165,224 results

1. pCAG/HA-hBIG2

   Plasmid

   #226254

   Purpose

   Expresses N-terminally HA-tagged hBIG2 in mammalian cells

   Depositor

   Hye-Won Shin

   Insert

   BIG2 (ARFGEF2 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. NSP6-Flag

   Plasmid

   #210345

   Purpose

   Expresses SARS-CoV-2 NSP6 fused with Flag in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   SARS-CoV-2 NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Flag-Tet1 CD

   Plasmid

   #70129

   Purpose

   Express flag-tagged mouse Tet1 catalytic domain.

   Depositor

   Yi Zhang

   Article

   Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

   Insert

   Ten-Eleven Translocation 1 (Tet1 Mouse)
   

   Tags

   flag

   Expression

   Mammalian

   Mutation

   The plasmid expresses TET1 catalytic domain.

   Available Since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG2IG-Hmga1a

   Plasmid

   #122293

   Purpose

   Overexpression for mouse Hmga1a

   Depositor

   Yukiko Gotoh

   Article

   Kishi et al Nat Neurosci. 2012 Aug;15(8):1127-33. do

   Insert

   Hmga1a (Hmga1 Mouse)
   

   Expression

   Mammalian

   Available Since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSBbi 41BBL-CD86

   Plasmid

   #183244

   Purpose

   Expression of human 41BBL and CD86 in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells

   Depositor

   Alexander McLellan

   Article

   Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

   Insert

   CD86, 41BBL
   

   Expression

   Mammalian

   Available Since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIRESneo-FLAG/HA Ago2 corrected

   Plasmid

   #10822

   Depositor

   Thomas Tuschl

   Article

   Meister et al Mol Cell. 2004 Jul 23. 15(2):185-97.

   Insert

   Argonaute 2 (AGO2 Human)
   

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   3 silent mutations in orf

   Available Since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-MRLC1

   Plasmid

   #35680

   Depositor

   Tom Egelhoff

   Article

   Beach et al BMC Cell Biol. 2011 Dec 2;12:52.

   Insert

   MRLC1 (MYL9 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 24, 2012

   Availability

   Academic Institutions and Nonprofits only

8. p3xFLAG CMV14 / BRAF-G469V

   Plasmid

   #131718

   Purpose

   Mammalian Expression of Flag tagged BRAF

   Depositor

   Jacques De Grève

   Article

   Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

   Insert

   BRAF (BRAF Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   G469V

   Promoter

   CMV

   Available Since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST14-ARL3

   Plasmid

   #67376

   Purpose

   Expression of native hArl3 in E. coli

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL3 (ARL3 Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pAAV-TRE-DIO-Cas9

    Plasmid

    #166601

    Purpose

    Encodes Cre-dependent Cas9 under control of the TRE

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    Cas9
    

    Use

    AAV

    Promoter

    tetracycline response element

    Available Since

    April 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA4/TO mTRPM7

    Plasmid

    #45482

    Depositor

    Andrew Scharenberg

    Article

    Nadler et al Nature. 2001 May 31;411(6837):590-5.

    Insert

    TRPM7 (Trpm7 Mouse)
    

    Use

    Tetracycline inducible

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    Tetracycline-controlled CMV

    Available Since

    May 21, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pEntr_Myc-BirA-G3-ER

    Plasmid

    #173814

    Purpose

    Promiscuous biotin ligase (BirA-G3) targeted to ER lumen in Gateway entry vector. N-terminal secretion signal from Drosophila BiP. C-terminal KDEL. Myc tag inserted after sig. seq. cleavage site

    Depositor

    Norbert Perrimon

    Article

    Droujinine et al Nat Commun. 2021 Apr 22;12(1):2382. doi:

    Insert

    Myc-BirA-G3-ER
    

    Use

    Gateway shuttle vector

    Tags

    Myc

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. dPspCas13b-wtFTO

    Plasmid

    #223696

    Purpose

    Expressing fusion protein of dPspCas13b and wild type human FTO

    Depositor

    Chuan He

    Article

    Wei et al Science. 2022 May 27;376(6596):968-973.

    Insert

    dPspCas13b-GGS-wtFTO (FTO Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-pGK-Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro

    Plasmid

    #115684

    Purpose

    NanoLuc-Firefly Luciferase fusion with constitutive nuclear expression for calibration of Nanoluc signal to Firefly Luciferase signal. H2B-mCherry marks chromatin with red fluorescence.

    Depositor

    David Suter

    Article

    Mandic et al Methods. 2017 May 1;120:65-75. doi: 10.1

    Insert

    Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro
    

    Use

    Lentiviral

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLentiCRISPRv2_cGAS_gRNA_3

    Plasmid

    #235531

    Purpose

    gRNA against human cGAS

    Depositor

    Nicolas Manel

    Article

    Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

    Insert

    cGAS (CGAS Human)
    

    Use

    Lentiviral

    Available Since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGMC00030 (aka pRC0707)

    Plasmid

    #209673

    Purpose

    Lentiviral vector expressing PP7-P65-HSF1

    Depositor

    Raj Chari

    Article

    Yang et al Sci Adv. 2024 Feb 16;10(7):eadj2445. doi

    Insert

    PP7-P65-HSF1 (HSF1, RELA Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRG/III-hs-MBP-CB2-HF

    Plasmid

    #121682

    Purpose

    Bacterial expression of cannabinoid receptor CB2 fusion protein

    Depositor

    Richard Henderson, Reinhard Grisshammer

    Article

    Calandra et al Biotechnology Letters

    Insert

    peripheral cannabinoid receptor (CNR2 Human)
    

    Tags

    His6 Flag tag

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#3

    Plasmid

    #172985

    Purpose

    CRISPRi for TAOK2

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    sgTAOK2i (TAOK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEN-sacB

    Plasmid

    #196249

    Purpose

    Plasmid containing the sacB gene

    Depositor

    Bing Yang

    Article

    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

    Insert

    attR2-sacB-attL3
    

    Expression

    Bacterial

    Promoter

    sacB promoter

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. p663-UBC-miniTurbo-V5-CDK20_IDG-K

    Plasmid

    #189883

    Purpose

    Gateway destination clone of CDK20 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    CDK20 (CDK20 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. Cas9-GFP_sg_mAMPKa1

    Plasmid

    #79004

    Purpose

    Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 1.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    Prkaa1 (Prkaa1 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

22. pUC19_HDRT_TRAC_CD19-CAR

    Plasmid

    #183473

    Purpose

    Encodes for a homology-directed DNA repair template for the insertion of a second generation CD19-specific CAR into the TRAC gene

    Depositor

    Dimitrios Wagner

    Article

    Kath et al Mol Ther Methods Clin Dev. 2022 Apr 12;2

    Inserts

    TRAC (TRAC Human)
    CD19-specific CAR transgene insert
    TRAC (TRAC Human)
    

    Use

    Unspecified

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. Xla.Tubb2:hArr-TEV;he1.1:eYFP

    Plasmid

    #218859

    Purpose

    Xla.Tubb2 promoter driving expression of human Arrestin-TEV protease fusion protein for use in trans-Tango.

    Depositor

    Marnie Halpern

    Article

    Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

    Insert

    hArr
    

    Tags

    human Arrestin-Tobbaco Etch Virus protease fusion…

    Expression

    Bacterial

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0

    Plasmid

    #174081

    Purpose

    Expression of orthogonal Mb tRNA and tRNA synthetase for incorporation of tetrazine3.0 in eukaryotic cells.

    Depositor

    Ryan Mehl

    Article

    Jang et al J Am Chem Soc. 2020 Apr 22;142(16):7245-

    Insert

    NES-Flag-R2-84 M. barkeri tRNA synthetase for tetrazine3.0
    

    Tags

    NES-Flag

    Expression

    Mammalian

    Mutation

    L270G, Y271L, N311G, C313A

    Promoter

    CMV

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3puro-EGFP-GAPDH

    Plasmid

    #225475

    Purpose

    Express mEGFP-fusion protein of GAPDH

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    GAPDH (GAPDH Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHTN-HaloTag_DCAF1:1-1507

