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173,273 results

Showing: 10801 - 10850 of 173273 results

  8. pGL6000

    Plasmid
    #216797
    Purpose
    Platform for DiCre inducible expression of a gene of interest in Leishmania major - requires Leishmania strain expressing DiCre recombinase
    Depositor
    Jeremy Mottram
    Article
    Santos et al Mol Biochem Parasitol. 2017 Sep;216:45-4
    Type
    Empty backbone
    Use
    Cre/Lox; Leishmania dicre inducible expression
    Tags
    6xHA
    Promoter
    rRNA promoter
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    216797-plasmid-map-sequence-id-433103

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  9. MRGPRX4-DuET

    Plasmid
    #213347
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    MRGPRX4 (MRGPRX4 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    213347-plasmid-map-sequence-id-431825

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  10. MRGPRG-DuET

    Plasmid
    #213343
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    MRGPRG (MRGPRG Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213343-plasmid-map-sequence-id-430871

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  22. PX459v3

    Plasmid
    #178799
    Purpose
    SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available Since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178799-plasmid-map-sequence-id-354842

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  26. p4xSTAT6-Luc2P

    Plasmid
    #35554
    Depositor
    Axel Nohturfft
    Insert
    4xStat6 promoter
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    4xStat6
    Available Since
    March 29, 2012
    Availability
    Academic Institutions and Nonprofits only
    35554-plasmid-map-sequence-id-338744

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  28. plex_307-ccdB-blasticidin

    Plasmid
    #201136
    Purpose
    pLEX_307 plasmid with blasticidin marker
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    EF-1α
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    201136-plasmid-map-sequence-id-395507

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  39. Cas12aUltra_5XNLS

    Plasmid
    #218775
    Purpose
    His-TEV-Cas12aUltra-BPSV40_cMyc_NP_5NLS
    Depositor
    Ross Wilson
    Article
    Sahu et al Nat Protoc. 2025 Mar 3. doi: 10.1038/s41
    Insert
    5xNLS-BPSV40-NLP-cMyc-cMyc-BPSV40- AspCas12a
    Use
    CRISPR
    Tags
    6XHis (C terminal), HA, Nucleoplasmin, SV40, and …
    Expression
    Bacterial
    Mutation
    Nucleotides mutations were made to encode for arg…
    Available Since
    Aug. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    218775-plasmid-map-sequence-id-443448

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  42. pGEX-GST-BcPC-PLC

    Plasmid
    #224255
    Purpose
    Express N-terminal HRV 3C protease cleavable GST fused to Bacillus cereus PC-PLC in E.coli
    Depositor
    Chiaki Murakami
    Article
    Murakami et al Anal Biochem. 2017 Jun 1;526:43-49. doi:
    Insert
    Bacillus cereus phospholipase C (plC, plC Bacillus cereus ATCC 14579)
    Tags
    GST and HRV-3C (LEVLFQGP) site
    Expression
    Bacterial
    Mutation
    The gene of Bacillus cereus ATCC 14579 was used f…
    Promoter
    lac promoter
    Available Since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    224255-plasmid-map-sequence-id-457146

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  48. pDV044

    Plasmid
    #191824
    Purpose
    Plasmid for the transformation of the cyanobacterium Arthrospira platensis at the KmR locus. pCPC600 promoter. With terminator. Golden Gate compatible. Selection: in A. platensis: KmR; E. coli: AmpR
    Depositor
    Jim Roberts
    Article
    Tabakh et al STAR Protoc. 2023 Feb 8;4(1):102087. doi
    Type
    Empty backbone
    Use
    Unspecified
    Available Since
    Jan. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    191824-plasmid-map-sequence-id-375877

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Showing: 10801 - 10850 of 173273 results