

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    CVB3 IRES
    

    Use

    Cloning

    Promoter

    N/A

    Available Since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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49. pOSIP-KH-RBS2-dCas9

    Plasmid

    #182740

    Purpose

    Integrative plasmid at the HK022 site in E. coli carrying dCas9 under the control of a Ptet promoter

    Depositor

    David Bikard

    Article

    Rousset et al PLoS Genet. 2018 Nov 7;14(11):e1007749.

    Insert

    dCas9
    

    Use

    CRISPR; Integrative vector

    Promoter

    Ptet

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. pWN_U6-B2M-miniGag-Cas9

    Plasmid

    #228958

    Purpose

    miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

    Depositor

    Jennifer Doudna

    Article

    Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

    Insert

    miniGag-Cas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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