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169,677 results

Showing: 11551 - 11600 of 169677 results

  1. pAAV.CMV.HI.eGFP-Cre.WPRE.SV40

    Plasmid
    #105545
    Purpose
    AAV expression of EGFP-Cre from CMV promoter
    Depositor
    James M. Wilson
    Has Service
    AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    EGFP-Cre
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Promoter
    HI
    Available Since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105545-plasmid-map-sequence-id-202462

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  7. pUCM-CLYBL-NGN2-BRN3A

    Plasmid
    #165597
    Purpose
    Donor construct for insertion of NGN2 and BRN3A into the CLYBL safe harbor site. This is an all-in-one doxycycline-inducible construct, enabling iPSC differentiation into peripheral sensory neurons.
    Depositor
    Carsten Bönnemann
    Article
    Nickolls et al Cell Rep. 2020 Jan 21;30(3):932-946.e7.
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    BRN3A (POU4F1 Human)
    EGFP
    Puromycin N-acetyl transferase
    rtTA3G
    SBP Delta-LNGFR
    Use
    CRISPR and TALEN
    Expression
    Mammalian
    Promoter
    CAG, EF-1alpha, Endogenous CLYBL (splice acceptor…
    Available Since
    March 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    165597-plasmid-map-sequence-id-326012

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  14. pBA900

    Plasmid
    #122237
    Purpose
    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs2 incorporated at the 3' end of the sgRNA constant region.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA with cs2 at the 3' end of the constant region
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    122237-plasmid-map-sequence-id-292010

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  21. SaCas9v3-Puro

    Plasmid
    #178813
    Purpose
    SaCas9 with 2A-Puro, and a cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available Since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178813-plasmid-map-sequence-id-354841

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  24. pLV-UbC-Tet1v4-dCas9

    Plasmid
    #232180
    Purpose
    Tet1v4-dCas9 from Nuñez et al. 2021, in a lentiviral cassette with blasticidin resistance, for expression in mammalian cells
    Depositor
    Charles Gersbach
    Article
    Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi
    Insert
    Tet1 catalytic domain - XTEN80 linker - dead Cas9 - 2A - BlastR (TET1 S. pyogenes, Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    hUbC
    Available Since
    March 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    232180-plasmid-map-sequence-id-462961

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  29. pcDNA3.1-Bsd-mEGFP

    Plasmid
    #214369
    Purpose
    Expression of the monomeric Green Fluorescent Protein
    Depositor
    Bertrand Collet
    Insert
    mEGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    214369-plasmid-map-sequence-id-423969

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  34. pcDNA3.1+ eiF4A1 myc HIS

    Plasmid
    #71657
    Purpose
    mammalian expression of eiF4A1
    Depositor
    Gideon Dreyfuss
    Insert
    eiF4A1 (EIF4A1 Human)
    Tags
    HIS and myc
    Expression
    Mammalian
    Mutation
    aa403 from Ala to Arg
    Promoter
    CMV
    Available Since
    Jan. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71657-plasmid-map-sequence-id-422687

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  37. CRISPRoff-mScarletI

    Plasmid
    #217783
    Purpose
    Expresses CRISPRoff-mScarletI (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-mScarletI-KRAB) downstream of the CAG promoter for gene epigenetic silencing
    Depositor
    Adriano Aguzzi
    Article
    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038
    Insert
    CRISPRoff-mScarletI (DNMT3A-DNMT3L-dCas9-mScarletI-KRAB)
    Tags
    2xNLS, DNMT3A-DNMT3L, HA, KRAB, and mScarletI
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    April 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    217783-plasmid-map-sequence-id-468886

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  38. pMAL-c2X_MBP-CLmN-H6

    Plasmid
    #234487
    Purpose
    Maltose binding protein N-terminally fused to His-tagged CLmN split intein
    Depositor
    Henning Mootz
    Article
    Humberg et al Nat Commun. 2025 Mar 19;16(1):2723. doi:
    Insert
    N-terminal Fragment of the CLm Split Intein
    Tags
    Hexahistidine Tag and Maltose Binding Protein (MB…
    Expression
    Bacterial
    Mutation
    T69K, F75H, M118N within the N-terminal fragment …
    Available Since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    234487-plasmid-map-sequence-id-471039

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  39. pD649-HAsp-LILRB2-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156863
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    LILRB2 (LILRB2 Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    Jan. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    156863-plasmid-map-sequence-id-382983

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  41. BB3cN_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104911
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Jan. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    104911-plasmid-map-sequence-id-269032

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  42. pS34.EF1α<GFP-2A-Cas9-RC

    Plasmid
    #207404
    Purpose
    Cas9-RC and GFP from an EF1α promoter. High-performance knockin through DSB repair by homology-directed recombination (HDR) or homology-mediated end joining (HMEJ). [Lab plasmid ID: N213]
    Depositor
    Alexandros Poulopoulos
    Article
    Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10
    Insert
    Cas9-RC
    Use
    CRISPR and Synthetic Biology
    Tags
    GFP-2A
    Expression
    Mammalian
    Promoter
    EF1α
    Available Since
    Nov. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    207404-plasmid-map-sequence-id-413204

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  47. EF1α-mNeonGreen-Z1

    Plasmid
    #135613
    Purpose
    mNeonGreen monomeric fluorescent protein expression for mammalian cells under EF1a-HTLV promoter in minimal backbone plasmid.
    Depositor
    Jordan Green
    Insert
    mNeonGreen
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF1a-HTLV
    Available Since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135613-plasmid-map-sequence-id-269406

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  48. pAG mIgG2a Anti-c-Myc [9E10]

    Plasmid
    #242955
    Purpose
    Mammalian expression plasmid for Anti-c-Myc [9E10] fused to mouse IgG2a heavy chain constant region; to be used with pAG mKappa Anti-c-Myc light chain [9E10] (Plasmid 242956) to make the antibody.
    Depositor
    Meghan Rego, Addgene Research Program
    Insert
    Anti-c-Myc [9E10] fused to mouse IgG2a heavy chain
    Tags
    Mouse IgG2a
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    242955-plasmid-map-sequence-id-484469

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  49. pAG mKappa Anti-c-Myc [9E10]

    Plasmid
    #242956
    Purpose
    Mammalian expression plasmid for Anti-c-Myc [9E10] fused to mouse kappa light chain constant region; to be used with pAG mIgG2a Anti-c-Myc [9E10] heavy chain (Plasmid 242955) to make the antibody.
    Depositor
    Meghan Rego, Addgene Research Program
    Insert
    Anti-c-Myc [9E10] light chain variable region fused to mouse kappa light chain constant region
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    242956-plasmid-map-sequence-id-485246

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  50. pENTR_mCherry

    Plasmid
    #139554
    Purpose
    mCherry ORF with stop codon between attL1-attL2 on Gateway entry vector
    Depositor
    Minoru Ko
    Insert
    mCherry
    Expression
    Mammalian
    Available Since
    March 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    139554-plasmid-map-sequence-id-274727

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Showing: 11551 - 11600 of 169677 results