161,202 results

1. pMOS012: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)

   Plasmid

   #163057

   Purpose

   Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   Grx1-roGFP1-iEER
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPD543 ZF21x6-Compact_EYFP

   Plasmid

   #138857

   Purpose

   EYFP reporter vector for the ZF21x6-Compact promoter

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   ZF21x6-Compact
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   ZF21x6-Compact

   Available since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPD528 VP16-ZF21

   Plasmid

   #138757

   Purpose

   Constitutive expression of VP16-ZF21 under the CMV promoter.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   VP16-ZF21
   

   Use

   Synthetic Biology

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBAD24-sfGFPx1

   Plasmid

   #51558

   Purpose

   Superfolder GFP ORF cloned into pBAD24 for expression in E. coli

   Depositor

   Sankar Adhya, Francisco Malagon

   Article

   Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

   Insert

   superfolder GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUb-Cas9-mCherry_cU6:6-sgRNA

   Plasmid

   #190598

   Purpose

   The plasmid encodes S. pyogenes Cas9 and mCherry separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter

   Depositor

   Claudia Rueckert

   Article

   Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.

   Inserts

   Cas9
   sgRNA
   

   Use

   CRISPR

   Tags

   mCherry - separated by T2A

   Expression

   Mutation

   Promoter

   Aedes aegypti polyubiquitin and Culex quinquefasc…

   Available since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p324_DG

   Plasmid

   #234690

   Purpose

   Golden gate domesticated pET28b with TEV-His6

   Depositor

   Matthew Wohlever

   Article

   Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

   Type

   Empty backbone

   Use

   Tags

   TEV-His6

   Expression

   Bacterial

   Mutation

   No

   Promoter

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLVX-TetOne-Puro-mitoLbNOX-FLAG

   Plasmid

   #218667

   Purpose

   pLVX-TetOne-Puro-mitoLbNOX-FLAG

   Depositor

   Valentin Cracan

   Article

   Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

   Insert

   mitoLbNOX
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   FLAG and MTS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. dCas9 fused to nanobody (anti-beta catenin)

   Plasmid

   #186420

   Purpose

   Expression of dCas9 with C-terminal nanobody fusion recognizing beta catenin tag

   Depositor

   Stephen Elledge

   Article

   Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

   Insert

   dCas9-nanobody fusion (anti-beta catenin)
   

   Use

   CRISPR

   Tags

   6His

   Expression

   Mutation

   Promoter

   Available since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MSCV-N-Flag-HA-IRES-PURO

   Plasmid

   #41033

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Type

   Empty backbone

   Use

   Retroviral; Gateway destination vector

   Tags

   Flag, HA, and IRES Puro

   Expression

   Mammalian

   Mutation

   Promoter

   LTR-driven expression

   Available since

   Oct. 26, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pT3-Neo-EF1a-GFP

    Plasmid

    #69134

    Purpose

    Sleeping Beaquty (SB) vector encoding G418 resistance and GFP from two separate promoters

    Depositor

    Martin Bonamino

    Article

    Chicaybam et al Front Bioeng Biotechnol. 2017 Jan 23;4:9

    Type

    Empty backbone

    Use

    Sleeping beauty transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U3MSCV

    Available since

    Jan. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBRPB CAG-mCherry-IP

    Plasmid

    #106333

    Purpose

    PiggyBac transposon, mCherry-IRES-puro labeling under the control of CAG promoter. Used in chimera experiment of ES cells.

