We narrowed to 14,204 results for: RING;
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Plasmid#41667DepositorInsertMus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
TagsYFPExpressionMammalianMutation“Monomerizing” YFP A206K mutation; 3 C-terminal-m…PromoterCMVAvailable SinceJan. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AviMta2-3xFiBsd
Plasmid#140964Purposefull-length wt Avi-Mta2-3XFLAG-iBsd cDNA w/CAG promoter and Blasticidin resistance, cloned from mouse ES cellsDepositorInsertmetastasis-associated gene family, member 2 (Mta2 Mouse)
Tags3xFLAG, Avi, and TEVExpressionMammalianPromoterCAGAvailable SinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBabe RFP-DN-FOXO1-HA neo
Plasmid#45813DepositorAvailable SinceJune 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMX-IY HA-GBRPL1
Plasmid#89297PurposeFor expression of the mammalian Atg8 protein GABARAPL1 tagged with HADepositorAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLPC-FLAG.Mm.DMAP1
Plasmid#184650PurposeRetroviral expression of mouse DMAP1DepositorAvailable SinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNMSB99
Plasmid#199308PurposeMosSCI insertion of myo-3p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR at ttTi5605DepositorInsertmyo-3p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR
ExpressionWormMutationmTagBFP2 from pJJR81, ChR2(H134R;D156C), C. elega…Promotermyo-3Available SinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR-E2F1
Plasmid#37141DepositorInsertE2F1 (E2f1 Fly)
UseGatewayAvailable SinceJune 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMX-IY GBRPL1
Plasmid#89294PurposeFor expression of the mammalian Atg8 protein GABARAPL1 without a tagDepositorAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmx-MiGT
Plasmid#172397PurposeRetroviral expression vector for murine direct cardiac reprogrammingDepositorAvailable SinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ289 (ErCas12a)
Plasmid#138126PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized ErCas12a without promoterDepositorInsertErCas12a
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantAvailable SinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
3019_pETcon-SARS-CoV-2-RBD_E484K
Plasmid#184404Purposeyeast surface display of the SARS-CoV-2 Eta variant RBDDepositorInsertSARS-CoV-2 Eta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
TagsHA and c-MycExpressionYeastMutationE484KAvailable SinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO-miR-124-sponge (pos. CTRL)
Plasmid#117327PurposeDual luciferase assay positive control for miR-124 bindingDepositorInsertmiR-124 sponge
UseLuciferaseTagsluciferaseExpressionMammalianPromoterMurine PGK-1Available SinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO-miR-124-sponge_rev(neg. CTRL)
Plasmid#117326PurposeDual luciferase assay negative control for miR-124 bindingDepositorInsertreverse miR-124 sponge
UseLuciferaseTagsluciferaseExpressionMammalianPromoterMurine PGK-1Available SinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-TPD54(R159E)
Plasmid#172453PurposeExpression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal mCherry-FKBP tag R159E mutantDepositorInsertTPD54 (TPD52L2 Human)
TagsmCherry-FKBPExpressionMammalianMutationchanged Arginine 159 to Glutamic AcidPromoterCMVAvailable SinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CreLite
Plasmid#131785PurposeAAV donor/transfer vector expressing CreLite system components, PhyBΔCreC and PIF6CreN, driven by CBh promoterDepositorInsertPhyBCreC-P2A-PIF6CreN
UseAAV, Cre/Lox, and Synthetic BiologyExpressionMammalianPromoterCBhAvailable SinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3-SV40P-Tet2 enhancer H1
Plasmid#63883PurposeLuciferase reporter plasmid used to study transcriptional control of murine Tet2DepositorInsertTet2 enhancer fragment H1
UseLuciferaseExpressionMammalianPromoterSV40Available SinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBabe-PI(WT)-Rac1(WT)-mCherry
Plasmid#87550PurposePI(WT): Photo-inhibitable/blue light sensitive Rac1DepositorAvailable SinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBabe-PI(WT)-Rac1(Q61L)-mCherry
Plasmid#87553PurposePI(WT): Photo-inhibitable/blue light sensitive; const. active Rac1DepositorInsertsTagsmCherryExpressionBacterial and MammalianMutationQ61LPromoterCMVAvailable SinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO GFP MPS1 K-R
Plasmid#59819PurposeAllows the integration of GFP MPS1 K-R in the genome and Tet-inducible expression.DepositorInsertMps1 (TTK Human)
