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LAMP1-mRFP-FLAG

Plasmid

#34611

Purpose

3rd gen lentiviral vector expressing a fusion protein of Lamp1, mRFP, and a FLAG tag

Depositor

David Sabatini

Publication

Zoncu et al Science. 2011 Nov 4;334(6056):678-83.

Insert

LAMP1 (Lamp1 Rat)

Use

Lentiviral

Tags

2xFlag and mRFP

Expression

Mammalian

Available since

March 20, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

23 Articles

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Mfn2-YFP

Plasmid

#28010

Depositor

Richard Youle

Publication

Karbowski et al J Cell Biol. 2002 Dec 23. 159(6):931-8.

Insert

mitofusin 2 (MFN2 Human)

Tags

YFP

Expression

Mammalian

Mutation

silent S508S

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pEF1a-BirA-V5-neo

Plasmid

#100548

Purpose

Expresses C-terminal V5-tagged BirA biotin ligase

Depositor

Jian Xu

Publication

Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

Insert

C-terminal V5-tagged BirA

Tags

V5, His

Expression

Mammalian

Promoter

Human EF1a

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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pCW57.1-MAT2A

Plasmid

#100521

Purpose

Dox-repressible cDNA expression of MAT2A (dox-off/ tet-off all-in-one vector)

Depositor

David Sabatini

Publication

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

MAT2A (MAT2A Human)

Use

Lentiviral

Expression

Mammalian

Promoter

pTight TRE

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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FUW-SOKM

Plasmid

#20325

Purpose

Lentiviral plasmid expressing mouse Sox2, Oct4, Klf4 and cMyc for iPS cell generation

Depositor

Rudolf Jaenisch

Publication

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Sox2-P2A-Oct4-T2A-Klf4-E2A-cMyc (Pou5f1, Sox2, Klf4, Myc Mouse)

Use

Lentiviral

Expression

Mammalian

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pCMVHA E2F1

Plasmid

#24225

Purpose

Mammalian expression of human E2F1 with HA tag

Depositor

Kristian Helin

Publication

Lukas et al Mol Cell Biol. 1996 Mar . 16(3):1047-57.

Insert

E2F1 (E2F1 Human)

Tags

HA

Expression

Mammalian

Available since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

31 Articles

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pSFFV_mNG2(11)1-10

Plasmid

#82610

Purpose

Lentiviral vector of constitutive expression of mNeonGreen2(1-10)

Depositor

Bo Huang

Publication

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

mNeonGreen2(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

K128M, S142T, R150M, G172V and K213M

Promoter

SFFV

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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PIK3CA-WT

Plasmid

#16643

Depositor

Bert Vogelstein

Publication

Samuels et al Science. 2004 Apr 23. 304(5670):554.

Insert

PIK3CA (PIK3CA Human)

Tags

Flag

Expression

Mammalian

Available since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pcDNA4-Vps34-Flag

Plasmid

#24398

Depositor

Qing Zhong

Publication

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Vps34 (PIK3C3 Human)

Tags

3xFLAG

Expression

Mammalian

Available since

April 1, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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Human Beta-catenin GFP

Plasmid

#71367

Purpose

mammalian expression of human Beta-catenin GFP

Depositor

Alpha Yap

Publication

Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

Insert

Beta-catenin (CTNNB1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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eeBxb1

Plasmid

#222339

Purpose

Plasmid for expressing evolved and engineered Bxb1 in the mammalian cells

Depositor

David Liu

Publication

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

Codon-optimized evolved and engineered Bxb1

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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lenti_dCas9-KRAB-MeCP2

Plasmid

#122205

Purpose

Bipartite dCas9 repressor (dCas9-KRAB-MeCP2) in a UCOE-SFFV-dCas9 lentiviral vector.

Depositor

Andrea Califano

Publication

Tan et al bioRxiv 2021.06.28.449297

Insert

dCas9-KRAB-MeCP2

Use

Lentiviral

Tags

KRAB-MeCP2

Expression

Mammalian

Mutation

Cas9 (D10A, H840A)

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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PPRE X3-TK-luc

Plasmid

#1015

Purpose

PPAR reporter. 3xDR1 sites upstream of a luciferase reporter.

Depositor

Bruce Spiegelman

Publication

Kim et al Proc Natl Acad Sci U S A 1998 Apr 14;95(

Insert

PPRE

Use

Luciferase

Expression

Mammalian

Available since

Nov. 3, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

98 Articles

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pCMV-PE7

Plasmid

#214812

Purpose

Mammalian expression of SpCas9 PE7 prime editor

Depositor

Brittany Adamson

Publication

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Promoter

CMV

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pmScarlet3_C1

Plasmid

#189753

Purpose

C1 Cloning vector for mammalian cells incorporating mScarlet3 red fluorescent protein

Depositor

Dorus Gadella

Publication

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet3

Expression

Mammalian

Promoter

CMV

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pEF1a-FB-dCas9-puro

Plasmid

#100547

Purpose

Expresses N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9 protein

Depositor

Jian Xu

Publication

Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

Inserts

N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9
puromycin

Use

CRISPR

Tags

FLAG, Biotin-acceptor-site (Avitag)

Expression

Mammalian

Promoter

Human EF1a

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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FUW-tetO-MCS

Plasmid

#84008

Purpose

doxycycline-inducible lentiviral vector

Depositor

Stefano Piccolo

Publication

Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5

Has service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-CMV

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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dCas9-KRAB

Plasmid

#110820

Purpose

Conventional dCas9 repressor-dCas9-KRAB

Depositor

Alejandro Chavez, George Church

Publication

Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

Insert

dCas9-KRAB

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CMV

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pET His6 GST TEV LIC cloning vector (1G)

Plasmid

#29655

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, GST, and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pBPGUw

Plasmid

#17575

Depositor

Gerald Rubin

Publication

Pfeiffer et al Tools for neuroanatomy and neurogenetics

Insert

GAL4 (GAL4 Budding Yeast)

Expression

Insect

Available since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

43 Articles

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