Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

172,031 results

Showing: 11551 - 11600 of 172031 results

  2. pMS2M-PH-gagpol-D64V

    Plasmid
    #166031
    Purpose
    Expression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at N terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.
    Depositor
    Yujia Cai
    Article
    Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038
    Insert
    MS2-Gag-Pol
    Use
    Lentiviral
    Mutation
    D64V mutation within the integrase within Pol
    Available Since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166031-plasmid-map-sequence-id-328290

    Enlarge

  3. pWJ68

    Plasmid
    #46571
    Purpose
    Same as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9)
    Depositor
    Luciano Marraffini
    Article
    Bikard et al Nucleic Acids Res. 2013 Jun 12.
    Inserts
    tracrRNA
    w-dcas9
    CRISPR array
    Use
    CRISPR
    Tags
    rpoZ
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Available Since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    46571-plasmid-map-sequence-id-65847

    Enlarge

  4. pENTR-R2L3-IRES-Zeo-pA

    Plasmid
    #139530
    Purpose
    Provides IRES-Zeo-pA cassette on Gateway entry vector
    Depositor
    Minoru Ko
    Insert
    Zeocin resistant gene
    Expression
    Mammalian
    Available Since
    March 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    139530-plasmid-map-sequence-id-274699

    Enlarge

  12. pAAV-CaMKIIa-hM4D(Gi)-mCherry

    Plasmid
    #50477
    Purpose
    Gi-coupled hM4D DREADD fused with mCherry under the control of CaMKIIa promoter
    Depositor
    Bryan Roth
    Has Service
    AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    hM4D(Gi)-mCherry (CHRM4 Human)
    Use
    AAV
    Tags
    mCherry
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    CaMKIIa
    Available Since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    50477-plasmid-map-sequence-id-370683

    Enlarge

  19. 5KO9 (HMCES)

    Plasmid
    #101877
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HMCES (HMCES Human)
    Tags
    6x His (modified C-term tag is AENLYFQSHHHHHH)
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 30, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    101877-plasmid-map-sequence-id-194533

    Enlarge

  31. TFORF2952

    Plasmid
    #144428
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TLE1 (TLE1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    144428-plasmid-map-sequence-id-391591

    Enlarge

  32. lentiCas9n(D10A)-Blast

    Plasmid
    #63593
    Purpose
    Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Inserts
    Cas9
    Blasticidin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D10A, nickase mutant
    Promoter
    EFS-NS
    Available Since
    April 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63593-plasmid-map-sequence-id-181681

    Enlarge

  37. pLV-REPORT(PGK/CMV)

    Plasmid
    #172330
    Purpose
    LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an PGK/CMV promoter driving nuclear d2eGFP E2A Hygromycin
    Depositor
    David Bersten
    Article
    Allen et al bioRxiv 2023.11.21.568191
    Inserts
    monomeric nuclear Tomato
    d2eGFP
    HygR
    Use
    Lentiviral and Synthetic Biology
    Tags
    3x SV40 NLS
    Expression
    Mammalian
    Promoter
    PGK/CMV
    Available Since
    June 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    172330-plasmid-map-sequence-id-497743

    Enlarge

  39. TRUPATH Triple GaoB

    Plasmid
    #196052
    Purpose
    Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaoB-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196052-plasmid-map-sequence-id-387470

    Enlarge

  40. Tet-O-FUW-FOXA2-PURO

    Plasmid
    #251737
    Purpose
    Overexpression construct expressing FOXA2-PURO and ORF-BC
    Depositor
    Andrea Califano
    Insert
    FOXA2 (FOXA2 Human)
    Use
    Lentiviral
    Available Since
    March 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    251737-plasmid-map-sequence-id-501493

    Enlarge

  42. TtEnd5A_pEHISTEV

    Plasmid
    #179732
    Purpose
    TtEnd5A_pEHISTEV expression vector encodes first Thermogutta terrifontis Endoglucanase (19-531 amino acid residues) of Glycoside Hydrolase Family 5.
    Depositor
    James Naismith
    Article
    Hussain et al Arch Biochem Biophys. 2025 Feb;764:11027
    Insert
    TtEnd5A
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    19 Amino acids from N-terminal encoding Signal pe…
    Promoter
    T7
    Available Since
    Oct. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    179732-plasmid-map-sequence-id-357162

    Enlarge

  43. NRF2-SmBiT_MAFG-LgBiT BiBiT Vector

    Plasmid
    #238773
    Purpose
    Express NRF2-SmBiT_MAFG-LgBiT Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Inserts
    NRF2 (NFE2L2 Human)
    MAFG (MAFG Human)
    Tags
    LgBiT and SmBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238773-plasmid-map-sequence-id-479907

    Enlarge

  44. HaloTag-JAK3 Vector

    Plasmid
    #238701
    Purpose
    Express HaloTag-JAK3 Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    JAK3 (JAK3 Human)
    Tags
    HaloTag (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238701-plasmid-map-sequence-id-479800

    Enlarge

  46. NanoBRET Assay Vector, NanoLuc-MLL1

    Plasmid
    #237135
    Purpose
    Express NanoLuc(R)-MLL1 Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    MLL1 (KMT2A Human)
    Tags
    NanoLuc (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237135-plasmid-map-sequence-id-476791

    Enlarge

Showing: 11551 - 11600 of 172031 results