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We narrowed to 14,145 results for: TIM

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Showing: 11601 - 11650 of 14145 results

  1. pFGL1252_TetOFF(Hyg)

    Plasmid
    #118992
    Purpose
    TetOFF (promoter-less) cassette with Hygromycin selection
    Depositor
    Naweed Naqvi
    Article
    Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581
    Inserts
    tTA+Tnos
    Hygromycin cassette
    Ttub+PtetO
    Use
    Fungal expression (in magnaporthe oryzae)
    Mutation
    Codon optimized for Magnaporthe oryzae
    Promoter
    Aspergillus nidulans TrpC promoter, Ustilago mayd…
    Available Since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    118992-plasmid-map-sequence-id-232118

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  5. 3xFLAG-dCas9/pMSCVneo

    Plasmid
    #134982
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available Since
    April 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    134982-plasmid-map-sequence-id-430627

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  6. pMOD_B2515b

    Plasmid
    #91073
    Purpose
    Module B, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, structurally optimized gRNA scaffold, Terminator: Pol III
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning
    Use
    CRISPR
    Promoter
    AtU6
    Available Since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91073-plasmid-map-sequence-id-181900

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  7. pHS1543

    Plasmid
    #205963
    Purpose
    NZ_CP025815 DinG HNH Cascade proteins operon (E. coli codon optimized) with His14-tagged Csf3 in pET45b(+)
    Depositor
    Feng Zhang
    Article
    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.
    Insert
    DinG HNH Cascade with His14-tagged Csf3
    Tags
    14xHis on Csf3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    205963-plasmid-map-sequence-id-415370

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  10. pBT3024

    Plasmid
    #122542
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a mitochondrial target peptide (mt) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_mt::CemOrange2
    Tags
    MLSLRQSIRFFKPATRTLCSSRTLL
    Expression
    Bacterial
    Promoter
    Pnhx-2
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122542-plasmid-map-sequence-id-238125

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  11. pAH-CTX1-rhadCas9-native

    Plasmid
    #129392
    Purpose
    Derived from pAH-CTX1-rha. The native Streptococcus pyogenes dCas9 gene cloned downstream of PrhaBAD. Non-codon-optimized dCas9 version of pAH-CTX1-rhadCas9.
    Depositor
    Silvia Cardona
    Article
    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021
    Insert
    S. pyogenes dCas9
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Aspartate 10 to Alanine, Histidine 840 to Alanine
    Promoter
    PrhaBAD
    Available Since
    Dec. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129392-plasmid-map-sequence-id-266824

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  20. MSP2253

    Plasmid
    #70704
    Purpose
    Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    T7
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70704-plasmid-map-sequence-id-127764

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  24. MSP3-bio

    Plasmid
    #47731
    Purpose
    Expresses enzymatically monobiotinylated full-length MSP3 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised MSP3
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47731-plasmid-map-sequence-id-68466

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  26. MSP2-bio

    Plasmid
    #47710
    Purpose
    Expresses enzymatically monobiotinylated full-length MSP2 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised MSP2
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47710-plasmid-map-sequence-id-68424

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  33. pCDNA-CRY2-mCh-superPLDx30-P2A-CIBN-CAAX

    Plasmid
    #188992
    Purpose
    A plasmid for dual expression of CRY2-mCh-superPLDx30 (an engineered PLD mutant with 30 times higher activity than the wild-type) and plasma membrane-tagged CIBN
    Depositor
    Jeremy Baskin
    Article
    Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415
    Insert
    PLD
    Tags
    CAAX, CIBN, CRY2, P2A, and mCherry
    Expression
    Mammalian
    Mutation
    K109R, P245A, G328S, G381V, G429D
    Promoter
    CMV
    Available Since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    188992-plasmid-map-sequence-id-371645

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  35. pCXLE-dCas9VPPH-T2A-GFP-shP53

    Plasmid
    #102897
    Purpose
    EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    dCas9VPPH-T2A-GFP-shP53
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available Since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102897-plasmid-map-sequence-id-194735

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  42. evoCas9

    Plasmid
    #138558
    Purpose
    Expresses human codon-optimized evolved SpCas9 and blasticidin resistance: EFS promoter-evoCas9-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    evoCas9
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    M495V, Y515N, K526E, R661Q
    Promoter
    EFS
    Available Since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138558-plasmid-map-sequence-id-274774

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  47. pC1300_pUB10_pco-vCASphi_E9t_V2_pUB10_E9t_ribozyme_AtPDS3_gRNA10

    Plasmid
    #197979
    Purpose
    T-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by UBQ10 gene promoter, flanked by ribozymes.
    Depositor
    Steven Jacobsen
    Article
    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12
    Inserts
    pco-vCasphi-2
    AtPDS3 gRNA10
    Use
    CRISPR
    Expression
    Plant
    Promoter
    pUBQ10
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    197979-plasmid-map-sequence-id-388023

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Showing: 11601 - 11650 of 14145 results