We narrowed to 18,147 results for: Eomes

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DsRed2-ER-5

Plasmid

#55836

Purpose

Localization: Endoplasmic Reticulum, Excitation: 558, Emission: 583

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

ER (CALR Human)

Tags

DsRed2

Expression

Mammalian

Mutation

ER Signal Peptide

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pShuttle-CMV-iRFP

Plasmid

#31856

Purpose

a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

Depositor

Vladislav Verkhusha

Article

Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

Insert

iRFP

Use

Adenoviral

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…

Promoter

CMV

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pDEST40-Arl13b11

Plasmid

#40873

Depositor

Tamara Caspary

Article

Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

Insert

Arl13b (Arl13b Mouse)

Tags

His and V5

Expression

Mammalian

Mutation

no stop codon

Promoter

CMV

Available Since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only
HA PKB T308D S473D pcDNA3

Plasmid

#14751

Depositor

Jim Woodgett

Article

Scheid et al Mol Cell Biol. 2002 Sep . 22(17):6247-60

Insert

PKB DD (AKT1 Bovine)

Tags

HA

Expression

Mammalian

Mutation

T308D S473D. Constitutively active mutant.

Available Since

April 12, 2007

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-AQP9

Plasmid

#48808

Purpose

Express human AQP9, which induces filopodia formation

Depositor

Kenneth Jacobson, Vesa Loitto

Article

Loitto et al Exp Cell Res. 2007 Apr 15;313(7):1295-30

Insert

AQP9 (AQP9 Human)

Tags

eGFP

Expression

Mammalian

Available Since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only

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CFP-GAI(1-92)

Plasmid

#37307

Depositor

Takanari Inoue

Article

Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

Insert

GAI (GAI Mustard Weed)

Tags

CFP

Expression

Mammalian

Mutation

contains aa 1-92

Promoter

CMV

Available Since

June 19, 2012

Availability

Academic Institutions and Nonprofits only
pARF1(Q71L)-CFP

Plasmid

#11385

Depositor

Joel Swanson

Article

Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

Insert

ADP Ribosylation Factor 1 (ARF1 Human)

Tags

CFP

Expression

Mammalian

Mutation

Changed Glutamine 71 to Leucine.

Available Since

March 3, 2006

Availability

Academic Institutions and Nonprofits only

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Vmn2r1-5Hprt-loxP TV

Plasmid

#108079

Purpose

Tergeting vector for Vmn2r1 gene knockout mice (∆C1 strain)

Depositor

Peter Mombaerts

Article

Akiyoshi et al Eur J Neurosci. 2018 Apr;47(7):887-900.

Insert

Vmn2r1-Hprt (5')-loxP-neo (Vmn2r1 Mouse)

Use

Mouse Targeting

Available Since

May 17, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST40-Arl13b89

Plasmid

#40874

Depositor

Tamara Caspary

Article

Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

Insert

Arl13b (Arl13b Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-P4:gs:nls:CIBN

Plasmid

#135986

Purpose

Expresses the P4 coiled coil fused to the CIBN blue light-inducible domain in mammalian cells

Depositor

Roman Jerala

Article

Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

Insert

P4 coiled-coil fused to the CIBN domain

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCMVHA E2F2

Plasmid

#24226

Purpose

Mammalian expression of human E2F2 with HA tag

Depositor

Kristian Helin

Article

Lukas et al Mol Cell Biol. 1996 Mar . 16(3):1047-57.

Insert

E2F2 (E2F2 Human)

Tags

HA

Expression

Mammalian

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pDendra2-H2b

Plasmid

#157817

Purpose

Fluorescent reporter for histone H2B tracking

Depositor

Xin Chen

Article

Ma et al Cell Rep. 2020 Aug 11;32(6):108003. doi:

Insert

Histone H2B (H2bc3 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pEYFP-Mitotrap

Plasmid

#46942

Purpose

Expresses Mitotrap in mammalian cells

Depositor

Margaret Robinson

Article

Robinson et al Dev Cell. 2010 Feb 16;18(2):324-31. doi:

Insert

Mitotrap (MTOR Synthetic, Aequorea victoria, Human, Budding Yeast)

Tags

This is a fusion between a yeast mitochondrial ta…

Expression

Mammalian

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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dCas9-10xSunTag(22aa)

Plasmid

#215745

Purpose

Expresses dCas9 with 10 GCN4 epitopes separated by 22 amino acid linkers

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

dCas9-10xSunTag

Expression

Mammalian

Promoter

chicken beta-actin promoter

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pARF1-CFP

Plasmid

#11381

Depositor

Joel Swanson

Article

Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

Insert

ADP Ribosylation Factor 1 (ARF1 Human)

Tags

CFP

Expression

Mammalian

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only

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pFETCh_RAD21

Plasmid

#64056

Purpose

Homology Arms and 3X Flag with P2A Neo for 3' tagging of human RAD21

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

RAD21 Homology Arms (RAD21 Human)

Tags

3X Flag P2A Neo

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pARF1(T31N)-CFP

Plasmid

#11384

Depositor

Joel Swanson

Article

Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

Insert

ADP Ribosylation Factor 1 (ARF1 Human)

Tags

CFP

Expression

Mammalian

Mutation

Changed Threonine 31 to Asparagine.

Available Since

March 3, 2006

Availability

Academic Institutions and Nonprofits only

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phINA

Plasmid

#132606

Purpose

Expresses human alpha-internexin

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

INA (INA Human)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ExRai-AktAR

Plasmid

#118462

Purpose

cpGFP-based excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-AktAR

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pTO149_RFP_CauNEO

Plasmid

#177277

Purpose

Encodes a G418 (NEOr) selectable marker with codon-optimization for expression in CUG clade fungi. Also encodes RFP tagging of Candida auris ENO1 to measure transformation efficiency.

Depositor

Teresa O'Meara

Article

Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:

Insert

RFP, CauNEO

Tags

ENO1-RFP

Expression

Bacterial

Mutation

All CUG Leucine codons were replaced with the syn…

Promoter

AgTEF2

Available Since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-mFlagEts1

Plasmid

#86099

Purpose

expresses flag tagged Ets1 in mammalian cells

Depositor

Barbara Graves

Article

Seidel et al Genes Dev. 2002 Jan 1;16(1):127-37.

Insert

Ets1 (Ets1 Mouse)

Tags

Flag tag; kanamycin selection

Expression

Mammalian

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pfa6a PhoCl-TsCC(B)::KANMX

Plasmid

#185906

Purpose

Plasmid for cloning PhoCl fused to TsCC(B) into yeast.

Depositor

Matthew Good

Article

Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

Insert

PhoCl-TsCC(B)

Tags

PhoCl fluorophore with C-terminal TsCC(B)

Expression

Yeast

Promoter

SP6

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-GASC1

Plasmid

#24214

Purpose

Mammalian expression of human JMJD2C with HA tag

Depositor

Kristian Helin

Article

Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

Insert

GASC1 (KDM4C Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pLR5-CAG-hMCP-mScarlet-I-NLS

Plasmid

#174878

Purpose

PiggyBac transposon vector for expression of MCP-mScarlet-I-NLS

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

MCP-mScarlet-I-NLS

Use

Piggybac vector

Expression

Mammalian

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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3xFLAG-SENP7

Plasmid

#42886

Depositor

Edward Yeh

Article

Bawa-Khalfe et al Proc Natl Acad Sci U S A. 2012 Oct 23;10

Insert

SENP7 (SENP7 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

T102A, R969C, L996S

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFR-miRFP670nano3

Plasmid

#184673

Purpose

EGF receptor (EGFR) labelled with near-infrared fluorescent protein miRFP670nano3

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

EGFR-miRFP670nano3

Tags

miRFP670nano3

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-Vimentin-7

Plasmid

#55333

Purpose

Localization: Intermediate Filaments, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Vimentin (VIM Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pmCherry_sgRNA-ver2

Plasmid

#126776

Purpose

gRNA expression vector with mCherry transfection control, does not contain the direct repeat of the truncated guideRNA

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

sgRNA (for SpCas9)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pEX-CMV-SP-STIM2(15-746)

Plasmid

#18868

Depositor

Tobias Meyer

Article

Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

Insert

STIM2 (STIM2 Human)

Expression

Mammalian

Mutation

Signal peptide(SP) 1-10 from STIM1 and residues 1…

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
p4B5-EGFP

Plasmid

#140324

Purpose

Detection of Dengue virus infection in live cells

Depositor

Alan Rothman

Article

Medin et al J Virol Methods. 2015 Jan;211:55-62. doi

Insert

DENV2-NGC NS4b5(10 a.a.)-NLS-eGFP

Tags

eGFP

Expression

Mammalian

Mutation

2 conservative n.t. differences near 5' end …

Promoter

CMV

Available Since

May 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-Rab4a-7

Plasmid

#56440

Purpose

Localization: Ras GTPase, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Article

Fang et al Traffic. 2010 Jun;11(6):782-99. doi: 10.

Insert

Rab4a (RAB4A Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pH2B-emiRFP703

Plasmid

#136567

Purpose

Histone 2B labeling with near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

H2B-emiRFP703

Tags

emiRFP703

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG_hPPP1R15B_4-713_mCherry_UK1298

Plasmid

#80707

Purpose

mammalian expression N-term FLAG and C-term mCherry hPPP1R15B (CReP 4-713)

Depositor

David Ron

Article

Chen et al Elife. 2015 Mar 16;4. doi: 10.7554/eLife

Insert

human PPP1R15B (PPP1R15B Human)

Tags

mCherry

Expression

Mammalian

Mutation

CDS 4-713 no stop codon

Promoter

CMV

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pVenus-VSVG

Plasmid

#11914

Depositor

Jennifer Lippincott-Schwartz

Article

Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

Insert

VSVG

Expression

Mammalian

Mutation

F46L, F64L, M153T, V163A, S175G, T203Y, A206K rel…

Available Since

Sept. 13, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
p3xFLAG-CMV10-hUVRAG

Plasmid

#24292

Depositor

Noboru Mizushima

Article

Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

Insert

UV irradiation resistance-associated gene (UVRAG Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-AKAR4-NLS

Plasmid

#138217

Purpose

Nuclear-targeted FRET-based PKA activity reporter.

