We narrowed to 10,863 results for: HA
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Plasmid#215914PurposeExpression of full-length TNRC6A with S1616D/S1691D mutationDepositorInsertTNRC6A (TNRC6A Human)
UseTagsFlag-HA-SBPExpressionMammalianMutationFull length of TNRC6A, S1616D, S1691DPromoterAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA.5.1-FHS-TNRC6A-FL-S1616A/S1691A
Plasmid#215915PurposeExpression of full-length TNRC6A with S1616A/S1691A mutationDepositorInsertTNRC6A (TNRC6A Human)
UseTagsFlag-HA-SBPExpressionMammalianMutationFull length of TNRC6A, S1616A, S1691APromoterAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA.5.1-FHS-TNRC6A-SD-S1332D/S1346D
Plasmid#215906PurposeExpression of TNRC6A-silencing domain (SD) with S1332D/S1346D mutationDepositorInsertTNRC6A (TNRC6A Human)
UseTagsFlag-HA-SBPExpressionMammalianMutationcontain aa1224-1709, S1332D, S1346DPromoterAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA.5.1-FHS-TNRC6A-SD-S1332A/S1346A
Plasmid#215907PurposeExpression of TNRC6A-silencing domain (SD) with S1332A/S1346A mutationDepositorInsertTNRC6A (TNRC6A Human)
UseTagsFlag-HA-SBPExpressionMammalianMutationcontain aa1224-1709, S1332A, S1346APromoterAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA.5.1-FHS-TNRC6A-SD-S1616D/S1691D
Plasmid#215908PurposeExpression of TNRC6A-silencing domain (SD) with S1616D/S1691D mutationDepositorInsertTNRC6A (TNRC6A Human)
UseTagsFlag-HA-SBPExpressionMammalianMutationcontain aa1224-1709, S1616D, S1691DPromoterAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_dCas9-XTEN-KRAB-p2A-mCherry
Plasmid#222107PurposeDonor vector for CRISPRi integration at AAVS1 with an mCherry fluorophoreDepositorInsertCRISPRi-kox1(KRAB) (cas9 S. pyogenes, Human)
UseAAV and CRISPRTagsHA and P2A-mCherryExpressionMammalianMutationD10A, H840APromoterAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
Stargazin-EBFP2-FRB
Plasmid#222417PurposeTo express a plasma membrane "anchor" for rapamycin-induced heterodimerisation in mammalian cellsDepositorAvailable SinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
dCas9-FCPF
Plasmid#220131PurposeExpresses dCas9-FCPF in mammalian cellsDepositorInsertdCas9-FCPF (cas9 S. pyogenes, Synthetic)
UseTagsFlag and HAExpressionMammalianMutationPromoterCMVAvailable SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_dCas9-XTEN-KRAB-p2A-GFP
Plasmid#222108PurposeDonor vector for CRISPRi integration at AAVS1 with a GFP fluorophoreDepositorInsertCRISPRi-kox1(KRAB) (cas9 S. pyogenes, Human)
UseAAV and CRISPRTagsHA and P2A-GFPExpressionMammalianMutationD10A, H840APromoterAvailable SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEM-601[insert(+1) TA-rich]
Plasmid#221196Purpose601 nucleosome positioning sequence with an additional base pair inserted 22 nt from the dyad on the TA-rich side of the 601DepositorInsert601[insert(+1)TA-rich]
UseUnspecifiedTagsExpressionMutation601 has an additional mutation added 22 bp from d…PromoterAvailable SinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
GfaABC1D-Crym-BioID2-BioID2
Plasmid#200070PurposeTo study u-crystallin protein interactorsDepositorInsertGfaABC1D-Crym-BioID2-BioID2
UseAAV and Mouse TargetingTagsHA-tagExpressionMutationPromoterGfaABC1DAvailable SinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNS49 (enAsCas12a-KRAB piggyBac)
Plasmid#217335PurposepiggyBac expresion of enAsCas12a-KRABDepositorInsertenAsCas12a
UseCRISPRTags6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2ExpressionMammalianMutationE174R/S542R/K548RPromoterCAGAvailable SinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDY279
Plasmid#182959PurposeStr resistant,CoE1* ori. CRISPR/Cas9 gRNA under constitutive J23119 promoter. gRNA targeting E. coli ptsI codon 2~9. HRT has synonymous mutations in ptsI. (for 1st round editing)DepositorInsertgRNA (ttcaggcattttagcatccc), homologous repair template for ptsI
