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We narrowed to 16,985 results for: She

Showing: 10351 - 10400 of 16985 results

  3. pRG01-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-WPRE

    Plasmid
    #123362
    Purpose
    Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance and Firefly luciferase.
    Depositor
    Sidi Chen
    Article
    Chow et al Nat Methods. 2019 May;16(5):405-408. doi
    Insert
    Firefly luciferase
    Use
    CRISPR, Lentiviral, and Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    123362-plasmid-map-sequence-id-240467

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  4. pSOMA-NLS

    Plasmid
    #118937
    Purpose
    Used to express the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thaliana
    Depositor
    Patrick Krysan
    Article
    Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1
    Insert
    SOMA Sensor for Arabidopsis MAPK activity with Nuclear Localization Signal
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    118937-plasmid-map-sequence-id-232109

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  5. p11d-tRPA-32Ala9

    Plasmid
    #102616
    Purpose
    Expression of human RPA with an alanine substituted phosphorylation domain (with the following mutations in RPA32 :S8,11-13,23,29,33,39A,T21A).
    Depositor
    Marc Wold
    Inserts
    RPA1 (RPA1 Human)
    RPA3 (RPA3 )
    RPA2 (RPA2 )
    Use
    Tags
    Expression
    Bacterial
    Mutation
    with an alanine substituted phosphorylation domain
    Promoter
    T7
    Available since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    102616-plasmid-map-sequence-id-197482

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  8. pSOMA-NES

    Plasmid
    #118939
    Purpose
    Used to express the SOMA FRET sensor with a nuclear exclusion signal in Arabidopsis thaliana
    Depositor
    Patrick Krysan
    Article
    Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1
    Insert
    SOMA Sensor for Arabidopsis MAPK activity with Nuclear Exclusion Signal
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    118939-plasmid-map-sequence-id-232111

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  10. pLenti-FoxJ1 promoter-IRES-EGFP-Flag-hChibby1

    Plasmid
    #122964
    Purpose
    Expresses Flag-tagged Chibby1 in multiciliated cells under FoxJ1 promoter. It can be used as a cloning vector for gene of interest at SfiI sites.
    Depositor
    FENG-QIAN Li, Ken-Ichi Takemaru
    Insert
    human Chibby1 (CBY1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mutation
    Promoter
    FoxJ1 and human FoxJ1
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    122964-plasmid-map-sequence-id-291837

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  14. lentiMutB3GALT5catdead-blast

    Plasmid
    #220869
    Purpose
    lentiviral vector for expressing human B3GALT5 with D156A and D243A inactivating mutations and C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence
    Depositor
    Jennifer Kohler
    Insert
    beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
    Use
    Lentiviral
    Tags
    myc, FLAG
    Expression
    Mammalian
    Mutation
    encodes D156A and D243A mutations; also, nucleoti…
    Promoter
    Available since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    220869-plasmid-map-sequence-id-440865

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  17. hP2X3-cALFA

    Plasmid
    #160499
    Purpose
    For the expression of human P2X3 with ALFA tag at C-terminus in mammalian cells
    Depositor
    Motoyuki Hattori
    Article
    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:
    Insert
    human P2X3 (P2RX3 Synthetic, Human)
    Use
    Tags
    HRV3C protease cleavage site-ALFA tag-twin Strep …
    Expression
    Mammalian
    Mutation
    ΔN5ΔC33-T13P/S15V/V16I
    Promoter
    Available since
    Jan. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    160499-plasmid-map-sequence-id-320663

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  21. PCRT7 tetR pLtetO MBP-MEK1 XX Amp

    Plasmid
    #53225
    Purpose
    human MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to amber codons, for use in phosphoprotein synthesis
    Depositor
    Jesse Rinehart
    Insert
    MEK1 (MAP2K1 Human)
    Use
    Phosphoprotein synthesis
    Tags
    HIS and MBP
    Expression
    Bacterial
    Mutation
    Codon optimized for E.coli, both codons for Ser21…
    Promoter
    pLtetO
    Available since
    May 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    53225-plasmid-map-sequence-id-82901

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  23. pUBC_HA_PP7-mVenus

    Plasmid
    #111268
    Purpose
    Transient expression of non-aggregating delta-FG mutant, fused to mVenus in mammalian cells, under a hUBC promoter, HA tag
    Depositor
    John Rinn
    Article
    Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10
    Insert
    PP7-mVenus
    Use
    Synthetic Biology
    Tags
    HA, SV40 NLS, and mVenus
    Expression
    Mammalian
    Mutation
    PP7: C68S, S69G [delta]70-75 (non-aggregating)
    Promoter
    hUBC
    Available since
    June 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    111268-plasmid-map-sequence-id-212603

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  24. PX458_3XHA_den1FnCas9_KRAB_T2A_eGFP

    Plasmid
    #200910
    Purpose
    3XHA tagged dead en1Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression
    Depositor
    Debojyoti Chakraborty
    Insert
    3XHA_den1FnCas9_KRAB_eGFP
    Use
    CRISPR
    Tags
    3xHA-SV40NLS and Nucleoplasmin NLS-KRAB-T2A-EGFP
    Expression
    Mammalian
    Mutation
    Changed Glutamic Acid to Arginine at 1369 position
    Promoter
    CBH
    Available since
    Sept. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    200910-plasmid-map-sequence-id-407164

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  35. pTrc99S-ssDsbA-EPA-DNNNS-DQNRT

    Plasmid
    #128404
    Purpose
    Periplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coli
    Depositor
    Michael Jewett
    Article
    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9
    Insert
    Exotoxin A (toxA P. aeruginosa)
    Use
    Tags
    6xHis tag and DsbA signal sequence for periplasmi…
    Expression
    Bacterial
    Mutation
    242DNNNS246 and 384DQNRT388 engineered glycosylat…
    Promoter
    trc
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    128404-plasmid-map-sequence-id-261690

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  38. pKT-mIDH1(R132H)-Myc-FLAG-IRES-Kat

    Plasmid
    #133779
    Purpose
    Expresses IDH1 with a R132H mutation and has Myc and FLAG tags. IDH1(R132H) also has a katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Maria Castro
    Insert
    Isocitrate dehydrogenase 1 (IDH1 Human)
    Use
    Tags
    Myc and FLAG
    Expression
    Mammalian
    Mutation
    Arginine is mutated to histidine at amino acid po…
    Promoter
    Available since
    Oct. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    133779-plasmid-map-sequence-id-261114

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  39. pUASp YFP Ras85D WT

    Plasmid
    #52242
    Purpose
    Drosophila expression of YFP-Ras85D
    Depositor
    Matthew Scott
    Insert
    Ras85D (Ras85D Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    Promoter
    GAL4
    Available since
    March 20, 2014
    Availability
    Academic Institutions and Nonprofits only

  41. pTrc99S-ssDsbA-TTlight-4xDQNAT

    Plasmid
    #128402
    Purpose
    Periplasmic expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites in E. coli
    Depositor
    Michael Jewett
    Article
    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9
    Insert
    Tetanus toxin light chain domain
    Use
    Tags
    4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…
    Expression
    Bacterial
    Mutation
    Inactivating E234A mutation
    Promoter
    trc
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    128402-plasmid-map-sequence-id-263357

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Showing: 10351 - 10400 of 16985 results