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Insert

    mCherry-IRES-Puro
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUCsRNAP

    Plasmid

    #182746

    Purpose

    Swapping in small RNA promoter, 23-bp spacer sequence, & 19-bp repeats into pJC005 plasmids

    Depositor

    James Collins

    Article

    Chua et al Microbiol Spectr. 2022 Feb 2:e0242721. d

    Insert

    small RNA promoter, a 23-bp spacer sequence, & 19-bp repeats
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-lyso-pHoenix

    Plasmid

    #70112

    Purpose

    Expression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomes

    Depositor

    Christian Rosenmund

    Article

    Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

    Insert

    lyso-pHoenix (Cd63 Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Rat, Mouse)
    

    Use

    Tags

    Arch3, H+/K+ ATPase beta-subunit, HA-Tag, mKate2,…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV9-null

    Plasmid

    #200182

    Purpose

    Expresses AAV2 Rep and AAV9 Cap with nonsense mutation at VR8 site

    Depositor

    Aravind Asokan

    Article

    Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

    Insert

    AAV9 Cap w/ nonsense mutation at VR8
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Replaced amino acids 586-592 in Cap with nonsense…

    Promoter

    Available since

    Oct. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEF-BFP

    Plasmid

    #138272

    Purpose

    Expression of engineered BFP variant that undergoes conversion to GFP after targeted modification with a cytidine deaminase-based DNA base editor

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

    Insert

    BFP H66 PAM
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    H66, PAM motif

    Promoter

    Ef1a

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EGFP-Ki67-mCherry

    Plasmid

    #183748

    Purpose

    Expresses EGFP-Ki67-mCherry

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    MKI67 (MKI67 Human)
    

    Use

    Tags

    Ki-67 with N-terminal EGFP tag and Ki-67 with C-…

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRetroQ-Cre-ERT2

    Plasmid

    #59701

    Purpose

    to make Cre-ERT2 expressing stable cell line by retrovirus

    Depositor

    Richard Youle

    Insert

    Cre-ERT2
    

    Use

    Retroviral

    Tags

    Estrogen receptor (ERT)

    Expression

    Mammalian

    Mutation

    Promoter

    cmvIE

    Available since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LacI-pLacI-pIV pm

    Plasmid

    #218101

    Purpose

    Phagemid that expresses LacI, and pIV under the Lac promoter

    Depositor

    Kevin Esvelt

    Insert

    pIV
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac promoter

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pssAAV-2-hGFAP-GeNL-WPRE-bGHp(A)

    Plasmid

    #221455

    Purpose

    AAV vector expressing GFAP-GeNL

    Depositor

    Maiken Nedergaard

    Article

    Beinlich et al Science. 2024 Mar 29;383(6690):1471-1478

    Insert

    Green enhanced Nano-lantern
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hGFAP

    Available since

    Aug. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CAG-GFP

    Plasmid

    #16664

    Depositor

    Fred Gage

    Article

    Zhao et al J Neurosci. 2006 Jan 4. 26(1):3-11.

    Insert

    eGFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. [pSK581] pRK5 HA-SLC38A9N

    Plasmid

    #136141

    Purpose

    mammalian expression of SLC38A9 N term fragment (1-119)

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

    Insert

    SLC38A9 N term fragment (1-119) (SLC38A9 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET21a-ValRS-His

    Plasmid

    #124122

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Valyl-tRNA ligase (valS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFGC5941DR5-PacI-ath-STTM164

    Plasmid

    #84090

    Purpose

    target miRNA164 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM164
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCherry.90 beta

    Plasmid

    #108223

    Purpose

    Mammalian expression vector for mCherry fusion to human Hsp90 beta

    Depositor

    Didier Picard

    Article

    Picard et al Exp Cell Res. 2006 Nov 15. 312(19):3949-

    Insert

    mCherry-Hsp90b (HSP90AB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET16b-PCNA

    Plasmid

    #134898

    Purpose

    Bacterial expression and purification of human PCNA

    Depositor

    Andrew Deans

    Article

    Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

    Insert

    PCNA (PCNA Human)
    

    Use

    Tags

    untagged

    Expression

    Bacterial

    Mutation

    fully synthetic, codon optimized for bacterial ex…

    Promoter

    T7

    Available since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFA6a-link-ymNeongreen-SpHis5

