We narrowed to 8,771 results for: ef1

1. pLENTI EF1alpha FLAG/HA-PALI1

   Plasmid

   #114445

   Purpose

   Mammalian lentiviral expression vector containing N terminal FLAG/HA tagged human PALI1 coding sequence

   Depositor

   Adrian Bracken

   Article

   Conway et al Mol Cell. 2018 May 3;70(3):408-421.e8. d

   Insert

   PALI1 (LCOR Human)
   

   Use

   Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair3

   Plasmid

   #98974

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 37bp 3' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 3 and Cas9(D10A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. LLP109 pEF1a-gRNA-GFP

   Plasmid

   #100935

   Purpose

   GFP, puromycin and gRNA scaffold driven by EF1a promoter

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   gRNA scaffold
   

   Use

   Grna scaffold

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. p156RRL-EF1a-GFP-U3H1SatA

   Plasmid

   #41795

   Depositor

   Inder Verma

   Article

   Zhu et al Nature. 2011 Sep 7;477(7363):179-84. doi

   Insert

   satellite repeat sequence
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB_EF1a-CasRx-msfGFP-2A-Blast

   Plasmid

   #226010

   Purpose

   CasRx protein for RNA targeting in piggyBac backbone

   Depositor

   Silvana Konermann

   Article

   Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

   Insert

   CasRx RNA-targeting nuclease
   

   Use

   CRISPR

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAID1.2-EF1a-NmCherry-mAID

   Plasmid

   #140603

   Purpose

   Expression of OsTIR1 and mCherry-mAID protein in mammalian cells under the control of EF1a promoter

   Depositor

   Tatsuo Fukagawa

   Article

   Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

   Insert

   OsTIR1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPB-EF1a-MegaGate-DD-Blast

   Plasmid

   #175271

   Purpose

   MegaGate destination with C-terminal DD tag and blasticidin resistance

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Tags

   Destabilization Domain

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pOTTC414 - pAAV EF1a hPREP

   Plasmid

   #59967

   Purpose

   An AAV packaging vector that expresses hPREP under control of the EF1a promoter.

   Depositor

   Brandon Harvey

   Article

   Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

   Insert

   Prolyl oligopeptidase (PREP Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRRLNeo-pEF1a-p53ashL344A-mCerulean

   Plasmid

   #69580

   Purpose

   Expresses p53-L344A mutant tagged with mCerulean

   Depositor

   Galit Lahav

   Article

   Gaglia et al Proc Natl Acad Sci U S A. 2013 Sep 17;11

   Insert

   p53-L344A (TP53 Human)
   

   Use

   Lentiviral

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   p53 L344A mutation that prevents tetramerization …

   Promoter

   EF1alpha

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti-EF1alpha-scFv-VP64_Blast

    Plasmid

    #192655

    Purpose

    3rd generation lenti vector encoding scFv-VP64 with 2A Blast resistance marker

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    scFv-VP64
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-Ef1a-DIO_EGFP-RandE3

    Plasmid

    #79872

    Purpose

    For generating adeno-associated virus to express RandE3 under the EF1a promoter in a 'Cre-on' dependent manner.

    Depositor

    Don Arnold

    Article

    Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

    Insert

    GFP-RandE3
    

    Use

    AAV, Cre/Lox, and Synthetic Biology

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. 3xFLAG-dCas9/pTEF1p-CYC1t

    Plasmid

    #62190

    Purpose

    Expresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujii et al Biol Methods Protoc. 2022 Oct 17;7(1):bp

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Yeast

    Mutation

    human codon-optimized, D10A, H840A

    Promoter

    TEF1 promoter

    Available since

    Feb. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-EF1a-DHB-mTurq2

    Plasmid

    #212667

    Purpose

    Express tagged DHB

    Depositor

    Yumi Konagaya, Tobias Meyer

    Article

    Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

    Insert

    DHB (HELB Human)
    

    Use

    Lentiviral

    Tags

    mTurq2

    Expression

    Mutation

    Promoter

    EF-1a

    Available since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAS_4xBstTyrT(CUA)_EF1 sfGFP150TAG

    Plasmid

    #174891

    Purpose

    amber suppression reporter sfGFP150TAG expression, with BstTyr(CUA) amber suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG in sfGFP

    Promoter

    EF1

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMV-SB100X[EF1α-copGFP]

    Plasmid

    #218323

    Purpose

    Donor plasmid of SB100X Transposon with copGFP mammalian expression cassette

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    copGFP mammalian expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDH-EF1-Fon-tdTomato

    Plasmid

    #72261

    Purpose

    Express tdTomato when FLP is expressed by another vector

    Depositor

    Kazuhiro Oka

    Insert

    tdTomato
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    T230I in tdTomato (please see depositor's co…

    Promoter

    elongation factor-1

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-ef1a-DIO-eGCaMP(2+)

    Plasmid

    #201150

    Purpose

    AAV virus production for Cre recombinase dependent expression of eGCaMP(2+)

    Depositor

    Andre Berndt

    Article

    Wait et al Res Sq. 2023 Aug 7:rs.3.rs-3146778. doi:

    Insert

    eGCaMP_2+
    

    Use

    AAV, Cre/Lox, and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEF1α-squirrel-monkey-retroCHMP3-HA

    Plasmid

    #154174

    Purpose

    expresses squirrel monkey retroCHMP3 in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

    Insert

    retroCHMP3
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEF1α-Cre-PGK-Puro

    Plasmid

    #207742

    Purpose

    Co-expression of the Cre recombinase and a puro selectable marker

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    Cre-PGK-Puro
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pAAV-EF1a-flex-TVA

