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174,131 results

Showing: 6271 - 6300 of 174131 results

  2. ELONGIN BC (XX01TCEB1A-c001)

    Plasmid
    #110274
    Purpose
    Elongin B and Elongin C co-expression plasmid for structure determination; Full length Elongin B & amino acids 17-112 of Elogin C
    Depositor
    Nicola Burgess-Brown
    Insert
    Elongin BC (ELOC, ELOB Human)
    Expression
    Bacterial
    Mutation
    Elongin B is full length. Elongin C contains a.a…
    Promoter
    T7
    Available Since
    May 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    110274-plasmid-map-sequence-id-209817

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  3. CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))

    Plasmid
    #105842
    Purpose
    Donor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichment
    Depositor
    Michael Ward
    Article
    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    Optimized mApple
    rtTA3G
    SBP Delta-LNGFR
    Use
    CRISPR and TALEN
    Promoter
    CAG, EF-1alpha, and TRE3G
    Available Since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    105842-plasmid-map-sequence-id-393690

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  6. TLCV2-RB1

    Plasmid
    #87836
    Purpose
    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive expression of an sgRNA targeting human RB1.
    Depositor
    Adam Karpf
    Article
    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:
    Insert
    sgRB1 (RB1 Human)
    Use
    CRISPR and Lentiviral; Doxycycline inducible; egf…
    Expression
    Mammalian
    Promoter
    Tight TRE promoter and U6
    Available Since
    April 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    87836-plasmid-map-sequence-id-176310

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  12. pScarlessHD-sfGFP-DsRed

    Plasmid
    #80811
    Purpose
    Vector containing a cassette for scarless insertion of sfGFP and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.
    Depositor
    Kate O'Connor-Giles
    Insert
    sfGFP-piggyBac-DsRed
    Use
    Unspecified
    Tags
    sfGFP
    Available Since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    80811-plasmid-map-sequence-id-152800

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  16. pRubiG-T2A-Cas9

    Plasmid
    #75348
    Purpose
    Ubiquitin promoter expresses GFP and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.
    Depositor
    Bryan Luikart
    Article
    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10
    Insert
    Cas9
    Use
    CRISPR and Retroviral
    Tags
    GFP (via t2a)
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    May 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    75348-plasmid-map-sequence-id-142640

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  18. pdCas9-DNMT3A-PuroR (ANV)

    Plasmid
    #71684
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Synthetic, Human, S. pyogenes)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71684-plasmid-map-sequence-id-129672

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  19. pSS9

    Plasmid
    #71655
    Purpose
    Contains 600 bp homology arms to SS9 for one-step markerless genome integration in E. coli
    Depositor
    Ryan Gill
    Article
    Bassalo et al ACS Synth Biol. 2016 Apr 22.
    Insert
    gfpuv
    Use
    Synthetic Biology
    Promoter
    T7A1
    Available Since
    Jan. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    71655-plasmid-map-sequence-id-257561

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  23. pYLCRISPR/Cas9P35S-B

    Plasmid
    #66190
    Purpose
    expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), basta selection
    Depositor
    Yao-Guang Liu
    Article
    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(
    Insert
    Cas9
    Expression
    Plant
    Mutation
    plant-codon optimized with higher GC contents (62…
    Promoter
    cauliflower mosaic virus 35S promoter (P35S)
    Available Since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    66190-plasmid-map-sequence-id-169495

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  27. mEmerald-Golgi-7

    Plasmid
    #54108
    Purpose
    Localization: Golgi Complex, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Golgi (B4GALT1 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    aa 1-82 of NM_001497.3
    Promoter
    CMV
    Available Since
    July 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54108-plasmid-map-sequence-id-252949

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Showing: 6271 - 6300 of 174131 results