Depositor

Jin Zhang

Article

Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

Insert

AKAR4-NLS

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
peCALWY-4

Plasmid

#22236

Depositor

Maarten Merkx

Article

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Insert

eCALWY-4 Zn(II) sensor (ATOX1 GFP's, Human)

Expression

Mammalian

Mutation

C418S mutation linker consists of 9 GGSGGS repea…

Available Since

Nov. 25, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-E2F4

Plasmid

#10914

Depositor

Bob Weinberg

Article

Sardet et al Proc Natl Acad Sci U S A. 1995 Mar 14. 9

Insert

E2F4 (E2F4 Human)

Expression

Mammalian

Available Since

Nov. 11, 2005

Availability

Academic Institutions and Nonprofits only
pcKv10.1

Plasmid

#85703

Purpose

mammalian expression Kv10.1

Depositor

Luis Pardo

Article

Chen et al PLoS One. 2011 May 3;6(5):e19257. doi: 1

Insert

KCNH1 (KCNH1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
dClover2-N1

Plasmid

#54538

Purpose

Localization: N1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 A206K, H231L based on publication.

Depositor

Michael Davidson

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Type

Empty backbone

Tags

dClover2

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
hGATA-1 WT

Plasmid

#118352

Purpose

This plasmid expresses human GATA-1 WT.

Depositor

Lea Sistonen

Article

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Insert

hGATA-1 (GATA1 Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

WT

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LYN11-FRB-CFP

Plasmid

#38003

Depositor

Robin Irvine

Article

Hammond et al Science. 2012 Jun 21.

Inserts

LYN (LYN Human)
MTOR (MTOR )

Tags

ECFP

Expression

Mammalian

Available Since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only
pFX-β-tubulin-mCherry

Plasmid

#175829

Purpose

Express mCherry with microtubule-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mCherry with microtuble-targeting sequence

Tags

human tubulin beta 4B class IVb;TUBB4B

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
f-CTS-EGFP

Plasmid

#86064

Purpose

A mammalian expression plasmid encoding fibrocystin ciliary targeting signal domain with C-terminus EGFP.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Fibrocystin (Pkhd1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

contains ciliary targeting signal (3870-3887 amin…

Promoter

CMV

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
IMS-HyPer2

Plasmid

#60248

Purpose

Genetically-encoded fluorescent hydrogen peroxide indicator HyPer2 targeted to mitochondrial intermembrane spase.

Depositor

Vsevolod Belousov

Article

Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

Insert

IMS-HyPer2

Tags

Mitochondrial intermembrane space targeting signa…

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.4_1G11F2-3 heavy chain

Plasmid

#198253

Purpose

recombinant expression of heavy chain of monoclonal anti-AMP antibody 1G11F2-3

Depositor

Aymelt Itzen

Article

Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

Insert

heavy chain of monoclonal anti-AMP antibody 1G11F2-3 (mouse IgG2b)

Tags

IL10 signal peptide

Expression

Mammalian

Promoter

CMV

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C3_SEPT6

Plasmid

#71583

Purpose

mammalian expression of human SEPT6 with a GFP fusion

Depositor

Elias Spiliotis

Article

Hu et al Curr Biol. 2012 Jun 19;22(12):1109-15. d

Insert

Septin 6 (SEPTIN6 Human)

Tags

eGFP

Expression

Mammalian

Mutation

V206D

Promoter

CMV

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
hGip-R

Plasmid

#14942

Depositor

Bernard Thorens

Article

Gremlich et al Diabetes. 1995 Oct . 44(10):1202-8.

Insert

Gip-R (GIPR Human)

Expression

Mammalian

Mutation

Short form of the receptor

Available Since

June 7, 2007

Availability

Academic Institutions and Nonprofits only
pCMV-3XFLAG-renilla-luciferase-beta-globin-control

Plasmid

#184393

Purpose

Transient transfection plasmid expressing Renilla-luciferase-beta-globin fusion protein

Depositor

Robert Bradley

Article

Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

Insert

Renilla luciferase

Tags

3X FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-sapphireCKAR

Plasmid

#118468

Purpose

cp-T-sapphire-based single-color biosensor for monitoring Protein Kinase C activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

sapphireCKAR

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 18,147 results for: Eomes