    Plasmid

    #69618

    Purpose

    Express recepter TVA for envA with AAV

    Depositor

    Naoshige Uchida

    Article

    Menegas et al Elife. 2015 Aug 31;4. doi: 10.7554/eLife

    Insert

    Avian sarcoma and leukemia virus receptor 950 (CD320 quail, Synthetic)
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4

    Plasmid

    #98975

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 4 and Cas9(D10A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. MLCK_pLVX-EF1-Cry2-mCherry

    Plasmid

    #64369

    Purpose

    lentiviral expression of Myosin Light Chain Kinase and Cry2-mCherry

    Depositor

    Matthieu Piel

    Insert

    Myosin Light Chain Kinase (MYLK Human)
    

    Use

    Lentiviral

    Tags

    Cry2-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

23. pCDH-EF1a-extGluc-NGFR

    Plasmid

    #104832

    Purpose

    extGluc luciferase for invivo Imaging, NGFR for flourescent detection or separation

    Depositor

    Irmela Jeremias

    Article

    Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

    Inserts

    extGLuc
    NGFR
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Cofilin1_pLVX-EF1-Cry2-mCherry

    Plasmid

    #64370

    Purpose

    lentiviral expression of Cofilin1 and Cry2-mCherry

    Depositor

    Matthieu Piel

    Insert

    Cofilin1 (CFL1 Human)
    

    Use

    Lentiviral

    Tags

    Cry2-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

25. AAVS1-EF1a-mTagRFP-T-CAAX

    Plasmid

    #167973

    Purpose

    AAVS1 targeting repair template

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    AAVS1 (PPP1R12C, AAVS1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mTagRFP-T-CAAX

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pPV-H1-ccdB-mEF1α-RiH

    Plasmid

    #100598

    Purpose

    piggyBac vector for sgRNA cloning

    Depositor

    Akitsu Hotta

    Article

    Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1

    Type

    Empty backbone

    Use

    Piggybac vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human H1 PolIII promoter, human FTH1 promoter, mo…

    Available since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AAV-EF1A-DIO-mRuby2

    Plasmid

    #126215

    Purpose

    Labeling of cre-expressing neurons (red fluorescence)

    Depositor

    Xue Han

    Article

    Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

    Insert

    mRuby2
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCDF1-WT_RASA2-EF1-Puro

    Plasmid

    #114096

    Purpose

    Express RASA2 in mamalian cells

    Depositor

    Yardena Samuels

    Article

    Arafeh et al Nat Genet. 2015 Dec;47(12):1408-10. doi:

    Insert

    RASA2 (RASA2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pPB-EF1a-MegaGate-DD-Hygro

    Plasmid

    #175267

    Purpose

    MegaGate destination with C-terminal DD tag and hygromycin resistance

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Tags

    Destabilization Domain

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV/EF1a-DIO-SCLM

    Plasmid

    #216759

    Purpose

    AAV2 transfer vector for Cre recombinase-dependent expression of SuperClomeleon from the human elongation factor 1a promoter

    Depositor

    George Augustine

    Insert

    SuperClomeleon
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    S30R and 11 AA deletion in the Cerulean CFP moiet…

    Promoter

    EF1a (human elongation factor 1 alpha)

    Available since

    April 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. IF101: pMVP (L1-L4) EF1a promoter

    Plasmid

    #121687

    Purpose

    pMVP L1-L4 entry plasmid, contains EF1a promoter for 3-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    EF1a promoter
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. miniTol2 x EF1a_EKAREN5 + PGK-blast

    Plasmid

    #167818

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5
    

    Use

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pPB-EF1a-BCL2-hygro

    Plasmid

    #222581

    Purpose

    PiggyBac transposon plasmid for constitutive expression of BCL2

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    BCL2 (BCL2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV-EF1a-PGD2-1.1

    Plasmid

    #214764

    Purpose

    AAV packaging plasmid for GRAB PGD2-1.1 fluorescent sensor under EF1alpha promoter

    Depositor

    Yulong Li

    Article

    Sang et al Cell. 2023 Dec 7;186(25):5500-5516.e21.

    Insert

    GRAB-PGD2-1.1 sensor
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF-1-alpha promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-EF1a-DIO-HTB

    Plasmid

    #44187

    Depositor

    Edward Callaway

    Insert

    Histone tagged GFP, T2A-TVA-E2A-B19G
    

    Use

    AAV; Adeno-associated vector

    Tags

    GFP

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

36. piggybac-EF1-halo-mTBP

    Plasmid

    #197908

    Purpose

    single-particle tracking for mTBP

    Depositor

    James Zhe Liu

    Article

    Dong et al bioRxiv 2022.10.26.513917

    Insert

    TBP (Tbp Mouse)
    

    Use

    Tags

    Halotag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1

    Plasmid

    #98972

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNA targeting hLMNA. Generates breaks with 44bp 5' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 1 and Cas9(D10A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair2

    Plasmid

    #98973

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 96bp 5' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 2 and Cas9(D10A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. EF1a-TALEN_HD (SPC right)

    Plasmid

    #126695

    Purpose

    right TALENS for introducing a double strand break into the genomic human SFTPC locus at the ATG start site

    Depositor

    Darrell Kotton

    Article

    Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

    Insert

    human SPC right
    

    Use

    TALEN

    Tags

    NLS, truncated N term and TAL, FokI

    Expression

    Mammalian

    Mutation

    R451P in SPC

    Promoter

    EF1

    Available since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. EF1a-TALEN_NN (SPC left)

    Plasmid

    #126694

    Purpose

    left TALENS for introducing a double strand break into the genomic human SFTPC locus at the ATG start site

    Depositor

    Darrell Kotton

    Article

    Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

    Insert

    human SPC left
    

    Use

    TALEN

    Tags

    0.5 repeat NN, TAL, FokI and NLS, truncated N ter…

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge