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167,150 results

Showing: 11851 - 11900 of 167150 results

  1. Dendra2-NS3

    Plasmid
    #238004
    Purpose
    Expresses NS3 in mammalian/mouse cell line
    Depositor
    Partha Mondal
    Insert
    NS3
    Tags
    Dendra2
    Expression
    Mammalian
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    238004-plasmid-map-sequence-id-474227

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  5. KL094 - pTRE3G-Flag-Halo-CTCF_WT-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor

    Plasmid
    #232930
    Purpose
    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible Flag-Halo-mCTCF WT, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycin selection.
    Depositor
    Danny Reinberg
    Article
    Lucero et al bioRxiv, March 19, 2025
    Insert
    Flag-Halo-CTCF WT
    Use
    Mouse Targeting
    Expression
    Mammalian
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    232930-plasmid-map-sequence-id-466502

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  7. Anti-SAP102 [N19/2-rat IgG2a] - Chimeric

    Plasmid
    #227076
    Purpose
    Mammalian expression plasmid of anti-SAP102 (Rattus norvegicus) rat IgG2a R-mAb. Derived from hybridoma N19/2.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Anti-SAP102 (Rattus norvegicus) recombinant mouse monoclonal antibody. (Dlg3 Chimera: M. musculus (mouse)/R. norvegicus (rat))
    Use
    Affinity Reagent/ Antibody
    Expression
    Mammalian
    Promoter
    Dual CMV
    Available Since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    227076-plasmid-map-sequence-id-450725

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  8. pCLAP

    Plasmid
    #200786
    Purpose
    Cloning template for N-terminal CLIPf tagging and C-terminal SNAPf tagging of constructs for mammalian expression. Also used for the mammalian expression of CLIPf-SNAPf-HisTag-FLAG fusion protein.
    Depositor
    Alison Twelvetrees
    Article
    Smith et al bioRxiv 2024.12.20.629623
    Type
    Empty backbone
    Tags
    CLIPf and SNAPf-6xHis-FLAG
    Expression
    Mammalian
    Available Since
    Dec. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    200786-plasmid-map-sequence-id-435692

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  9. AiP13655 - pAAV-AiE0552h-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN3655)

    Plasmid
    #224035
    Purpose
    AiE0552h is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_IT and L6_IT neurons
    Depositor
    Allen Institute for Brain Science, Jonathan Ting
    Article
    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.
    Insert
    SYFP2-P2A-3XFLAG-10aa-H2B
    Use
    AAV
    Mutation
    NA
    Promoter
    minBG
    Available Since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    224035-plasmid-map-sequence-id-449734

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  13. pETM11-6xHis-TEV-hORF1p_R261A

    Plasmid
    #202586
    Purpose
    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the R261A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    ORF1p
    Tags
    6x His and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Changed ORF1p Arginine 261 to Alanine
    Available Since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    202586-plasmid-map-sequence-id-415635

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  16. TFORF0712

    Plasmid
    #143067
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF626 (ZNF626 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143067-plasmid-map-sequence-id-399055

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  17. TFORF3409

    Plasmid
    #144885
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF621 (ZNF621 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144885-plasmid-map-sequence-id-353335

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  21. LSB-hsa-miR-3074-5p

    Plasmid
    #103408
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-3074-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-3074-5p target (MIR3074 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103408-plasmid-map-sequence-id-195865

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  30. AICSDP-10:LMNB1-mEGFP

    Plasmid
    #87422
    Purpose
    Homology arms and linker-mEGFP sequence for N-terminus tagging of human LMNB1
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    LMNB1 Homology Arms with mEGFP-linker (LMNB1 Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87422-plasmid-map-sequence-id-173949

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  41. pAAV-anti-PDL1(KN035) PAGER(TF)

    Plasmid
    #230003
    Purpose
    Expresses anti-PDL1 PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)
    Depositor
    Alice Ting
    Article
    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-
    Insert
    IL2SP-Aro6-KN035-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
    Use
    AAV
    Expression
    Mammalian
    Mutation
    V360A/R361A on KORD
    Promoter
    CMV
    Available Since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230003-plasmid-map-sequence-id-457206

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  44. dTAG-IRF8

    Plasmid
    #190620
    Purpose
    Lentiviral expression plasmid of human IRF8 fused with FKBP12G36V for dTAG-mediated degradation
    Depositor
    Junwei Shi
    Article
    Cao et al Mol Cell. 2021 Sep 2;81(17):3604-3622.e1
    Insert
    IRF8 (IRF8 Human)
    Use
    Lentiviral
    Tags
    FKBP12_G36V
    Expression
    Mammalian
    Mutation
    synonymous mutation for sgRNA resistance for sgIR…
    Available Since
    Oct. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    190620-plasmid-map-sequence-id-372992

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Showing: 11851 - 11900 of 167150 results