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDY281
Plasmid#182960PurposeAmp resistant, CoE1* ori. CRISPR/Cas9 induced by AHTc, gRNA under constitutive J23119 promoter. gRNA targeting E. coli ptsI codon 2~9. HRT has synonymous mutations of ptsI. (for 2nd round editing)DepositorInsertgRNA (acctggtgaagccaatattc), homologous repair template for ptsI
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
FKBP12-CB2mutx2-mTagBFP2-HAx2
Plasmid#207147PurposeTandem mutant calcium-buffer with rapalog dimerizerDepositorInsertCalbindin-2 (CALB2 Human)
UseTagsFKBP12, HA, and mTagBFP2ExpressionMammalianMutationE40Q, E87Q, E131Q, E175Q, E219QPromoterCMVAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
185_pETcon_SARS2_BQ1-1
Plasmid#208085Purposeyeast surface display of the SARS-CoV-2 BQ.1.1 variant RBDDepositorInsertSARS-CoV-2 BQ.1.1 RBD (S SARS-CoV-2 virus, Budding Yeast)
UseTagsHA and c-MycExpressionYeastMutationPromoterAvailable SinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
186_pETcon_SARS2_XBB1-5
Plasmid#208086Purposeyeast surface display of the SARS-CoV-2 XBB.1.5 variant RBDDepositorInsertSARS-CoV-2 XBB.1.5 RBD (S SARS-CoV-2 virus, Budding Yeast)
UseTagsHA and c-MycExpressionYeastMutationPromoterAvailable SinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcS-RDG-C1C2
Plasmid#200163PurposeThis plasmid encodes non-binder control monobody (RDG) fused to the extracellular vesicle binding domain (C1C2) of lactadherin for surface engineering extracellular vesicles.DepositorInsertsRDG-C1C2
C1C2 domain of lactadherin
UseTagsHA and HISExpressionMammalianMutationsequence adopted from doi: 10.1371/journal.pone.0…PromoterAvailable SinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CALPAIN-7-mCherry, F98D
Plasmid#180653PurposeLentiviral expression vector for CALPAIN-7. Used for generating cell lines. F98D mutation. Has C-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-53.DepositorInsertCALPAIN-7
UseLentiviralTagsmCherryExpressionMammalianMutationsiRNA resistant to: GCACCCAUACCUUUACAUUPromoterAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 RPS6KL1 MIT, residues 20-140
Plasmid#180623PurposeBacterial expression for RPS6KL1 MIT domain, residues 20-140. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-32.DepositorInsertRPS6KL1 (RPS6KL1 Human)
UseTagsHIS-SUMOExpressionBacterialMutationResidues 20-140PromoterT7Available SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-THTAP-SNX15, residues 263-342
Plasmid#180624PurposeBacterial expression for SNX15 MIT residues 263-342. Has N-terminal HIS-GST tag. Internal ID:WISP21-130.DepositorAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 SNX15 MIT residues 263-342
Plasmid#180625PurposeBacterial expression for SNX15 MIT residues 263-342. Has N-terminal HIS-SUMO tag. Internal ID:WISP21-14DepositorInsertSNX15 (SNX15 Human)
UseTagsHIS-SUMOExpressionBacterialMutationResidues 263-342PromoterT7Available SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 USP8, residues 1-147
Plasmid#180636PurposeBacterial expression for USP8 MIT domain, residues 1-147. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-86.DepositorAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET16B VPS4A, residues 1-84
Plasmid#180638PurposeBacterial expression for VPS4A MIT domain residues 1-84. Has N-terminal HIS-TAG. Internal ID: WISP05-43DepositorAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CALPAIN-7 residues 1-165
Plasmid#180611PurposeBacterial expression vector for CALPAIN-7 residues 1-165, tandem MIT domains. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-25DepositorInsertCALPAIN-7
UseTagsHIS-SUMOExpressionBacterialMutationResidues 1-165PromoterT7Available SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUBQ10:LLFLS2-2
Plasmid#202188PurposeExpress 'Frost Lisbon' Lemon FLS2-2 gene under the UBQ10 promoterDepositorInsertFlagellin sensing 2
