We narrowed to 1,800 results for: PORA

1. pDN34

   Plasmid

   #61343

   Purpose

   mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/PKIt NES)

   Depositor

   Barbara Di Ventura, Roland Eils

   Article

   Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

   Insert

   mCherry
   

   Use

   Tags

   GS linker-AsLov2-biNLS2 and PKIt NES

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAD/CMV/V5-DEST-Cdc42-2G

   Plasmid

   #68812

   Purpose

   FRET-based biosensor reporting on endogenous Cdc42 activation (for Adenovirus production)

   Depositor

   Olivier Pertz

   Article

   Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

   Insert

   Cdc42 activation reporter
   

   Use

   Adenoviral

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. CMV-mEGFP-PKCa-C1

   Plasmid

   #112264

   Purpose

   ITRACKalpha Donor Construct

   Depositor

   Ryohei Yasuda

   Article

   Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

   Insert

   protein kinase C alpha (PRKCA Bovine)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   A11V (please see depositor comments below)

   Promoter

   CMV

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST

   Plasmid

   #105448

   Purpose

   Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites in a viral vector

   Depositor

   Hillel Adesnik

   Article

   Pegard et al Nat Commun. 2017 Oct 31;8(1):1228. doi:

   Has Service

   AAV9

   Insert

   ChrimsonR-mRuby2-ST
   

   Use

   AAV

   Tags

   mRuby2 and soma targeting motif

   Expression

   Mutation

   K176R in Chrimson

   Promoter

   hSyn

   Available since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMRX-IP/SECFP-hATG9A

   Plasmid

   #58995

   Purpose

   Expresses SECFP-hATG9A in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

   Insert

   Homo sapiens autophagy related 9A (PPARG Human)
   

   Use

   Retroviral

   Tags

   Venus

   Expression

   Mutation

   Promoter

   Available since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-ChRmine-mRuby2-ST

   Plasmid

   #227777

   Purpose

   Cation channelrhodopsin ChRmine fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic excitation

   Depositor

   Hillel Adesnik

   Article

   Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

   Insert

   ChRmine-mRuby2-ST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pConRef-2H12

   Plasmid

   #221182

   Purpose

   cdGreen expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11213)

   Depositor

   Urs Jenal

   Article

   Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

   Inserts

   cdGreen
   mScarlet-I
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N-terminus changed: starts with MSKKYGEAVIKE ...

   Promoter

   Constitutive and None (same as cdGreen)

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac-Dual_FluA/H7N9

   Plasmid

   #229860

   Purpose

   pFastBac Dual vector for co-expression of FluPolA/H7N9 polymerase heterotrimer (PA/PB1/PB2)

   Depositor

   Stephen Cusack

   Article

   Kouba et al Cell Rep. 2023 Jan 31;42(1):111901. doi:

   Inserts

   PA_H7N9-FluA
   PB1_H7N9-FluA
   PB2_H7N9-FluA
   

   Use

   Tags

   2x-Streptag and 8xHis-tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAL175

   Plasmid

   #22276

   Purpose

   Expresses YFP-PIF for red-light-induced binding to Phy

   Depositor

   Christopher Voigt

   Article

   Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

   Insert

   PIF6APB (PIL2 Mustard Weed)
   

   Use

   Synthetic Biology

   Tags

   mYFP

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGFP-LacI

    Plasmid

    #179510

    Purpose

    Expresses EGFP-LacI in mammalian cells

    Depositor

    Yasushi Hiraoka

    Article

    Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

    Insert

    LacI
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMRXIP-GFP-FAPP(PHD)

    Plasmid

    #221748

    Purpose

    Stable expression of GFP-FAPP(PHD) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    FAPP (PLEKHA3 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    amino acids 1–101

    Promoter

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBabe-Puro-RhoA FLARE.sc Biosensor

    Plasmid

    #12602

    Depositor

    Klaus Hahn

    Article

    Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

    Insert

    RhoA Biosensor (RHOA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAVS1-PuroR Xlone eGFP

    Plasmid

    #179837

    Purpose

    Doxycycline-inducible expression of eGFP

    Depositor

    Xiaoping Bao

    Article

    Jung et al ACS Synth Biol. 2022 May 24. doi: 10.102

    Insert

    eGFP
    

    Use

    CRISPR, Synthetic Biology, and TALEN ; Donor plas…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GS

    Available since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLV-EF1a-MinE(L4E)-EGFP_IRES-Blast