    Plasmid

    #125704

    Purpose

    Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with SpHIS5 selection

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymNeonGreen
    

    Use

    Tags

    ymNeonGreen

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. DT480

    Plasmid

    #80413

    Purpose

    Expresses 480 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

    Depositor

    Thomas Cooper

    Article

    Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

    Insert

    human DMPK exons 11-15 with 480 interrupted CTG repeats (DMPK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. VEGFR2-mCh

    Plasmid

    #108854

    Purpose

    Encodes for human VEGFR2 fluorescently labeled with mCherry on the C-Terminus via a 3 amino acid (GGS) flexible linker

    Depositor

    Kalina Hristova

    Article

    Sarabipour et al Elife. 2016 Apr 7;5:e13876. doi: 10.7554

    Insert

    KDR (KDR Human)
    

    Use

    Tags

    Labeled with mCherry on the C-Terminus via a 3 am…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLenti-Cas9-P2A-Puro

    Plasmid

    #110837

    Purpose

    Lentiviral vector for expression of Cas9 in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CAG-mCherry-sesRNA-GFP-WPRE-pA

    Plasmid

    #192065

    Purpose

    Expression of mCherry, sesRNA in mammalian cells, with GFP as efRNA.

    Depositor

    Josh Huang

    Article

    Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

    Insert

    sesRNA for Cheta
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDisplay-GRAB-HaloDA1.0-IRES-EGFP-CAAX

    Plasmid

    #234410

    Purpose

    Expresses the chemigenetic dopamine (DA) sensor GRAB_HaloDA1.0 and a membrane-localized EGFP in mammalian cells

    Depositor

    Yulong Li

    Article

    Zheng et al Science. 2025 Jun 5;388: 6751. doi: http

    Insert

    GPCR activation based chemigenetic dopamine (DA) sensor GRAB_HaloDA1.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDisplay-GRAB-HaloDAmut-IRES-EGFP-CAAX

    Plasmid

    #234411

    Purpose

    Expresses the chemigenetic dopamine (DA) control sensor GRAB_HaloDAmut and a membrane-localized EGFP in mammalian cells

    Depositor

    Yulong Li

    Article

    Zheng et al Science. 2025 Jun 5;388: 6751. doi: http

    Insert

    GPCR activation based chemigenetic dopamine (DA) control sensor GRAB_HaloDAmut
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3 HA E2F2

    Plasmid

    #236432

    Purpose

    transient overexpression of E2F2 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

    Insert

    E2F2 (E2F2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3 HA E2F3A

    Plasmid

    #236437

    Purpose

    transient overexpression of E2F3A in mammalian cells

    Depositor

    Michele Pagano

    Article

    Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

    Insert

    E2F3A (E2F3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3 2xFLAG-2xSTREP TFDP2

    Plasmid

    #236445

    Purpose

    transient overexpression of TFDP2 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

    Insert

    TFDP2 (TFDP2 Human)
    

    Use

    Tags

    FLAG-FLAG-STREP-STREP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3 2xFLAG-2xSTREP TFDP1

    Plasmid

    #236444

    Purpose

    transient overexpression of TFDP1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

    Insert

    TFDP1 (TFDP1 Human)
    

    Use

    Tags

    FLAG-FLAG-STREP-STREP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3 2xFLAG-2xSTREP E2F6

    Plasmid

    #236441

    Purpose

    transient overexpression of E2F6 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

    Insert

    E2F6 (E2F6 Human)
    

    Use

    Tags

    FLAG-FLAG-STREP-STREP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3 2xFLAG-2xSTREP E2F4

    Plasmid

    #236439

    Purpose

    transient overexpression of E2F4 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

    Insert

    E2F4 (E2F4 Human)
    

    Use

    Tags

    FLAG-FLAG-STREP-STREP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3 2xFLAG-2xSTREP E2F5

    Plasmid

    #236440

    Purpose

    transient overexpression of E2F5 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

    Insert

    E2F5 (E2F5 Human)
    