    Plasmid

    #210972

    Purpose

    Mammalian expression of full-length human DCAF1. N-terminal HaloTag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    DCAF1:M1-E1507 (DCAF1 Human)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available Since

    Feb. 21, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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27. PB_TetON_mCherry_P2AT2A_GFP-nb-KS

    Plasmid

    #238240

    Purpose

    For integration of mCherry_P2AT2A_GFP-nb-KS

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry_P2AT2A_GFP-nb-KS
    

    Expression

    Mammalian

    Available Since

    June 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLenti-EFS-MCS-WPRE

    Plasmid

    #163362

    Purpose

    The empty pLenti backbone with multiple cloning site that allows shuttling between pMY and pMX vectors

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAGGS_cAMPFIRE-M

    Plasmid

    #182284

    Purpose

    Mammalian expression of the cAMP sensor cAMPFIRE-M under a CAG promotor w/ WPRE translation enhancer.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-M (cAMP sensor)
    

    Expression

    Mammalian

    Available Since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pUBC_stdMCP_serinemod_E488QADAR_p2A_yGFP

    Plasmid

    #154787

    Purpose

    Expresses synonymized MS2 coat protein dimer (MCP) fused to hyperactive ADAR catalytic domain

    Depositor

    Robert Singer

    Article

    Biswas et al iScience. 2020 Jun 28;23(7):101318. doi:

    Insert

    Adenosine deaminse (Adar Fly, Synthetic)
    

    Use

    Lentiviral

    Tags

    GFP, V5, and stdMCP

    Expression

    Mammalian

    Mutation

    Synonymized serine to prevent autoediting of ADAR…

    Promoter

    UBC

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. HA-Beclin 1

    Plasmid

    #86748

    Purpose

    N-terminal HA-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

    Insert

    human Beclin 1 (BECN1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTetOFF SNCA-A53T

    Plasmid

    #215372

    Purpose

    All-in-one lentiviral construct for the dox repressible expression of the A53T mutant of alpha-synuclein.

    Depositor

    Franz-Ulrich Hartl

    Article

    Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

    Insert

    SNCA (SNCA Human)
    

    Expression

    Mammalian

    Mutation

    Ala53Thr

    Available Since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSMT3_SRSF1

    Plasmid

    #201056

    Purpose

    Express sumo-tagged human SRSF1

    Depositor

    Jun Zhang

    Article

    Fargason et al Elife. 2023 Mar 2;12:e84412. doi: 10.755

    Insert

    SRSF1 (SRSF1 Human)
    

    Tags

    sumo

    Expression

    Bacterial

    Mutation

    none

    Available Since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHAGE-CTCF

    Plasmid

    #116728

    Purpose

    Lentiviral expression of CTCF

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    CTCF (CTCF Human)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. PiggyBac-TetO-En1-t2a-Foxa2-hygro

    Plasmid

    #176483

    Purpose

    TetO-En1-t2a-Foxa2 in a PiggyBac vector with hygromycin resistance

    Depositor

    Marius Wernig

    Article

    Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

    Insert

    En1-t2a-FoxA2 (FOXA2, En1 Mouse, Human)
    

    Expression

    Mammalian

    Promoter

    TetO

    Available Since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCMV-SpdCas9-VP64

    Plasmid

    #115794

    Purpose

    Expresses SpdCas9-Vp64 fusion protein when packaged into AAV

    Depositor

    Nadav Ahituv

    Article

    Matharu et al Science. 2018 Dec 13. pii: science.aau06

    Insert

    VP64
    

    Use

    AAV

    Tags

    3XFLAG and dCas9-VP64

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEGFP(N3)-PP1beta

    Plasmid

    #44223

    Depositor

    Angus Lamond, Laura Trinkle-Mulcahy

    Article

    Trinkle-Mulcahy et al J Cell Sci. 2001 Dec;114(Pt 23):4219-28.

    Insert

    PP1B (PPP1CB Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. bactin2:loxP-STOP-loxP-DsRed-express

    Plasmid

    #68797

    Purpose

    For making transgenic fish

    Depositor

    David Traver

    Article

    Bertrand et al Nature. 2010 Mar 4;464(7285):108-11. doi

    Inserts

    beta-actin promoter
    loxp-stop-codon-loxp-dsred
    

    Available Since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. GFP-rab11 DN

    Plasmid

    #12678

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB11A (Homo sapiens) (RAB11A Human)
    

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    changed serine 25 to asparagine

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTALYM4SpMi-05

    Plasmid

    #47880

    Purpose

    TALE-mRuby2 against Mus spretus/musculus minor satellite sequence

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

    Insert

    TALE-mRuby2
    

    Use

    TALEN

    Tags

    mRuby2

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pMpGWB337

    Plasmid

    #68061

    Purpose

    Gateway-based vector for Marchantia polymorpha to express a floxed gene, with an expression cassette for Cre-glucocorticoid receptor fusion protein under the control of heat-inducible promoter

    Depositor

    Takayuki Kohchi

    Article

    Nishihama et al Plant Cell Physiol. 2015 Jul 6. pii: pcv

    Type

    Empty backbone

    Use

    Cre/Lox

    Expression

    Plant

    Promoter

    MpEF1alpha promoter

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pJYS1Peftu

    Plasmid

    #85546

    Purpose

    Constitutive expression of FnCpf1 and recT in C.glutamitum, etfu promoter

    Depositor

    Sheng Yang

    Article

    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

    Inserts

    Cpf1
    recT
    

    Use

    CRISPR; Shuttle vector corynebacterium glutamicum…

    Expression

    Bacterial

    Promoter

    etfu and unknown

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. mVenus C1

    Plasmid

    #27794

    Depositor

    Steven Vogel

    Article

    Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

    Has Service

    Cloning Grade DNA

    Insert

    mVenus C1
    

    Expression

    Mammalian

    Available Since

    April 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. BM00654

    Plasmid

    #155339

    Purpose

    Binary vector for expression of PULSE system (under the control of CaMV35S promoter) controlling FLuc. It constitutively expresses RLuc and a plant selection cassette.

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    RB_KanR_Tnos-NLS-PIF6-E-P35S_Tnos-RLuc-PUbi10_Tnos-NLS-VP16-PhyB-P35S_Tnos-EL222-NLS-SRDX-P35S_T35S-FLuc-POpto_LB
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Plant

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pHCMV_SFV_E1E2

    Plasmid

    #207308

    Purpose

    Mammalian expression of Semliki Forest virus E1E2 protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    SFV_E1E2
    

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. CMV-SEAP

    Plasmid

    #24595

    Depositor

    Alan Cochrane

    Insert

    SEAP (ALPP Human)
    

    Expression

    Mammalian

    Available Since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pM-Cas9

    Plasmid

    #196325

    Purpose

    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding SpCas9 in place of viral GP

    Depositor

    Zhenghe Li

    Article

    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

    Insert

    Full length TSWV M antigenome encoding SpCas9 in place of viral GP
    

    Use

    CRISPR

    Tags

    3×flag

    Expression

    Plant

    Mutation

    See depositor comments below

    Promoter

    duplicated cauliflower mosaic virus 35S promoter

    Available Since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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48. pLVX-RNaseHI-NES-EGFP

    Plasmid

    #196701

    Purpose

    Plasmid for stable expression of wild type bacterial RNase HI tagged with NES and EGFP that can be used to specifically degrade the DNA-RNA hybrids in the cytoplasm.

    Depositor

    Karlene Cimprich

    Article

    Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

    Insert

    bacterial RNaseHI
    

    Use

    Lentiviral

    Available Since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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49. pAAV-EF1a-FLEX(frt)-GCaMP6f-WPRE

    Plasmid

    #118273

    Purpose

    pAAV vector for flippase dependent GCaMP6f expression under the control of EF1a promoter

    Depositor

    Naoshige Uchida

    Article

    Menegas et al Nat Neurosci. 2018 Sep 3. pii: 10.1038/s

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    6xHis (N terminal on insert), T7 epitope, and Xpr…

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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50. mCerulean3-C1

    Plasmid

    #54605

    Purpose

    Localization: C1 Cloning Vector, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Type

    Empty backbone

    Tags

    mCerulean3

    Expression

    Mammalian

    Available Since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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