159,352 results
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Plasmid#59070PurposeAAV-mediated expression of CoChR-GFP under the Syn promoterDepositorInsertCoChR-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterSynAvailable sinceAug. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
p11d-tRPA(123)
Plasmid#102613PurposeExpression of human RPA in E. coliDepositorUseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRG/III-hs-MBP-CB2-HF
Plasmid#121682PurposeBacterial expression of cannabinoid receptor CB2 fusion proteinDepositorInsertperipheral cannabinoid receptor (CNR2 Human)
UseTagsHis6 Flag tagExpressionBacterialMutationPromoterlacAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-EGFP-CDMPR
Plasmid#182642PurposeMammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), followed by EGFP and murine CDMPR (without its signal peptide)DepositorInsertCDMPR
UseRetroviralTagsEGFP and N-terminal ER targeting sequence of hema…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-EFS-LSL-nlsGFP-shRNA
Plasmid#175584PurposeConditional express shRNA with control of LoxPDepositorTypeEmpty backboneUseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEFSAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
PLEX_313 SLC31A1
Plasmid#184493PurposeLentivirus expression for SLC31A1 geneDepositorInsertSLC31A1 (SLC31A1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.iGluSnFR3.v857.GPI
Plasmid#178331PurposeFluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPIDepositorHas ServiceAAV1InsertpAAV hSyn iGluSnFR3 v857.GPI
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsGPI Anchor, IgK-chain, and Myc epi tagExpressionMammalianMutationPromoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CHRM3-cpHalo∆-mEGFP
Plasmid#194678PurposeCMV driven expression of the m3 GPCR recorder in mammalian cell lines fused to mEGFPDepositorInsertCHRM3_cpHalo∆
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCDH-METTL16-3*Flag
Plasmid#196207Purposeoverexpress METTL16DepositorInsertWT METTL16 (METTL16 Human)
UseLentiviralTagsExpressionMutationNOPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKPI-MtLAP1
Plasmid#193521PurposeAnthocyanin pigmentation based visual marker for arbuscular mycorrhizal fungal colonization of Medicago truncatula rootsDepositorInsertsMtLAP1
DsRed
UseTagsExpressionPlantMutationPromoterATUBI10 and Medtr8g059790(KPI)Available sinceJune 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pS548∙CsR
Plasmid#197859PurposeExpresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteriaDepositorInsertgRNA scaffold - Tc resistance cassette - incP oriT
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPEM7Available sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO V5 HSPA2
Plasmid#19512DepositorInsertheat shock 70kDa protein 2 (HSPA2 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDuRCC-K
Plasmid#81193PurposeExpression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan ResistanceDepositorInsertsCas9
empty gRNA cassette
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease see depositor comments below., Please view…PromoterROX3 and SNP52pAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO-shp38
Plasmid#52920Purposeexpresses a hairpin specific for human p38MAPKDepositorInsertshRNA targeting mitogen-activated protein kinase p38 (MAPK14 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceMay 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
TFORF1938
Plasmid#141892PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMEIS1 (MEIS1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV3flag8HOIP
Plasmid#50015PurposeMammalian expression of human HOIP (RNF31) with a c-terminal 3xFLAG tag, and hygromycin selection.DepositorInsertring finger protein 31 (RNF31 Human)
UseTags3 x FLAGExpressionMammalianMutationPromoterCMVAvailable sinceDec. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSL21-mCherry
Plasmid#164410PurposeRetroviral expression plasmid of sgRNA with mCherryDepositorTypeEmpty backboneUseCRISPR and RetroviralTagsExpressionMammalianMutationPromoterU6 promoter for crRNA expression and EFS promoter…Available sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3b-8XGliBS:EGFP
Plasmid#84602PurposeGli protein-dependent EGFP reporter for analysis of Hedgehog signalingDepositorInserteGFP
UseTagsExpressionMammalianMutationPromoter8X Gli-binding siteAvailable sinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA GNSTM-3-Flag-10-Lamp2b-HA
Plasmid#71293PurposeEncodes (N to C): GNSTM glycosylation motif, 3 residue spacer, Flag tag,10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.DepositorInsertLamp2b (LAMP2 Human)
