159,557 results

1. pFLAG-GATA2

   Plasmid

   #1418

   Depositor

   Gokhan Hotamisligil

   Article

   Tong et al Mol Cell Biol 2005 Jan;25(2):706-15.

   Insert

   GATA-2 (GATA2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSM-mStayGold

   Plasmid

   #234317

   Purpose

   codon-optimized mStayGold with synthetic introns for the expression in C. elegans

   Depositor

   Kota Mizumoto

   Article

   Ko et al MicroPubl Biol. 2025 Jan 18;2025:10.1791

   Insert

   C. elegans codon optimized mStayGOld
   

   Use

   Tags

   mStayGold

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti-EGFP-SnxA

   Plasmid

   #232460

   Purpose

   Lentiviral EGFP lipid sensor for PI(3,5)P2

   Depositor

   Ken-Ichi Takemaru

   Insert

   EGFP-SnxA
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   EF1 alpha

   Available since

   March 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AiP12799 - pAAV-AiE0170h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2799)

   Plasmid

   #230132

   Purpose

   AiE0170h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

   Depositor

   The Allen Institute for Brain Science, Boaz Levi

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minBG

   Available since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AiP12839 - pAAV-AiE0589m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2839)

   Plasmid

   #230136

   Purpose

   AiE0589m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

   Depositor

   The Allen Institute for Brain Science, Boaz Levi

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minBG

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAcBac1-EcTrp-TGA

   Plasmid

   #228780

   Purpose

   aaRS/tRNA system for incorporation of 5HTP within proteins in mammalian cells at TGA codon

   Depositor

   Abhishek Chatterjee

   Article

   Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

   Inserts

   EcTrpRS
   EcWtR
   

   Use

   Baculoviral

   Tags

   Expression

   Mammalian

   Mutation

   TGA suppressor

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET-28-Strep-OPA1(253-960) (SB263)

   Plasmid

   #227612

   Purpose

   Inducible expression of Strep-tagged Opa1(253-960) in E.coli

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Insert

   OPA1 (OPA1 Human)
   

   Use

   Tags

   Twin-StrepTag

   Expression

   Bacterial

   Mutation

   contains aa 253-960 only

   Promoter

   T7

   Available since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CUP1-∆ssCPY*-mGFP in pRS315

   Plasmid

   #221158

   Purpose

   Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker

   Depositor

   Mark Leake

   Insert

   Carboxypeptidase Y (PRC1 Budding Yeast)
   

   Use

   Tags

   mGFP

   Expression

   Yeast

   Mutation

   Deletion signal peptide, G255A mutation

   Promoter

   Available since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CUP1-∆ssCPY*-mScarletI in pRS315

   Plasmid

   #221159

   Purpose

   Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker

   Depositor

   Mark Leake

   Insert

   Carboxypeptidase Y (PRC1 Budding Yeast)
   

   Use

   Tags

   mScarletI

   Expression

   Yeast

   Mutation

   Deletion signal peptide, G255A mutation

   Promoter

   Available since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TadA-NG-CBE

    Plasmid

    #227420

    Purpose

    C-to-T base editing in mammalian cells

    Depositor

    Yang Sun

    Article

    Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

    Insert

    TadA-nSpCas9-NG-2xUGI (AICDA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. WDV-Echistatin

    Plasmid

    #226985

    Purpose

    Expresses the HUH endonuclease WDV fused at the C termini with Echistatin to target RGD binding integrins with DNA tension sensors

    Depositor

    Wendy Gordon

    Article

    Pawlak et al Nat Commun. 2023 Apr 28;14(1):2468. doi:

    Inserts

    WDV (repA )
    Echistatin
    

    Use

    Tags

    6xHis and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pQE81XN-msYFP

    Plasmid

    #218960

    Purpose

    Expresses Hisx6-Tagged msYFP in bacteria

    Depositor

    Benjamin Glick

    Insert

    msYFP
    

    Use

    Tags

    6X-His

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PRS_8-GCaMP8m

    Plasmid

    #210400

    Purpose

    AAV-vector for fluorescent calcium measurements in the green spectrum from noradrenergic neurons

    Depositor

    Simon Wiegert

    Article

    Wissing et al bioRxiv 2022.01.22.477348

    Insert

    GCaMP8m
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PRSx8

    Available since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSpCas9(BB)-2A-Puro-C9orf72

    Plasmid

    #83439

    Purpose

    Expresses Cas9 and a gRNA targeting C9orf72

    Depositor

    Shawn Ferguson

    Article

    Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

    Insert

    gRNA targeting human C9orf72 (C9orf72 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPRKCZ-C1-EGFP (C-PRKCZ-C1)

