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We narrowed to 13,583 results for: TIM

Showing: 10501 - 10550 of 13583 results

  5. actc1b-2PH-PLCd-mKate2

    Plasmid
    #109500
    Purpose
    Muscle specific zebrafish expression of mKate2 fused to 2x the PH domain from Human phospholipase C delta 1 (PLCD1, codon optimised). Binds PtdIns(4,5)P2.
    Depositor
    Rob Parton
    Article
    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:
    Insert
    actc1b-2PH-PLCd-mKate2
    Use
    Zebrafish plasmids
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    109500-plasmid-map-sequence-id-219972

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  7. pSCMV-H2B-FT-Slow

    Plasmid
    #157671
    Purpose
    Retroviral vector for expressing chimeric human histone H2B fused with the "Slow" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.
    Depositor
    Shangqin Guo
    Article
    Eastman et al Cell Rep. 2020 Jun 23;31(12):107804. doi
    Insert
    Histone H2B
    Use
    Retroviral
    Tags
    Slow-FT
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    157671-plasmid-map-sequence-id-311902

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  8. pSCMV-H2B-FT-Fast

    Plasmid
    #157669
    Purpose
    Retroviral vector for expressing chimeric human histone H2B fused with the "Fast" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.
    Depositor
    Shangqin Guo
    Article
    Eastman et al Cell Rep. 2020 Jun 23;31(12):107804. doi
    Insert
    Histone H2B
    Use
    Retroviral
    Tags
    Fast-FT
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    157669-plasmid-map-sequence-id-308111

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  12. p-mCherry-C1-GtACR1

    Plasmid
    #85464
    Purpose
    Natural anion conducting Channelrhodopsin with cyan absorption. Closing kinetics 140ms. High conductivity. Codon optimized for mammalian expression (human/mouse)
    Depositor
    Peter Hegemann
    Article
    Wietek et al J Biol Chem. 2016 Feb 19;291(8):4121-7.
    Insert
    Synthetic construct ACR1 gene
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV (+enhancer)
    Available since
    Jan. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    85464-plasmid-map-sequence-id-170772

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  15. p-mCherry-C1-GtACR2

    Plasmid
    #85463
    Purpose
    Natural anion conducting Channelrhodopsin with blue absorption. Closing kinetics 60ms. High conductivity. Codon optimized for mammalian expression (human/mouse)
    Depositor
    Peter Hegemann
    Article
    Wietek et al J Biol Chem. 2016 Feb 19;291(8):4121-7.
    Insert
    Synthetic construct ACR2 gene
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV (+enhancer)
    Available since
    Jan. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    85463-plasmid-map-sequence-id-170777

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  25. pMesh1

    Plasmid
    #175594
    Purpose
    Lac inducible, AMP Resistant, non labelled gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.
    Depositor
    Greg Bokinsky
    Article
    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.
    Insert
    Mesh1 (Mesh1 Fly)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Codon optimized for translation in E.coli. AGGAGG…
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    175594-plasmid-map-sequence-id-348364

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  27. His-NavMs

    Plasmid
    #100004
    Purpose
    Electrophysiology in mammalian cells: Mammalian expression of voltage gated sodium channel and GFP
    Depositor
    Paul DeCaen
    Article
    Sula et al Nat Commun. 2017 Feb 16;8:14205. doi: 10
    Insert
    His-NavMs, Voltage gated sodium channel
    Use
    Tags
    Hexa-His tag followed by a thrombin cleavage site
    Expression
    Mammalian
    Mutation
    C-terminus from a codon-optimized synthetic gene,…
    Promoter
    CMV
    Available since
    Aug. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    100004-plasmid-map-sequence-id-192452

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  28. MSP1673

    Plasmid
    #65769
    Purpose
    Bacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
    Use
    CRISPR
    Tags
    NLS
    Expression
    Bacterial
    Mutation
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65769-plasmid-map-sequence-id-112659

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  29. pAcCas12nHS_RV6_empty

    Plasmid
    #203811
    Purpose
    Plasmid containing a CMV-driven NLS-AcCas12n-NLS (human-codonoptimized) cassette and a U6-driven sgRNA_V6 cassette (containing BsaI sites for spacer insertion). Carbenicillin resistance.
    Depositor
    Quanjiang Ji
    Article
    Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046
    Insert
    AcCas12nHS_RV6
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    203811-plasmid-map-sequence-id-405075

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  30. 3xFLAG-dCas9/pMSCVpuro

    Plasmid
    #134983
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    April 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    134983-plasmid-map-sequence-id-430623

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  40. pUB-Cas9

    Plasmid
    #86556
    Purpose
    For ubiquitous expression of Cas9 in planta. sgRNA can be introduced as described in Hahn et al. (2017)
    Depositor
    Andreas Weber
    Article
    Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:
    Insert
    Cas9
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Cas9 is codon optimized for C. reinhardtii
    Promoter
    UBIQUITIN10
    Available since
    May 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86556-plasmid-map-sequence-id-176429

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  48. p5E-pth2

    Plasmid
    #191428
    Purpose
    Tol2 Gateway 5' entry vector containing 2067 bp upstream of the CDS for zebrafish pth2 (ENSDARG00000022951), including the 5' UTR and first intron (GRCz11 chr 17:33493999-33496066)
    Depositor
    Timothy Erickson
    Article
    Venuto et al Biol Open. 2022 Sep 26. pii: 276788. doi
    Insert
    pth2 regulatory region (pth2 Zebrafish)
    Use
    Tol2 gateway 5' entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    191428-plasmid-map-sequence-id-384465

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Showing: 10501 - 10550 of 13583 results