TagsEmGFPExpressionMammalianMutationKD, catalytically inactive, D664APromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable SinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUHD-HA-AML1-ETO
Plasmid#12430DepositorTagsHAExpressionMammalianMutationBstXI-SacII fragment in pUHD-AML1-ETO replaced wi…Available SinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMX-IY LC3A
Plasmid#89290PurposeFor expression of the mammalian Atg8 protein LC3A without a tagDepositorAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
tRNA-gRNA position [D1_n-1] (GB1208)
Plasmid#75409PurposetRNA and scaffold for the assembly of GBoligomers for the first position (positon [D1_n-1]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter (2-part multiplexing)DepositorInserttRNA-gRNA position [D1_n-1]
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
S1 1029 CP ABEMax strategy 1
Plasmid#135357PurposeExpression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the N-terminusDepositorInsertABEMax-nSpyCas9 aa 1029
UseCRISPRExpressionMammalianMutationWTAvailable SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRV-RB
Plasmid#106374Purposecreating stable line with RFP and BSD resistanceDepositorInsertmRFP and blasticidin resistance gene
UseRetroviralExpressionMammalianAvailable SinceFeb. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AviMta3-3xFiBsd
Plasmid#140962Purposefull-length wt Avi-Mta3-3XFLAG-iBsd cDNA w/CAG promoter and Blasticidin resistance, cloned from mouse ES cellsDepositorInsertmetastasis associated 3 (Mta3 Mouse, Synthetic)
Tags3xFLAG, Avi, and TEVExpressionMammalianPromoterCAGAvailable SinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Ctdnep1_D67E-2xFlag
Plasmid#196519PurposeExpression of Ctdnep1_D67E-2xFlagDepositorInsertCtdnep1_D67E-2xFlag (CTDNEP1 Human)
Tags2xFlagExpressionMammalianMutationD67E mutation (phosphastase dead)PromoterCMV promoter; T7 promoterAvailable SinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+CeGFP-Ctdnep1WTsiR
Plasmid#196517PurposeExpression of Ctdnep1WT-GFPDepositorInsertCtdnep1WTsiR (CTDNEP1 Human)
TagseGFPExpressionMammalianMutationSilent mutations for siRNA resistancePromoterCMV promoter; T7 promoterAvailable SinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/BirA/FLAG-DDX54
Plasmid#97063PurposeExpresses BirA/FLAG-tagged DDX54 in mammalian cellsDepositorAvailable SinceJuly 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL3-SV40P-Tet1 enhancer E
Plasmid#63880PurposeLuciferase reporter plasmid used to study transcriptional control of murine Tet1DepositorInsertTet1 enhancer fragment E
UseLuciferaseExpressionMammalianPromoterSV40Available SinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNL2119 pARF7 GG0-GG1
Plasmid#195910PurposeLevel 0 pARF7 promoter with GG0 and GG1 golden gate linkersDepositorInsertpARF7 (NPH4 Mustard Weed)
UseSynthetic BiologyAvailable SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRRL.SIN.EF1A.JAG1-F1.218.CAR-2G
Plasmid#194459PurposeCAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.DepositorInsertAnti-JAG1 CAR
UseLentiviralAvailable SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
tRNA-gRNA position [n] (GB1207)
Plasmid#75408PurposetRNA and scaffold for the assembly of GBoligomers for the last position (positon [n]) of a polycistronic tRNA-gRNA (2- and 3-part multiplexing)DepositorInserttRNA-gRNA position [n]
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCVL.SSA TLR (CCR5 TALEN Sce Spacer)
Plasmid#46941PurposeCodes for the SSA-TLR with the target site for the CCR5 TALEN with the spacer region corresponding to I-Sce I in a lentiviral backboneDepositorInserts5' iRFP arm
eGFP with TALEN TS
+3 mCherry
3' iRFP arm
UseLentiviralExpressionBacterial and MammalianMutationembedded TALEN TS from 163-218, truncated 25 amin…PromoterSFFVAvailable SinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-3-F37A+L196F+V252A+C253G-hADPRM
Plasmid#107163PurposeExpresses specific cyclic-ADP-ribose (cADPR) phosphohydrolase in Escherichia coli as a GST fusion protein that can be cut with PreScission(TM) protease to yield active enzymeDepositorInsertF37A+L196F+V252A+C253G-hADPRM GST-mutant cADPR phosphohydrolase gene
TagsGSTExpressionBacterialPromotertacAvailable SinceMarch 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSL011_AAVS1-puro-9xTetO-pEF-H2B-mCitrine-pRSV-H2B-mCherry
Plasmid#179428PurposeDual fluorescent reporter construct with no spacer, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locusDepositorInsertTRE-cit-(nospacer)-mch