    Use

    Tags

    FLAG-FLAG-STREP-STREP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. EW410 IKZF1tar x8-H2B-GFP

    Plasmid

    #236156

    Purpose

    Fuorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with eight IKZF1 binding sites upstream

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    E1b minimal promoter with eight IKZF1 binding sit…

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. CMVTO-hIL2

    Plasmid

    #233387

    Purpose

    secreted human IL-2

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    IL-2 (IL2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCARGO-RMCE-Xist

    Plasmid

    #233268

    Purpose

    CARGO plasmid containing ~22kb dox-inducible Xist transgene

    Depositor

    Mauro Calabrese

    Article

    Trotman et al Mol Cell. 2025 Mar 20;85(6):1117-1133.e1

    Insert

    22kb Xist gene, including introns
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. AiP12316 - pAAV-AiE0467m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2316)

    Plasmid

    #230109

    Purpose

    AiE0467m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    Boaz Levi, The Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pLV-hUV6-sgRNA-dCas9-KRAB-TagBFP2 (identifier AAAA-0244)

    Plasmid

    #202553

    Purpose

    Nontargeting control vector with TagBFP2

    Depositor

    Frederic Meunier

    Article

    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

    Insert

    Humanized dCas9-KRAB-TagBFP2 (tag blue fluorescent protein 2) (TRIM28 S. Pyogenes (dCas9), H. sapiens (KRAB), Entacmaea quadricolor (TagBFP2))
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

    Expression

    Mutation

    Puromycin-resistance gene in the pLV hU6-sgRNA hU…

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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45. pSEVA63_HelperΔgIII

    Plasmid

    #190433

    Purpose

    M13 helper genes cloned into the pSEVA631 vector, except the minor coat protein coding gene gIII (also called gp3, or g3p). This plasmid does not carry an M13 packaging signal.

    Depositor

    Manish Kushwaha

    Article

    Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

    Insert

    M13 genes I-V and VII-XI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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46. pEF2-FL-hMer_gR1

    Plasmid

    #225993

    Purpose

    Expresses a chimeric contruct having N-terminal human MerTK and C-terminal human Interferon Gamma Receptor

    Depositor

    Sergei Kotenko, Raymond Birge

    Article

    Tsou et al J Biol Chem. 2014 Sep 12;289(37):25750-6

    Insert

    human MerTK N-Terminal human Interferon Gamma R1 Receptor C-Terminal fusion (MERTK, IFNGR1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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47. pET28b-ptetO::bac::gfp

    Plasmid

    #217882

    Purpose

    expresses the bac-BGC in bacterial cells, produces bacillamide D

    Depositor

    Tobias A. M. Gulder

    Article

    Hohmann et al Commun Biol. 2024 May 28;7(1):655. doi:

    Inserts

    Dehydrogenase
    Non-ribosomal peptide synthetase
    Aminotransferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ptetO

    Available since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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48. pLV_Tre3g_CalcireticulinSS_mTurquoise_D4-cameleon_REAch2_KDEL

    Plasmid

    #216253

    Purpose

    FRET-based calcium sensor located in the ER lumen, Tet-inducible

    Depositor

    Edward Avezov

    Article

    Crapart et al Proc Natl Acad Sci U S A. 2024 Mar 26;12

    Insert

    ER-targeted D4 second generation cameleon (FRET calcium sensor)
    

    Use

    Lentiviral

    Tags

    REAch and mTurquoise

    Expression

    Mutation

    Promoter

    TRE3G

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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49. pRDB_118

    Plasmid

    #216089

    Purpose

    Cas9 [Sp] CRISPRa targeting CD47, positive control

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    CD47 guide
    

    Use

    CRISPR and Lentiviral; Positive controls

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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50. pT2/shCol1a1/GFP4_Seq1.3

    Plasmid

    #206136

    Purpose

    Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

    Insert

    COL1A1 (Col1a1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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