    Plasmid

    #217759

    Purpose

    Fluorescent reporter for ceramide (putative, mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Protein kinase C zeta (PRKCZ Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C1 domain (aa 123-193)

    Promoter

    CMV

    Available since

    June 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. CXCR1-DuET

    Plasmid

    #213218

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CXCR1 (CXCR1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CCR6-DuET

    Plasmid

    #213202

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CCR6 (CCR6 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CCR3-DuET

    Plasmid

    #213199

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CCR3 (CCR3 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CCR4-DuET

    Plasmid

    #213200

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CCR4 (CCR4 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKD013

    Plasmid

    #207931

    Purpose

    Premade plasma membrane marker, pUBQ10:PiP1;4:mTurquoise2:tHSP18.2M, for braiding

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAS_4xMmaPylT(UCA)_EF1_MmaPylRS

    Plasmid

    #174899

    Purpose

    Mma PylRS expression for opal suppression in mammalian cells; transient or piggy bac mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PJEBAN3

    Plasmid

    #203682

    Purpose

    Expresses Yellow Fluorescent Protein (YFP) in Listeria monocytogenes

    Depositor

    Tine Rask Licht

    Article

    Andersen et al BMC Microbiol. 2006 Oct 3;6:86. doi: 10.

    Insert

    Yellow Fluorescent Protein (YFP)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. iCAP-BI-48-KanR

    Plasmid

    #203533

    Purpose

    RepCap for AAV production

    Depositor

    Ben Deverman

    Article

    Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

    Insert

    AAV-BI48 Cap
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. iCAP-BI-62-KanR

    Plasmid

    #203535

    Purpose

    RepCap for AAV production

    Depositor

    Ben Deverman

    Article

    Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

    Insert

    AAV-BI62 Cap
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMSCV-IRES-GFP-MD2-HA

    Plasmid

    #133065

    Purpose

    Retroviral expression of mouse MD2 tagged with HA

    Depositor

    Jonathan Kagan

    Article

    Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

    Insert

    MD2 (Ly96 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMEH373 dTomato 3x high affinity MS2 loops

    Plasmid

    #168221

    Purpose

    Mammalian expression of dTomato with 3 high affinity MS2 loops

    Depositor

    Joshua Leonard

    Article

    Hung et al J Extracell Vesicles. 2016 May 13;5:3102

    Insert

    dTomato
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMarC9-R6k

    Plasmid

    #89477

    Purpose

    transposon plasmid, compatible with Tn-Seq

    Depositor

    George Church

    Article

    Lee et al Nat Microbiol. 2019 Apr 8. pii: 10.1038/

    Insert

    pMarC9-R6k
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLVX-Tight-Puro_MYRF

    Plasmid

    #64843

    Purpose

    A lentiviral vector for the expression of mouse MYRF under doxycyline control.

    Depositor

    Paul Tesar

    Article

    Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

    Insert

    MYRF (Myrf Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBN42

    Plasmid

    #86713

    Purpose

    C. elegans injection marker

    Depositor

    Peter Askjaer

    Article

    Morales-Martinez et al J Cell Sci. 2015 Mar 15;128(6):1090-6. d

    Insert

    Pmyo-3::GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHCMV-10A1

    Plasmid

    #15805

    Depositor

    Miguel Sena-Esteves

    Article

    Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

    Insert

    10A1 Murine leukemia virus envelope mRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET21-pelB-LaM-4

    Plasmid

    #172770

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-4
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAV.hSyn.FLEX.iGluSnFR3.v857.GPI.codonopt

    Plasmid

    #175181

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Has Service

    AAV1

    Insert

    pAAV hSyn FLEX iGluSnFR3 v857.GPI
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    GPI Anchor, IgK-chain, and Myc epi tag

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMuLE_ENTR_12GLI-FLuc_R4-R3

    Plasmid

    #113712

    Purpose

    Single pathway reporter; hedgehog promoter-driven expression of firefly luciferase; MultiSite Gateway entry vector.