ExpressionMammalianPromoterpEF alpha, pRSVAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
CD8-dCherry-FRB
Plasmid#100739PurposeTo express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis (dark mCherry K70N)DepositorInsertCD8A (CD8A Human)
TagsFRB and dCherryExpressionMammalianMutationDark mCherry (K70N) non-fluorescentPromoterCMVAvailable SinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRV-GN
Plasmid#106373Purposecreating stable line with GFP and G418 resistanceDepositorInserteGFP and geneticin/G418 resistance gene
UseRetroviralExpressionMammalianAvailable SinceFeb. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGB 35s:dCas:EDLL:tNos (GB1190)
Plasmid#75404PurposeTranscriptional unit of (human codon optimized) inactivated Cas9 fused to the EDLL Transcriptional ActivatorDepositorInsertdCas:EDLL
UseCRISPR and Synthetic BiologyExpressionPlantPromoter35SAvailable SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAR69_STRI_m(KS_MAT)_H8_pET22b
Plasmid#122849PurposeExpresses the condensing part of murine type I fatty acid synthase (FASN) consisting of the KS and MAT domain in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.DepositorInsertm(KS_MAT) (Fasn Mouse)
TagsHis8 tag and Twin-Strep tagExpressionBacterialMutationwildtypePromoterT7 promoterAvailable SinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIG-707pUC19-tNGFR-P2A-TTC37
Plasmid#186122PurposeKnockin of truncated NGFR to target geneDepositorAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMX-IY LC3B
Plasmid#89291PurposeFor expression of the mammalian Atg8 protein LC3B without a tagDepositorAvailable SinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTol2-PcyA-IRES-HO1
Plasmid#131787PurposeTol2 bicistronic vector expressing PcyA and HO1 for Phycocyanobilin (PCB) synthesis, driven by heatshock promoter hsp70.DepositorInsertPcyA and HO1
UseSynthetic Biology; Zebrafish tol2 transposonTagsMTS (Mitochondrial Targeting Signal from subunit …ExpressionMammalianPromoterhsp70Available SinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
3021_pETcon-SARS-CoV-2-RBD_K417N_E484K_N501Y
Plasmid#184406Purposeyeast surface display of the SARS-CoV-2 Beta variant RBDDepositorInsertSARS-CoV-2 Beta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
TagsHA and c-MycExpressionYeastMutationK417N+E484K+N501YAvailable SinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-human-BNIP3 delta Exon2
Plasmid#100782PurposeMammalian expression of BNIP3 delta Exon2 splice variantDepositorInserthuman-BNIP3 delta Exon2 (BNIP3 Human)
TagsHAExpressionMammalianMutationExon2 deletionPromoterCMVAvailable SinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-TPD54(L53P,L67P)
Plasmid#172458PurposeExpression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal mCherry-FKBP tag L53P,L67P mutantDepositorInsertTPD54 (TPD52L2 Human)
TagsmCherry-FKBPExpressionMammalianMutationchanged Leucines 53 and 67 to proline (breaks coi…PromoterCMVAvailable SinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
tRNA-gRNA position [2_n-1] (GB1206)
Plasmid#75407PurposetRNA and scaffold for the assembly of GBoligomers for the intermediate position (positon [2_n-1]) of a polycistronic tRNA-gRNA (3-part multiplexing)DepositorInserttRNA-gRNA position [2_n-1]
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
AIDx-ID-1058
Plasmid#135347PurposeExpression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 1058 of nSpyCas9 with uracil DNA glycosylase inhibitor (UGI) at the C terminusDepositorInsertnSpyCas9-hAIDx-ID-1058-UGI
UseCRISPRExpressionMammalianMutationWTAvailable SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
delNC C-His Ana GvpC in pET28a
Plasmid#85736PurposeExpresses a truncated His-tagged GvpC from Anabaena flos-aquae without the N-and C-terminal regions in E.coliDepositorInsertGas vesicle protein C (truncated and his-tagged)
TagsHis-tagExpressionBacterialMutationDeletion of N and C terminal regions, addition of…PromoterT7Available SinceMarch 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABEMAX-NLS-P2A_BLAST
Plasmid#135355PurposeExpression of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistanceDepositorInsertABEMax-2XNLS
UseCRISPRExpressionMammalianMutationWTAvailable SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMCSF-R(dAB)-luc
Plasmid#12423DepositorInsertM-CSF receptor promoter fragment (CSF1 Human)
UseLuciferaseMutationNo AML1 and C/EBP binding sites (delete bp -86 to…Available SinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only