    Plasmid

    #213117

    Purpose

    Expression of a faster-running MinE mutant in mammalian cells

    Depositor

    Scott Coyle

    Article

    Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

    Insert

    MinE(L4E)-EGFP (minE Escherichia coli)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    L4E

    Promoter

    EF1a

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Ef1a-DIO-OMOR-eYFP

    Plasmid

    #66847

    Purpose

    Expresses Opto-MOR in a Cre dependent manner

    Depositor

    Michael Bruchas

    Article

    Siuda et al Neuron. 2015 May 20;86(4):923-35. doi: 1

    Insert

    Opto Mu opioid receptor
    

    Use

    AAV and Cre/Lox

    Tags

    eYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-EF1a-FRB-mCherry-MinD-IRES-Puro

    Plasmid

    #213116

    Purpose

    Expression of FRB-mCherry-MinD in mammalian cells for payload delivery

    Depositor

    Scott Coyle

    Article

    Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

    Insert

    FRB-mCherry-MinD (minD Escherichia coli)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CMV-PKCa-mEGFP-N1

    Plasmid

    #112269

    Purpose

    IDOCKSalpha Donor Construct

    Depositor

    Ryohei Yasuda

    Article

    Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

    Insert

    Protein Kinase C alpha (PRKCA Bovine)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    A11V (please see depositor comments below)

    Promoter

    CMV

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-Dlx-FLEX-hM4Di-mCherry

    Plasmid

    #209701

    Purpose

    Expresses hM4Di-mCherry under the control of distalless homeobox 5 and 6 (mDlx) enhancer in Cre-recombinase-dependent manner

    Depositor

    Peer Wulff

    Article

    Rangel Guerrero et al Neuron. 2024 Apr 10:S0896-6273(24)00197-

    Insert

    hM4D(Gi)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-GfaABC1D-GRAB_eCB2.0

    Plasmid

    #164609

    Purpose

    Express the endocannabinoid sensor GRAB_eCB2.0 in astrocytes

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid sensor GRAB_eCB2.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    S383T

    Promoter

    GfaABC1D

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDN80

    Plasmid

    #61349

    Purpose

    LINuS-flex: modular LINuS construct enabling introduction of any protein encoding sequence, NES and NLS. The sequence of interest replaces the LexA DBD-MBP-VP64 fragment.

    Depositor

    Barbara Di Ventura, Roland Eils

    Article

    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    AsLOV2 wt and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMIR-His-Halo-Tev-PTEN

    Plasmid

    #58241

    Purpose

    expresses halo tagged PTEN protein

    Depositor

    Yimon Aye

    Article

    Fang et al J Am Chem Soc. 2013 Oct 2;135(39):14496-

    Insert

    phosphatase and tensin homolog (PTEN Human)
    

    Use

    Tags

    His-Halo-Tev

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDB22

    Plasmid

    #61342

    Purpose

    Mammalian expression of mCherry fused to LINuS, a light-inducible nuclear localization signal (cMycP1A NLS/PKIt NES)

    Depositor

    Barbara Di Ventura, Roland Eils

    Article

    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

    Insert

    mCherry
    

    Use

    Tags

    GS linker-AsLov2-cMyc1A NLS and PKIt NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pX330 EGFR-sgRNA

    Plasmid

    #188633

    Purpose

    A human codon-optimized SpCas9 and chimeric guide RNA targeting C-terminus of human EGFR

    Depositor

    Jared Toettcher

    Article

    Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. M249-5'csr-1-Pgsy-1-luciferase-Bar-3'csr-1

    Plasmid

    #89693

    Purpose

    Luciferase reporter plasmid targeted at csr-1 locus

    Depositor

    Christian Hong

    Article

    Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

    Insert

    gsy-1 promoter, luciferase
    

    Use

    Yeast episomal vector

    Tags

    luciferase

    Expression

    Mutation

    Promoter

    gsy-1

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3-AktAR-Kras

    Plasmid

    #123357

    Purpose

    FRET-based biosensor for monitoring Akt activity in non-raft plasma membrane regions.

