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Showing: 8681 - 8720 of 157401 results

  4. AICSDP-166:CDH1-mEGFP

    Plasmid
    #193921
    Purpose
    Homology arms and LE-mEGFP sequence for C-terminus tagging of human cadherin 1
    Depositor
    Allen Institute for Cell Science
    Insert
    cadherin 1 Homology Arms with LE-mEGFP (CDH1 Human)
    Use
    AAV and CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Dec. 22, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    193921-plasmid-map-sequence-id-380537

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  6. pIS1

    Plasmid
    #12179
    Depositor
    David Bartel
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    12179-plasmid-map-sequence-id-234698

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  8. EBFP2-H2B-6

    Plasmid
    #55243
    Purpose
    Localization: Nucleus/Histones, Excitation: 383, Emission: 448
    Depositor
    Michael Davidson
    Insert
    H2B (H2BC11 Human)
    Use
    Tags
    EBFP2
    Expression
    Mammalian
    Mutation
    D26G and V119I in H2B
    Promoter
    CMV
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    55243-plasmid-map-sequence-id-159436

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  9. Col1A2:TFPnls-T2a-mErt-Cre-Ert

    Plasmid
    #111151
    Purpose
    Fibroblasts specific inducible Cre
    Depositor
    Elly Tanaka
    Insert
    TFPnls-T2a-mErt-Cre-Ert
    Use
    Cre/Lox
    Tags
    Expression
    Mutation
    wt
    Promoter
    Col1A2
    Available since
    June 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    111151-plasmid-map-sequence-id-211534

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  15. Salsa6f

    Plasmid
    #140188
    Purpose
    Salsa6f is a fluorescent ratiometric genetically encoded calcium indicator, (tdTomato linked to GCaMP6f by a V5 epitope tag)
    Depositor
    Michael Cahalan
    Article
    Dong et al eLife. 2017 Dec 14;6. pii: 32417. doi: 1
    Insert
    Salsa6f
    Use
    Tags
    tdTomato-v5-GCaMP6f
    Expression
    Mammalian
    Mutation
    Promoter
    CMV Immediate Early
    Available since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    140188-plasmid-map-sequence-id-316791

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  18. pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40

    Plasmid
    #100054
    Purpose
    AAV expression of humanized ChR2 with H134R mutation fused to mCherry driven by CAG promoter for optogenetic activation
    Depositor
    Karl Deisseroth
    Has Service
    AAV1, AAV2, AAV5, and AAV9
    Insert
    channelrhodopsin-2
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    H134R mutation in ChR2
    Promoter
    CAG
    Available since
    Jan. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    100054-plasmid-map-sequence-id-183171

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  20. mEmerald-CLIP170-N-18

    Plasmid
    #54044
    Purpose
    Localization: Microtubule Binding , Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    CLIP170 (CLIP1 Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54044-plasmid-map-sequence-id-139284

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  22. pcDNA3-Clover

    Plasmid
    #40259
    Purpose
    GFP with robust performance in protein fusions and useful as FRET acceptor for mRuby2 in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap
    Depositor
    Michael Lin
    Article
    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm
    Has Service
    Cloning Grade DNA
    Insert
    Clover GFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV IE
    Available since
    Sept. 24, 2012
    Availability
    Academic Institutions and Nonprofits only
    40259-plasmid-map-sequence-id-215680

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  23. pET22b_PstS_1

    Plasmid
    #78198
    Purpose
    Expression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor
    Depositor
    Martin Webb
    Article
    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62
    Insert
    pstS (A197C)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    A197C (please see depositors comment below)
    Promoter
    T7
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    78198-plasmid-map-sequence-id-146048

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  26. TDP-REGv2(Gaussia Luciferase)#5 in pTwist-CMV

    Plasmid
    #216164
    Purpose
    Expression of secreted Gaussia Luciferase specifically in cells with TDP-43 loss of function. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    Gaussia princeps luciferase (with double mutation to improve stability)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216164-plasmid-map-sequence-id-437759

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  30. pGL3- sgAAVS1.5-CAG-Cas9-T2A-mCherry-P2A-Puro

    Plasmid
    #216246
    Purpose
    Express Cas9 with CAG promoter to improve the expression in hES cells and a highly efficient sgAAVS1.5 for AAVS1 integration
    Depositor
    Elina Ikonen
    Article
    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:
    Insert
    Cas9 and sgAAVS1.5
    Use
    CRISPR; Human safe harbor locus (a avs1) site-spe…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    216246-plasmid-map-sequence-id-426842

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  31. TFORF0626

    Plasmid
    #142279
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CEBPZ (CEBPZ Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    142279-plasmid-map-sequence-id-427298

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  35. pET28-3'TwStrep

    Plasmid
    #208053
    Purpose
    Empty vector for prokaryotic expression, insert a Twin Strep tag (with TAA) between HindII and XhoI site of pET28a (+), both HindIII and XhoI are retained. HindIII in-frame with Twin Strep tag.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Tags
    Twin Strep
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208053-plasmid-map-sequence-id-415923

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Showing: 8681 - 8720 of 157401 results