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171,035 results

Showing: 10801 - 10850 of 171035 results

  1. pN251

    Plasmid
    #188471
    Purpose
    Lentiviral construct encoding doxycycline-inducible enCas12a-HF (via TetON3G)
    Depositor
    Le Cong
    Article
    Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2
    Insert
    enCas12a-HF (gift from Dr. Keith Joung)
    Use
    Lentiviral
    Expression
    Bacterial and Mammalian
    Available Since
    Sept. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    188471-plasmid-map-sequence-id-370973

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  12. gCBEv2

    Plasmid
    #220617
    Purpose
    vector for encoding an engineered glycosylase-based cytosine (C) base editor (gCBEv2) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Insert
    hUNG2-nSpCas9
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;N213D,K184A,A214V), SpCas9(D10A)
    Promoter
    hU6, EF1a
    Available Since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220617-plasmid-map-sequence-id-436955

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  16. pUBC-SV40NLS-PfV-Sapphire-IRES-H2B-mCherry

    Plasmid
    #203652
    Purpose
    Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a secondary translation initiation site for H2B-mCherry expression.
    Depositor
    Liam Holt
    Article
    Shu T et et al bioRxiv 2022
    Insert
    PfV
    Use
    Lentiviral
    Tags
    Sapphire-IRES-H2B-mCherry
    Available Since
    July 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    203652-plasmid-map-sequence-id-402483

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  17. pLKO.3G_X7

    Plasmid
    #171213
    Purpose
    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses eGFP for selection. Please see cloning information for suggested restriction sites
    Depositor
    Jenny Wang
    Insert
    N/A; empty backbone
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Available Since
    July 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    171213-plasmid-map-sequence-id-338282

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  19. pETM33_Nsp16

    Plasmid
    #156482
    Purpose
    Bacterial expression of Sars-CoV2 Nsp16 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp16 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156482-plasmid-map-sequence-id-307736

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  23. plex_306-ccdB-hygro

    Plasmid
    #201135
    Purpose
    pLEX_306 plasmid with hygro marker
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    June 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    201135-plasmid-map-sequence-id-396789

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  27. MultiMate-Rainbow

    Plasmid
    #206252
    Purpose
    Expression of 7 fluorescently labelled proteins in mammalian cells. Can be used to generate recombinant baculovirus particles.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    H2B (human); Actin, Tubulin (B. taurus); mito mCherry, GST mTagBFP1 (Synthetic); CyOFP1 (E. quadricolor); Ctnnb1 (mouse)
    Use
    Recombinant baculovirus production (bac-to-bac)
    Tags
    iRFP713
    Expression
    Mammalian
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206252-plasmid-map-sequence-id-406651

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  33. pAAV-CAG-DIO-HA-HCARD-P2A-mCitrine

    Plasmid
    #228902
    Purpose
    For AAV packaging and Cre-dependent expression of a peripherally restricted DREADD
    Depositor
    Bryan Roth
    Article
    Kang et al Cell. 2024 Dec 26;187(26):7433-7449.e20.
    Has Service
    AAV8
    Insert
    hydroxycarboxylic acid receptor 2 (Hcar2 Mouse)
    Use
    AAV and Synthetic Biology
    Tags
    HA and P2A mCitrine
    Expression
    Mammalian
    Mutation
    R108K
    Promoter
    Chicken Beta Actin
    Available Since
    Dec. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    228902-plasmid-map-sequence-id-454100

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  35. DRD2-Tango

    Plasmid
    #66269
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    DRD2 (DRD2 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66269-plasmid-map-sequence-id-157899

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  39. BRD3

    Plasmid
    #38940
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BRD3 (BRD3 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  40. pBabe puro IRES-EGFP

    Plasmid
    #14430
    Depositor
    L. Miguel Martins
    Has Service
    Cloning Grade DNA
    Insert
    IRES-EGFP
    Use
    Retroviral
    Expression
    Mammalian
    Available Since
    March 16, 2007
    Availability
    Academic Institutions and Nonprofits only
    14430-plasmid-map-sequence-id-251302

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  41. pET28a hMDH1v3 (+TEV)

    Plasmid
    #204229
    Purpose
    Bacterial expression of human cytosolic malate dehydrogenase isoform 1 transcript variant 3 (hMDH1v3); Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    hMDH1v3 (MDH1 Human)
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Inserted a G after ATG to maintain kozak site
    Promoter
    Lac
    Available Since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204229-plasmid-map-sequence-id-413334

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  43. pRL1383a

    Plasmid
    #70692
    Purpose
    RSF1010-based vector with extensive polylinker, and resistance to Strep and Spec. Shuttle vector suitable for transfer to, and replication in, E. coli and Anabaena (Nostoc) sp. strain PCC 7120.
    Depositor
    Peter Wolk
    Article
    Wolk et al Arch Microbiol. 2007 Dec;188(6):551-63.
    Type
    Empty backbone
    Use
    Bacterial shuttle vector
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    70692-plasmid-map-sequence-id-126476

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Showing: 10801 - 10850 of 171035 results