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19,210 results

Showing: 11201 - 11250 of 19210 results

  2. pICE-FLAG-NAT10-siR-G641E

    Plasmid
    #59366
    Purpose
    Plasmid for constitutive or doxycycline-inducible expression of G641E mutant of human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Steve Jackson
    Article
    Larrieu et al Science. 2014 May 2;344(6183):527-32. do
    Insert
    NAT10 NM_024662 (NAT10 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    G641E and silent mutations to render the cDNA res…
    Promoter
    CMV-tet
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    59366-plasmid-map-sequence-id-92067

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  6. pET21b-Casp1-His

    Plasmid
    #11809
    Depositor
    Guy Salvesen
    Insert
    Caspase-1 (CASP1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 12, 2006
    Availability
    Academic Institutions and Nonprofits only
    11809-plasmid-map-sequence-id-205971

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  13. pAAV-HyPer-DAAO-NES

    Plasmid
    #119164
    Purpose
    Fusion of hydrogen peroxide fluorescent biosensor HyPer and D-amino acid oxidase; excluded from nucleus. Allows H2O2 production/detection by adding D-amino acids. AAV expression vector; CMV promoter.
    Depositor
    Thomas Michel
    Article
    Steinhorn et al Nat Commun. 2018 Oct 2;9(1):4044. doi: 1
    Insert
    HyPer-DAAO-NES
    Use
    AAV
    Tags
    NES
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    119164-plasmid-map-sequence-id-232464

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  14. pScarlessHD-3xFLAG-DsRed

    Plasmid
    #80820
    Purpose
    Vector containing a cassette for scarless insertion of 3xFLAG and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.
    Depositor
    Kate O'Connor-Giles
    Insert
    3xFLAG-piggyBac-DsRed
    Use
    Unspecified
    Tags
    3xFLAG
    Expression
    Mutation
    Promoter
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    80820-plasmid-map-sequence-id-152798

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  21. CHRM3-Tango

    Plasmid
    #66250
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    CHRM3 (CHRM3 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  23. pET_RTX(exo-)-6xHis

    Plasmid
    #145028
    Purpose
    pET vector for IPTG-inducible, T7-based expression of the exonuclease deficient version of RTX bearing a C-terminal 6xHis tag
    Depositor
    Andrew Ellington
    Article
    Bhadra et al BioProtocol 2021
    Insert
    RTX(exo-)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    N210D
    Promoter
    T7
    Available since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    145028-plasmid-map-sequence-id-292638

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  24. pLL3.7m-psMEK1tight

    Plasmid
    #89362
    Purpose
    A lenti-viral vector expressing photoswitchable MEK1 with minimum basal activity in mammalian cells
    Depositor
    Michael Lin
    Article
    Zhou et al Science. 2017 Feb 24;355(6327):836-842.
    Insert
    psMEK1tight (MAP2K1 Synthetic, Human)
    Use
    Lentiviral
    Tags
    Human influenza hemagglutinin (HA) tag and pdDron…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    89362-plasmid-map-sequence-id-176059

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  29. pGFP FRNK

    Plasmid
    #50518
    Purpose
    This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.
    Depositor
    Kenneth Yamada
    Article
    Gu et al J Cell Biol. 1999 Jul 26;146(2):389-403.
    Insert
    protein tyrosine kinase, mutated
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    truncated (NT2186-3268)
    Promoter
    CMV
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  38. pIA33

    Plasmid
    #120803
    Purpose
    E. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfp
    Depositor
    Craig Ellermeier, David S. Weiss
    Article
    Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-
    Inserts
    deactivated nuclease dCas9, codon-optimized for C. difficile
    single guide RNA targeting red fluorescent protein
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Base-pairing region targets red fluorescent prote…
    Promoter
    Pgdh and Pxyl
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120803-plasmid-map-sequence-id-235316

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  41. pLIX-REST

    Plasmid
    #91896
    Purpose
    Doxycycline inducible expression of mouse REST
    Depositor
    Julien Sage
    Insert
    REST (Rest Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE promoter, Tet ON
    Available since
    June 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    91896-plasmid-map-sequence-id-180652

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  45. COVID-SARS2 NSP14/10

    Plasmid
    #159613
    Purpose
    Bacterial co-expression vector fo Covid-SARS2 NSP14 and NSP10
    Depositor
    Opher Gileadi
    Article
    Baddock et al Nucleic Acids Res. 2022 Jan 17. pii: 650
    Inserts
    Covid-SARS2 Nsp14 (ORF1ab Synthetic, COVID-SARS2)
    Covid-SARS2 Nsp10
    Use
    Tags
    His6, TEV cleavage site and none
    Expression
    Bacterial
    Mutation
    Codon-optimized for E. coli expression
    Promoter
    (Bicistronic) and T7 - LacO
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    159613-plasmid-map-sequence-id-311056

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  48. SHMT2

    Plasmid
    #25479
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SHMT2 (SHMT2 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    A269T
    Promoter
    Available since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    25479-plasmid-map-sequence-id-168679

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Showing: 11201 - 11250 of 19210 results