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19,771 results

Showing: 10251 - 10300 of 19771 results

  12. pET28-SpRY-NLS-6xHis (gbr2101)

    Plasmid
    #181743
    Purpose
    bacterial expression plasmid for SpRY with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag
    Depositor
    Benjamin Kleinstiver
    Article
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Insert
    bacterial codon optimized SpRY
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS(SV40)-6xHis and MKIEE MBP tag
    Expression
    Bacterial
    Mutation
    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…
    Promoter
    T7
    Available Since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    181743-plasmid-map-sequence-id-354675

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  21. p5'Entry-lck-5.5kb

    Plasmid
    #58890
    Purpose
    provides zebrafish lck promoter (5.5 kb) for GateWay construction of Tol2 transgenes
    Depositor
    Jeffrey Yoder
    Insert
    zebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 5.5 kb
    Use
    Gateway 5' entry clone
    Mutation
    mutated single nucleotide (T-->C, 13th nucleot…
    Promoter
    lymphocyte-specific protein tyrosine kinase (lck)…
    Available Since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    58890-plasmid-map-sequence-id-91281

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  29. pRSET-RCaMP1h

    Plasmid
    #42874
    Purpose
    a single-wavelength red-shifted genetically encoded calcium indicator constructed from calmodulin and cp-mRuby
    Depositor
    Loren Looger
    Article
    Akerboom et al Front Mol Neurosci. 2013;6:2. doi: 10.33
    Insert
    Red Fluorescent Calcium binding Protein
    Tags
    EK (Enterokinase) Recognition Site, His Tag (6x),…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    42874-plasmid-map-sequence-id-58020

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  30. p46Cpf1-OP2

    Plasmid
    #98592
    Purpose
    Produces lambda Red and Cpf1 for recombination and selection. The plasmid is combined with a donor plasmid (with crRNA and template) for rapid genome editing in E.coli.
    Depositor
    Qiong Wu
    Article
    Ao et al Front Microbiol. 2018 Oct 9;9:2307. doi:
    Insert
    FnCpf1
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Codon optimized for E. coli
    Promoter
    araBAD
    Available Since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    98592-plasmid-map-sequence-id-201329

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  32. pEGFP-N1-GRASP55

    Plasmid
    #137708
    Purpose
    Eukaryotic expression of rat GRASP55 full-length
    Depositor
    Yanzhuang Wang
    Insert
    GRASP55
    Tags
    EGFP
    Expression
    Mammalian
    Available Since
    March 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    137708-plasmid-map-sequence-id-273547

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Showing: 10251 - 10300 of 19771 results