UseTagsHAExpressionPlantMutationPromoterAtUBQ10Available SinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUBQ10:WNFLS2-2
Plasmid#202190PurposeExpress 'Washington navel' Orange FLS2-2 gene under the UBQ10 promoterDepositorInsertFlagellin sensing 2
UseTagsHAExpressionPlantMutationPromoterAtUBQ10Available SinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUBQ10:WNFLS2-1
Plasmid#202189PurposeExpress 'Washington navel' Orange FLS2-1 gene under the UBQ10 promoterDepositorInsertFlagellin sensing 2
UseTagsHAExpressionPlantMutationPromoterAtUBQ10Available SinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUBQ10:LLFLS2-1
Plasmid#202187PurposeExpress 'Frost Lisbon' Lemon FLS2-1 gene under the UBQ10 promoterDepositorInsertFlagellin sensing 2
UseTagsHAExpressionPlantMutationPromoterAtUBQ10Available SinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2/sh-FYN1-GFP_Seq_C
Plasmid#201399PurposeKnockdown of FYN tyrosine kinase. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertFYN
UseTagsExpressionMammalianMutationPromoterAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACT2-μ1B (137-423)
Plasmid#198172PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-1 μ1B (137-423) fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-1 μ1B (137-423)
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationPromoterADH1Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
mt-DdCBE G1397-N
Plasmid#174562Purposemitochondrial-targeting DdCBE (split DddA, G1397-N) Golden Gate destination vectorDepositorInsertDdCBE G1397-N
UseTagsHAExpressionPlantMutationPromoterPcUbiAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
cp-DdCBE G1397-N
Plasmid#174558PurposeChloroplast-targeting DdCBE (split DddA, G1397-N) Golden Gate destination vectorDepositorInsertDdCBE G1397-N
UseTagsHAExpressionPlantMutationPromoterPcUbiAvailable SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pClgn1.1_Kctd19-3xHA
Plasmid#173497PurposeExpress Kctd19-3xHA under the mouse Clgn promoter.DepositorInsertPotassium Channel Tetramerization Domain Containing 19 (Kctd19 Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable SinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPRIChp-hOtub1
Plasmid#174743PurposeGene overexpressionDepositorAvailable SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJS697
Plasmid#168778PurposeYeast surface display plasmid backbone that can be combined with pJS699 through homologous recombinationDepositorTypeEmpty backboneUseTagsCmyc and HAExpressionYeastMutationPromoterAvailable SinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-deltaA1-R1597W-VWF
Plasmid#124826PurposeExpresses multimeric human VWF protein without A1 domain and a R1597W mutation in A2 domain. This protein has C-terminal c-Myc tag.DepositorInsertVWF without A1 domain and R1597W mutation A2 domain (VWF Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable SinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-deltaA1-D1498A-VWF
Plasmid#124825PurposeExpresses multimeric human VWF protein without A1 domain and a D1498A mutation in A2 domain. This protein has C-terminal c-Myc tag.DepositorInsertVWF without A1 domain and D1498A mutation A2 domain (VWF Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable SinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUCHR-CD5HA2-P2A-smAID-mClover
Plasmid#110658PurposeLentiviral vector expressing cell surface HA-tag and monomeric GFP N-terminaly fused with auxin inducible degronDepositorInsertCD5HA2-P2A-AID-mClover
UseLentiviralTagsExpressionMutationPromoterCMVAvailable SinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-Ost1-pro-alphaf-Btl2
Plasmid#117665PurposeStudy secretion efficiency of Btl2DepositorInsertpre-Ost1-pro-alphaf-Btl2
UseTagsExpressionYeastMutationThis plasmid encodes the lipase Btl2 together wit…PromoterpAOX1Available SinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only