We narrowed to 5,621 results for: pcas

1. pCAG-hHDAC6

   Plasmid

   #21099

   Depositor

   Connie Cepko

   Article

   Chen et al Science. 2009 Jan 9. 323(5911):256-9.

   Insert

   Histone Deacetylase 6 (HDAC6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 5, 2009

   Availability

   Academic Institutions and Nonprofits only

2. pCAG-C-CreintG

   Plasmid

   #69573

   Purpose

   One half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for expression in mammalian cells

   Depositor

   Connie Cepko

   Article

   Tang et al Nat Neurosci. 2015 Sep;18(9):1334-41. do

   Insert

   kozak consensus - C-CreintG
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMA-SpCas9-g7

   Plasmid

   #80790

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 7/17/27 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSL2621 (pCas8_Vch)

   Plasmid

   #200893

   Purpose

   Expresses human codon-optimized V. cholerae Cas8 from a CMV promoter.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   VchCas8
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSL2623 (pCas6_Vch)

   Plasmid

   #200895

   Purpose

   Expresses human codon-optimized V. cholerae Cas6 from a CMV promoter.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   VchCas6
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-p21_WT

   Plasmid

   #40202

   Depositor

   Philipp Mergenthaler

   Article

   Mergenthaler et al J Cereb Blood Flow Metab. 2013 Mar;33(3)

   Insert

   p21 (CDKN1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CAG

   Available since

   March 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAX APLP1

   Plasmid

   #30141

   Depositor

   Dennis Selkoe, Tracy Young-Pearse

   Article

   Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

   Insert

   Amyloid Precursor Like Protein 1 (APLP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pCAG-Chimeric_Butterfly_CY_1.0

   Plasmid

   #59800

   Purpose

   Genetically encoded fluorescent voltage indicator

   Depositor

   Thomas Knopfel

   Article

   Mishina et al Front Mol Neurosci. 2014 Sep 29;7:78. do

   Insert

   Cyan-Yellow Chimeric Butterfly Voltage Sensitive Fluorescent Protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCas_5+7_gfp

   Plasmid

   #60595

   Purpose

   encodes a reversed Int7 gene flanked by the attB/P sites of Int5, as well as a reversed GFP (GFPmut33) gene flanked by the attB/P sites of Int7.

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Cas_5+7_gfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-Bmp7

    Plasmid

    #163591

    Purpose

    Expresses BMP-7 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmp7 (Bmp7 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCaRNA3-eIFiso4E120(+)

    Plasmid

    #86092

    Purpose

    This vector is used to specifically silence the eIF(iso)4E gene in peach via VIGS.

    Depositor

    Aiming Wang

    Article

    Cui et al Plant Biotechnol J. 2016 Aug 27. doi: 10

    Insert

    eIFiso4E
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-Bmp2

    Plasmid

    #163587

    Purpose

    Expresses BMP-2 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmp2 (Bmp2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. LLP787_SnoopCatcher-EZH2-FL-CO

    Plasmid

    #211780

    Purpose

    SnoopTag counterpart binding domain, SnoopCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    aGCN4-DNMT3A
    

    Use

    Tags

    3xTy1

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect DNMT3A…

    Promoter

    pEF1a and pSV40

    Available since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-mRhubarb720

    Plasmid

    #197212

    Purpose

    Expresses the protein of mRhubarb720 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    mRhubarb720
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-HASAP1-ST

    Plasmid

    #138965

    Purpose

    chemigenetic voltage indicator, soma targeted

    Depositor

    Eric Schreiter

    Article

    Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

    Insert

    HASAP1-ST
    

    Use

    Tags

    somatic localization tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-mTcte1-PA

    Plasmid

    #176469

    Purpose

    Expression vector of mouse t-complex-associated testis expressed 1 (Tcte1) tagged with PA at C-terminus.