    Depositor

    Jin Zhang

    Article

    Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

    Insert

    AktAR-Kras
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV-TM-CIBN-BLITz1-TetR-VP16

    Plasmid

    #89878

    Purpose

    expresses TM-CIBN-BLITz1-TetR-VP16 of BLITz system

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    TM-CIBN-TevN-BLITz1-TetR-VP16
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AAV-syn-ChR2-Kv-P2A-H2b-mRuby2

    Plasmid

    #89570

    Purpose

    Channelrhodopsin localized to neuronal soma with simultaneous nuclear labeling

    Depositor

    McLean Bolton

    Article

    Baker et al Elife. 2016 Aug 15;5. pii: e14193. doi:

    Inserts

    Channelrhodopsin
    Histone 2B
    

    Use

    AAV

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    synapsin

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV-NES-CRY2PHR-TevC

    Plasmid

    #89877

    Purpose

    expresses NES-CRY2PHR-TevC of BLITz system

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    NES-CRY2PHR-TevC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDP085

    Plasmid

    #198425

    Purpose

    pUBQ10:BirA. A pGreen based plasmid expressing E. coli BirA after the Arabidopsis pUBQ10 promoter. This plasmid is originated from You et al., Nat Commun. 2017, doi: 10.1038/ncomms15120

    Depositor

    Markus Schmid

    Article

    You et al Nat Commun. 2017 May 17;8:15120. doi: 10

    Insert

    E. coli BirA
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pUBQ10

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcPylRS(Y384F Y306A)

    Plasmid

    #196486

    Purpose

    To express Methanosarcina mazei PylRS(Y384F Y306A) and allow UAG codon to be translated into Z-Lys or Azido-Z-Lys when used together with the pU6tRNAPyl plasmid.

    Depositor

    Kensaku Sakamoto

    Article

    Hino et al Methods Mol Biol. 2012;794:215-28. doi:

    Insert

    pyrrolysyl-tRNA synthetase variant with Y384F Y306A mutations
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSNK

    Plasmid

    #135193

    Purpose

    Arabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains kamamycin resistance cassette

    Depositor

    Hongqing Fang

    Article

    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

    Insert

    I-SceI lamba Red
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    araBAD

    Available since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAH34 (Phsp-16.41::cGAL-N::let-858 3'UTR)

    Plasmid

    #107133

    Purpose

    cGAL-N driver under the control of C. elegans hsp-16.41 promoter

    Depositor

    Paul Sternberg

    Article

    Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

    Insert

    NLS::cGAL(DBD)::gp41-1-N-intein
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    C. elegans hsp-16.41 promoter

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pUBC_V5_MS2-mVenus

    Plasmid

    #107606

    Purpose

    Transient expression of humanized MS2 (V75E/A81G), fused to mVenus in mammalian cells, under a hUBC promoter, V5 tag

    Depositor

    John Rinn

    Article

    Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

    Insert

    MS2-mVenus
    

    Use

    Synthetic Biology

    Tags

    V5 and mVenus

    Expression

    Mutation

    MS2: V75E/A81G

    Promoter

    human UBC

    Available since

    May 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pDN42

    Plasmid

    #61345

    Purpose

    mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS10/PKIt NES)

    Depositor

    Barbara Di Ventura, Roland Eils

    Article

    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

    Insert

    mCherry
    

    Use

    Tags

    GS linker-AsLov2-biNLS10 and PKIt NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAL188

    Plasmid

    #22277

    Purpose

    Expresses Tiam DH-PH domains fused to mYFP-PIF6, for optogenetic recruitment

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    TiamDHPH-20aaLinker-mYFP-PIF6APB (PIL2 Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLV-EF1a-PKAsub-mCherry-MinD-IRES-Puro

    Plasmid

    #213118

    Purpose

    Expression of one half of a PKAsub-mCherry-MinD reporter circuit in mammalian cells

    Depositor

    Scott Coyle

    Article

    Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

    Insert

    PKAsub-mCherry-MinD (minD Escherichia coli)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. p2H12Matry-blind

    Plasmid

    #221181

    Purpose

    cdGreen-Matry(blind) expressed from an aTc-inducible promoter (Internal Lab ID: UJ11209)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Insert

    cdGreen-Matry(blind)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. JW617

    Plasmid

    #64809

    Purpose

    CUC3 expression in yeast

    Depositor

    Detlef Weigel

    Article

    Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

    Insert

    CUC3 (CUC3 Mustard Weed)
    

    Use

    Tags

    GAL4 DNA binding domain and Myc

    Expression

    Mutation

    Promoter

    Available since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGTag-P2A-QFGal4-SV40pA

    Plasmid

    #155127

    Purpose

    Donor for precise CRISPR directed genomic integration of QFGal4 using the GeneWeld method

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    P2A-QFGal4
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. JW624

    Plasmid

    #64808

    Purpose

    CUC2 expression in yeast

    Depositor

    Detlef Weigel

    Article

    Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

    Insert

    CUC2 (CUC2 Mustard Weed)
    

    Use

    Tags

    GAL4 DNA binding domain

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge