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177,138 results

Showing: 11851 - 11900 of 177138 results

  1. pLenti6.3/EV

    Plasmid
    #120848
    Purpose
    Control vector, 20nt random sequence between gateway recombination sites
    Depositor
    Jan Rehwinkel
    Type
    Empty backbone
    Use
    Lentiviral
    Available since
    Feb. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    120848-plasmid-map-sequence-id-234653

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  3. pGEX6P1-N-HA

    Plasmid
    #119756
    Purpose
    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal HA tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Tags
    GST and HA
    Expression
    Bacterial
    Promoter
    tac promoter
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    119756-plasmid-map-sequence-id-233665

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  4. pGEX6P1-N-His

    Plasmid
    #119755
    Purpose
    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal 10x His tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Tags
    10xHis and GST
    Expression
    Bacterial
    Promoter
    tac promoter
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    119755-plasmid-map-sequence-id-233662

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  6. pAAV-HyPer-DAAO-NES

    Plasmid
    #119164
    Purpose
    Fusion of hydrogen peroxide fluorescent biosensor HyPer and D-amino acid oxidase; excluded from nucleus. Allows H2O2 production/detection by adding D-amino acids. AAV expression vector; CMV promoter.
    Depositor
    Thomas Michel
    Publication
    Steinhorn et al Nat Commun. 2018 Oct 2;9(1):4044. doi: 1
    Insert
    HyPer-DAAO-NES
    Use
    AAV
    Tags
    NES
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    119164-plasmid-map-sequence-id-232464

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  9. pGDP2 MCR-1

    Plasmid
    #118404
    Purpose
    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the pLac promoter.
    Depositor
    Gerard Wright
    Publication
    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109
    Insert
    MCR-1
    Expression
    Bacterial
    Promoter
    pLac
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    118404-plasmid-map-sequence-id-229034

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  21. dxCas(3.6)-VPR

    Plasmid
    #108388
    Purpose
    Mammalian expression vector for xCas9 3.6 with VP64-p65-Rta (VPR) fused to its C-terminus
    Depositor
    David Liu
    Publication
    Hu et al Nature. 2018 Feb 28. pii: nature26155. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dxCas(3.6)-VPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …
    Available since
    March 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    108388-plasmid-map-sequence-id-207184

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  23. pWS172

    Plasmid
    #90519
    Purpose
    Cas9 gap repair vector - HIS3
    Depositor
    Tom Ellis
    Insert
    Cas9
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Feb. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    90519-plasmid-map-sequence-id-287208

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  25. pRluc-LC3G120A

    Plasmid
    #105003
    Purpose
    Mammalian expression of Rluc-LC3G120A. Can be used for measuring autophagic flux.
    Depositor
    Marja Jããttelã
    Publication
    Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LC3B (Map1lc3b Rat)
    Use
    Luciferase
    Tags
    Rluc with C124A substitution
    Expression
    Mammalian
    Mutation
    Glycine 120 mutated to alanine, prevents lipidati…
    Available since
    Jan. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    105003-plasmid-map-sequence-id-202164

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  28. pAAV-CMV-FLEX-TVAmCherry-2A-oG

    Plasmid
    #102985
    Purpose
    AAV expressing TVA-mCherry fusion and optimised Rabies Glycoprotein (oG) after CRE-mediated recombination
    Depositor
    Marco Tripodi
    Publication
    Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306
    Inserts
    TVA-mCherry fusion protein after CRE-mediated recombination
    Optimised Rabies Glycoprotein (oG) after CRE-mediated recombination
    Use
    AAV; Adeno associated viral vector
    Available since
    Dec. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    102985-plasmid-map-sequence-id-199982

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  30. pINDUCER dCas9-TET1CDMut

    Plasmid
    #101920
    Purpose
    The dCas9-TET1CDMut fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948). The TET1 catalytic domain is mutated to abolish catalytic function.
    Depositor
    Danwei Huangfu
    Publication
    Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10
    Insert
    dCas9-TET1CD Mut
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Mutated TET1 catalytic domain
    Available since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    101920-plasmid-map-sequence-id-194554

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  39. pmCherry-N1-GalT

    Plasmid
    #87327
    Purpose
    To express GalT-mCherry in mammalian cells, GalT encodes the 1-81 amino acids from β1,4-galactosyltransferase
    Depositor
    Lei Lu
    Publication
    Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    1-81 amino acids from β1,4-galactosyltransferase (B4GALT1 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    87327-plasmid-map-sequence-id-177633

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  48. pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCP

    Plasmid
    #85165
    Purpose
    Provides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.
    Depositor
    Robin Dowell
    Publication
    Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71
    Insert
    EcoRI-RTX and EcoRI Methylase-AmilCP coding sequences
    Tags
    AmilCP and RTX
    Expression
    Bacterial
    Promoter
    J23100 promoter
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    85165-plasmid-map-sequence-id-354765

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  49. Dazl-CPG-TV

    Plasmid
    #70144
    Purpose
    a targeting vector, to target the endogenous DAZL CPG island with Snrp-GFP-polyA
    Depositor
    Rudolf Jaenisch
    Publication
    Stelzer et al Cell. 2015 Sep 24;163(1):218-29. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    5'hDAZL-snrp-GFP-polyA-pgK-PURO-3'hDAZL
    Use
    Mouse Targeting and Synthetic Biology
    Available since
    Dec. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    70144-plasmid-map-sequence-id-358134

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Showing: 11851 - 11900 of 177138 results