    Depositor

    Manfred Ogris

    Article

    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

    Insert

    12GLI-FLuc
    

    Use

    Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCAG_Xph15-SNAPf-CCR5TC

    Plasmid

    #135536

    Purpose

    Encodes a specific PSD-95 binder (Xph15) fused to SNAPf, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

    Insert

    Xph15
    

    Use

    Tags

    SNAPf

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pDN-Sth1Cas9

    Plasmid

    #221386

    Purpose

    Cas9 under control of AHT inducible TetR on integrating plasmid (KanR) using L5 integrase and attP for site-specific integration into attB

    Depositor

    Deborah Hung

    Article

    Neo et al J Bacteriol. 2024 Mar 21;206(3):e0033523

    Insert

    Sth1 Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAV-mDlx-NLS-mRuby2

    Plasmid

    #99130

    Purpose

    An AAV genome that expresses nuclear localized mRuby2 from the mouse Dlx5/6 enhancer

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV8, and AAV9

    Insert

    NLS-mRuby2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mDLX5/6

    Available since

    Aug. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMAL-c5X-ELP[AV-60]

    Plasmid

    #67012

    Purpose

    Expresses malE-ELP[AV-60] in E. coli

    Depositor

    Ashutosh Chilkoti

    Article

    Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

    Insert

    malE-ELP[AV-60]
    

    Use

    Tags

    His6 and malE-Factor Xa

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLenti-CAG-empty-ires-EGFP

    Plasmid

    #122953

    Purpose

    Lentiviral expression empty vector under CAG promoter with EGFP expression

    Depositor

    Pierre Vanderhaeghen

    Article

    Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCAG-mEGFP-CaMKIIa (T286A)

    Plasmid

    #127390

    Purpose

    Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286A)

    Depositor

    Ryohei Yasuda

    Article

    Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

    Insert

    calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    changed Threonine 286 to Alanine

    Promoter

    pCAG

    Available since

    Jan. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTH10

    Plasmid

    #188783

    Purpose

    Carries a NAT marker and C. albicans RBT5 promoter in a pGEM-T backbone

    Depositor

    Aaron Mitchell

    Insert

    RBT5 promoter
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

41. KRphi-mRFP

    Plasmid

    #17276

    Depositor

    Sergio Grinstein

    Article

    Yeung et al Science. 2006 Jul 21. 313(5785):347-51.

    Insert

    KR phi
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 2, 2008

    Availability

    Academic Institutions and Nonprofits only

42. LEPZ

    Plasmid

    #111161

    Purpose

    miR-E (miR-30 variant)-based RNAi with ZsGreen reporter

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Insert

    miR-E (miR-30 variant)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pGEX4T1-Opt WWP1

    Plasmid

    #179327

    Purpose

    E. coli optimized expression of GST-fusion of human WWP1

    Depositor

    Arthur Haas

    Insert

    WWP1 (WWP1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon optimized for expression in E. coli

    Promoter

    Available since

    April 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pENTR-LUC (w158-1)

    Plasmid

    #17473

    Purpose

    Gateway entry vector, Firefly Luciferase

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    Firefly Luciferase
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pmTurquoise2-Tubulin

    Plasmid

    #36202

    Purpose

    In vivo visualization of microtubules (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    Alpha-tubulin (TUBA1B Human)
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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46. pCMV-PE7-mutant (Q20A_Y23A_Y24F_F35A)

    Plasmid

    #224123

    Purpose

    Mammalian expression of SpCas9 PE7 mutant prime editor (Q20A_Y23A_Y24F_F35A)

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7-mutant (Q20A_Y23A_Y24F_F35A)
    

    Use

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Q20A_Y23A_Y24F_F35A in La(SSB)1-194

    Promoter

    CMV

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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47. pVDL9.3

    Plasmid

    #168299

    Purpose

    Secretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyA

    Depositor

    Víctor de Lorenzo

    Article

    Tzschaschel et al Nat Biotechnol. 1996 Jun;14(6):765-9. do

    Insert

    Segment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    LacI-Plac

    Available since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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48. pJYS3_ΔcrtYf

    Plasmid

    #85542

    Purpose

    Constitutive transcription of FnCpf1 and crRNA of crtYf

    Depositor

    Sheng Yang

    Article

    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

    Inserts

    Cpf1
    crRNA of crtYf
    

    Use

    CRISPR; Shuttle vector corynebacterium glutamicum…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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49. pUB_smHA_KDM5B_MS2

    Plasmid

    #81085

    Purpose

    Translation reporter: Encodes KDM5B (1544 aa) fused to smHA (Spaghetti Monster HA: 10 copies of an epitope tag HA).

    Depositor

    Tim Stasevich

    Article

    Morisaki et al Science. 2016 Jun 17;352(6292):1425-9. d

    Insert

    smHA_KDM5B_24xMS2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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50. pICSL01009::AtU6p

    Plasmid

    #46968

    Purpose

    Encodes the Arabidopsis U6 promoter in a level 0 vector (Weber et al. PLoS One 6:e16765, 2011). It is used to place an sgRNA uder the Arabidopsis U6 promoter.

    Depositor

    Sophien Kamoun

    Article

    Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

    Insert

    AtU6p
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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