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174,131 results

Showing: 6481 - 6510 of 174131 results

  10. pIVTRup

    Plasmid
    #101362
    Purpose
    Highly efficient in vitro transcription. Empty backbone.
    Depositor
    Ángel Raya
    Insert
    5'UTR-EcoRV site-3'UTR-pAtrack(55 pA)
    Use
    For cloning a gene for in vitro transcription pur…
    Promoter
    T7
    Available Since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    101362-plasmid-map-sequence-id-352564

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  11. pDG462

    Plasmid
    #100903
    Purpose
    SpCas9n (D10A Nickase mutant) with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available Since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100903-plasmid-map-sequence-id-196607

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  13. eSpCas9(1.1)-T2A-Puro

    Plasmid
    #101039
    Purpose
    Enhanced SpCas9(1.1) with puromycin resistance gene via T2A linker.
    Depositor
    Andrea Németh
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    3xFLAG and T2A-Puro
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    Nov. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    101039-plasmid-map-sequence-id-190363

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  16. pSAM_AraC

    Plasmid
    #91569
    Purpose
    Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.
    Depositor
    Harry Mobley
    Article
    Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.
    Insert
    AraC/PBAD
    Expression
    Bacterial
    Available Since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    91569-plasmid-map-sequence-id-181631

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  23. Lenti-AsCpf1-Blast

    Plasmid
    #84750
    Purpose
    Expresses human codon-optimized AsCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n
    Insert
    AsCpf1
    Use
    CRISPR and Lentiviral
    Tags
    NLS-3xHA (C terminal on insert)
    Expression
    Mammalian
    Promoter
    EFS-NS
    Available Since
    Dec. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    84750-plasmid-map-sequence-id-164466

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  30. DT960

    Plasmid
    #80412
    Purpose
    Expresses 960 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats
    Depositor
    Thomas Cooper
    Article
    Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub
    Insert
    human DMPK exons 11-15 with 960 interrupted CTG repeats (DMPK Human)
    Expression
    Mammalian
    Available Since
    Sept. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    80412-plasmid-map-sequence-id-151397

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Showing: 6481 - 6510 of 174131 results