    Depositor

    Masahito Ikawa

    Article

    Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

    Insert

    t-complex-associated testis expressed 1 (Tcte1 Mouse)
    

    Use

    Tags

    PA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. eSpCas9-plus

    Plasmid

    #126767

    Purpose

    Expression plasmid for human codon-optimized increased fidelity eSpCas9-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    eSpCas9-plus
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    K848A, R1060A; amino acids 1005-1013 replaced wit…

    Promoter

    CBh

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGIG_V5-PPP6C

    Plasmid

    #92013

    Purpose

    Expresses V5-PPP6C, includes IRES-Emerald

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    PPP6C (PPP6C Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-VHC

    Plasmid

    #112618

    Purpose

    expression plasmid with CAG promoter driving multicistronic cassette H2BVenus_p2A_HygroR_p2A_CreERt2

    Depositor

    Stefan Heller

    Article

    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

    Inserts

    H2B-Venus (H2BC3 Human, Aequorea victoria)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCas9-CAT

    Plasmid

    #80323

    Purpose

    Encodes Cas9 and chloramphenicol acetyltransferase (CAT)

    Depositor

    Sebastian Lourido

    Article

    Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

    Inserts

    Cas9
    Chloramphenicol acetyltransferase
    

    Use

    CRISPR

    Tags

    3X FLAG and NLS

    Expression

    Mutation

    Promoter

    TgTUB1

    Available since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCAGGS-ROSAf

    Plasmid

    #54323

    Purpose

    TALEN expression construct for targeting the murine ROSA26 locus. It recognize the sequence: CTGCAACTCCAGTCT. Can be used with the pBS SK mCherryROSAbsr plasmid

    Depositor

    Silvestro Conticello

    Insert

    TALEN for the murine ROSA26 locus
    

    Use

    Mouse Targeting and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGG

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCAG-EnvARGCD

    Plasmid

    #100800

    Purpose

    express fusion protein of EnvA (ASLV-A envelope protein) extracellular and transmembrane domains with rabies virus SAD B19 strain glycoprotein intracellular domain

    Depositor

    Ian Wickersham

    Insert

    fusion protein of EnvA extracellular and transmembrane domain with rabies virus glycoprotein intracellular domain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSL2622 (pCas7_Vch)

    Plasmid

    #200894

    Purpose

    Expresses human codon-optimized V. cholerae Cas7 from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    VchCas7
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCAGGS-ROSAr

    Plasmid

    #54324

    Purpose

    TALEN expression construct for targeting the murine ROSA26 locus. It recognize the sequence: GGGAGTCTTCTGGGC. Can be used with the pBS SK mCherryROSAbsr plasmid

    Depositor

    Silvestro Conticello

    Insert

    TALEN for the murine ROSA26 locus
    

    Use

    Mouse Targeting and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGG

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCAG-tdTHC

    Plasmid

    #112621

    Purpose

    expression plasmid with CAG promoter driving multicistronic cassette H2BtdTomato_p2A_HygroR_p2A_CreERt2

    Depositor

    Stefan Heller

    Article

    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

    Inserts

    H2B-tdTomato (H2BC3 Human, Anthozoa)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    

    Use

    Cre/Lox

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCAGGS-AcGFP-C

    Plasmid

    #203325

    Purpose

    Express N-terminal AcGFP-fused protein in mammalian cell

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCAGGS-6020nes

    Plasmid

    #108656

    Purpose

    A JNK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

    Depositor

    Michiyuki Matsuda

    Article

    Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

    Insert

    hyBRET-JNK-EV
    

    Use

    Tags

    XFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGGB-SpCas9

    Plasmid

    #210533

    Purpose

    Cloning A.thaliana codon optimized SpCas9 CDS as GreenGate module B

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    SpCas9
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAG-B19M

    Plasmid

    #59923

    Purpose

    Expression vector for Rabies virus SAD B19 matrix protein gene

    Depositor

    Ian Wickersham

    Insert

    Rabies virus matrix protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCas9-VRER_2A_GFP

    Plasmid

    #75475

    Purpose

    Cas9 VRER variant that detects NGCG PAM, combined with 2A_GFP for expression control

    Depositor

    Marc Tessier-Lavigne

    Article

    Paquet et al Nature. 2016 May 5;533(7601):125-9. doi:

    Insert

    Cas9-VRER
    

    Use

    CRISPR

    Tags

    2A_GFP

    Expression

    Mammalian

    Mutation

    D1135V, G1218R, R1335E, T1337R

    Promoter

    CAG

    Available since

    May 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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