UseTagsFLAG, GNSTM glycosylation motif, HA, and Lamp2 si…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR-SYNZIP18
Plasmid#80672PurposeEncodes the synthetic coiled coil peptide SYNZIP18DepositorInsertSYNZIP18
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceMay 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
SpRY Cas9
Plasmid#188491PurposeExpresses FLAG tagged SpRY Cas9DepositorInsertCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationA61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…PromoterEF1aAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
DsRed2-Peroxisomes-4
Plasmid#54503PurposeLocalization: Peroxisomes, Excitation: 558, Emission: 583DepositorTypeEmpty backboneUseTagsDsRed2 and Peroximal Targeting Signal 1 (PTS1)ExpressionMammalianMutationPromoterAvailable sinceJune 11, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
p6sheLLr
Plasmid#37318DepositorInsertHPV6L1+L2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV:DsRed(FRT)GFP
Plasmid#31128DepositorInsertDsRed(FRT)GFP
UseFlp/frtTagsExpressionMammalianMutationPromoterAvailable sinceOct. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHPRT-DRGFP
Plasmid#26476PurposeDR-GFP substrate (Addgene 26475) flanked by mouse hprt targeting sequencesDepositorInsertpHPRT-DRGFP
UseMouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-AckRS
Plasmid#137976Purposesequence optimized N‐acetyl lysyl‐tRNA synthetase with cognate tRNA for genetic code expansionDepositorInsertAckRS and pylTcua
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
EB3N-VVDfast-Halo-iLID
Plasmid#190166PurposeExpresses EB3N-VVDfast-Halo-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.DepositorInsertEB3N-VVDfast-Halo-iLID
UseTags-VVDfast-mClover3-iLIDExpressionMammalianMutation1-189 aa from EB3PromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCHC042 (∆phaCAB)
Plasmid#226208PurposeConjugation knockout plasmid (pK18msB) to delete the C. necator phaCAB operon, to prevent accumulation of PHB.DepositorInsertHomology arms for phaCAB
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
hCtRNA_FT
Plasmid#186715PurposeFragment template for DAP arrayDepositorInsertgRNA scaffold and hCtRNA
UseCRISPRTagsExpressionMammalianMutationPromoterhCtRNAAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
hKCNQ3(WT).ires.mCherry-pcDNA3
Plasmid#204362PurposeHeterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)DepositorInsertKCNQ3 (KCNQ3 Human)
UseTagsExpressionMammalianMutationPromoterCMV/T7 RNApolAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
plRL2
Plasmid#163631PurposeOptimized M. tuberculosis CRISPRi plasmid; L5 integrating.DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterTet-OnAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-hPIKfyve
Plasmid#121148PurposeExpresses GFP-tagged human PIKfyve in mammalian cells.DepositorInsert1-phosphatidylinositol 3-phosphate 5-kinase isoform 2 (PIKFYVE Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
EpsilonCOP EGFP
Plasmid#128151PurposeExpression of epsilon COPDepositorInsertepsilon COP (COPE Bovine)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-GFP-RELA
Plasmid#196629PurposeExpress GFP-RELADepositorInsertGFP-RELA (RELA Human)
UseLentiviralTagsGFPExpressionMutationWTPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pK170.AAV-TRE-Cre-WPRE (Supernova)
Plasmid#85040PurposeFor AAV-TRE-Cre based Supernova, pK170 should be used with pK168 etc.DepositorInsertnlsCre-WPRE
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tet-pLKO-FOXM1-shRNA-81
Plasmid#89497PurposeLentiviral vector with dox inducible expression of shRNA targeting human FOXM1.DepositorInsertFOXM1 (FOXM1 Human)
UseLentiviral and RNAi; Doxycycline inducibleTagsExpressionMammalianMutationPromoterH1/TOAvailable sinceJune 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEM4Z-OMA1
Plasmid#61718Purposehuman Oma1 protease in the E.coli/in vitro translation vectorDepositorInsertOMA1 (OMA1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV_FR-GECO1c
Plasmid#224358PurposeExpresses red fluorescent calcium sensor FR-GECO1cDepositorInsertFR-GECO1c
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFP5370 ss-FIREmate-HA- KDEL-IVS-IRES- TNF-SBP- mCherry-FIREtag
Plasmid#216696PurposeSynchronize trafficking of TNFalpha from the ER (RUCH system)DepositorInsertsFIREmate-KDEL
Tumor Necrosis Factor alpha (TNFa)
UseTagsFIREtag, Streptavidin Binding Protein (SBP), and …ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
Plasmid L5 attB::Pleft* mScarlet
Plasmid#169410PurposeChromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mScarletDepositorInsertspLeft
mScarlet proper
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only